Early Growth of Santalum album in Relation to Shade

1994 ◽  
Vol 42 (1) ◽  
pp. 83 ◽  
Author(s):  
DR Barrett ◽  
JED Fox

Morphological features of the hemi-parasite Santalum album Linn. (Indian sandalwood) were examined on tree seedlings raised under different shade treatments. Treatment levels were full sun and 32, 50, 70 and 80% shade. The level of shade significantly affected many morphological characteristics. Leaf area was least in full sun and greater under all shade levels. Leaves were thicker, shorter and narrower in full sun than in 80% shade. Leaf length/width ratio was greater when shade exceeded 50%. Petioles were shorter in 50% and more shade. Leaves in 80% shade had more chlorophyll and were of greater weight. Stomatal numbers were higher and internodes longer in 80% shade than in full sun. None of the following showed significant differences: plant heights, leaf numbers, crown widths and stem diameters. Chlorophyll concentrations are within the range of those possessed by non-parasitic land plants. On balance 80% shade plants had adaptive attributes more favourable to growth than plants from less shade. Survival was least in full sun.

2017 ◽  
Vol 7 (4) ◽  
pp. 432-439 ◽  
Author(s):  
Seyed Mehdi Talebi ◽  
Reza Rezakhanlou ◽  
A V. Matsyura

<p><em>Salvia multicaulis</em> is a widespread species of Lamiaceae family in Iran. There are many discussions about its infraspecific variations. Although some varieties were definite for this species in various parts of the world, no infraspecific taxon was reported in Iran and all samples of this species were named as S. multicaulis. In this study, morphological characteristics of S. multicaulis populations, naturally growing in Iran, was examined. Twenty-two traits were examined in 94 individuals of this species to<br />identify their phenotypic difference. Most of the investigated features were showing a high degree of variability, but it was highly pronounced for some characteristics such as basal leaf shape, basal leaf width, basal leaf length/ width ratio and basal petiole length. Significant positive/negative correlations were observed between some morphological variables. Furthermore, significant negative correlations were found between the eastern distribution of populations with basal leaf petiole length and basal leaf length/ width ratio. Based on the UPGMA cluster analysis, populations were divided into two main branches. The first branch contained four populations, while the second branch was bigger and clustered in two sub-branches. In one of them,<br />three populations and in another one the rest populations arranged in two groups. CA joined plot confirmed that each of studied populations or group of populations had distinct morphological trait(s), which were useful in identification of them. Our findings supported population no. 13 had unique morphological traits such as the largest bracts and basal leaf petiole, highest flower number of each inflorescence cycle, widest and largest calyx. The conservation of the highly diverse populations of<br />Iranian S. multicaulis is recommended.</p>


2001 ◽  
Vol 79 (10) ◽  
pp. 1248-1255 ◽  
Author(s):  
Loren Walker ◽  
Tara Sirvent ◽  
Donna Gibson ◽  
Nan Vance

Geographic differences among Hypericum perforatum L. plants in concentration of two hypericins and five morphological characteristics were analyzed in plants collected from four sites each in northern California and western Montana and two sites in Oregon. Differences among regional collections of H. perforatum were assessed based on analysis of hypericin and pseudohypericin concentration in floral, leaf, and stem tissue; light and dark leaf gland density; leaf area; leaf length/width ratio; and stem height. Significant differences in morphological and biochemical traits were detected primarily between samples collected from California and Montana. California samples had higher concentrations of hypericins, greater leaf gland density, larger leaves, and taller stems than those from Montana. Overall, Oregon samples did not consistently differentiate from those of Montana and California. Seasonal differences in hypericins were analyzed in Oregon plants only. Mean floral concentration of pseudohypericin (0.29%) and hypericin (0.06%) were highest during anthesis coinciding with July and August sampling dates, whereas mean leaf concentrations (0.19 and 0.04%, respectively) were highest in August.Key words: Hypericum perforatum, hypericin, St. John's wort, leaf glands, noxious weed, medicinal plant.


Author(s):  
Erwin Prastowo ◽  
Rina Arimarsetiowati

The coffee growth is considered to depend, partly, on the environmental condition at which they develop to accomplish both vegetative and generative stages. An exploratory survey in Lampung aimed at investigating the morphological characteristics of 13 Robusta coffee accessions growing on different altitudes.Local superior coffee clones were selected from eight farms, and subjected to identifications. Three key issues were concerned for both quantitatively, i.e. plant components of leaves, branches, and fruits, and qualitatively such as leaves and fruit characteristics, i.e. colour and shape. The analysis shows a similarity of about 60% of total qualitative variable identified among different accessions. The remaining characters are suggested to be more varying such as flush colour, leaf surface, ripe fruit colour, and stipule shape. Furthermore, a quantitative analysis showed a higher level of similarity for fruit characters, i.e. fruit length, -width, and -thickness, and to a slightly lower level for leaf characteristics, i.e. leaf length and -width, and number of productive branches. It was revealed that some variables, i.e. fruit weight; leaf and seed; and canopy characteristics, explaining the morphological variation of coffee throughout the accessions. Furthermore, cluster analysis may indicated a possible similarity of coffee morphologies either from area with different or the same environmental conditions. A high heterogeneity related to environmental conditions, genotypic variations, plant nutritional status, and agronomic practices, which unable to confirm in the present study, may limit the specific conclusions.


Plant Disease ◽  
2020 ◽  
Author(s):  
Mo Zhu ◽  
Jie Ji ◽  
Wenqi Shi ◽  
YongFang Li

Poa pratensis, known as bluegrass, is a perennial grass and one of the best varieties with highly valued pasture and turf grass uses. It is widely grown on golf courses and used for lawns in squares and parks (Luo et al. 2020). During April and May 2020, powdery mildew-like signs and symptoms were observed on leaves of P. pratensis in Muye Park, Xinxiang city (35.3°N; 113.9°E), Henan Province, China. White or grayish powdery masses in spots- or coalesced lesions were abundant on the adaxial surfaces of leaves and covered up to 90 % of the leaf area. Some of the mildew-infested leaves appeared chlorotic or began senescence. Mildew-infested leaves were collected to microscopically observe the morphological characteristics of this pathogen. Conidiophores were composed of foot cells, followed by one or two cells, and conidia. The ellipsoid- shaped conidia (n = 50) were 25 - 36 × 10 - 15 μm (length × width), on average 30 × 13 μm, with a length/width ratio of 2.3. Foot-cells (n = 15) were 30 - 44 μm long and 7 - 15 μm wide. On leaf surfaces, germinated conidia produced a short primary germ tube and then a long secondary germ tube that finally differentiated into a hooked appressorium. Chasmothecia were not found. Based on these morphological characteristics, the pathogen was initially identified as B. graminis f. sp. poae, the known forma specialis (f. sp.) of B. graminis on P. pratensis (Braun and Cook 2012; Troch et al. 2014). Mycelia of the pathogen were scraped from infected leaves and total genomic DNA was isolated using the method described previously (Zhu et al. 2019). The rDNA internal transcribed spacer (ITS) region was amplified applying primer pairs ITS1/ITS4 (White et al. 1990). The amplicon was cloned and sequenced by Invitrogen (Shanghai, China). The obtained sequence for the pathogen was deposited into GenBank under Accession No. MT892956 and was 100 % identical (549/549 bp) to B. graminis on P. pratensis (AB273530) (Inuma et al. 2007). In addition, the phylogenetic analysis clearly showed that the identified fungus and B. graminis f. sp. poae were clustered in the same branch. To perform pathogenicity analysis, leaf surfaces of eight healthy plants were inoculated by dusting fungal conidia from diseased leaves. Eight non-inoculated plants served as a control. The non-inoculated and inoculated plants were separately maintained in two growth chambers (humidity, 60 %; light/dark, 16 h/8 h; temperature, 18 ℃). Twelve to fourteen days after inoculation, B. graminis signs were visible on inoculated leaves, while control plants remained healthy. The pathogenicity assays were repeated twice and showed same results. Therefore, based on the morphological characteristics and molecular analysis, the pathogen was identified and confirmed as B. graminis f. sp. poae. This pathogen has been reported on P. pratensis in Switzerland and Japan (Inuma et al. 2007). This is, to our best knowledge, the first disease note reporting B. graminis on P. pratensis in China. Because the hybridization of B. graminis formae speciales (ff. spp.). allow the pathogens to adapt to new hosts, P. pratensis may serve as a primary inoculum reservoir of B. graminis to threaten other species, including cereal crops (Klingeman et al. 2018; Menardo et al. 2016). In addition, powdery mildew may negatively affect the yield and quality of grasses. Our report expands the knowledge of B. graminis f. sp. poae and provides the fundamental information for future powdery mildew control.


2020 ◽  
Vol 18 (2) ◽  
pp. e1003
Author(s):  
Eleonora Rodríguez-Polanco ◽  
Juan G. Morales ◽  
Melissa Muñoz-Agudelo ◽  
José D. Segura ◽  
Martha L. Carrero

Aim of study: To characterize isolates of Phytophthora sp. causing black pod rot (BPR) of cacao (Theobroma cacao L.).Area of study: Eight cocoa-growing regions in Colombia.Material and methods: Sixty isolates of Phytophthora sp. were obtained from tissues of cacao pods showing symptoms of BPR. Isolates were characterized using the morphology of sporangia and chlamydospores, molecular sequencing of regions of nuclear DNA (rDNA-ITS) and mitochondrial (COX) and virulence in different genotypes of cocoa pods.Main results: A high phenotypic variability between the isolates was determined, being the pedicel length and the length/width ratio (L/W) the most stable characters for species identification. Short pedicels with an average of 3.13 μm ± 0.28 and a length/width ratio of sporangia (L/W) with an average of 1.55 μm ± 0.11 were established as the most consistent morphological characteristics within palmivora species.Research highlights: Phytophthora pamivora was the only species associated to BPR, identified using morphology together with sequence analyses.


HortScience ◽  
2002 ◽  
Vol 37 (3) ◽  
pp. 585-592 ◽  
Author(s):  
Tae-Ho Han ◽  
Herman J. van Eck ◽  
Marjo J. De Jeu ◽  
Evert Jacobsen

An F1 population, derived from an intraspecific cross between two Alstroemeria aurea accessions, was used to map quantitative trait loci (QTL) involved in ornamental and morphological characteristics. One QTL for leaf length was mapped on linkage group three of both parents near marker E+ACCT/M+CGCA-I165 explaining 20% and 14.8% phenotypic variation. Two putative QTL were detected on leaf width on A002-3 and A002-6. One QTL and three putative QTL, involved in the leaf length/width ratio were identified accounting for 46.7% of the phenotypic variance in total. Significant interaction was observed between two QTL, S+AC/M+ACT-I162 and S+AC/M+AGA-I465 in a two-way analysis of variance (ANOVA). For the main color of the flower one QTL and putative QTL accounted for up to 60% of phenotypic variance suggesting simple genetic control of flower color. A two-way ANOVA of these QTL suggested an epistatic interaction. A QTL was detected for color of the inner side of outer lateral tepal with 26.5% of the phenotypic variance explained. This QTL was also associated with main color of the flower just below the 95% threshold value. Two QTL were detected with the Kruskal-Wallis test for the tip color of inner lateral tepal near QTL for other flower color traits. Consequently flower color traits were significantly correlated. A QTL and a putative QTL for the flower size was mapped near marker E+ACCG/M+CGCT-I193 and E+ACCG/M+CGCG-197, respectively. One putative QTL was detected for the stripe width of the inner lateral tepal.


2020 ◽  
pp. 1-9
Author(s):  
Rouzbeh Zangoueinejad ◽  
Mohammad Taghi Alebrahim ◽  
Te-Ming Tseng

Herbicide tolerance is commonly associated with reduced absorption and translocation of the herbicide; we hypothesized that the mechanism of dicamba tolerance in wild tomato (Solanum lycopersicum L.) accessions is due to these characteristics. The absorption and translocation of dicamba were investigated at a drift rate of 2.8 g a.e. ha−1 in three predetermined dicamba-tolerant (DT) wild accessions (TOM199, TOM198, and TOM300) and compared with two dicamba-susceptible (DS) commercial tomato cultivars [Money Maker (MM) and Better Boy (BB)]. Dicamba was quantified in three different parts of the tomato plant: two upper leaves, two lower leaves, and the roots at 1, 3, and 7 d after treatment. Both MM and BB absorbed more dicamba then all the three DT accessions. The overall translocation pattern of dicamba was similar between DS cultivars and DT accessions, thus suggesting that tolerance to dicamba in wild accessions may not be associated with reduced translocation but instead with reduced uptake of the herbicide. Additionally, reduced dicamba absorption in DT accessions may be attributed to their leaf characteristics, such as the presence of narrower leaves (3.42 leaf length/width ratio) and higher trichome density (20 no. mm−2) in DT accessions, than compared with DS cultivars (1.92 leaf length/width ratio and 8 no. mm−2 trichome density).


Plant Disease ◽  
2014 ◽  
Vol 98 (6) ◽  
pp. 847-847
Author(s):  
S. E. Cho ◽  
M. J. Park ◽  
J. H. Park ◽  
J. Y. Kim ◽  
H. D. Shin

Parsley, Petroselinum crispum (Mill.) Nyman, is a minor but important leaf crop in Korea. In June 2010, parsley plants (cv. Paramount) showing typical symptoms of powdery mildew were found with approximately 90% incidence (percentage of plants showing symptoms) in polyethylene-film-covered greenhouses in an organic farm in Icheon County of Korea. Symptoms first appeared as thin white colonies, which subsequently showed abundant growth on the leaves with chlorosis and crinkling. Most diseased plantings were unmarketable and shriveled without being harvested. The damage due to powdery mildew infections on parsley has reappeared in Icheon County and Gangneung City with confirmation of the causal agent made again in 2011 and 2012. Voucher specimens were deposited in the Korea University Herbarium (KUS). Appressoria on the mycelium were multilobed or moderately lobed. Conidiophores were cylindrical, 75 to 125 × 8 to 10 μm, straight in foot-cells, and produced conidia singly, followed by 2 to 3 cells. Conidia were oblong-elliptical to oblong, 32 to 55 × 14 to 20 μm with a length/width ratio of 1.7 to 2.9, lacked fibrosin bodies, and produced germ tubes on the perihilar position, with angular/rectangular wrinkling of the outer walls. First-formed conidia were apically conical, basally subtruncate to rounded, and generally smaller than the secondary conidia. Chasmothecia were not found. These structures are typical of the powdery mildew Pseudoidium anamorph of the genus Erysiphe. The specific measurements and morphological characteristics were consistent with those of E. heraclei DC. (1). To confirm the identity of the causal fungus, the complete ITS region of rDNA from isolate KUS-F25037 was amplified with primers ITS5 and P3 (3) and sequenced directly. The resulting 606-bp sequence was deposited in GenBank (Accession No. KF680162). A GenBank BLAST search of this sequence revealed 100% identity with that of E. heraclei on Anethum graveolens from Korea (JN603995) and >99% similarity with those of E. heraclei on Daucus carota from Mexico (GU252368), Pimpinella affinis from Iran (AB104513), Anthriscus cerefolium from Korea (KF111807), and many other parsley family (Apiaceae) plants. Pathogenicity was verified through inoculation by gently pressing diseased leaves onto leaves of five healthy potted parsley plants. Five non-inoculated plants served as negative controls. Inoculated plants developed symptoms after 7 days, whereas the control plants remained symptomless. The fungus present on the inoculated plants was morphologically identical to that originally observed on diseased plants. Parsley powdery mildew caused by E. heraclei has been known in Europe, North America, Brazil, and Japan (2,4). To our knowledge, this is the first report of powdery mildew infections by E. heraclei on parsley in Korea. Since cultivation of parsley was only recently started on a commercial scale in Korea, powdery mildew infections pose a serious threat to safe production of this herb, especially those grown in organic farming where chemical options are limited. References: (1) U. Braun and R. T. A. Cook. Taxonomic Manual of the Erysiphales (Powdery Mildews), CBS Biodiversity Series No. 11. CBS, Utrecht, 2012. (2) D. F. Farr and A. Y. Rossman. Fungal Databases. Syst. Mycol. Microbiol. Lab., Online publication, ARS, USDA, retrieved September 17, 2013. (3) S. Takamatsu et al. Mycol. Res. 113:117, 2009. (4) Y. Tsuzaki and K. Sogou. Proc. Assoc. Plant Prot. Shikoku 24:47, 1989.


Phytotaxa ◽  
2021 ◽  
Vol 482 (3) ◽  
pp. 279-290
Author(s):  
Dae-Hyun Kang ◽  
Homervergel G. Ong ◽  
Jung-Hoon Lee ◽  
Eui-Kwon Jung ◽  
Naing-Oo Kyaw ◽  
...  

Eriobotrya shanense, a new species from the karst region of Shan State, eastern Myanmar, is described and illustrated. This evergreen tree is similar to the autumn and winter-flowering, large-leaved species E. malipoensis and E. japonica by having tomentose hairs on the abaxial leaf surface, but can be distinguished by its obovate, widely obelliptic or oval blade shape, leaf length-width ratio of 2:1 (vs. 3:1), and rounded or obtuse apex (vs. acute). The species also resembles less-known, undercollected Myanmar broad-leaved species E. wardii and E. platyphylla in leaf shape and length-width ratio, but can be easily differentiated by the presence of tomentose hairs on the leaves, and inflorescence. Phylogenetic analysis based on nrDNA ITS region supported its close affinity with E. malipoensis and E. japonica. Molecular data also generally grouped the 17 congeneric taxa accessions in congruence to their leaf morphology, with the entire Eriobotrya clade strongly supported to be monophyletic and separate from Rhaphiolepis.


Plant Disease ◽  
2014 ◽  
Vol 98 (9) ◽  
pp. 1272-1272 ◽  
Author(s):  
C. A. D. Bragança ◽  
A. F. Nogueira Junior ◽  
F. Rogério ◽  
N. S. Massola

Barbados cherry, also called acerola, is a fruit originated from tropical America that is well-known for its high content of vitamin C and nutritional value. Anthracnose is one of the most common diseases on Barbados cherry. In Brazil, this disease is associated with Colletotrichum gloeosporioides sensu lato (2). In 2012, necrotic and sunken spots were observed on Barbados cherry fruit (cv. Rubra) in Sao Paulo State, Brazil, from which a Colletotrichum species was isolated on potato dextrose agar (PDA). The isolate was grown on PDA at 25°C and 12-h photoperiod under fluorescent light. The colony was gray on the upper surface and the reverse part was dark gray. Conidia (n = 50) were cylindrical to subcylindrical, hyaline, and 12 to 15 (avg. 12.7) × 3.8 to 5.9 (avg. 4.3) μm. Conidia length/width ratio was 2 to 3.6. Pathogenicity was confirmed on Barbados cherry fruit. Inoculation was carried out by depositing 40-μl droplets of a conidial suspension (1 × 105 conidia ml−1) on fruit wounded with a sterilized needle and on non-wounded fruit. Fruit were incubated in a moist chamber at 25°C. First symptoms appeared 3 and 5 days after inoculation on wounded and non-wounded fruit, respectively. No symptoms were observed on control fruit inoculated with water. Six isolates recovered from inoculated fruit showed the same morphological characteristics of the previous isolate. The DNA of the fungus was extracted by a CTAB protocol (1) and the sequences of ITS, GAPDH, ACT, CHS-1, TUB, and CAL genes (4) were generated. Sequences were used in BLAST searches in GenBank and were 100% similar to C. theobromicola, except for GAPDH. The ITS (KC566724) and CAL (KC566437) sequences matched strain ICMP 17099 (JX010285 and JX009588, respectively) with 100% identity. The BTUB (KC566148), GAPDH (KC566578), ACT (KC566870), and CHS-1(KC566292) sequences matched with the strains ICMP 18649 (JX010447, 100% identity), ICMP 17099 (JX009957, 99% identity, 1 pb), ICMP 18567 (JX009457, 100% identity), and ICMP 18613 (JX009771, 100% identity), respectively. The sequences were also compared with authentic culture of C. gloeosporioides (IMI 356878) and the identities were: ITS 99% (JX010148), CAL 91% (JX009729), BTUB 90% (JX010445), GAPDH 83% (GU174561), ACT 93% (JX009494), and CHS-1 98% (JX009747). Based on the multi-gene sequencing, the isolate was identified as C. theobromicola. C. theobromicola was described in 2010 (3) and it is considered as a widely distributed species occurring on different hosts in tropical and subtropical regions (4). This report shows the necessity of the identification of Colletotrichum species from tropical fruits to elucidate the etiology of anthracnose diseases of which C. gloeosporioides sensu lato is considered to be the causal agent. To our knowledge, this is the first report of C. theobromicola on Barbados cherry. References: (1) M. G. Murray and W. F. Thompson. Nucleic Acids Res. 8:4321, 1980. (2) R. Ritzinger et al. Acerola em Foco 13:1, 2007. (3) E. I. Rojas et al. Mycologia 102:1318, 2010. (4) B. S. Weir et al. Stud. Mycol. 73:115, 2012.


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