Future Clinical Application of β-galactosidase Stabilized by Magnesium oxide Nanoparticles

The present study demonstrates the application of freshly prepared neem leaf extract as a reducing agent for synthesizing magnesium oxide nanoparticles (MgO-NPs). In silico interaction of Aspergillus oryzae β-galactosidase with MgO-NPs was observed by using molecular docking program Dock v.6.5 while the visual analyses and illustration of protein–ligand complex were investigated by utilizing chimera v.1.6.2 and PyMOL v.1.3 softwares. The prepared nanomatrix provided 83% immobilization yield, and broadened the biocatalytic activity of immobilized β-galactosidase at higher pH and temperature ranges. Immobilized β-galactosidase exhibited greater activity even at 5.0% galactose concentration as compared to the soluble enzyme under similar experimental conditions. Hence, the use of green nanotechnology makes the process inexpensive, and therefore, immobilization of these enzymes on such nanoparticles can help to recover the enzyme, which ultimately decreases the cost of process.

Effect of calcitor fertilizer and neem leaf extract concentration on production and quality of siam orange fruits

This research was conducted in Belancan Village, Kintamani District, Bangli Regency, Bali, Indonesia, from December 2020 to July 2021, which aimed to increase production and fruits quality of Siam orange with calcitor fertilizer and concentration of neem leaf extract. The study used a randomized block design, factorial with 2 factors and 3 replications. The first factor was calcitor fertilizer consisting of 4 concentration levels, i.e., 0 cc/l/tree (K0); 2 cc/l/tree (K1), 4 cc/l/tree (K2), and 6 cc/l/tree (K3), while the second factor was neem leaf extract consisting of 3 concentration levels, i.e., 0% (M0), 5% (M1), and 10% (M2). The results showed that the interaction between calcitor fertilizer and neem leaf extract concentration had no significant effect on all observed variables. Calcitor fertilizer with a concentration of 6 cc/l/tree could improve the quality of Siam orange fruit, which was reflected by the increase in the number of fruit per tree (106.44 fruit), weight per fruit (114.08 g), weight of fruit per tree (14 .37 kg), fruit diameter (6.89 cm) and total dissolved solids (11.52 %brix) or increased by 27.56%, 7.54%; 40.74%; 12.58% and 44.72%, respectively, than those of control. While in the treatment of neem leaf extract at a concentration of 10% increase the number of fruit per tree (107.73 fruit), weight per fruit (117.44 g), fruit weight per tree (16.04 kg), fruit diameter (7.58 cm) and total dissolved solids (10.83% brix), or an increase of 69.44%; 18.76%; 78.84% and 42.31%, respectively, than those of control.

Antimicrobial Efficacy of Herbal Root Canal Irrigants and 3% Sodium Hypochlorite against Enterococcus faecalis: An In-vitro Study

Nigella sativa (kalonji) has been used since ancient times as a nutritional supplement and for treating various infections and chronic ailments. As pathogens become resistant to most drugs, kalonji can be used as an alternative compound in modern medicines. The use of herbal extracts as endodontic irrigants might be beneficial as a part of a growing trend to seek natural remedies for dental treatment. Aim: To compare the antibacterial potency of Aqueous Nigella sativa extract, Aqueous Neem leaf extract, and 3% Sodium Hypochlorite. Materials and Methods: Test solutions were tested against E. faecalis (ATCC 29212) to check for their Minimum Inhibitory Concentration (MIC) by double dilution method and Kill time to measure their antimicrobial potency to be used as an intracanal irrigant. Results: The study's limitations show that Aqueous Nigella sativa extract has a better antimicrobial effect than Aqueous Neem leaf extract and 3% Sodium Hypochlorite solution against E. faecalis.

Effect of Seed Priming with Micro-Nutrients, Botanicals on Seedling Characteristics of Sorghum (Sorghum bicolor L. Moench) Var. Paiyur 2

The experiment was conducted in Seed Testing Laboratory, Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj (U.P.) during kharif season 2019-2021, so as to standardize the acceptable seed treatments for sorghum (Paiyur-2).Different seed priming treatments were taken into the study viz., were evaluated T1 – Zinc Sulphate @ 0.5% up to 3hours, T2 – Zinc Sulfate@1% at 4 hours, T3 – Zinc [email protected]% at 6 hours, T4 – Thiourea @ 0.5% at 3 hours, T5 – Thiourea @ 1% at 4 hours, T6 – Thiourea @ 1.5% at 6 hours, T7 – KNO3@ 0.5% at 3 hours, T8 – KNO3 @ 1% at 4 hours, T9 – KNO3 @ 1.5% at 6 hours, T10 – Moringa Leaf extract @ 3% at 8 hours, T11 – Neem leaf extract @ 3% at 8 hours, T12 – Thiourea @0.5% with Zinc Sulphate(ZnSo4) @ 0.5% at 6 hours. During this study it had been found that maximum germination percent, seedling length and vigour attributes was observed when seed treated with T10- Moringa leaf extract @ 3% followed by T9 – KNO3 @ 1.5% and minimum observed in Control (Untreated seed). Seed treatment with Moringa leaf extract @ 3% enhances germination on seedling characters and it is a simple method and no requirement for expensive equipment and chemical might be used for overcoming challenges associated with a poor germination and seedling establishment.

Comparative Effect of Different Treaments in Field Pea (Pisum sativum L.)

A field study was conducted to investigate the various treatments that effect on growth and yield of field pea in RBD (Randomized block design) at Sam Higginbottom University of Agriculture Technology and Science, Prayagraj, Uttar Pradesh during march to May in 2020. The trail consists of 13 treatments combinations. The field pea varieties were used were IPF429. The treatments included T0- control, T1,T2,T3,-Gibberellic acid, T4,T5,T6- Neem leaf extract, T7,T8,T9-ZnSo4, T10T11T12- Naphthalene acetic acid(NAA). All Ten parameters treated with Ga3 shows good results in Yield and shows maximum in field emergence, plant height, Days to 50% flowering, Number of pods, Seed yield per plot, Biological Yield and Harvest index. T0 (Un primed) shows lowest of all treatments. Hence, priming with Gibberellic acid could recommended for pre sowing treatment for field pea.

Identifikasi dan Efektivitas Berbagai Teknik Pengendalian Hama Baru Ulat Grayak Spodoptera frugiperda J. E. Smith pada Tanaman Jagung berbasis PHT- Biointensif

The presence of new pest Spodoptera frugperda J. E. Smith has the potential to reduce corn production, so its existence needs to be controlled. The aim of this research is to obtain control techniques that could reduce the intensity of S. frugiperda pest’s attack. The research was conducted from June to October 2020. The experiment was designed in a randomized block design (RBD) with 9 replications and 3 treatments consisting of non-control (NP), PHT-biointensive (BI), and synthetic pesticides. In BI treatment, neem leaf extract (25 mL/plot) and Beauveria bassiana (6,25 g/plot) were used. Meanwhile, the PS treatment used deltametrin active ingredient (3,125 mL/plot). The volume of spray used was 1,25 L. The results showed that PS control techniques gave the best results in suppressing the intensity of fall armyworm S. frugiperda attacks (0,57%-7,04%) followed by BI treatment (0%-6,89%) and NP (0,97%-8,29%). The highest pest populations overall were found in NP treatment and the lowest in PS treatment. PS treatment provided the highest production result with a cob weight of 18,6 kg/plot (24,81 tons/ha), while BI treatment of 17,00 kg/plot (22,72 tons/ha) and NP of 15,44 kg/plot (20,64 tons/ha). The results of the correlation test between the intensity of pest attacks and production results showed that the higher the pest attack, the lower the harvest weight produced. Therefore, pest control by using synthetic pesticides made from active deltametrin is declared effective in suppressing the intensity of attacks and populations of fall armyworm S. frugiperda and able to provide high production results. Keywords: corn, intensity of attack, Spodoptera frugiperda

Immunomodulatory Activity of Methanol Leaf Extract of Neem (Azadirachta indica Juss) Against Suppressor and Proinflammatory Molecules

Neem plant is rich in bioactive constituents, which make it massively discussed the treatment of various diseases. A study on the immunomodulatory activities of neem is given here. This current work aimed to investigate the effects of neem leaf extract on immunocompetent cells. In vivo experiment was carried out using mice (Mus musculus) Â induced with DMBA, comprising positive control, negative control, and treatments of neem leaf extracts (250, 500, and 1000 ppm). Data obtained from flow cytometric analysis were evaluated using BD Cellquest ProTM software, then statistically analyzed in SPSS version 21. Parametric analysis in one-way ANOVA was performed at a significance level of 5%. The significant difference was compared in the Duncan test. The results showed that administration of neem leaf extracts significantly affected the expression of CD4+, CD8+, CD25+, CD62L, IL-10, and IL-17 cells .Neem leaf extract has immunomodulatory activities by increasing pressure molecules and decreasing pro-inflammatory molecules

Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss) terhadap Struktur Histologis Pankreas Tikus Putih (Rattus norvegicus L.) Hiperglikemia

Indikator klinis penyakit Diabetes Melitus adalah hiperglikemia. Mimba (Azadirachta indica A. Juss) merupakan salah satu tumbuhan yang berpotensi sebagai alternatif obat herbal hiperglikemia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol daun mimba terhadap struktur histologis pankreas pada tikus hiperglikemia. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) dengan jumlah tikus 24 ekor yang dibagi menjadi 6 kelompok perlakuan dan 4 kali ulangan. P0 (kontrol normal) adalah kelompok tikus normal yang diberi akuades, P1 (kontrol negatif) adalah tikus hiperglikemia yang diberi akuades. P2 (kontrol positif) adalah kelompok tikus hiperglikemia yang diberi glibenklamid dosis 2,25 mg/kg BB. P3, P4, dan P5 adalah kelompok tikus yang diberi ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB. Data dianalisis dengan ANOVA pada signifikansi 95%. Hasil penelitian menunjukkan bahwa perlakuan ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB tidak memberikan pengaruh nyata terhadap bobot pankreas, diameter, luas, dan densitas pulau Langerhans (P>0,05). Skoring struktur pulau Langerhans berdasarkan uji Mann-Whitneymenunjukkan hasil beda nyata pada kelompok tikus yang diberi daun mimba (P≤0,05). Kesimpulan dari penelitian ini, pemberian ekstrak daun mimba dosis 400mg/kg BB menunjukkan adanya perbaikan morfologi pulau Langerhans. The clinical indicator of Diabetes mellitus was hyperglycemia. Azadirachta indica A. Juss was a plant has the potential to alternative medicine for hyperglycemia. The study was to analyze the ethanol neem leaf extract effect on histological structure of hyperglycemic rat pancreas. This study used a completely randomized design (CRD) with 24 rats were divided into 6 treatment groups and 4 replications. P0 (control) was a normal rats group were given distilled water, P1 (negative control) was a hyperglycemic rats group were given distilled water. P2 (positive control) was a hyperglycemic rats were given 2.25 mg/kg BW of glibenclamide. P3, P4, and P5 were rats were given 100, 200, and 400 mg/kg BW of ethanolic neem leaf extract.The data analyzed by ANOVA at 95% significance showed the treatment of 100, 200, and 400 mg/kg BW ethanolic neem leaf extract had no significant effect on the pancreatic weight, diameter, area, and density of Langerhans islet (P> 0.05). The score of Langerhans islet structure based on the Mann-Whitney test showed significant differences in the groups of mice given neem leaves (P≤0.05). Treatment of 400mg/Kg BW neem leaf extract showed an improvement in the morphology of the islets of Langerhans.

Effect of Different Process Parameters on the formation of Silver Nanoparticles using Crude and Modified Neem (Azadirachta indica) Leaf Extracts

In recent times, the biosynthesis of nanoparticles, which has led to significant growth in the field of nanotechnology. The use of plant extracts has become an impetus in this field as it is a simple and eco-friendly method. This study was an attempt to study different parameters in biosynthesis of silver nanoparticles using Azadirachta indica (Neem) leaf extracts. Four different process parameters such as concentrations of neem leaf extract, types of neem leaf extract, mixing ratios and the reaction time period were investigated on the formation of silver nanoparticles. Initially, the formation of silver nanoparticles was detected by the visual observation. Then, the synthesized silver nanoparticles were characterized using UV-Visible spectroscopy and scanning electron microscopy (SEM). The change of color from yellow to reddish brown color confirmed the formation of silver nanoparticles. The silver surface plasmon resonance (SPR) band obtained in the expected visible range of UV-Visible spectroscopy confirmed the synthesis of the nanoparticles. SEM images showed that silver nanoparticles are roughly spherical and of uniform particle size, and the average particle size is 100 nm. Further, the maximum absorbance of SPR band was considerably varied with different process parameters used in the present study. The UV-Visible spectra of 2.5 g/100 mL of crude neem leaf extract without any dilution showed maximum absorbance in the expected range with the mixing ratio of (Neem and AgNO3) 1:8. However, the maximum absorbance of modified neem leaf extracts (pH 10) resulted lower in value than the crude extracts in the 20 times diluted sample with the mixing ratio of 1:9. Moreover, modified extract with UV radiation exposure increased the absorbance in the expected visible range. It concludes that fine tuning of the bioprocess parameters would enhance nanoparticle synthesis.