Studies on the Nucleic Acids of the Malaria Parasite, Plasmodium Berghei (Vincke & Lips)

Changes in� the nucleic acid content of the solid residue obtained by haemolysing the blood of mice infected with Plasmodium berghei have been examined. The residue from blood in which 25 per cent. of the red blood cells were parasitized contained 20-25 times as much ribosenucleic acid (RNA) and 12 times as much desoxyribosenucleic acid (DNA) as the residue from uninfected blood.

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Bat Red Blood Cells express Nucleic Acid Sensing Receptors and bind RNA and DNA

10.1101/2022.01.13.476238 ◽

2022 ◽

Author(s):

LK Metthew Lam ◽

Jane Dobkin ◽

Kaitlyn A. Eckart ◽

Ian Gereg ◽

Andrew DiSalvo ◽

...

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Red Blood Cells ◽

Immune Function ◽

Blood Cells ◽

Toll Like Receptors ◽

Cpg Dna ◽

Human Rbcs ◽

Insight Into ◽

Rna And Dna

Red blood cells (RBCs) demonstrate immunomodulatory capabilities through the expression of nucleic acid sensors. Little is known about bat RBCs, and no studies have examined the immune function of bat erythrocytes. Here we show that bat RBCs express the nucleic acid-sensing Toll-like receptors TLR7 and TLR9 and bind the nucleic acid ligands, single-stranded RNA, and CpG DNA. Collectively, these data suggest that, like human RBCs, bat erythrocytes possess immune function and may be reservoirs for nucleic acids. These findings provide unique insight into bat immunity and may uncover potential mechanisms by which virulent pathogens in humans are concealed in bats.

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Publisher Correction: A protease cascade regulates release of the human malaria parasite Plasmodium falciparum from host red blood cells

Nature Microbiology ◽

10.1038/s41564-018-0134-6 ◽

2018 ◽

Vol 3 (4) ◽

pp. 523-523 ◽

Cited By ~ 1

Author(s):

James A. Thomas ◽

Michele S. Y. Tan ◽

Claudine Bisson ◽

Aaron Borg ◽

Trishant R. Umrekar ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Red Blood Cells ◽

Blood Cells ◽

Malaria Parasite ◽

Human Malaria Parasite ◽

Human Malaria ◽

Parasite Plasmodium ◽

Parasite Plasmodium Falciparum

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Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

Australian Journal of Agricultural Research ◽

10.1071/ar9760669 ◽

1976 ◽

Vol 27 (5) ◽

pp. 669 ◽

Cited By ~ 2

Author(s):

DA Little ◽

LJ Lambourne

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Ribonucleic Acid ◽

Acid Content ◽

Deoxyribonucleic Acid ◽

Total Content ◽

Mean Value ◽

Nucleic Acid Content ◽

Uterine Tissue ◽

Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

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Detection of short-chain carbonyl products of lipid peroxidation from malaria-parasite (Plasmodium vinckei)-infected red blood cells exposed to oxidative stress

Biochemical Journal ◽

10.1042/bj2490063 ◽

1988 ◽

Vol 249 (1) ◽

pp. 63-68 ◽

Cited By ~ 35

Author(s):

G D Buffinton ◽

N H Hunt ◽

W B Cowden ◽

I A Clark

Keyword(s):

Lipid Peroxidation ◽

Red Blood Cells ◽

Blood Cells ◽

Malaria Parasite ◽

Radical Scavenger ◽

Parasite Plasmodium ◽

Infected Rbcs ◽

Carbonyl Products ◽

Plasmodium Vinckei

Reversed-phase h.p.l.c. was used to detect 2,4-dinitrophenylhydrazine-reactive carbonyl products, which excludes malonaldehyde, in malaria-parasite (Plasmodium vinckei)-infected murine red blood cells (RBCs). A number of alkanals, 4-hydroxyalk-2-enals and alka-2,4-dienals were tentatively identified by comparison with authentic standards. The formation of 4-hydroxynon-2-enal, deca-2,4-dienal and hexanal was greater in P. vinckei-infected RBCs than in their uninfected counterparts and was increased by the presence of t-butyl hydroperoxide. Several of these aldehydes have previously been shown to be toxic to various types of cells, including P. falciparum, in vitro. The iron chelator desferrioxamine and the free-radical scavenger butylated hydroxyanisole inhibited the formation of these aldehydes. These experiments demonstrate that products of lipid peroxidation other than malonaldehyde are formed during the exposure of malaria-infected RBCs in vitro to drugs that generate reactive oxygen species and have anti-parasitic activity. The formation of products of this type during the natural course of malaria infection may have implications for the mechanisms underlying intra-RBC parasite death and the tissue damage associated with the disease.

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Isolation of skeleton-associated knobs from human red blood cells infected with malaria parasite Plasmodium falciparum

Molecular and Biochemical Parasitology ◽

10.1016/0166-6851(92)90062-o ◽

1992 ◽

Vol 52 (2) ◽

pp. 283-287 ◽

Cited By ~ 11

Author(s):

Athar H. Chishti ◽

Khurshid I. Andrabi ◽

Laura H. Derick ◽

Jiri Palek ◽

Shih-Chun Liu

Keyword(s):

Plasmodium Falciparum ◽

Red Blood Cells ◽

Blood Cells ◽

Malaria Parasite ◽

Human Red Blood Cells ◽

Parasite Plasmodium ◽

Parasite Plasmodium Falciparum

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Loading of Human Red Blood Cells with DNA and RNA

Zeitschrift für Naturforschung C ◽

10.1515/znc-1976-3-410 ◽

1976 ◽

Vol 31 (3-4) ◽

pp. 149-151 ◽

Cited By ~ 5

Author(s):

Dieter Auer ◽

Gerhard Brandner

Keyword(s):

Nucleic Acid ◽

Nucleic Acids ◽

Red Blood Cells ◽

Blood Cells ◽

Human Erythrocytes ◽

Viral Dna ◽

External Concentration ◽

Dna And Rna ◽

Human Red Blood Cells ◽

Hank’S Solution

Abstract Human erythrocytes were suspended in Hank’s solution containing mammalian or viral DNA or RNA. After dialysis at 0 °C first against water and subsequently against Hank’s solution, and a further incubation at 37 °C , the erythrocytes were found to be loaded with the nucleic acids. The nucleic acid trapped in the erythrocytes exhibited up to 35 per cent of the external concentration.

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