Pollen competition does not affect the success of self-pollination in Eucalyptus globulus (Myrtaceae)

2003 ◽  
Vol 51 (2) ◽  
pp. 189 ◽  
Author(s):  
L. M. Pound ◽  
B. Patterson ◽  
M. A. B. Wallwork ◽  
B. M. Potts ◽  
M. Sedgley
Keyword(s):  
Eucalyptus Globulus ◽  
Pollen Tubes ◽  
Pollen Competition ◽  
Isozyme Analysis ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Self Pollination ◽  
Number Of Seeds

This study investigated whether pollen competition favours cross- over self-pollination in Eucalyptus globulus Labill. ssp. globulus. Controlled pollinations with self-pollen, cross-pollen and a mixture of self- and cross-pollen were conducted on three partially self-incompatible trees. The paternity of individual seeds resulting from mixed pollination was determined by isozyme analysis. No evidence for pollen competition was found. Instead, seed paternity reflected the level of self-incompatibility of each tree as determined by separate self- and cross-pollinations. Furthermore, the number of seeds set per capsule following mixed pollination was significantly less than that following cross-pollination in the two least self-compatible trees. These results suggest that both self- and cross-pollen tubes reach ovules following mixed pollination and that a late-acting self-incompatibility mechanism operates to abort a certain proportion of self-penetrated ovules.

scholarly journals Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 432
Author(s):  
Yaling Chen ◽  
Benchang Hu ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
Jiankun Xie
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Dendrobium Officinale ◽  
Self Incompatibility ◽  
Related Gene ◽  
Cytological Observation ◽  
Lectin Receptor ◽  
Cross Pollination ◽  
Self Pollination ◽  
Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

Late-acting self-incompatibility in tea plant (Camellia sinensis)

Biologia ◽  
2012 ◽  
Vol 67 (2) ◽  
Author(s):  
Xuan Chen ◽  
Shan Hao ◽  
Li Wang ◽  
Wanping Fang ◽  
Yuhua Wang ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Camellia Sinensis ◽  
Pollen Tubes ◽  
Tea Plant ◽  
The Self ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Self Pollination ◽  
Hand Pollination ◽  
Pollen Tube Elongation

AbstractThe self-incompatibility of tea plant (Camellia sinensis (L.) O. Kuntze) was studied with the methods of aniline blue fluorescence assay and paraffin sections. The characteristics of pollen tube elongation after hand pollination was analyzed in 4 tea cultivars, including ‘Keemenzhong’, ‘Longjing-changye’, ‘Fuding-dabaicha’ and ‘Yabukita’, under self-pollination and cross-pollination, respectively. Although there were some difference among cultivars, pollen tubes elongated through the style and reach the ovary successfully at 48 h after pollination for both cross- and self-pollen tubes in all the four cultivars of tea. Pollen tubes entered into the ovule micropyles, however, only for cross-pollination, but not for self-pollination. Pollen tubes of selfing plants, failed in fertilizing, seemed have some difficulties to enter the ovule. All of which indicated that the self-incompatibility of tea plant is a late-acting self-incompatibility system (LSI) or an ovarian sterility (OS), in which the self incompatibility was due to none self pollen tube penetrating into the ovule and no fertilization.

scholarly journals Cultivar and Developmental Differences in S-protein Concentration and Self-incompatibility in the Japanese Pear

HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 917-920 ◽  
Author(s):  
Shao-Ling Zhang ◽  
Shin Hiratsuka
Keyword(s):  
Pollen Tubes ◽  
Tube Growth ◽  
Cultivar Differences ◽  
Japanese Pear ◽  
Pyrus Pyrifolia ◽  
Self Incompatibility ◽  
S Protein ◽  
Self Pollination ◽  
Rate Of Increase ◽  
S Proteins

Cultivars of the Japanese pear [Pyrus pyrifolia (Burm.) Nakai] have variable degrees of self-incompatibility (SI) and can be classified into at least three groups: strong, intermediate, or weak SI; as shown by the extent of self-pollen tube growth in the style, and the percentage of fruit set following self-pollination. Following self-pollination, the elongation of pollen tubes in the detached styles of `Kosui' and `Kikusui' became increasingly suppressed from 4 days before anthesis (–4 DAA) to 2 days after anthesis (2 DAA). Tube growth of `Kosui' was more suppressed than that of `Kikusui' during this period. In `Osa-Nijisseiki', however, the rate of tube growth did not vary with stage of stylar development, from –8 to 2 DAA. Pollen tubes elongated much better after cross-pollination than after self-pollination at all stages tested, and the extent of the elongation increased as the styles matured. The concentration of total S-protein (sum of two S-proteins per buffer-soluble protein) increased with stylar development, but the rate of increase varied with the cultivar. The rate was significantly greater in the strongly self-incompatible `Kosui' than in the moderately self-incompatible `Kikusui', and was slowest in the weakly self-incompatible `Osa-Nijisseiki' at all developmental stages. During stylar maturation, the concentration of S4-protein, which is common in all cultivars, was highest in `Kosui', followed by `Kikusui' and `Osa-Nijisseiki'. Thus, the cultivar differences in SI expression in the Japanese pear are determined about –4 DAA and appear to be regulated, in part, by the concentration of S-proteins produced in the style.

scholarly journals Reproductive Success after Self-pollination and Cross-pollination of Olive Cultivars in Croatia

HortScience ◽  
2011 ◽  
Vol 46 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Gabriela Vuletin Selak ◽  
Slavko Perica ◽  
Smiljana Goreta Ban ◽  
Mira Radunic ◽  
Milan Poljak
Keyword(s):  
Reproductive Behavior ◽  
Fruit Set ◽  
Positive Response ◽  
Flowering Period ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Self Pollination ◽  
Cross Compatibility ◽  
Olive Cultivars ◽  
Simultaneous Flowering

Olive orchard productivity largely depends on the choice of planted cultivars and their pollination needs. Orchard designs in Croatia are changing because a number of valuable foreign olive cultivars, mostly Italian, have been introduced in this region in the last 30 years. The compatibility relationships of introduced cultivars with autochthonous cultivars are unknown. With the objective of studying reproductive behavior of the most important Croatian cultivars (Drobnica, Lastovka, Levantinka, and Oblica) and their cross-pollination to recently introduced Italian cultivars Leccino and Pendolino, initial and final fruit set in self-pollination versus cross-pollination and free pollination were compared during three flowering seasons. Experiments were conducted in three different orchards (Kastela, Mravince, and Brac) to identify the effect of the environment on reproductive behavior of olive cultivars. The differences of fruit set in five olive cultivars after tested pollination treatments appeared at the time of initial fruit set. Increased final fruit set under cross-pollinations was observed when compared with self-pollination for all olive cultivars in all experimental orchards. In the Mravince orchard, a positive response to cross-pollination was consistent, and fruit set increased under cross-pollination in all cultivars and years with the exception of ‘Levantinka’ in which no significant differences were noticed between self-pollination treatment and cross-pollination treatments in 2005. Variable self-fertility behavior from season to season was found for tested cultivars. A self-incompatibility index (ISI) higher than 0.1 was recorded for ‘Levantinka’ in all experimental years and, therefore, classified it as a partially self-incompatible cultivar. Self-incompatibility response was observed for ‘Lastovka’. The positive response to cross-pollination over self-pollination only in some experimental years classified ‘Drobnica’, ‘Leccino’, and ‘Oblica’ as partially self-incompatible. Results obtained from this study indicated that pollination efficiency is strictly combination-specific. The Italian cultivar, Leccino, was a successful pollen acceptor and pollenizer of most Croatian cultivars. Reciprocal high success in cross-pollination was recorded for ‘Levantinka’ and ‘Oblica’. In the Mravince orchard, ‘Lastovka’, ‘Leccino’, and ‘Oblica’ were efficient pollenizers of ‘Levantinka’ where the simultaneous flowering period was in accordance with their cross-compatibility. ‘Levantinka’ was a good pollenizer for ‘Lastovka’ in the Mravince orchard, and both cultivars entered into the flowering period earlier than other studied cultivars, which was not the case in the other two orchards. The variations in flowering timing among orchards were a consequence of differences in environmental conditions. According to the high fruit sets recorded in ‘Oblica’ after pollination with ‘Leccino’ or ‘Levantinka’, an increase in tree productivity of the acceptor cultivar is expected in the presence of selected pollenizers in all olive-growing regions.

scholarly journals Transcriptomic Analysis Reveals a Comprehensive Calcium- and Phytohormone-Dominated Signaling Response in Leymus chinensis Self-Incompatibility

2019 ◽  
Vol 20 (9) ◽  
pp. 2356 ◽  
Author(s):  
Shuangyan Chen ◽  
Junting Jia ◽  
Liqin Cheng ◽  
Pincang Zhao ◽  
Dongmei Qi ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Time Course ◽  
Molecular Mechanisms ◽  
Tube Growth ◽  
Leymus Chinensis ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Grass Family ◽  
Self Pollination

Sheepgrass (Leymus chinensis (Trin.) Tzvel.) is an economically and ecologically important forage in the grass family. Self-incompatibility (SI) limits its seed production due to the low seed-setting rate after self-pollination. However, investigations into the molecular mechanisms of sheepgrass SI are lacking. Therefore, microscopic observation of pollen germination and pollen tube growth, as well as transcriptomic analyses of pistils after self- and cross-pollination, were performed. The results indicated that pollen tube growth was rapidly inhibited from 10 to 30 min after self-pollination and subsequently stopped but preceded normally after cross-pollination. Time course comparative transcriptomics revealed different transcriptome dynamics between self- and cross-pollination. A pool of SI-related signaling genes and pathways was generated, including genes related to calcium (Ca2+) signaling, protein phosphorylation, plant hormone, reactive oxygen species (ROS), nitric oxide (NO), cytoskeleton, and programmed cell death (PCD). A putative SI response molecular model in sheepgrass was presented. The model shows that SI may trigger a comprehensive calcium- and phytohormone-dominated signaling cascade and activate PCD, which may explain the rapid inhibition of self-pollen tube growth as observed by cytological analyses. These results provided new insight into the molecular mechanisms of sheepgrass (grass family) SI.

Outcrossing in interspecific hybrids between Eucalyptus spathulata and E. platypus

2005 ◽  
Vol 53 (4) ◽  
pp. 347 ◽  
Author(s):  
M. A. B. Wallwork ◽  
M. Sedgley
Keyword(s):  
Interspecific Hybrids ◽  
Female Parent ◽  
Pollen Tubes ◽  
The Other ◽  
Parent Species ◽  
Cross Pollination ◽  
Seed Maturity ◽  
Self Pollination ◽  
Ovule Penetration ◽  
Zygotic Control

Outcrossing was investigated in interspecific hybrids between self-fertile Eucalyptus platypus Blakely and partially self-sterile E. spathulata Hook., which shows both pre- and post-zygotic timing. Four hybrid trees were used for the study, two with E. spathulata and two with E. platypus as female parent. Each hybrid had a similar number of locules to each other and to the E. platypus parent, and an intermediate number of ovules per flower compared with the parent species. Controlled hand-pollinations were carried out, in which both self- and cross-pollen from the other hybrid tree with the same female parent species was applied to flowers on each of the four trees, and observations were made 10days, 4weeks and 8weeks after pollination and at seed maturity. In all hybrids, mean seeds per capsule was consistently higher following cross-pollination than following self-pollination. All hybrids showed a reduction in pollen tube number between the top and base of the style when examined by fluorescence microscopy. One tree had significantly fewer cross- than self-pollen tubes at the base of the style, but a similar number of ovules was penetrated by pollen tubes following both treatments. In the other three, there was no difference between cross- and self-pollination in pollen tubes in the style. In three of the four trees there was no difference in ovule penetration following self- or cross-pollination, but in the other, more crossed than selfed ovules were penetrated. Light-microscopy observation of ovules indicated that ovule abortion following fertilisation accounted for the reduced numbers of seeds following self-pollination and to a lesser extent following cross-pollination. All four hybrid trees, irrespective of female parent, were partially self-sterile and resembled the partially self-sterile E. spathulata rather than the self-fertile E. platypus. While the timing of outcrossing control of E. spathulata was both pre- and post-zygotic, only one hybrid was similar, with the other three showing post-zygotic control.

Breeding System and Pollination Levels of Banksia ericifolia

1991 ◽  
Vol 39 (4) ◽  
pp. 365 ◽  
Author(s):  
RL Goldingay ◽  
SM Schibeci ◽  
BA Walker
Keyword(s):  
Breeding System ◽  
Pollen Tubes ◽  
Fruit Production ◽  
The Self ◽  
Open Pollination ◽  
Flower Number ◽  
Cross Pollination ◽  
Self Pollination ◽  
Full Complement ◽  
Banksia Ericifolia

Experiments were carefully designed to determine the breeding system of Banksia ericifolia L.f. An equivalent percentage of flowers (78%) contained pollen tubes following self-pollination and open-pollination while a significantly smaller percentage of flowers in an autogamy treatment (44%) and cross-pollination treatment (55%) contained pollen tubes. Significantly more of the inflorescences in the open-pollination (60%) and cross-pollination (33%) treatments produced fruit compared with those in the self-pollination (11%) and autogamy treatments (13%). We suggest that B. ericifolia is largely self-incompatible because fruit production did not reflect pollen tube abundances. The influence of pollination levels on fruit production was determined by reducing the number of flowers on an inflorescence to 100 (i.e. 10% of original) or fewer and hand-pollinating these with cross pollen. There was no difference in fruit production between inflorescences with reduced flower number and open-pollinated inflorescences which had their full complement of flowers. Thus, the ability of an inflorescence to produce fruit appears more likely to be determined by the type of pollen received (cross versus self) rather than by the number of pollinated flowers it contains.

scholarly journals Reciprocal intercompatibility between Byrsonima Rich. exKunth species (Malpighiaceae), in an urban ecotonal fragment of Semi-Deciduous Seasonal Forest and Cerrado

2021 ◽  
pp. 211-221
Author(s):  
Frederic Mendes Hughes
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Prezygotic Isolation ◽  
Stigmatic Surface ◽  
Cross Pollination ◽  
Self Pollination ◽  
Isolation Mechanisms ◽  
Hypothesis Evaluation

Byrsonima species present floral monomorphism and sharing visitor/pollinator guild. These traits suggest the hypothesis evaluation of reciprocal inter-incompatibility between two syntopic Byrsonima species: B. pachyphylla and B. crassifolia. Pollen tube growth with similar behavior was observed in the stigmatic surface, pistil canal, ovary and micropylar channel to both species. In addition, partial self-incompatibility in self-pollination with greater fruiting in autogamy was observed. Cross-pollination and self-pollination coexist, and reciprocal intercompatibility occurs. Prezygotic isolation mechanisms are unlikely by the absence of abnormal pollen tubes, higher fruiting production and absence of hybrids in the study site. Las especies de Byrsonima presentan monomorfismo floral y comparten gremio de visitantes/polinizadores. Estos rasgos sugieren la evaluación de hipótesis de interincompatibilidad recíproca entre dos especies sintópicas de Byrsonima: B. pachyphylla y B. crassifolia. Se observó el crecimiento del tubo polínico con comportamiento similar en la superficie estigmática, canal del pistilo, ovario y canal micropilar de ambas especies. Además, se describió la autoincompatibilidad parcial en la autopolinización con mayor fructificación en la autogamia. La polinización cruzada y la autopolinización coexisten y se produce una intercompatibilidad recíproca. Los mecanismos de aislamiento precigóticos son improbables por la ausencia de tubos polínicos anormales, producción de frutos y ausencia de híbridos en la área de estudio.

scholarly journals Comparison of different classical and molecular methods for identifying self-incompatibility in two olive cultivars

2019 ◽  
Vol 17 (3) ◽  
pp. e0804 ◽  
Author(s):  
Elham Aslmoshtaghi ◽  
Ali Reza Shahsavar ◽  
Majid Talebi ◽  
Aziz Dazeh
Keyword(s):  
Pollen Tube ◽  
Molecular Analysis ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Research Station ◽  
Self Incompatibility ◽  
Controlled Pollination ◽  
Cross Pollination ◽  
Self Pollination ◽  
Olive Cultivars

Aim of study: To determine compatibility relationships and select suitable pollinizers for two olive (Olea europaea L.) cultivars (‘Amygdalolia’ and ‘Konservalia’).Area of study: The Kazeroun Olive Research Station, Fars, Iran.Material and methods: Emasculated flowers from ‘Amygdalolia’ and ‘Konservalia’ cultivars were treated with self-pollination, open-pollination, and cross-pollination with pollen from cultivars such as ‘Dacal’, ‘Amygdalolia’,‘Konservalia’, ‘Koroniki’, and ‘Manzanilla’. Controlled pollination, pollen tube growth, and molecular analysis were employed.Main results: Controlled pollination, pollen tube growth, and molecular analysis showed that cross-pollination was beneficial for ‘Amygdalolia’ compared to self-pollination. The results showed that this cultivar is self-incompatible, and its best pollinator is the ‘Dacal’ cultivar. Experiment results indicated that ‘Konservalia’ behaves as a self-compatible cultivar. The highest fruit percentage and higher pollen tube growth rates were found in self-pollination treatments. Molecular attempts to isolate candidates for sporophytic self-incompatibility (SSI) led the researchers to analyze the expression of SRK and SLG genes.Research highlights: The results indicated an antagonist transcriptional expression pattern in the flowers of ‘Amygdalolia’, classified as a self-incompatible cultivar, and ‘Konservalia’, classified as a self-compatible cultivar, for the SRK and SLG genes.

scholarly journals Possibilities of use of self-pollinated lines for creating of synthetic hemp populations

2018 ◽  
pp. 38-46
Author(s):  
Serhiy Mishchenko ◽  
Iryna Layko
Keyword(s):  
Use Of Self ◽  
Seed Productivity ◽  
Synthetic Variety ◽  
Cross Pollination ◽  
Breeding Material ◽  
Self Pollination ◽  
The Future ◽  
Spatial Isolation ◽  
Number Of Seeds

It has been proved that it is possible to create a new breeding material or increase the productivity of an existing one by obtaining stable self-pollinated lines on its basis, followed by their free pollination and propagation according to a specially developed technique (by the type of creation of a synthetic variety). Moreover, at the initial stages of breeding, it is important to select self-pollinated lines according to the set of desired characteristics, at the final stages of closely related breeding, leave only those lines that form the smallest number of seeds as a result of self-pollination, and according to the nursery, estimates in the absence of spatial isolation and free pollination with pollen of various origin characterized by high seed productivity. This technique allows you to select genotypes that are less capable of self-pollination, and more capable of cross-pollination, which in the future will increase the viability and productivity of generation.

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