Biogenic synthesis of Silver Nanoparticles using Azadirachta indica (Neem) leaf extract targeting inhibitory action against antibiotic resistance bacteria

2021 ◽  
Vol 19 ◽  
Author(s):  
Moinuzzaman ◽  
Mohammad Abu Hena Mostofa Jamal ◽  
Helal Uddin ◽  
Shahedur Rahman ◽  
Abdul Momin ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibiotic Resistance ◽  
Azadirachta Indica ◽  
Metal Ion ◽  
Leaf Extract ◽  
Biomedical Application ◽  
Fixed Ratio ◽  
Biomedical Science ◽  
Biological Synthesis ◽  
Neem Leaf Extract

Background: In new modern science Nanotechnology is an emerging field for the researchers. Nanotechnology deals with the Nanoparticles. These Nanoparticles have a size of 1-100 nm in one dimension which are important part of biomedical science and medical chemistry, atomic physics, and all other known fields. Nanoparticles are used in broad range due to its small size, orientation, physical properties. Objectives: This study was designed to synthesized Silver nanoparticles using Azadirachta indica (Neem) leaf extract and evaluate biomedical application. Methods: Aqueous extract of Azadirachta indica is used for the synthesis of AgNPs. 1ml of the extract was added to (30- 60) ml of 1mM solution of silver nitrate drop by drop. Silver nanoparticle was characterized by UV-Visible Spectroscopy, Fourier Transform Infrared Spectroscopy, Dynamic Light Scattering Spectroscopy, X-ray Diffraction, Transmission Electron Microscopy, Antibacterial activity studies. Results: The Biological synthesis of Silver nanoparticles (AgNPs) was done by using the aqueous solution of Azadirachta indica leaf extract and AgNO3. A fixed ratio of plant extract to metal ion was used to prepare AgNPs and the formation of the nanoparticles was observed by the color change. The nanoparticles were characterized by UV-vis Spectrophotometer, FTIR, DLS, XRD and TEM. The nanoparticles were found have the size ranges from 30-60 nm. The biosynthesized silver nanoparticles had bactericidal effect against antibiotic resistance pathogenic microorganisms such as Bascillus subtilis, pseudomonas aeruginosa and Bascillus cereus. Conclusion: Silver nanoparticles were synthesized by Azadirachta indica leaf extract can be used as a therapeutic candidate for biomedical applications.

scholarly journals Azadirachta indica (Neem) Leaf Extract Effect as an Option of Treatment of Ibuprofen-induced Nephrotoxicity

2020 ◽  
pp. 56-62
Author(s):  
Ifeanacho Ezeteonu Abireh ◽  
Onyinye Mary Ozioko ◽  
Ignatius Ikemefuna Ozor ◽  
Elizabeth Finbarrs- Bello ◽  
Uche Sebastine Ozioko ◽  
...  
Keyword(s):  
Serum Creatinine ◽  
Azadirachta Indica ◽  
Leaf Extract ◽  
Kidney Injury ◽  
Wistar Rat ◽  
Male Wistar Rats ◽  
Oral Ibuprofen ◽  
Group 2 ◽  
Neem Leaf Extract ◽  
Group 1

Aim: This study investigated the curative effect of the aqueous leaf extract of Azadirachta indica on Ibuprofen-induced nephrotoxicity in Wistar rat Study Design: This is an experimental research Place of Research: Department of Anatomy, College of Medicine, Enugu State University of Science and Technology. Methodology: Twenty-four male Wistar rats were divided into 6 groups, with 4 rats in each group. Group 1 was control and received oral normal saline 0.5 ml daily. Group 2-6 had induction of nephrotoxicity using oral Ibuprofen 400 mg/Kg daily for 5 days. Group 3-5 were subsequently treated with gavage Azadirachta indica leaf extract 200 mg/Kg, 400 mg/Kg and 800 mg/Kg, respectively, for 5 days. And Group 6 was treated with oral Vitamin E 1000 iu/kg for 5 days. Results: Ibuprofen induced nephrotoxicity as evidenced by elevation of serum creatinine level in group 2 (1.99 ± 0.83), when compared to 0.48 ± 0.07 obtained in group 1 (control), and Bowman’s capsule enlargement with glomerular degeneration observed in group 2. The serum creatinine levels progressively approached the level of that of the control in groups treated with Azadirachta indica leaf extract, groups 3 (1.69 ± 0.52), 4 (0.69 ± 0.10) and 5 (0.49 ± 0.10). Also, the histoarchitecture progressively normalized to that of control with each increase in dose of the extract. Conclusion: Azadirachta indica (neem) leaf extract administration led to the resolution of Ibuprofen-induced kidney injury in this study. Thus, it can serve as a treatment option for kidney injury resulting from ingestion of Ibuprofen, after the identification of the molecule responsible for this effect.

Biological synthesis of GO-MgO nanomaterial using Azadirachta indica leaf extract: A potential bio-adsorbent for removing Cr(VI) ions from aqueous media

2021 ◽  
pp. 108272
Author(s):  
Shraban Kumar Sahoo ◽  
Gagan Kumar Panigrahi ◽  
Manoj Kumar Sahu ◽  
Atia Arzoo ◽  
Jitendra Kumar Sahoo ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Aqueous Media ◽  
Biological Synthesis

Development, Characterization and Anticancer Evaluation of Silver Nanoparticles from Dalbergia sissoo Leaf Extracts

2020 ◽  
Vol 16 (2) ◽  
pp. 145-151
Author(s):  
Aakash Deep ◽  
Mitali Verma ◽  
Rakesh K. Marwaha ◽  
Arun K. Sharma ◽  
Beena Kumari
Keyword(s):  
Silver Nanoparticles ◽  
Anticancer Activity ◽  
Metal Ion ◽  
Color Change ◽  
Fixed Ratio ◽  
Cancer Cell Line ◽  
Dalbergia Sissoo ◽  
Leaf Extracts ◽  
Standard Drug

Aim: The objective behind this present work is the development and characterization of silver nanoparticles from Dalbergia sissoo leaf extracts and the analysis of anticancer activity. Methods: Silver nanoparticles were prepared by using the aqueous solution of Dalbergia sisoo leaf extract and silver nitrate. The formation of nanoparticles was determined by the color change during the preparation of plant extract to metal ion in a fixed ratio. The prepared nanoparticles were then characterized by TEM, FTIR, DLS, XRD, and SEM. Silver nanoparticles were also evaluated for anticancer activity. Results: Synthesized silver nanoparticles were having good anticancer activity against MCF 7 cancer cell line as compared to the standard drug Doxorubicin. Conclusion: The particle size of nanoparticles was found to lie in the range of 10 to 50 nm.

Biological synthesis of silver nanoparticles from leaf extract of Piper betel and its antibacterial activities

2020 ◽  
Author(s):  
Hannah Cynthia Samuel ◽  
Saraswathy Nachimuthu ◽  
Balaji Sadhasivam ◽  
Ramalingam Ponnusamy
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antibacterial Activities ◽  
Biological Synthesis

scholarly journals Protective Effect of Aqueous Crude Extract of Neem (Azadirachta indica) Leaves onPlasmodium berghei-Induced Renal Damage in Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Voravuth Somsak ◽  
Sukanya Chachiyo ◽  
Ubonwan Jaihan ◽  
Somrudee Nakinchat
Keyword(s):  
Protective Effect ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Renal Injury ◽  
Leaf Extract ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Potential Candidate ◽  
Neem Leaves ◽  
Neem Leaf Extract

Malaria is a major public health problem in the world because it can cause of death in patients. Malaria-associated renal injury is associated with 45% of mortality in adult patients hospitalized with severe form of the disease. Therefore, new plant extracts to protect against renal injury induced by malaria infection are urgently needed. In this study, we investigated the protective effect of aqueous crude extract ofAzadirachta indica(neem) leaves on renal injury induced byPlasmodium bergheiANKA infection in mice. ICR mice were injected intraperitoneally with 1 × 107parasitized erythrocytes of PbANKA, and neem extracts (500, 1,000, and 2,000 mg/kg) were given orally for 4 consecutive days. Plasma blood urea nitrogen (BUN) and creatinine levels were subsequently measured. Malaria-induced renal injury was evidenced as marked increases of BUN and creatinine levels. However, the oral administration of neem leaf extract to PbANKA infected mice for 4 days brought back BUN and creatinine levels to near normalcy, and the highest activity was observed at doses of 1,000 and 2,000 mg/kg. Additionally, no toxic effects were found in normal mice treated with this extract. Hence, neem leaf extract can be considered a potential candidate for protection against renal injury induced by malaria.

scholarly journals ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus)

2017 ◽  
Vol 11 (1) ◽  
pp. 27-30
Author(s):  
Yudha Fahrimal ◽  
Siti Maghfirah ◽  
Rinidar Rinidar ◽  
Al Azhar ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Control Group ◽  
Neem Leaf Extract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.

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