scholarly journals Possibilities of use of self-pollinated lines for creating of synthetic hemp populations

2018 ◽  
pp. 38-46
Author(s):  
Serhiy Mishchenko ◽  
Iryna Layko
Keyword(s):  
Use Of Self ◽  
Seed Productivity ◽  
Synthetic Variety ◽  
Cross Pollination ◽  
Breeding Material ◽  
Self Pollination ◽  
The Future ◽  
Spatial Isolation ◽  
Number Of Seeds

It has been proved that it is possible to create a new breeding material or increase the productivity of an existing one by obtaining stable self-pollinated lines on its basis, followed by their free pollination and propagation according to a specially developed technique (by the type of creation of a synthetic variety). Moreover, at the initial stages of breeding, it is important to select self-pollinated lines according to the set of desired characteristics, at the final stages of closely related breeding, leave only those lines that form the smallest number of seeds as a result of self-pollination, and according to the nursery, estimates in the absence of spatial isolation and free pollination with pollen of various origin characterized by high seed productivity. This technique allows you to select genotypes that are less capable of self-pollination, and more capable of cross-pollination, which in the future will increase the viability and productivity of generation.

scholarly journals The formation of seed productivity of a peas collection of different morphotypes in the Middle Volga region (PoVolzhie)

2020 ◽  
pp. 32-38
Author(s):  
A. I. Katyuk
Keyword(s):  
Seed Weight ◽  
Geographical Origin ◽  
Current Paper ◽  
Volga Region ◽  
Seed Productivity ◽  
Middle Volga Region ◽  
High Productivity ◽  
Breeding Material ◽  
Middle Volga ◽  
Number Of Seeds

The purpose of the current paper was to study the features of the formation of productivity components and their correlation in the peas varieties of different morphotypes to develop new breeding material with high productivity. The study was carried out in the conditions of the Middle Volga region (PoVolzhie) in 2005, 2008-2011 and 2018-2019 according to generally accepted methods. The objects of the study were the pea varieties of various ecological and geographical origin of domestic and foreign selection. There were studied from 50 to 194 varieties in different years. High seed productivity was identified in the mustachioed determinant group (3.5 g) and the chameleon group (4.0 g). The seed weight in the peas varieties belonging to these groups was 0.5 and 1.0 g per plant or on 16-30% higher than that of the traditional leafy indeterminate group of varieties, respectively. There has been established a positive correlation between seed weight per plant and number of seeds per plant (r = 0.742-0.887), number of beans per plant (r = 0.736-0.814) and seed weight per fertile node (r = 0.716-0.851). There has been shown that the variability of pea productivity was largely stipulated by number of seeds per plant in the favorable climatic years and by seed weight per fertile node in the arid years. In various morphological groups there have been identified the sources of high seed productivity, maximally adapted to the conditions of the region. The best sources ‘Ras tip', ‘Az26', ‘Az96637', ‘Flagman 9', ‘Az931776', the determinant ‘VSKhI', ‘Harvus', ‘Faraon', ‘Flagman 7', ‘Orel', ‘Kuibyshevsky', ‘Voronezhsky', ‘B3390/11') will be included in the breeding process.

Pollen competition does not affect the success of self-pollination in Eucalyptus globulus (Myrtaceae)

2003 ◽  
Vol 51 (2) ◽  
pp. 189 ◽  
Author(s):  
L. M. Pound ◽  
B. Patterson ◽  
M. A. B. Wallwork ◽  
B. M. Potts ◽  
M. Sedgley
Keyword(s):  
Eucalyptus Globulus ◽  
Pollen Tubes ◽  
Pollen Competition ◽  
Isozyme Analysis ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Self Pollination ◽  
Number Of Seeds

This study investigated whether pollen competition favours cross- over self-pollination in Eucalyptus globulus Labill. ssp. globulus. Controlled pollinations with self-pollen, cross-pollen and a mixture of self- and cross-pollen were conducted on three partially self-incompatible trees. The paternity of individual seeds resulting from mixed pollination was determined by isozyme analysis. No evidence for pollen competition was found. Instead, seed paternity reflected the level of self-incompatibility of each tree as determined by separate self- and cross-pollinations. Furthermore, the number of seeds set per capsule following mixed pollination was significantly less than that following cross-pollination in the two least self-compatible trees. These results suggest that both self- and cross-pollen tubes reach ovules following mixed pollination and that a late-acting self-incompatibility mechanism operates to abort a certain proportion of self-penetrated ovules.

scholarly journals Pollinator Contribution to the Production of Cowpea in the Amazon

HortScience ◽  
1998 ◽  
Vol 33 (7) ◽  
pp. 1157-1159 ◽  
Author(s):  
Carlos G. Vaz ◽  
Domingos de Oliveira ◽  
Orlando S. Ohashi
Keyword(s):  
Experimental Design ◽  
Vigna Unguiculata ◽  
Fruit Set ◽  
Open Pollination ◽  
Cross Pollination ◽  
Self Pollination ◽  
Complementary Role ◽  
Pollinating Insects ◽  
The Mean ◽  
Number Of Seeds

Cowpea, Vigna unguiculata (L.) Walp., is a very important legume in the diet of the population of the Amazon. Although it is autogamous, this species has a cross-pollination rate of ≈10%. Over several years, the mean productivity of cowpea has declined. We suggest that this is linked to a decrease in or an absence of pollinating insects in the fields. The objective of this study is to ascertain the pollinator contribution to cowpea production, as well as to determine the pollination type of the `BR3-Tracuateua' cultivar. In an experimental design, four treatments were compared: no pollination, with flowers in cages to prevent insect visits; open-pollination, with flowers exposed to all visiting insects; self-pollination, with flowers pollinated with their own pollen; and cross-pollination, with emasculated flowers being pollinated manually with pollen from another plant. We observed higher fruit set in the presence of pollinators (83%) than in their absence (77%, caged flowers). However, cross-pollination reduced both the number of seeds per pod and fruit set relative to self-pollination. This result suggests that pollinators have a complementary role in the yield of cowpea, by creating a mixed pollination system where self-pollination dominates.

scholarly journals Cytological Observation and Transcriptome Comparative Analysis of Self-Pollination and Cross-Pollination in Dendrobium Officinale

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 432
Author(s):  
Yaling Chen ◽  
Benchang Hu ◽  
Fantao Zhang ◽  
Xiangdong Luo ◽  
Jiankun Xie
Keyword(s):  
Pollen Tubes ◽  
The Self ◽  
Dendrobium Officinale ◽  
Self Incompatibility ◽  
Related Gene ◽  
Cytological Observation ◽  
Lectin Receptor ◽  
Cross Pollination ◽  
Self Pollination ◽  
Medicinal Value

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

scholarly journals Time of flowering and fertilisation of quince varieties

1999 ◽  
Vol 5 (1-2) ◽  
Author(s):  
T. Szabó ◽  
J. Nyéki ◽  
M. Soltész ◽  
Z. Szabó ◽  
T. Tóth
Keyword(s):  
Fruit Set ◽  
Fruit Size ◽  
Late Flowering ◽  
Cross Pollination ◽  
Self Pollination ◽  
The Individual ◽  
Contradictory Data

Literature dealing with flowering and fertilisation of quince is scarce. Most controversial and scanty are informations on observations of self- and cross-pollination. According to our observations, differences in blooming time are few (2-3) days only, thus flowering of most varieties is synchronous. The varieties observed are grouped as early, intermediate and late flowering ones. Self fertility of the individual varieties, however, was not assessed unequivocally, therefore it is recommended, by safety reasons, to consider quince actually as a whole to be auto-incompatible. Artificial self-pollination (or rather geitonogamy) as well as cross pollination with other varieties increased substantially fruit set if compared with the results of natural self-pollination (autogamy). According to the fruit set of their open pollinated flowers, varieties have been classified according to fertility as low (below 10 %), medium (between 10 and 20 %) and high (more than 20 %). Cross fertility of varieties is highly variable depending on combination and on season. Contradictory data are probably due to the sensitivity of quince to conditions of search. Better fruit set was coincident with higher number of stout seeds per fruit. Well developed seeds are definitely a prerequisite of larger fruit size.  

scholarly journals Creation of a new breeding material for creeping clover (Trifolium repens L.) hay-pasture type using the hybridization method

2021 ◽  
Vol 901 (1) ◽  
pp. 012046
Author(s):  
A A Ivanova
Keyword(s):  
Trifolium Repens ◽  
Hydrocyanic Acid ◽  
First Year ◽  
Hybridization Method ◽  
Seed Productivity ◽  
Early Maturity ◽  
Breeding Material ◽  
Reciprocal Crossing ◽  
Trifolium Repens L ◽  
First Year Of Life

Abstract The studies were carried out in the Moscow region in 2019-2021. In order to create a new breeding material for creeping hay-pasture clover, samples of the Lodian type (giganteum variety), characterized by high peduncles and longer leaf petioles, and a low content of hydrocyanic acid (0.3 mg%), were selected as parental forms. Reciprocal crossing was carried out. Hybrids F1 and F2 in the first year of life were assessed by the main economic and biological characteristics: the length of the peduncles, the number of inflorescences on the plant, fodder and seed productivity, early maturity, winter hardiness and other indicators. Based on the assessment results, the best hybrids and individual biotypes were identified.

scholarly journals SEED PRODUCTIVITY AND DEVELOPMENT OF PLANTLETS POPULUS × JRTYSCHENSIS CH. Y. YANG

2019 ◽  
pp. 51-57
Author(s):  
B. V. Proshkin ◽  
A. V. Klimov
Keyword(s):  
Filter Paper ◽  
Fruit Set ◽  
Control Group ◽  
Natural Hybrid ◽  
Seed Productivity ◽  
Model Trees ◽  
Fruit Number ◽  
Black Poplar ◽  
Petri Dishes ◽  
Number Of Seeds

The research explores the seed productivity and plantlets growth in the free pollination of the natural hybrid taxon P. × jrtyschensis. Fruits of P. × jrtyschensis were selected from four plants that grow in the collection ofResearchCenter“EducationalBotanical Garden” ofKemerovoStateUniversity. Four P. nigra model trees, randomly selected from theTomRiverfloodplain population, were applied as a control group. The authors used 30 fruit-bearing amentumsfrom each model. The researchers measured set of fruit (capsule); number of ovules per fruit; number of seeds per fruit; set of seeds.. Laboratory germination was determined by sowing Petri dishes on wet filter paper. The authors found out sowing germination by sowing 100 seeds in a box with soil and drainage. The energy of germination was determined on the second day while germination - on the fifth day. P. × jrtyschensis is characterized by a lower level of seed productivity (15-30%) compared to P. nigra. In terms of laboratory germination of seeds, the descendants of hybrids surpassed many P. nigra models, but their soil germination was 20-30% lower than that of black poplar. The observed variability in reproductive indices of both P. × jrtyschensis and P. nigra is mainly caused by specific features of their genotypes. Plantlets being developed, the authors observed no significant differences among the descendants of P. nigra and hybrids. The researchers highlighted plantlets that can stop growing and even more abnormal plants with one, three or four seeds in P. × jrtyschensis. This may be caused by underdevelopment of hypocotyl or germ root. The authors observed breaches in development of P. nigra just once. They outline high plantlets destruction when sowing hybrids on the first day after germination The share of destructed plants within a month (from the beginning of the experiment) reaches 66,0 %, and in P. nigra it does not exceed 40,0 %.

The mating system of Acrotriche serrulata (Ericaceae)

2010 ◽  
Vol 58 (2) ◽  
pp. 124 ◽  
Author(s):  
Melanie Schneemilch ◽  
Emma Steggles
Keyword(s):  
Mating System ◽  
Pollen Viability ◽  
Seed Viability ◽  
Corolla Tube ◽  
Morphological Features ◽  
Stigma Receptivity ◽  
Pollination Experiments ◽  
Controlled Pollination ◽  
Cross Pollination ◽  
Self Pollination

Controlled pollination experiments were conducted on Acrotriche serrulata R.Br. (Ericaceae) to determine the mating system for this species. Pollen viability and stigma receptivity were investigated to ensure effective timing and sourcing of material for maximum fertilisation. Stigmas were found to be receptive in mature buds, with receptivity maintained while nectar remained within the corolla tube. Germination testing showed that pollen from within mature buds was viable. The mating system of A. serrulata was found to be predominantly outcrossing, with 49.8% of cross-pollinated flowers developing to fruit, compared with 8.7% of self-pollinated flowers. Seed viability did not differ significantly between fruits produced by cross-pollination and those produced by self-pollination, although this may be attributable to low sample sizes available for self-pollinated fruits. These results in combination with morphological features of the flower suggest that a pollinator is required for this species.

The response to various pollination treatments in inbred lines of horse and tick beans (Vicia faba L.)

1974 ◽  
Vol 82 (3) ◽  
pp. 531-534 ◽  
Author(s):  
Gillian Toynbee-Clarke
Keyword(s):  
Plant Breeding ◽  
Vicia Faba ◽  
Inbred Lines ◽  
Cross Pollination ◽  
Fifth Generation ◽  
Mature Flower ◽  
Significant Difference ◽  
Vicia Faba L ◽  
Flower Stage ◽  
Number Of Seeds

SUMMARYFourth and fifth generation inbred lines of horse and tick spring beans were used for pollination studies.Treatments included tripping; emasculation followed by self- or cross-pollination at both the bud and mature flower stage; self-pollen rubbed on to the stigma; and the flower truss left undisturbed. Treatment means showed that there was no significant difference between leaving the flower truss undisturbed and tripping, but all other treatments differed significantly (P = 0·01) from these two. There was no difference in number of seeds set per flower between self- or cross-pollination following emasculation.Tick inbred lines set more seeds per flower over all treatments than horse inbreds. Differences were seen between tick and horse inbreds when the flower truss was left undisturbed; some tick inbreds set seed, but no seed was set on the horse inbreds. An association between seed size and autofertility is suggested.Results from the horse inbreds were in agreement with previous pollination studies on inbred lines of winter beans. The implications of these studies to plant breeding are discussed.

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