Green Biosynthesis of Silver Nanoparticles Using Leaf Extract of Carissa carandas L. and Their Antioxidant and Antimicrobial Activity against Human Pathogenic Bacteria

Carissa carandas L. is traditionally used as antibacterial medicine and accumulates many antioxidant phytochemicals. Here, we expand this traditional usage with the green biosynthesis of silver nanoparticles (AgNPs) achieved using a Carissa carandas L. leaf extract as a reducing and capping agent. The green synthesis of AgNPs reaction was carried out using 1mM silver nitrate and leaf extract. The effect of temperature on the synthesis of AgNPs was examined using room temperature (25 °C) and 60 °C. The silver nanoparticles were formed in one hour by stirring at room temperature. In this case, a yellowish brown colour was developed. The successful formation of silver nanoparticles was confirmed by UV–Vis, Fourier transform infrared (FT-IR) and X-ray diffraction (XRD) analysis. The characteristic peaks of the UV-vis spectrum and XRD confirmed the synthesis of AgNPs. The biosynthesised AgNPs showed potential antioxidant activity through DPPH assay. These AgNPs also exhibited potential antibacterial activity against human pathogenic bacteria. The results were compared with the antioxidant and antibacterial activities of the plant extract, and clearly suggest that the green biosynthesized AgNPs can constitute an effective antioxidant and antibacterial agent.

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Antibacterial activity of silver Nanoparticles produced by Rosmarinus officinalis L leaf extract against some human pathogenic bacteria

International Journal of Advanced Biological and Biomedical Research ◽

10.18869/ijabbr.2016.108 ◽

2016 ◽

Vol 4 (1) ◽

pp. 108-111 ◽

Cited By ~ 1

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Leaf Extract ◽

Rosmarinus Officinalis ◽

Human Pathogenic Bacteria ◽

Rosmarinus Officinalis L

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Expedient Synthesis and Antibacterial Activity of Tetrahydro-1′H-spiro[indoline-3,4′-quinoline]-3′-carbonitrile Derivatives Using Piperidine as Catalyst

Synlett ◽

10.1055/s-0040-1706682 ◽

2021 ◽

Author(s):

Appaswami Lalitha ◽

Nangagoundan Vinoth ◽

Pullar Vadivel

Keyword(s):

Antibacterial Activity ◽

Pathogenic Bacteria ◽

Room Temperature ◽

Antibacterial Activities ◽

Spectroscopic Techniques ◽

Human Pathogenic Bacteria ◽

Convenient Synthesis

AbstractA convenient synthesis of 2′-amino-7′,7′-dimethyl-2,5′-dioxo-1′-(phenylamino)-5′,6′,7′,8′-tetrahydro-1′H-spiro[indoline-3,4′-quinoline]-3′-carbonitrile derivatives has been designed using different substituted isatins, various 5,5-dimethyl-3-(2-phenylhydrazinyl)cyclohex-2-enones (arylhydrazones of dimedone) and malononitrile in ethanol with piperidine as catalyst at room temperature. The structures of the synthesized compounds have been elucidated by various spectroscopic techniques. The selected compounds have also been evaluated for their antibacterial activities against human pathogenic bacteria.

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Ecofriendly Synthesis of Silver Nanoparticles Using Ethno Medicinal Plant Leaf Extract (Jasminum auriculatum) and their Antibacterial Properties

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.58.113 ◽

2015 ◽

Vol 58 ◽

pp. 113-121 ◽

Cited By ~ 3

Author(s):

S. Balasubramanian ◽

U. Jeyapaul ◽

S. Mary Jelastin Kala

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Antibacterial Properties ◽

Cost Effective ◽

Average Diameter ◽

Xrd Analysis ◽

Antibacterial Activities ◽

Aqueous Leaf Extract ◽

Plant Leaf Extract

The present investigation, we describe a cost effective biofabrication of silver nanoparticles using jasminum auriculatum leaf extract. The aqueous leaf extract of jasminum auriculatum are used as reducing agent and capping agent in silver nanoparticles synthesis. The biofabricated silver nanoparticles were characterized by UV-Visible spectrophotometer, X-Ray Diffraction, FTIR spectra and SEM with EDX analysis. FTIR studies revealed that the functional group responsible for silver reduction were found to be jasminol and triterpinoid compounds present in leaf extract. XRD analysis showed that prepared silver nanoparticles are crystalline in nature with an average diameter of 15-20 nm. The prepared silver nanoparticles have been evaluated in vitro for antibacterial activities and found to have higher antibacterial activities.

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Facile Green, Room-Temperature Synthesis of Gold Nanoparticles Using Combretum erythrophyllum Leaf Extract: Antibacterial and Cell Viability Studies against Normal and Cancerous Cells

Antibiotics ◽

10.3390/antibiotics10080893 ◽

2021 ◽

Vol 10 (8) ◽

pp. 893

Author(s):

Olufunto T. Fanoro ◽

Sundararajan Parani ◽

Rodney Maluleke ◽

Thabang C. Lebepe ◽

Jose R. Varghese ◽

...

Keyword(s):

Gold Nanoparticles ◽

Cell Viability ◽

Leaf Extract ◽

Room Temperature ◽

Klebsiella Oxytoca ◽

Cost Effective ◽

Average Diameter ◽

Antibacterial Activities ◽

Inhibition Concentration ◽

First Time

We herein report a facile, green, cost-effective, plant-mediated synthesis of gold nanoparticles (AuNPs) for the first time using Combretum erythrophyllum (CE) plant leaves. The synthesis was conducted at room temperature using CE leaf extract serving as a reducing and capping agent. The as-synthesized AuNPs were found to be crystalline, well dispersed, and spherical in shape with an average diameter of 13.20 nm and an excellent stability of over 60 days. The AuNPs showed broad-spectrum antibacterial activities against both pathogenic Gram-positive (Staphylococcus epidermidis (ATCC14990), Staphylococcus aureus (ATCC 25923), Mycobacterium smegmatis (MC 215)) and Gram-negative bacteria (Proteus mirabilis (ATCC 7002), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13822), Klebsiella oxytoca (ATCC 8724)), with a minimum inhibition concentration of 62.5 µg/mL. In addition, the as-synthesized AuNPs were highly stable with exceptional cell viability towards normal cells (BHK- 21) and cancerous cancer cell lines (cervical and lung cancer).

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Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Kluwak Pangium edule Reinw Sebagai Bioreduktor dan Uji Aktivitasnya Sebagai Antioksidan

Jurnal Ilmu Alam dan Lingkungan ◽

10.20956/jal.v10i1.6557 ◽

2019 ◽

Vol 10 (1) ◽

Author(s):

Indrawati Patabang ◽

Syahruddin Kasim ◽

Paulina Taba

Keyword(s):

Antioxidant Activity ◽

Particle Size ◽

Silver Nanoparticles ◽

Leaf Extract ◽

Average Particle Size ◽

Xrd Analysis ◽

Average Particle ◽

Result Show ◽

Ic50 Values ◽

Show Result

Silver nanoparticles have been synthesized using kluwak leaf extract (Pangium edule Reinw) as bioreductor and antioxidant activity assay. The nanoparticles formed were monitored by observing UV-Vis absorption and characterized by using FTIR, PSA, XRD and SEM instruments. The result of functional group characterization with FTIR show that the functional groups OH, C = O, C-O and CH2 act as Ag+ reducing agent. The size of silver nanoparticles was determined by using Particle Size Analyzer (PSA) and the result show average particle size distribution of 93.2 nm. Morphology of AgNp were observed by Scanning Electron Microscope (SEM) and X-Ray Difraction (XRD) analysis show result of 51,78 nm. The antioxidant activity was shown by in kluwak leaf extract and silver nanoparticles with IC50 values respectively 831,33 ppm dan 1493,09 ppm.

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Size-Controlled Green Synthesis of Silver Nanoparticles using Dual Functional Plant Leaf Extract at Room Temperature

International Journal of Green Nanotechnology ◽

10.1080/19430892.2012.706183 ◽

2012 ◽

Vol 4 (3) ◽

pp. 310-325 ◽

Cited By ~ 3

Author(s):

Yekkuni L. Balachandran ◽

Pazhanisami Peranantham ◽

Rajendran Selvakumar ◽

Arno C. Gutleb ◽

Shanmugam Girija

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Leaf Extract ◽

Room Temperature ◽

Plant Leaf ◽

Dual Functional ◽

Plant Leaf Extract ◽

Size Controlled

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Ultra-sonication enhanced green synthesis of silver nanoparticles using Barleria buxifolia leaf extract and its possible application

10.21203/rs.3.rs-161063/v1 ◽

2021 ◽

Author(s):

Vanaraj sekar

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Leaf Extract ◽

Cancer Cell Line ◽

Xrd Analysis ◽

Blue Dye ◽

Multiple Use ◽

Uniform Size ◽

X Ray ◽

Vis Spectroscopy

Abstract A simple and eco-friendly method for the green synthesis of silver nanoparticles (AgNPs) by ultrasound-assisted strategy using Barleria buxifolia leaf extract as a reducing and capping agent was established in this study. The obtained AgNPs were characterized. UV-vis spectrum, Fourier transform infrared spectroscopy (FTIR), scanning and transmission electron microscopy (SEM and TEM), Energy Dispersive X-Ray Analyzer (EDX), X-ray diffraction, dynamic light scattering (DLS) analysis showed that the obtained AgNPs were mono dispersed spheres with uniform size of 80 nm. UV-vis spectroscopy, FTIR, and XRD analysis indicated that the surface of the obtained AgNPs was covered with organic molecules in plant extracts. The results of ABTS assays showed that high antioxidant activity was seen in the obtained AgNPs. Green synthesized AgNPs showed potent antibacterial and anti-biofilm activity against tested pathogens. Cytotoxicity assay showed that the obtained AgNPs were significantly cytotoxic to cancer cell line (MCF-7). In addition, the AgNPs synthesized in this paper can also photo catalytically degrade methylene blue dye under visible light. The potent bioactivity exhibited by the green synthesized silver nanoparticles leads towards the multiple use as antioxidant, antibacterial, anti-biofilm, cytotoxic as well as photo catalytic agent.

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