Factors affecting vegetative growth and the production of perithecia in culture by Ophiobolus graminis. I. Variations in media and age of mycelium

1970 ◽  
Vol 18 (1) ◽  
pp. 1 ◽  
Author(s):  
G Weste
Keyword(s):  
Carbon Concentration ◽  
Vegetative Growth ◽  
Solid Medium ◽  
Nitrogen Concentration ◽  
Restricted Range ◽  
Factors Affecting ◽  
Carbon And Nitrogen ◽  
Nitrogen Concentrations ◽  
High Concentrations ◽  
Carbon Concentrations

Perithecia were readily produced in culture on a suitable solid medium under certain conditions of light and temperature, once vegetative growth was established. Investigations into the carbon and nitrogen requirements for both vegetative growth and fruiting showed that, whereas vegetative growth increased with increasing carbon supplies up to10%, reproduction occurred only within a restricted range of carbon and nitrogen concentrations. No perithecia were produced on a starvation medium. Factors involved in fruiting included concentration of nutrients and the balance between them; both were important. A suitable fruiting medium required a minimum carbon concentration of 3000 p.p.m., supplied as 0.75 % glucose or fructose, and an optimum carbon concentration of 6000 p.p.m. supplied as 1.5 % glucose or fructose. The maximum number of perithecia was produced on a medium containing 1% glucose and 0.2 % asparagine (400 p.p.m. nitrogen), which had a carbonlnitrogen ratio of 11.8. A higher than optimum nitrogen concentration was partially offset by increasing the carbon concentration, that is by keeping the carbonlnitrogen ratio approximately constant. The actual concentrations inhibiting and promoting fruiting of the fungus were influenced by the balance between carbon and nitrogen supplies. High concentrations of carbon and nitrogen increased vegetative growth but decreased the number of perithecia. There was no sudden inhibition of perithecia with increased carbon concentrations, but at 10% glucose (40,000 p.p.m. carbon) vegetative growth and pigmentation were maximal but few or no perithecia developed. No evidence was obtained that perithecial production was influenced by ageing of the mycelium, the presence of staling factors, or exhaustion of food supplies.

scholarly journals Mycelial biomass cultivation of Lentinus crinitus

2020 ◽  
Vol 36 (6) ◽  
Author(s):  
Itaruã Machri Colla ◽  
Olavo Bilac Quaresma de Oliveira Filho ◽  
Janyeli Dorini Silva de Freitas ◽  
Míria Benetati Delgado Bertéli ◽  
Giani Andrea Linde ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Nitrogen Concentration ◽  
Fungal Growth ◽  
Malt Extract ◽  
Biomass Growth ◽  
Mycelial Biomass ◽  
Protein Nitrogen ◽  
Temperature Growth ◽  
Nitrogen Concentrations ◽  
High Concentrations

Lentinus crinitus is a medicinal basidiomycete, little studied regarding the basic cultivation conditions, which is used in bioremediation and consumed by native Indians from the Brazilian Amazon. Also, it produces a fungal secondary metabolite panepoxydone that has been described as an essential regulator of the inflammatory and immune response. This study aimed to evaluate basic conditions of temperature, pH, and nitrogen concentration and source in the cultivation of L. crinitus mycelial biomass. In order to evaluate fungal growth temperature, 2% malt extract agar (MEA) medium, pH 5.5, was utilized from 19 to 40 °C. For pH, MEA had pH adjusted from 2 to 11 and cultivated at 28 °C. Urea or soybean meal was added to MEA to obtain final concentration from 0.5 and 16 g/L of nitrogen, pH of 5.5, cultivated at 28 °C. The best temperature growth varies from 31 to 34 ºC and the optimal one is 32.7º C, and the best pH ranges from 4.5 to 6.5 and the optimal one is 6.1. Protein or non-protein nitrogen concentration is inversely proportional to the mycelial biomass growth. Nitrogen concentrations of 2.0 g/L soybean meal and urea inhibit mycelial biomass growth in 11% and 12%, respectively, but high concentrations of 16.0 g/L nitrogen inhibit the growth in 46% and 95%, respectively. The fungus is robust and grows under extreme conditions of temperature and pH, but smaller adaptation with increasing nitrogen concentrations in the cultivation medium, mainly non-protein nitrogen.

Bark degradation by Aspergillus fumigatus. Growth studies

1974 ◽  
Vol 20 (3) ◽  
pp. 283-298 ◽  
Author(s):  
U. Marie Nordström
Keyword(s):  
Energy Source ◽  
Aspergillus Fumigatus ◽  
Submerged Culture ◽  
Nitrogen Concentration ◽  
Fungal Growth ◽  
Solid Material ◽  
Growth Cycle ◽  
Carbon And Nitrogen ◽  
Nitrogen Concentrations

A fungus, Aspergillus fumigatus Fres., which used bark as its sole carbon and energy source, was isolated. Difficulties arose in measuring fungal growth, since the hyphae and the bark could not be separated. Measurement of the weight loss of the solid material did not quantitatively estimate fungal growth. Therefore, two methods were developed to estimate fungal mass when the carbon and energy source is particulate and contributes to the parameter used as a measure of growth. They were based on determination of nitrogen either in the solid material or in the medium. The nitrogen concentration in A. fumigatus was found to be nearly constant throughout the growth cycle and to be independent of the carbon and nitrogen concentrations in the medium but to vary with the carbon source used.Aspergillus fumigatus was grown at 37C as a submerged culture in salts medium with finely ground bark from Picea abies as sole carbon and energy source. The bark medium was heat-sterilized before inoculation with spores. The fungus utilized cellulose and hemicellulose but not lignin. Substances solubilized from the bark contributed to the growth. The yield was the same on unextracted as on water-extracted bark, although growth was delayed on the former. Growth was rapid and comparable to growth on other polymeric polysaccharides, i.e. starch. Aspergillus fumigatus degraded 32–40% of the polymeric part of the bark within 4 days and with an economic coefficient of about 50%.

Factors affecting the urinary excretion of urea nitrogen in cattle. III.* High plasma urea nitrogen concentrations

1972 ◽  
Vol 23 (4) ◽  
pp. 727 ◽  
Author(s):  
RF Thornton ◽  
BW Wilson
Keyword(s):  
Nitrogen Concentration ◽  
High Plasma ◽  
Nitrogen Excretion ◽  
Urea Nitrogen ◽  
Plasma Urea ◽  
Factors Affecting ◽  
Linear Relationships ◽  
Nitrogen Concentrations ◽  
51Cr Edta ◽  
Filtered Load

The relationship between urinary urea nitrogen excretion, the filtered load of urea nitrogen, and the plasma urea nitrogen concentration were studied in cows intravenously infused with 0.5–10 g urea nitrogen per hour. These levels of infusion resulted in ranges of plasma urea nitrogen concentration of 12–63 mg/100 ml, of filtered urea nitrogen of 3–19 g/hr, and of urinary urea nitrogen excretion of 2.5–12.7 g/hr. There were linear relationships between the filtered load of urea nitrogen and the plasma urea nitrogen concentration (r = 0.91), between urinary urea nitrogen excretion and the filtered load of urea nitrogen (r = 0.92), and between urinary urea nitrogen excretion and the plasma urea nitrogen concentration (r = 0.97). It was concluded that the amount of urea excreted in the urine was regulated more by the plasma urea concentration than by renal processes such as glomerular filtration rate (GFR), the concentrating ability of the kidneys, or urine flow rate. The capacity of the bovine kidneys to excrete urea and mechanisms involved in the renal excretion of urea are discussed. The successful use of 51Cr-EDTA for the estimation of GFR in cattle is reported in an appendix. __________________ *Part II, Aust. J. Agric. Res., 21: 145 (1970).

Effect of seed weight, and seed phosphorus and nitrogen concentrations on the early growth of wheat seedlings

1990 ◽  
Vol 30 (4) ◽  
pp. 545 ◽  
Author(s):  
DGDe Marco ◽  
Marco DG De
Keyword(s):  
Seed Weight ◽  
Nitrogen Concentration ◽  
Seedling Emergence ◽  
Dry Weight ◽  
Early Growth ◽  
Sand Culture ◽  
Wheat Seedlings ◽  
P Content ◽  
Nitrogen Concentrations ◽  
High Concentrations

Wheat seeds of uniform weight selected from 3 batches of seed with phosphorus (P) concentrations of 0.14, 0.17 and 0.19% were sown in soil with a range of applied P treatments. Seedling emergence was more rapid the higher the seed P. By 25 days after sowing all plants grown at the highest concentration of applied P were similar in size, but with lower applied P, plants from seed with higher P concentrations had an advantage. A range of weight classes was selected from 2 batches of seed raised at low or high concentrations of P. There were 11 groups ranging in mean seed weight from 28 to 58 mg and in seed P from 0.13 to 0.37%. Seedlings from the high P batch had larger first leaves, a higher dry weight, and longer roots than those from the low P batch when grown in a highly P-deficient sand culture system. Within each batch, heavier seeds produced larger leaves, heavier plants and longer roots. The yield differences between batches were largely accounted for if the seed P content (�g P/seed) was considered rather than either percentage P or seed weight alone. Differences in seed nitrogen concentration or content did not consistently account for differences in seedling growth.

Effects of reproduction and artificial herbivory on vegetative growth and resource levels in deciduous and evergreen dwarf shrubs

1997 ◽  
Vol 75 (4) ◽  
pp. 656-666 ◽  
Author(s):  
Anne Tolvanen ◽  
Kari Laine
Keyword(s):  
Vegetative Growth ◽  
Nitrogen Concentration ◽  
Phenological Stages ◽  
Carbon And Nitrogen ◽  
Nitrogen Levels ◽  
Key Words Carbohydrate ◽  
Allocation Patterns ◽  
Carbohydrate Levels ◽  
Resource Levels ◽  
Vaccinium Myrtillus L

Effects of reproduction (production of flowers and berries) and artificial herbivory of different phenological stages on deciduous bilberry (Vaccinium myrtillus L.) and evergreen lingonberry (Vaccinium vitis-idaea L.) were investigated to determine differences in allocation patterns and recovery ability between these species. Vegetative growth and concentration of sugars, starch, and nitrogen were measured in ramets at the end of the growing season. Statistical comparisons were made between the treatments and damage times, but not between the species, as these were manipulated at different sites within the same forest. Reproduction reduced the vegetative growth significantly in lingonberry, whereas the fertile bilberry ramets grew even more than the sterile ones. The bilberry produced new shoots as a response to simulated herbivory, which significantly decreased carbohydrate levels in tissues. The evergreen lingonberry produced less shoots, and carbohydrate levels increased significantly. Nitrogen concentration either remained unchanged or increased in both species. For both species, time of damage affected the carbon and nitrogen levels as well as the ability to regrow. The species have developed different strategies to overcome the costs of sexual reproduction and herbivory damage, apparently as a result of the different resource allocation patterns and different architectural constraints for shoot development. The evergreen lingonberry grows slowly and conserves resources, whereas the deciduous bilberry allocates resources to increase the photosynthesizing biomass, which increases the potential of future survival, growth, and reproduction. Key words: carbohydrate, clipping, deblossoming, defoliation, nitrogen, Vaccinium.

scholarly journals Cultivation of Pleurotus sajor-caju on banana stalk and Bahia grass based substrates

2011 ◽  
Vol 29 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Félix G de Siqueira ◽  
Emerson T Martos ◽  
Romildo da Silva ◽  
Eustáquio S Dias
Keyword(s):  
Nitrogen Concentration ◽  
Biological Efficiency ◽  
Textile Mill ◽  
Physicochemical Factors ◽  
Nitrogen Concentrations ◽  
Pleurotus Sajor Caju ◽  
Bahia Grass ◽  
High Concentrations ◽  
Cultivation Substrate ◽  
Protein Rich

Banana stalks and Bahia grass were utilized as basic starting materials for the production of the mushroom Pleurotus sajor-caju. Banana stalks were combined with other waste or supplement products (wheat bran, coast-cross hay, bean straw and cotton textile mill) to obtain different nitrogen concentrations. Since Bahia grass is relatively rich in protein, it was combined with other substrates (banana stalk, coast-cross hay and bean straw) to maintain a substrate nitrogen concentration of about 1.5%. Banana stalks and Bahia grass were both more efficient in the production of the mushroom P. sajor-caju when utilized without the addition of other substrates, with biological efficiencies of 74.4% and 74.12%, respectively. When combined with other substrates or grasses, there was a drop in biological efficiency, independent of the concentration of nitrogen. Furthermore, the addition of protein-rich waste to banana stalks resulted in a decrease or absence of fructification, which indicates that high concentrations of nitrogen in the cultivation substrate may hinder the cultivation of this mushroom. On the other hand, results reveal that the ideal concentration of nitrogen may depend on other physicochemical factors and these factors may determine the success in cultivating P. sajor-caju. Therefore, we conclude that P. sajor-caju may be cultivated on banana stalk and Bahia grass as pure substrates, not being necessary their supplementation or combine them with another substrates.

Altitudinal variation in size and composition of eggs in the land snail Arianta arbustorum

1998 ◽  
Vol 76 (11) ◽  
pp. 2067-2074 ◽  
Author(s):  
A Baur ◽  
B Baur
Keyword(s):  
Clutch Size ◽  
Carbon Concentration ◽  
Egg Size ◽  
Nitrogen Concentration ◽  
Reproductive Investment ◽  
Land Snail ◽  
Dry Mass ◽  
Altitudinal Variation ◽  
Arianta Arbustorum ◽  
Carbon Concentrations

Egg size and egg provisioning are crucial for the survival of offspring in invertebrates without postlaying egg care. The effects of elevation and size of the mother on egg size and on nitrogen and carbon concentrations in eggs were examined in eight populations of the land snail Arianta arbustorum over an altitudinal gradient from 370 to 2340 m in Switzerland. The dry mass of single eggs ranged from 1.48 to 2.79 mg and decreased with increasing altitude. Adult snail size (shell volume) also decreased with increasing elevation as did clutch size and reproductive investment (clutch size x egg dry mass) of mothers. Hatching success of eggs varied among populations, but showed no altitudinal pattern. When differences in parental shell size were taken into account, correlations between altitude and egg size, clutch size, and reproductive investment disappeared. Thus, the altitudinal decreases in egg size, clutch size, and reproductive investment were mainly due to smaller snail sizes at higher elevations. The nitrogen concentration in eggs ranged from 3.4 to 4.5% and decreased with increasing elevation. In contrast to egg and clutch size, the altitudinal variation in nitrogen concentration of eggs cannot be explained by differences in snail size. The carbon concentration in eggs ranged from 31.1 to 33.1%, but showed no altitudinal variation. Nitrogen and carbon concentrations in the eggshell and egg fluid in eggs from four populations were examined separately. The nitrogen concentration in the eggshell ranged from 0.7 to 1.8% and was lower than that of the egg fluid (5.1-5.8%). Similarly, the carbon concentration in the eggshell was lower (20.2-22.8%) than that in the egg fluid (35.8-0.2%). Interpopulation differences in egg composition may affect growth, size, and survival of hatchlings and the extent of egg cannibalism in A. arbustorum.

scholarly journals Traffic Density-Related Black Carbon Distribution: Impact of Wind in a Basin Town

2021 ◽  
Vol 18 (12) ◽  
pp. 6490
Author(s):  
Borut Jereb ◽  
Brigita Gajšek ◽  
Gregor Šipek ◽  
Špela Kovše ◽  
Matevz Obrecht
Keyword(s):  
Black Carbon ◽  
Carbon Concentration ◽  
Ground Level ◽  
Traffic Density ◽  
Carbon Distribution ◽  
Wind Speeds ◽  
Black Carbon Concentration ◽  
Low Wind Speeds ◽  
Temperature Inversions ◽  
Carbon Concentrations

Black carbon is one of the riskiest particle matter pollutants that is harmful to human health. Although it has been increasingly investigated, factors that depend on black carbon distribution and concentration are still insufficiently researched. Variables, such as traffic density, wind speeds, and ground levels can lead to substantial variations of black carbon concentrations and potential exposure, which is even riskier for people living in less-airy sites. Therefore, this paper “fills the gaps” by studying black carbon distribution variations, concentrations, and oscillations, with special emphasis on traffic density and road segments, at multiple locations, in a small city located in a basin, with frequent temperature inversions and infrequent low wind speeds. As wind speed has a significant impact on black carbon concentration trends, it is critical to present how low wind speeds influence black carbon dispersion in a basin city, and how black carbon is dependent on traffic density. Our results revealed that when the wind reached speeds of 1 ms−1, black carbon concentrations actually increased. In lengthy wind periods, when wind speeds reached 2 or 3 ms−1, black carbon concentrations decreased during rush hour and in the time of severe winter biomass burning. By observing the results, it could be concluded that black carbon persists longer in higher altitudes than near ground level. Black carbon concentration oscillations were also seen as more pronounced on main roads with higher traffic density. The more the traffic decreases and becomes steady, the more black carbon concentrations oscillate.

Screening and Characterization of a Bacterium Capable of Simultaneous Heterotrophic Nitrification and Aerobic Denitrification at High Concentrations of Ammonia-Nitrogen

2014 ◽  
Vol 665 ◽  
pp. 487-490
Author(s):  
Te Wang ◽  
Zhao Xia Liu ◽  
Mei Juan Wu ◽  
Fu Hui Kang ◽  
Qing Chen ◽  
...  
Keyword(s):  
16S Rdna ◽  
Ammonia Nitrogen ◽  
Heterotrophic Nitrification ◽  
Denitrification Rate ◽  
Aerobic Denitrification ◽  
Optimal Conditions ◽  
Carbon And Nitrogen ◽  
Mass Ratios ◽  
High Concentrations ◽  
Denitrification Process

A bacterium capable of simultaneous heterotrophic nitrification and aerobic denitrification at high concentrations of ammonia-nitrogen was screened and identified and the denitrification property was investigated in this paper. The strain was isolated from aeration tank of wastewater disposed by activated sludge and analyzed and identified by 16S rDNA. The effects of different carbon sources and carbon and nitrogen mass ratios on denitrification rate were studied. The changes of various forms of ammonia-nitrogens during the simultaneous heterotrophic nitrification and aerobic denitrification process were characterized. A strain capable of simultaneous heterotrophic nitrification and aerobic denitrification at 600 mg/L nitrogen concentration has been isolated and screened. Comparison of its 16S rDNA sequence showed 100% similarity to Bacillus licheniformis strain Lr124/6. The strain was named as Bacillus sp. A22. The optimal conditions for degradation of ammonia-nitrogen by Bacillus sp. A22 were trisodium citrate as carbon source and carbon and nitrogen mass ratios of 10. The denitrification rate was 98.2% after 96 h of culture under the optimal conditions and there was hardly any intermediates accumulation in the denitrification process. It has practical applications that the denitrification can be performed efficiently at high concentrations of ammonia-nitrogen by method of simultaneous heterotrophic nitrification and aerobic denitrification by Bacillus sp. A22 in nitrogen purification treatment of wastewater with high concentrations of ammonia-nitrogen.

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