The interrelation of age, permeability and viability of subterranean clover seeds

The proportions of impermeable seeds, and the viability of permeable and impermeable seeds were measured in samples of Trifolium subterraneum seed that had been stored in a laboratory for up to 34 years. Viability of permeable seed decreased from 99 per cent after storage for 1 year to 8 per cent after 30 years. On the other hand, all impermeable seeds were viable for 1 to 5 years, and 83 per cent were viable after 30 years. The impermeable seed content of the older seed lots was generally higher than that of the younger ones. This difference was related to the high retention of impermeability of the seeds during storage, and to changes in the severity of threshing methods. The impermeable seed content was also correlated positively but poorly with the viability of both permeable and impermeable seeds, independent of the age of the seed. These associations are probably due to the common effect of the environment on all these variables during seed ripening. A small varietal component in each variable was detected. The implications of these data for the long-term storage of small samples of seed are discussed.

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Chlorogenic acid content in potato tubers with colored flesh as affected by a genotype, location and long-term storage

Plant Soil and Environment ◽

10.17221/195/2019-pse ◽

2019 ◽

Vol 65 (No. 7) ◽

pp. 355-360

Author(s):

Matyáš Orsák ◽

Karel Hamouz ◽

Jaromír Lachman ◽

Pavel Kasal

Keyword(s):

Chlorogenic Acid ◽

Acid Content ◽

Field Experiments ◽

The Other ◽

Potato Tubers ◽

Flesh Color ◽

Long Term Storage ◽

And Storage ◽

Term Storage

In three-year field experiments, the effect of genotype, flesh color, site conditions and storage on chlorogenic acid content (CAC) in tubers of potato cultivars with purple or red flesh was compared to yellow-fleshed cv. Agria. The results confirmed the significant effect of genotype on CAC. The highest CAC was characteristic on a three-year mean for the purple-fleshed cv. Vitelotte (769.5 mg/kg fresh weight (FW)), i.e. 1.19−2.6 times higher than in the other cultivars. In regard to the effect of flesh color, significantly higher mean CAC levels have been shown for the red-fleshed (2.8 times) and purple-fleshed (3.16 times) cultivars in comparison with cv. Agria (148 mg/kg FW). At the Uhříněves location with a warmer climate and frequent dry periods as compared to the second Valečov location, a higher CAC (1.18 times) was found. Cold storage (4°C, 6 months) resulted in a significant CAC increase varying from 33.2% in the Blaue St. Galler cultivar to 210.6% in the Vitelotte cultivar among all eight evaluated color-fleshed cultivars. On the other hand, the effect of storage on CAC was not evident in the yellow-fleshed Agria cultivar (inconclusive difference against CAC after harvest).

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Long term patterns of seed softening and seedling establishment from single seed crops of subterranean clover

Australian Journal of Experimental Agriculture ◽

10.1071/ea9840200 ◽

1984 ◽

Vol 24 (125) ◽

pp. 200 ◽

Cited By ~ 14

Author(s):

GB Taylor ◽

RC Rossiter ◽

MJ Palmer

Keyword(s):

Seedling Establishment ◽

Subterranean Clover ◽

Late Summer ◽

Trifolium Subterraneum ◽

The Other ◽

Hard Seed ◽

Single Seed ◽

One Year ◽

Seed Crops

Single seed crops of eight varieties of subterranean clover (Trifolium subterraneum) were grown in swards in four successive years in a medium rainfall environment at Bakers Hill, Western Australia. Measurements were made of seed production, the progress of seed softening within the first two summers, and residual hard seed and seedling numbers after the break of season for up to six successive regeneration years. Patterns of seed softening were also measured in a laboratory oven with a diurnal temperature fluctuation of 60/15�C. Approximately half the seeds of all varieties softened over the first summer and generally well over half the residual seeds softened during the second summer. Up to 18% of seeds of the hardest seeded variety, Northam, and 11% of Geraldton seeds persisted to the end of the third summer as hard seeds. Seed softening patterns showed a significant variety x regeneration year interaction due mainly to a slower rate of softening of Northam seeds relative to the other varieties after the first summer. In six of the varieties, seeds generally softened more slowly in the laboratory than in the field but in the case of the other two varieties this trend was reversed. Nevertheless, ranking of varieties for rate of seed softening was similar in field and laboratory. Seedling establishment ranged from 28 to 100% of soft seeds, depending on the incidence of late summer and autumn rains. It appeared that all varieties in the study would regenerate satisfactorily at densities of more than 15 plants/dm2 after at least one year in which little or no seed is set. Slower rates of softening would probably be desirable in drier regions or in rotation systems involving cropping.

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The Sowing Value of Tansy Phacelia (Phacelia Tanacetifolia Benth.) Seeds in the Long-Term Storage

Agricultural Engineering ◽

10.1515/agriceng-2016-0053 ◽

2016 ◽

Vol 20 (3) ◽

pp. 153-159 ◽

Cited By ~ 1

Author(s):

Piotr Ponichtera

Keyword(s):

Life Cycle ◽

Economic Life ◽

The Other ◽

Seed Germinability ◽

Long Term Storage ◽

Basic Parameters ◽

Economic Life Cycle ◽

Term Storage ◽

Phacelia Tanacetifolia

AbstractIn 2010 the experiment, which aim was to examine the sowing value of tansy phacelia seeds after eight and nine years of storage, was conducted in the laboratory of the Academy of Agrobusiness in Łomża. The experiment clearly demonstrates that the period of storage of tansy phacelia seeds has an essential impact on the changes of basic parameters of the sowing value. Vetrovska variety had the highest seed germinability among the other varieties of tansy phacelia which were stored for 8 years. At the same time this variety had the highest decrease in seed germinability after 9 years of storage. During the 9-year storage Natra variety showed the highest decrease in thousand seeds weight despite the highest value of this parameter among all the tested varieties immediately after yielding. The results; of the experiment allowed for the conclusion that the economic life: cycle of tansy phacelia seeds is 8 years.

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Evaluation of the Stability of Bacteriophages in Different Solutions Suitable for the Production of Magistral Preparations in Belgium

Viruses ◽

10.3390/v13050865 ◽

2021 ◽

Vol 13 (5) ◽

pp. 865

Author(s):

Hans Duyvejonck ◽

Maya Merabishvili ◽

Mario Vaneechoutte ◽

Steven de Soir ◽

Rosanna Wright ◽

...

Keyword(s):

Acinetobacter Baumannii ◽

Therapeutic Dose ◽

Bacterial Species ◽

The Other ◽

Manufacturing Processes ◽

High Importance ◽

Long Term Storage ◽

The Stability ◽

Term Storage

In Belgium, the incorporation of phages into magistral preparations for human application has been permitted since 2018. The stability of such preparations is of high importance to guarantee quality and efficacy throughout treatments. We evaluated the ability to preserve infectivity of four different phages active against three different bacterial species in five different buffer and infusion solutions commonly used in medicine and biotechnological manufacturing processes, at two different concentrations (9 and 7 log pfu/mL), stored at 4 °C. DPBS without Ca2+ and Mg2+ was found to be the best option, compared to the other solutions. Suspensions with phage concentrations of 7 log pfu/mL were unsuited as their activity dropped below the effective therapeutic dose (6–9 log pfu/mL), even after one week of storage at 4 °C. Strong variability between phages was observed, with Acinetobacter baumannii phage Acibel004 being stable in four out of five different solutions. We also studied the long term storage of lyophilized staphylococcal phage ISP, and found that the titer could be preserved during a period of almost 8 years when sucrose and trehalose were used as stabilizers. After rehydration of the lyophilized ISP phage in saline, the phage solutions remained stable at 4 °C during a period of 126 days.

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CDISC Standard-based Electronic Archiving of Clinical Trials

Methods of Information in Medicine ◽

10.3414/me9236 ◽

2009 ◽

Vol 48 (05) ◽

pp. 408-413 ◽

Cited By ~ 24

Author(s):

J. Aerts ◽

S. C. Semler ◽

C. Ohmann ◽

W. Kuchinke

Keyword(s):

Clinical Trial ◽

Clinical Trials ◽

Trial Data ◽

The Other ◽

Master File ◽

Long Term Storage ◽

Number Of Patients ◽

Term Storage ◽

Study Database

Summary Objectives: Our objectives were to develop, based on the analysis of archived clinical trial documents and data and on the requirements of GCP-compliant electronic archiving, a concept for legally secure and technically feasible archiving of the entire clinical trial, including the essential documents of the trial master file and the study database. Methods: Based on own experiences with CDISC, existing implementations and future developments, CDISC standards were evaluated concerning requirements for archiving clinical studies. Trial master files of a small, medium and large clinical study were analyzed to collect specifications for electronic archiving of records. Results: Two different ways of long-term storage exist for the clinical trial archive: document-oriented archival and data archiving of the study database. The trial master file has a highly complex structure; its different parts can vary greatly in size, depending of the working style of investigators, number of patients recruited, the number of adverse event reports and the number of queries. The CDISC standard ODM is especially suited for archiving clinical trials, because among other features it contains the entire clinical trial data and full audit trail information. On the other hand SDTM is a content standard suited for data warehouses. Two recent developments in CDISC will affect the archival of studies: the further development of ODM in the area of “eCRF submission” and the use of “Electronic Source Data”. Conclusions: The complexity and size of the trial master file requires new solutions. Though ODM provides effective means to archive the study database, it shows still deficiencies, especially for the joint archiving of data and the complex documentation of the trial master file. A concept was developed in which the ODM standard is part of an integrated archiving of the trial data and documents. ODM archiving of the study database enables long-term storage which is GCP-compliant. Archiving of documents of the trial master file in PDF/A, including links and electronic signatures, as well as the storage of selected study data in a data warehouse at the sponsor site in SDTM are the other components of the concept.

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An analysis of seed yield in some strains of subterranean clover (Trifolium subterraneum L.) when grown in binary mixtures

Australian Journal of Agricultural Research ◽

10.1071/ar9810445 ◽

1981 ◽

Vol 32 (3) ◽

pp. 445 ◽

Cited By ~ 2

Author(s):

RC Rossiter ◽

MJ Palmer

Keyword(s):

Competitive Advantage ◽

Seed Yield ◽

Subterranean Clover ◽

Trifolium Subterraneum ◽

The Other ◽

Pair Member ◽

Replacement Series ◽

Beginning Of Flowering ◽

Seed Yields

Two experiments using various strains of subterranean clover are described. The first experiment was conducted in the field with mixtures of Dwalganup/Northam A, DwalganupI/Daliak and Yarloop/Seaton Park, and the second in an open-sided glasshouse with Yarloop/Seaton Park. In the field experiment, the plots were grazed continuously from late July until early November when the swards were completely dry; in the glasshouse experiment, the swards were defoliated weekly until shortly after the beginning of flowering. Mixtures were arranged according to the de Wit replacement series. Seed yields for the Dwa1ganup/Northam A mixture showed the classical (de Wit) competition for the same resources, with Northam A the better competitor. On the other hand, for the Dwalganup/Daliak and Yarloop/Seaton Park mixtures there was no clear evidence of a competitive advantage of one strain over the other pair member, at least under our defoliated conditions. Whether or not this absence of 'competition' (as usually understood) is common in clover-strain mixtures is as yet unknown. We emphasize, however, that long-term success of clover strains in mixtures is not invariably associated with competitive advantage.

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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