Leaf Anatomy of Wollemi Pine (Wollemia nobilis, Araucariaceae)

1999 ◽  
Vol 47 (5) ◽  
pp. 795 ◽  
Author(s):  
Geoffrey E. Burrows ◽  
Suzanne Bullock
Keyword(s):  
Leaf Anatomy ◽  
Microscope Level ◽  
Vascular Bundles ◽  
Abaxial Surface ◽  
Spongy Mesophyll ◽  
Oblique Orientation ◽  
Adult Morphology ◽  
Leaf Surfaces ◽  
Subsidiary Cells ◽  
Resin Canals

Leaves of adult morphology from Wollemi pine(Wollemia nobilis W.G.Jones, K.D.Hill & J.M.Allen)possess a thick cuticle, sunken stomata, abundant hypodermal fibres, distinctpalisade and spongy mesophyll with most palisade development on the adaxialside, compartmented cells, resin canals, sclereids, and vascular bundles withtransfusion tissue and a fibre cap abaxial to the phloem. Stomata are presenton both leaf surfaces, although in greater density on the abaxial surface, andusually have an oblique orientation and four or five subsidiary cells. At thelight microscope level, Araucaria can be distinguishedfrom Agathis as it possesses unusual compartmented cellsin the mesophyll, while Agathis does not. In addition,most Agathis species are hypostomatic, while mostAraucaria species have stomata on both the abaxial andadaxial surfaces. Thus W. nobilis has a leaf anatomywhich has a greater similarity to Araucaria than toAgathis.

scholarly journals Butia capitata (Mart.) Becc. lamina anatomy as a tool for taxonomic distinction from B. odorata (Barb. Rodr.) Noblick comb. nov (Arecaceae)

2015 ◽  
Vol 87 (1) ◽  
pp. 71-81 ◽  
Author(s):  
BRUNO F. SANT'ANNA-SANTOS ◽  
WELLINGTON G.O. CARVALHO JÚNIOR ◽  
VANESSA B. AMARAL
Keyword(s):  
Morphological Characteristics ◽  
Guard Cells ◽  
Plant Anatomy ◽  
Vascular Bundles ◽  
Epicuticular Waxes ◽  
Fibrous Layer ◽  
Middle Portion ◽  
Abaxial Surface ◽  
Adaxial Surface ◽  
Subsidiary Cells

The distinction between Butia capitata and B. odorata is based only on a few morphological characteristics, therefore there is a need for additional studies for supporting the separation of the species. As lamina anatomy characteristics are relevant in circumscribing Arecaceae taxa, this work aimed to describe B. capitata lamina anatomy and compare it with B. odorata. Samples from the middle portion of the pinnae were collected and processed in accordance with standard plant anatomy techniques. The epidermis is uniseriate and composed of a thick cuticle and epicuticular waxes into like hooked filaments. The subsidiary cells that arch over the guard cells are located at the hypodermis. The mesophyll is isobilateral and compact. The vascular bundles are collateral with a sclerenchymatic sheath extension that reaches the hypodermis. The stegmata cells have spherical and druse-like silica bodies. The midrib faces the adaxial surface with a thick fibrous layer surrounding the vascular bundles adjacent to the chlorenchyma. The stratified expansion tissue is on the abaxial surface, within the boundary between the mesophyll and midrib. Raphides are only found in B. capitata. Small bundles of the midrib fully surround the fibrous cylinder only in B. odorata. These characteristics are diagnostic and useful for supporting the proposed separation.

scholarly journals A study of epidermal leaf anatomy of 18 Euphorbia taxa from Kerman Province, Iran

Biologija ◽  
2017 ◽  
Vol 63 (2) ◽  
Author(s):  
Seyed Mehdi Talebi ◽  
Mitra Noori ◽  
Habibeh Afzali Naniz
Keyword(s):  
Cell Wall ◽  
Epidermal Cell ◽  
Leaf Anatomy ◽  
Cell Shape ◽  
Leaf Epidermis ◽  
Abaxial Surface ◽  
Leaf Epidermal Cell ◽  
Leaf Surfaces ◽  
Cell Shapes ◽  
Different Parts

Euphorbia is the largest genus of Euphorbiaceae widely distributed all over the world. The genus members grow naturally in different parts of Iran and nearly 96 species of Euphorbia have been listed in the country. Investigations show that the traits of foliar epidermis have taxonomic values. That is why the features of epidermal leaf anatomy of 18 Euphorbia taxa were studied in the present study. Plant samples were collected from Kerman Province, Iran, and identified using available references. Semi-permanent slides were prepared of adaxial and abaxial leaf epidermis. Then the slides were studied using light microscopy and some epidermal leaf anatomy characteristics stomata types, trichomes, the shape and type of epidermal cell, and their walls were examined. Photomicrographs were taken from each sample. Results showed that stomata type were stable among the species. Not only leaf epidermal cell shapes differed between the taxa, but also in some species they varied between the abaxial and adaxial surfaces. These conditions hold true for cell wall patterns. Some of the studied taxa had simple and uniseriate trichomes on the epidermal surfaces, in most of them trichomes were present on both leaf surfaces, while in one species trichomes were seen on the abaxial surface. Our findings confirmed that some of the anatomical traits, such as the absence or presence of trichomes, epidermal cell shape, and anticlinal cell wall patterns had taxonomic value and are useful in the identification of taxa.

Comparative morphoanatomical studies of south eastern Nigerian representatives of Oldenlandia L. (Rubiaceae)

2021 ◽  
Vol 20 (2) ◽  
pp. 107-118
Author(s):  
Chimezie Ekeke ◽  
Chinedum Alozie Oagzie ◽  
Josephine Agogbua
Keyword(s):  
Anatomical Structure ◽  
Vascular Bundles ◽  
Diagnostic Features ◽  
Spongy Mesophyll ◽  
Leaf Vein ◽  
Leaf Surfaces ◽  
Edo State ◽  
Endodermal Cells ◽  
Rivers State ◽  
Agricultural Farm

Leaf and stem anatomical structure of the four Oldenlandia L. (O. affinis (Roem. & Schult.) DC., O. corymbosa L., O. herbacea (Linn.) Roxb., and O. lancifolia (Schumach) DC.) from some parts viz. Ogbokor (Edo State), Obiga-Asa (Abia State), IITA staion Onne (Rivers State), and Agricultural farm Uniport (Rivers State) Nigeria were examined by light microscopy. The epidermal cells are pentagonal to polygonal with straight, curved or wavy anticlinal walls, and paracytic stomata. All the species have dorsiventral leaf with the leaf vein vascular bundles embedded in the spongy mesophyll. The midribs vascular bundles form an arc enclosed by parenchymatous endodermal cells. O. herabcea is amphistomatic while the other species are hypostomatic. Raphide bundles were seen only in the lamina of O. corymbosa. Tuft hair is absent in O. herbacea but occurred on the adaxial leaf surfaces of O. affinis, O. corymbosa, and O. diffusa. The stem of O. diffusa is terete while other species have quadrangular stem. Papillae occurred on the adaxial epidermis of O. affinis and O. corymbosa. The stem pith thickness (PT)/cortical thickness (ET) ratio varied among the species. Notable diagnostic features in these species include the PT/ET ratio, layers of cortex in the stem, occurrence of tuft hairs on the leaf veins and surface, presence or absence of raphides and papillose, layer of abaxial and adaxial cortex in the midrib, and amphistomatatic or hypostomatic leaf.

scholarly journals Leaf Structure and Taxonomy of Petunia and Calibrachoa (Solanaceae)

2002 ◽  
Vol 45 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Claudia dos Reis ◽  
Maria das Graças Sajo ◽  
João Renato Stehmann
Keyword(s):  
Leaf Anatomy ◽  
Morphological Differentiation ◽  
Identification Key ◽  
Leaf Structure ◽  
Vascular Bundles ◽  
Leaf Surfaces

We studied the leaf anatomy of sixteen species of Calibrachoa and eight species of Petunia. In Calibrachoa leaves, the vascular bundles sheath (endodermis) was formed by parenchymatous developed cells, different from those of the mesophyll. In Petunia, this sheath did not show a marked morphological differentiation. The Calibrachoa leaves could be separated according to the type of leaf margins, the distribution of the stomata on leaf surfaces, the organization of the mesophyll and the morphology of the trichomes. Based on these results, an indented dichotomous identification key was elaborated for the species of the genus Calibrachoa.

Reassessing species boundaries in the Syagrus glaucescens complex (Arecaceae) using leaf anatomy

Botany ◽  
2021 ◽  
pp. 379-387
Author(s):  
D.H.T. Firmo ◽  
S.A. Santos ◽  
M.E.M.P. Perez ◽  
P. Soffiatti ◽  
B.F. Sant’Anna-Santos
Keyword(s):  
Species Identification ◽  
Leaf Anatomy ◽  
Vascular System ◽  
Identification Key ◽  
Species Boundaries ◽  
Vascular Bundles ◽  
Geographical Isolation ◽  
Species Occurrence ◽  
Species Diagnosis ◽  
High Degree

The Syagrus glaucescens complex comprises three species: Syagrus glaucescens Glaz. ex Becc., Syagrus duartei Glassman, and Syagrus evansiana Noblick. Recently, a new population of S. evansiana that possesses a high degree of endemism was reported in the Serra do Cabral mountain. Here we intend to study the leaf anatomy of the S. glaucescens complex and confirm whether this newly found population (from now on called Syagrus aff. evansiana) belongs to S. evansiana or not. Specimens were collected to investigate their leaf anatomy, which showed distinct differences between S. aff. evansiana and S. evansiana. The midrib anatomy revealed novelties for the S. glauscecens complex, proving useful for species diagnosis. Features such as accessory vascular bundles around the vascular system of the midrib and the number of collateral bundles are diagnostic for species identification. In addition, morphological and anatomical analyses indicated a correlation with the species occurrence. We found greater similarity between S. glaucescens and S. duartei, while S. evansiana and S. aff. evansiana are more alike. Here, we propose a new identification key based only on the leaf anatomy. Despite their morphological similarities, S. aff. evansiana and S. evansiana presented differences in leaf anatomy, which — when associated with their geographical isolation — suggests a fourth taxon in the complex.

scholarly journals An Anatomical Study of Scilla (Scilloideae) Section Chionodoxa and Scilla bifolia in Turkey1

2017 ◽  
Vol 35 (0) ◽  
Author(s):  
H. YILDIRIM ◽  
K. YETISEN ◽  
A. ÖZDEMIR ◽  
C. ÖZDEMIR
Keyword(s):  
Numerical Analysis ◽  
Numerical Methods ◽  
Analysis Of Variance ◽  
Leaf Anatomy ◽  
Pearson Correlation ◽  
Anatomical Study ◽  
Anatomical Variations ◽  
Vascular Bundles ◽  
Single Row ◽  
Scilla Bifolia

ABSTRACT In the present study Scilla luciliae, S. forbesii, S. sardensis, S. siehei, Scilla x allenii and S. bifolia are compared anatomically. Some differences have been found in root, scape, and leaf anatomy of the taxa, and commented. S. luciliae, S. forbesii, S. sardensis and S. siehei have a metaxylem at the center of the root, others have 3-4 number metaxylem. Vascular bundles in two row in S. luciliae and S. forbesii in a single row in S. sardensis, Scilla x allenii and S. bifolia though in three rows in S. siehei. Aerenchyma tissue is present in mesophyll of five taxa leaf except S. sardensis. The anatomical variations in the taxa have been investigated by means of numerical methods (Analysis of variance and Pearson correlation). By the analysis of the investigated taxa from 12 anatomy related characters, it has been also found that the results from numerical analysis of anatomy characters can provide additional evidences, which correspond to the anatomy for the recognition of the taxa.

scholarly journals Leaf anatomy of a late Palaeozoic cycad

2017 ◽  
Vol 13 (11) ◽  
pp. 20170456 ◽  
Author(s):  
Zhuo Feng ◽  
Yong Lv ◽  
Yun Guo ◽  
Hai-Bo Wei ◽  
Hans Kerp
Keyword(s):  
Leaf Anatomy ◽  
Leaf Morphology ◽  
Yunnan Province ◽  
Southwest China ◽  
Upper Permian ◽  
Fossil Leaves ◽  
Leaf Surfaces ◽  
The Family ◽  
Clear Differentiation ◽  
Anatomical Evidence

Today, cycads are a small group of gymnospermous plants with a limited distribution in the (sub)tropics, but they were major constituents of Mesozoic floras. Fossil leaves sporadically found in latest Carboniferous and Permian floras have putatively been ascribed to cycads. However, their true affinity remains unclear due to the lack of anatomical evidence. Virtually all modern cycads have pinnate leaves, but this type of leaf morphology is by no means unique for cycads. Pinnate leaves of Plagiozamites oblongifolius Halle 1927 with well-preserved cuticles showing the epidermal anatomy are here described from the upper Permian Xuanwei Formation of Yunnan Province, Southwest China. The cuticles show a clear differentiation into costal and intercostal zones; stomata are confined to the intercostal zones on both the upper and lower leaf surfaces. The external morphology and the epidermal anatomy of these fossil leaves are closely comparable with those of extant cycads, particularly members of the family Zamiaceae.

Anatomy of Johnsongrass

Weed Science ◽  
1971 ◽  
Vol 19 (4) ◽  
pp. 385-393 ◽  
Author(s):  
C. G. McWhorter
Keyword(s):  
Sorghum Halepense ◽  
Vascular Bundles ◽  
Leaf Surfaces

The number of vascular bundles in the rhizomes of different ecotypes of johnsongrass (Sorghum halepense(L.) Pers.) varied from 71 to 154. The size of vascular bundles varied from 60 to 150 μ in an ecotype from California to 100 to 230 μ in an ecotype from Georgia. The size of xylem cells in these vascular bundles also varied widely, but the size of phloem cells was more constant. The number of vascular bundles in culms ranged from 43 to 123. The average number of vascular bundles in individual leaves varied from 46 to 158 for different ecotypes. The number of stomata on leaf surfaces deviated from 63 to 148/sq mm for 10 different ecotypes. An average of 95 stomata/sq mm occurred on the upper surface of johnsongrass leaves while an average of 113 stomata occurred on lower surfaces. The arrangement and size of stomata varied in different johnsongrass ecotypes.

Leaf Ultrastructure and δ13C Values of Three Seagrasses from the Great Barrier Reef

1976 ◽  
Vol 3 (1) ◽  
pp. 9 ◽  
Author(s):  
ME Doohan ◽  
EH Newcomb
Keyword(s):  
Great Barrier Reef ◽  
Leaf Anatomy ◽  
Co2 Assimilation ◽  
Land Plants ◽  
Vascular Bundles ◽  
Barrier Reef ◽  
Thalassia Hemprichii ◽  
Mesophyll Cells ◽  
Δ13c Values ◽  
Abundant Cytoplasm

Leaf anatomy, ultrastructure and 13C/12C ratios were studied in three species of seagrasses collected on the Great Barrier Reef: Cymodocea rotundata Ehrenb. & Hempr., C. serrulata (R. Br.) Aschers. & Magnus, and Thalassia hemprichii (Ehrenb.) Aschers. Although they belong to two different mono- cotyledonous families, the three species are quite similar in the characteristics studied. Cells of the epidermal layer of the leaves are extremely thick-walled and have abundant cytoplasm with large chloroplasts and numerous mitochondria. The chloroplast-microbody profile ratio is c. 4-5 : 1 and the mitochondrion-microbody ratio 10-15 : 1. The epidermal cells resemble transfer cells in having a pronounced development of ingrowths on the radial walls. The mesophyll cells have thin walls, a large central vacuole and a thin layer of cytoplasm with relatively few organelles. There is no specialization of mesophyll cells around the vascular bundles. The δ13C values for the three sea- grasses range from -6.90, to - 12.40, and thus are characteristic of C4 land plants, although the seagrasses do not conform to the C4 syndrome in leaf anatomy or ultrastructure. It is not possible to place the seagrasses in either the C3, C4 or crassulacean acid metabolism category of land plants, but whether they constitute yet a fourth group with respect to characteristics related to CO2 assimilation is not clear.

scholarly journals Taxonomic significance of some leaf anatomical features of the species of Annona L. (annonaceae) from Nigeria

2020 ◽  
Vol 22 (1) ◽  
pp. 101-112
Author(s):  
S.A. Adeniran ◽  
A.B. Kadiri ◽  
J.D. Olowokudejo
Keyword(s):  
Vascular Bundles ◽  
Taxonomic Significance ◽  
Anticlinal Wall ◽  
Abaxial Surface ◽  
Anatomical Features ◽  
Qualitative And Quantitative ◽  
Dichotomous Key ◽  
The Mean ◽  
Generic Constant ◽  
Existing Data

A comparative study of the some leaf anatomical features of four species of Annona occurring in Nigerian was undertaken with the aid of light microscope. The four foliar structures (epidermis, petiole, midrib and lamina architecture) studied revealed useful characters which support recognition of the species. A combination of these features has been used to prepare an artificial indented dichotomous key for identifying the  species. The generic constant features encountered included hypostomata, paracytic stomatal type, linear nerves endings, uneven midrib outline, and centrally located vascular bundles in the petiole and midrib. However, the most reliable distinguishing characters found across the species included presence of brachyparacytic stomata in A. reticulata, presence of trichomes on the midrib in A. senegalensis, absence of druses on the abaxial surface in A. muricata and A. squamosa, a thick pitted anticlinal walls on the surfaces of A. muricata and consistent polygonal areola shape in A. squamosa. The overlapping characters which also justify the closeness of the species and their grouping in a genus were recorded in both the qualitative and quantitative features. Prominent among them are the mean stomatal width which is about 1.0 μm in all species, nerve endings within the areole which varies between 1-2, U- or V-shaped midrib on the adaxial surface and straight to curved anticlinal wall pattern. The significance of these observations is discussed in updating the existing data in the genus. Keywords: Epidermis, Microscopy, Midrib, Petiole,   Systematic

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