scholarly journals Tissue Culture Propagation of Yam in Puerto Rico

1969 ◽  
Vol 67 (4) ◽  
pp. 419-428
Author(s):  
Amelia Cortés-Monllor ◽  
Lii-Jang Liu
Keyword(s):  
Tissue Culture ◽  
Puerto Rico ◽  
Indoleacetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Dioscorea Rotundata ◽  
Skoog Medium ◽  
Rooting Medium ◽  
Murashige And Skoog Medium ◽  
Tissue Culture Propagation

Rapid propagation of yam (Dioscorea rotundata cv. Habanero) was obtained from nodal segments cultured in modified Murashige and Skoog medium containing indoleacetic acid (IAA) and kinetin as establishment medium, and naphthaleneacetic acid (NAA) as rooting medium. The proliferation cycle, which takes approximately 2-3 months, increased four times the production of yam plantlets. These plantlets were successfully transferred to potting mixture and soil. This procedure is extremely useful for regenerating virusfree plantlets suitable for producing healthy tubers for planting.

Micropropagation of Scaevola —Australian native of ornamental horticulture

1998 ◽  
Vol 38 (4) ◽  
pp. 399 ◽  
Author(s):  
P. L. Bhalla ◽  
K. Sweeney
Keyword(s):  
Tissue Culture ◽  
Butyric Acid ◽  
High Survival ◽  
Root Initiation ◽  
Free Medium ◽  
Skoog Medium ◽  
Survival Percentage ◽  
Murashige And Skoog Medium ◽  
Ornamental Horticulture

Summary. A number of commercially available cultivars of Scaevola aemula, S. albida, S. phlebopetala, S. striata and material collected from the wild of S. glandulifera, S. hookeri and S. ramonissima were successfully propagated by tissue culture. Shoot segments 3–4 cm in length were multiplied in Murashige and Skoog medium without hormones. Addition of 25–150 µmol kinetin/L in the micropropagation medium of S. aemula and S. phlebopetala resulted in the formation of deformed shoots. Tissue cultured shoots rooted in hormone-free medium in 4–6 weeks. Indole-3-butyric acid (10–20 µmol/L) had an effect on rate of root initiation of S. phlebopetala but not on percentage of rooting. A high survival percentage (>95%) was obtained when plants were transferred to soil under glasshouse conditions indicating that micropropagation of Scaevola is feasible.

MICROPROPAGATION OF Helianthus maximiliani (Schrader) BY SHOOT APEX CULTURE / MICROPROPAGACION DE Helianthus maximiliani (Schrader) POR EL CULTIVO DE VAINAS DE LOS VASTAGOS / MICROPROPAGATION DE L’Helianthus maximiliani (Schrader) AU MOYEN DE LA CULTURE DE L’APEX DE LA POUSSE

Helia ◽  
2001 ◽  
Vol 24 (34) ◽  
pp. 63-68 ◽  
Author(s):  
Vasić Dragana ◽  
Škorić Dragan ◽  
Alibert Gilbert ◽  
Miklič Vladimir
Keyword(s):  
Silver Nitrate ◽  
Shoot Apex ◽  
Casein Hydrolysate ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Shoot Apices ◽  
Skoog Medium ◽  
Leaf Mesophyll ◽  
Murashige And Skoog Medium

SUMMARYH.maximiliani was micropropagated using culture of shoot apices on modified Murashige and Skoog medium (DV). Further propagation of in vitro grown plants was done by culture of their nodal segments and shoot tips on the same medium supplemented with phloridzin, silver nitrate and casein hydrolysate (DV'). Rooting was induced by dipping the explants into IBA solution prior culture. Viable protoplasts (90%) were isolated from leaf mesophyll. These protoplasts divided (18%) in culture in agarose droplets.

scholarly journals Optimation of Auxin and Cytokinin on Enhanced Quality and Weight of Coffea liberica Somatic Embryos

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Fitria Ardiyani ◽  
Edy Setiti Wida Utami ◽  
Hery Purnobasuki
Keyword(s):  
Somatic Embryo ◽  
Somatic Embryos ◽  
Dry Weight ◽  
Development Stage ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Coffea Liberica ◽  
Germination Stage

Coffea liberica is a variety of coffee that tolerant to marginal land, especially peatlands. One of propagation methods in C. liberica is somatic embryogenesis(SE) which producing large number of true-to-type plant seedlings in a short time. This research aimed at studying the effect of application of plant growthregulator (PGR) on quality and weight of somatic embryo of C. liberica. Somatic embryo in development stage was induced by Murashige and Skoog medium containing cytokinin as benzyl amino purin (BAP) and auxin as 2,4-dichlorophe-noxyacetic acid (2,4-D). While cotyledonary embryo in germination stage was induced by Murashige and Skoog medium containing cytokinin (BAP) and auxins as 2,4-D, indole acetic acid (IAA) and naphthaleneacetic acid (NAA). The resultsshowed that the application of auxins and cytokinins on development stage affected the formation of embryos, texture of calli, color of calli and embryos, and weight of somatic embryo. It also influenced the shoot and root formation, color and weight of geminating embryos of C. liberica at the germinating stage. During the development stage, addition of 1 mg/L BAP in the absence of 2,4-D in MS medium produced the highest quality of somatic embryo of C. liberica. This medium also produced heaviest somatic embryos but with lighter callus. While in germination stage, all medium treatments produced a typical germinating embryo. Coffea liberica germinating embryo growth optimally on MS medium containing 0.5 mg/L BAP as a single chemical or 0.5 mg/L BAP in combination with 0.5 mg/L IAA for shooting development. Whereas on rooting development, addition of 0.5 mg/L NAA on MS medium produced an optimal germinating embryo. Moreover, germination embryo of C. liberica recorded the highest in terms of dry weight on MS media with addition of 0.5 mg/L BAP. Application of appropriate concentration of auxin and cytokinin is needed to support the formation of somatic embryo and germinating embryo.

scholarly journals Establishment of Tissue Culture Regeneration System for Medicago ruthenica L. cv. ‘Zhilixing’

2021 ◽  
Author(s):  
Bo Xu ◽  
Rina Wu ◽  
Cuiping Gao ◽  
Fengling Shi
Keyword(s):  
Tissue Culture ◽  
Stress Resistance ◽  
Callus Induction ◽  
Combination Method ◽  
Induction Medium ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Medicago Ruthenica ◽  
Rooting Medium ◽  
Drought And Salt Tolerance

Background: Medicago ruthenica L. ‘Zhilixing’ is a new variety with superior forage and seed yield compared to the wild type. The cold, drought and salt tolerance of Zhlixing are better than those of alfalfa, suggesting that this variety can serve as a high-quality genetic resource for improving the stress resistance of alfalfa. However, because of the lack of tissue culture regeneration system, it is difficult to perform genetic transformation studies on stress resistance genes. This study aimed to establish an efficient tissue culture regeneration system for Zhilixing variety. Methods: Three types of explants were selected and tested on four types of basal media supplemented with different combinations of auxin and cytokinin for callus induction and differentiation, based on orthogonal tests to select the combinations of auxin and cytokinin suitable for callus induction and differentiation. Two-factor combination method was used to formulate a suitable rooting medium. Result: The hypocotyledonary axis was found to be an excellent explant for callus induction on MS medium. The optimum callus induction medium contained thidiazuron (TDZ, 0.5 mg/L), 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/L) and naphthaleneacetic acid (NAA, 0.5 mg/L) where the callus induction rate was 93.33%. The differentiation medium was supplemented with TDZ (0.75 mg/L), 2,4-D (0.25 mg/L) and 6-benzyladenine (6-BA, 1.5 mg/L) where the differentiation rate was 63.33%. Thidiazuron played the key role in both processes of callus induction and differentiation. Half-strength MS containing 0.1 mg/L of NAA was the most efficient rooting medium.

scholarly journals In Vitro Propagation of Pandoreas

HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 348-350 ◽  
Author(s):  
S. Latha Kancherla ◽  
Prem L. Bhalla
Keyword(s):  
Tissue Culture ◽  
Butyric Acid ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Shoot Induction ◽  
Indole Butyric Acid ◽  
Commercial Cultivars ◽  
Murashige And Skoog Medium

Pandoreas, Australian natives of horticultural significance, were successfully propagated using tissue culture. A protocol for rapid in vitro multiplication of commercial cultivars was developed using nodal segments cultured on Murashige and Skoog medium containing either BA or kinetin. Maximum shoot induction and number of shoots per explant for P. pandorana (Andrews) Steenis and P. jasminoides (Lindley) Schumann were on 8.8 μm BA and 4.6 μm kinetin. Higher levels of cytokinin in the medium inhibited shoot formation. Tissue-cultured shoots were rooted with IBA. This study demonstrates that Pandoreas can be successfully micropropagated. Chemical names used: 6-benzylaminopurine (BA); 3-indole butyric acid (IBA).

scholarly journals Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe

2001 ◽  
Vol 44 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Marcia O. Mello ◽  
Murilo Melo ◽  
Beatriz Appezzato-da-Glória
Keyword(s):  
Liquid Medium ◽  
Histological Analysis ◽  
Adventitious Shoots ◽  
Naphthaleneacetic Acid ◽  
Curcuma Zedoaria ◽  
Shoot Bud ◽  
Skoog Medium ◽  
Root Segments ◽  
Murashige And Skoog Medium

Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed on agar-solidified Murashige and Skoog medium without growth regulators at 25º C in the light. The establishment of these plantlets in soil was about 95%.

scholarly journals Micropropagation of Virginia Sweetspire (Itea virginica ‘Henry's Garnet’)

2003 ◽  
Vol 21 (4) ◽  
pp. 206-208
Author(s):  
Jon T. Lindstrom ◽  
Matthew C. Pelto
Keyword(s):  
Tissue Culture ◽  
Shoot Induction ◽  
Ex Vitro ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
One Year

Abstract The woody shrub, Itea virginica L., Virginia sweetspire, has recently increased in popularity due to its multiple seasons of interest in the landscape. In this study, we investigated micropropagation as a means to produce this plant. Combinations of BA (1, 4, and 10 μM) and NAA (0.01, 0.1, and 1.0 μM) were evaluated for in vitro shoot induction in Itea virginica L. ‘Henry's Garnet’ on a Murashige and Skoog medium. The best combination of BA and NAA (4 μM and 0.1 μM) yielded an average of 7.9 microshoots per explant for ‘Henry's Garnet’. When dipped in a common auxin-containing, commercial rooting formulation, microshoots rooted ex vitro within four weeks. Tissue-culture produced plantlets of I. virginica ‘Henry's Garnet’ flowered one year after removal from culture.

scholarly journals Germination and Regeneration of Plants from Old Cucumber Seed

HortScience ◽  
1991 ◽  
Vol 26 (7) ◽  
pp. 917-918 ◽  
Author(s):  
Nawab Ali ◽  
Robert Skirvin ◽  
Walter E. Splittstoesser ◽  
William L. George
Keyword(s):  
Relative Humidity ◽  
Cucumis Sativus ◽  
Naphthaleneacetic Acid ◽  
Skoog Medium ◽  
Cucumber Seed ◽  
Murashige And Skoog Medium

Cucumber (Cucumis sativus L.) seeds of `Marketer', `Marketmore', `Wisconsin SMR-18', `Tablegreen', `Spotfree', and `China' were stored at 3C and 38% relative humidity for up to 26 years. Seed older than 13 years did not germinate. Cultivars stored 10 years gave 80% germination, except Wisconsin SMR-18' (40%). Ten-year-old seeds were separated from their seedcoats, and cotyledons were excised into six segments. Explants were placed on Murashige and Skoog medium with all combinations of BAP (0, 1,2, and 3 mg·liter-1) and NAA (0, 0.1,0.2, and 0.3 mg·liter-1). Plants were obtained from culture for all cultivars grown on medium containing NAA and 1 mg BAP/liter. No plants were regenerated when BAP or NAA was lacking. Chemical names used: benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA).

scholarly journals 880 PB 270 In Vitro Flower and Plant Formation from Opuntia dillenii Haw. and Opuntia cochenillifera Mill.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560a-560
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Fresh Medium ◽  
Naphthaleneacetic Acid ◽  
Forage Crop ◽  
Skoog Medium ◽  
Number Of Species ◽  
Murashige And Skoog Medium ◽  
Opuntia Dillenii

The genus Opuntia includes a number of species which produce nutritious fruits and edible young joints, moreover, they are used as forage crop and for medicinal purposes. A procedure for flower and plant formation is described. Explants were excised from young flower receptacle and cultured on Murashige and Skoog medium (MS) supplemented with 0.9, 1.8, 4.5, or 9 uM thidiazuron and 0.5 uM naphthaleneacetic acid. Produced shoots were bisected longitudinally and cultured in a fresh medium from the same composition, other half shoots were subjected to a second cut by dividing transversely into distal and proximal explants before culture. About 80% explants of O. dellini produced flowers which produced shoots form the distal ales. only shoots were formed from O. cochenillifera explants. The distal explants produced more shoots than proximal explants. All formed shoots which attained 3 mm in length or longer were rooted directly in soil and all shoots formed roots and produced normal plants.

scholarly journals 878 PB 264 Micropropagation of pitaya (Hylocereus undatus Britt. et Rose)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 559f-559 ◽  
Author(s):  
Yasseen Mohamed-Yasseen
Keyword(s):  
Distal Part ◽  
Axillary Buds ◽  
Naphthaleneacetic Acid ◽  
Skoog Medium ◽  
Lateral Shoots ◽  
The Family ◽  
Murashige And Skoog Medium ◽  
Hylocereus Undatus ◽  
Number Of Shoots

Pltaya is a member of the family Caztacaae and the genus Hylocerus which has several species producing edible fruits. A procedure for micropropagation of pitaya using thidiazuron is described. Explants were excised from young joints of mature plants, and cultured on Murashige and Skoog medium (MS) containing 0.5 uM Thidiazuron (TDZ), and 3.5 uM naphthaleneacetic acid (NAA) Produced shoots were cut longitudinally into three explants or decapitated and cultured on MS supplemented with 0.01, 0.09, 0.5, or 0.9 uM TDZ with 0.5 uM NAA. Decapitated expiants produced shoots with higher frequency and number of shoots was higher than 1/3 explants. Shoots produced from decapitated explants were longer, thicker and vigorous, compared to shoots developed from 1/3 explants. Most shoots developed from, the distal part in both explants and produced several lateral shoots from axillary buds. Shoots were rooted in MS then transferred to soil and produced normal plants.

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