Biological synthesis of silver nanoparticles from leaf extract of Piper betel and its antibacterial activities

2020 ◽  
Author(s):  
Hannah Cynthia Samuel ◽  
Saraswathy Nachimuthu ◽  
Balaji Sadhasivam ◽  
Ramalingam Ponnusamy
Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 299
Author(s):  
Reetika Singh ◽  
Christophe Hano ◽  
Gopal Nath ◽  
Bechan Sharma

Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.


Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4479
Author(s):  
Pei-Jun Li ◽  
Jiang-Juan Pan ◽  
Li-Jun Tao ◽  
Xia Li ◽  
Dong-Lin Su ◽  
...  

The present study focuses on the biological synthesis, characterization, and antibacterial activities of silver nanoparticles (AgNPs) using extracellular extracts of Aspergillus japonicus PJ01.The optimal conditions of the synthesis process were: 10 mL of extracellular extracts, 1 mL of AgNO3 (0.8 mol/L), 4 mL of NaOH solution (1.5 mol/L), 30 °C, and a reaction time of 1 min. The characterizations of AgNPs were tested by UV-visible spectrophotometry, zeta potential, scanning electron microscope (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and thermogravimetric (TG) analyses. Fourier transform infrared spectroscopy (FTIR) analysis showed that Ag+ was reduced by the extracellular extracts, which consisted chiefly of soluble proteins and reducing sugars. In this work, AgNO3 concentration played an important role in the physicochemical properties and antibacterial properties of AgNPs. Under the AgNO3 concentration of 0.2 and 0.8 mol/L, the diameters of AgNPs were 3.8 ± 1.1 and 9.1 ± 2.9 nm, respectively. In addition, smaller-sized AgNPs showed higher antimicrobial properties, and the minimum inhibitory concentration (MIC) values against both E. coli and S. aureus were 0.32 mg/mL.


2021 ◽  
Vol 19 ◽  
Author(s):  
Moinuzzaman ◽  
Mohammad Abu Hena Mostofa Jamal ◽  
Helal Uddin ◽  
Shahedur Rahman ◽  
Abdul Momin ◽  
...  

Background: In new modern science Nanotechnology is an emerging field for the researchers. Nanotechnology deals with the Nanoparticles. These Nanoparticles have a size of 1-100 nm in one dimension which are important part of biomedical science and medical chemistry, atomic physics, and all other known fields. Nanoparticles are used in broad range due to its small size, orientation, physical properties. Objectives: This study was designed to synthesized Silver nanoparticles using Azadirachta indica (Neem) leaf extract and evaluate biomedical application. Methods: Aqueous extract of Azadirachta indica is used for the synthesis of AgNPs. 1ml of the extract was added to (30- 60) ml of 1mM solution of silver nitrate drop by drop. Silver nanoparticle was characterized by UV-Visible Spectroscopy, Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering Spectroscopy, X-ray Diffraction, Transmission Electron Microscopy, Antibacterial activity studies. Results: The Biological synthesis of Silver nanoparticles (AgNPs) was done by using the aqueous solution of Azadirachta indica leaf extract and AgNO3. A fixed ratio of plant extract to metal ion was used to prepare AgNPs and the formation of the nanoparticles was observed by the color change. The nanoparticles were characterized by UV-vis Spectrophotometer, FTIR, DLS, XRD and TEM. The nanoparticles were found have the size ranges from 30-60 nm. The biosynthesized silver nanoparticles had bactericidal effect against antibiotic resistance pathogenic microorganisms such as Bascillus subtilis, pseudomonas aeruginosa and Bascillus cereus. Conclusion: Silver nanoparticles were synthesized by Azadirachta indica leaf extract can be used as a therapeutic candidate for biomedical applications.


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