Determination of critical nitrogen concentrations of potato (Solanum tuberosum L. cv. Sebago) grown in sand culture

1992 ◽  
Vol 32 (6) ◽  
pp. 765 ◽  
Author(s):  
DO Huett ◽  
E White

A gamma x cubic response surface model was used to predict the dry matter yield of potato cv. Sebago over the 12-week growth period in sand culture with nitrogen (N) levels of 2, 7, 14, 29 and 43 mmol N/L. At each 2-week sampling period after emergence, dry matter yield relative to maximum was plotted against tissue N concentration to derive diagnostic petiole, petiole sap, leaf nitrate-N and leaf total N in youngest fully opened leaf (YFOL), youngest fully expanded leaf (YFEL) and oldest green leaf (OL) and for total N in bulked leaves. Critical concentrations corresponding to 90% maximum yield are presented. Tissue nitrate was much more responsive than leaf total N to applied N over the 2-14 mmol/L range where positive growth responses to N were recorded. Plants grown with 2 mmol N/L were severely N deficient and growth was depressed. Tissue nitrate concentrations in these plants from 4 weeks after emergence onwards were negligible, while leaf total N concentrations exceeded 2.36%. Salt toxicity occurred at 29 and 43 mmol NIL, and it sometimes reduced tissue N concentrations so that adequacy and toxicity concentrations overlapped. Critical tissue N concentrations declined over the growth period, the largest decline occurring for nitrate. Critical tissue N concentrations for YFEL, from 2 weeks after emergence to final harvest were: petiole sap nitrate-N, 1.2-0.2 g/L; petiole nitrate-N, 2.1-0.1%; leaf nitrate-N, 0.44-0.08%. Critical tissue nitrate concentrations clearly differentiated between inadequate and adequate N application levels. Critical leaf total N concentrations only differentiated between inadequate and marginal N application rates, except for OL when inadequate and marginally adequate (80-90% maximum yield) concentrations were not different (P>0.05). Nitrogen application level affected (P<0.05) leaf potassium, phosphorus, calcium (Ca), magnesium (Mg) and sulfur concentrations. The largest effects were recorded for Ca and Mg where increasing N application level reduced leaf nutrient concentration. Petiole sap nitrate concentrations can be used as a rapid field test for distinguishing between a deficient and an adequate N supply. Where concentrations exceed critical values, they can be interpreted as such because N fertiliser toxicity rarely occurs under field conditions.

1992 ◽  
Vol 32 (6) ◽  
pp. 759 ◽  
Author(s):  
DO Huett ◽  
E White

A gamma x cubic response surface model was used to predict the dry matter yield of lettuce cv. Montello over the 8-week growth period in sand culture with nitrogen (N) levels of 2, 5, 11, 18 and 36 mmol/L. At 1, 2, 3, 5, 7 and 8 weeks after transplanting, dry matter yield relative to maximum was plotted against tissue N concentration to derive diagnostic concentrations of petiole sap nitrate-N and leaf total N in youngest fully opened leaf (YFOL), youngest fully expanded leaf (YFEL) and oldest green leaf (OL), and total N in bulked leaf samples. Critical concentrations corresponding to 90% maximum yield are presented. Growth was consistently depressed at 2 mmol N/L due to N deficiency, and at 36 mmol N/L due to salt toxicity. Petiole sap nitrate concentrations were more responsive than leaf total N concentrations to N application levels. Leaf N concentrations at N application levels of 18 and 36 mmol/L were often similar. Critical leaf total N concentrations in YFOL and YFEL decreased from 2 weeks after transplanting to maturity, whereas the opposite trend occurred for petiole sap nitrate concentrations. Critical total N concentration ranges in YFEL were 0.30-0.95 g/L for petiole sap nitrate-N, and 4.00-5.30% for leaf total N concentration. Critical leaf total N and petiole sap nitrate concentrations clearly differentiated between inadequate and adequate N application rates. Critical values in most cases, differentiated toxic concentrations. Nitrogen application levels of 2 and 36 mmol N/L reduced (P<0.05) potassium, calcium and magnesium concentrations in all leaves. This confirms the importance of optimising N supply when determining critical levels of these nutrients for lettuce. Petiole sap nitrate-N concentrations, which can be determined rapidly in the field, can be used to distinguish between a deficient and an adequate N supply. The marked increase in critical concentration over the growth period requires consecutive determinations to verify the N status of lettuce.


1991 ◽  
Vol 31 (6) ◽  
pp. 835 ◽  
Author(s):  
DO Huett ◽  
E White

A gamma x quadratic response surface model was used to predict the growth rate over the 14-week growth period of zucchini squash (Cucurbita pepo L.) cv. Blackjack in sand culture with nitrogen (N) levels of 2, 7, 14, 29 and 43 mmol/L. Growth rate relative to maximum was plotted against tissue N concentration every 2 weeks, to derive diagnostic petiole sap; leaf nitrate-N and leaf total-N in youngest fully opened leaf, youngest fully expanded leaf and oldest green leaf; and total N in bulked leaf samples. Critical concentrations corresponding to 90% maximum growth rate for deficiency and toxicity are presented. Petiole sap and leaf nitrate-N were much more responsive than leaf total N concentrations over the 2-14 mmol N/L range where positive growth responses were recorded. At 2 mmol N/L, plants were severely N-deficient and growth rate was low (1.6 g/plant.week at fruit set). Tissue nitrate concentrations were negligible, while leaf total N concentrations exceeded 2.6%. Salt toxicity occurred at 29 and 43 mmol N/L, and at the highest N level, tissue N concentrations were sometimes reduced so that concentration ranges for adequacy and toxicity overlapped. Critical tissue N concentrations always exceeded (P<0.05) levels recorded in plants receiving a marginally deficient N level (7 mmol/L). Critical petiole sap and leaf nitrate-N concentrations were much more variable between sampling periods than leaf total N concentrations. Adequate concentration ranges (values between critical concentrations for deficiency and toxicity) were determined for the pre-fruit harvest (weeks 2-6) and fruit harvest (weeks 8-14) growth stages where values were common for consecutive weeks within each sampling period. It was only possible to determine adequate concentrations over the entire growth period for bulked leaf total N (4.30440% prefruit harvest and 4.15-4.45% fruit harvest). Concentrations of potassium (K), phosphorus and sulfur were affected (P<0.05) by N application level, with the largest effect being recorded for K. This confirms the importance of optimising N supply when determining critical levels of these nutrients for zucchini squash. Determination of petiole sap nitrate-N concentrations in the field can be used to distinguish between a deficient and an adequate N supply, but the large variation in values between sampling periods renders this technique less reliable than leaf total N. Tissue N concentrations which exceed critical deficient levels can be interpreted as such because they were recorded when growth was depressed at high N levels. This will rarely occur under field conditions.


1989 ◽  
Vol 29 (6) ◽  
pp. 883 ◽  
Author(s):  
DO Huett ◽  
G Rose

The cabbage cv. Rampo was grown in sand culture with 5 nitrogen (N) levels, between 2 and 43 mmol/L, applied as nitrate each day in a complete nutrient solution. The youngest fully opened leaf (YFOL), which became the wrapper leaf at heading, the youngest fully expanded leaf (YFEL) and the oldest green leaf (OL) were harvested at a minimum of 2-week intervals over a 12-week growth period. Standard laboratory leaf total N and nitrate-N determinations and rapid petiole sap nitrate-N determinations were conducted on YFOL, YFEL and OL. Total N was also determined in bulked leaves. The relationship between growth rate relative to the maximum at each sampling time and leaf N concentration was used to derive diagnostic petiole sap nitrate-N, leaf nitrate-N and total N in YFOL, YFEL and OL and bulked leaf total N concentrations. Critical concentration corresponded to 90% maximum growth rate and adequate concentration corresponded to 9 1-1 00% maximum growth rate. Petiole sap nitrate-N concentration, which can be measured rapidly in the field, and leaf nitrate-N concentration were very responsive to N application where positive growth responses were recorded. Critical N concentrations are presented for all leaves at most sampling times throughout the growth period. Critical total N concentrations in YFOL, YFEL and bulked leaves were higher during the pre-heading growth stage (weeks 2-6) than the post-heading growth stage (weeks 8-12). Critical N concentrations were inconsistent over the growth period and it was not possible to present single values to represent the full growth period, with 2 exceptions. A critical petiole sap nitrate-N concentration for OL of 3.0 g/L can be recommended for the full growth period because it represents a percentage of maximum growth rate range of 88-95%. Similarly, for YFEL, a critical total N concentration of 4.10% pre-heading (range 4.10-4.38%) represents a percentage maximum growth rate range of 62-90% and a post-heading critical total N concentration of 3.10% (range 3.10-3.50%) represents a percentage maximum growth rate range of 76-90%. The concentrations of potassium, phosphorus, calcium, magnesium and sulfur in YFOL, YFEL, OL and bulked leaf corresponding to N treatments producing maximum growth rates are also presented.


1988 ◽  
Vol 28 (3) ◽  
pp. 401 ◽  
Author(s):  
DO Huett ◽  
G Rose

The tomato cv. Flora-Dade was grown in sand culture with 4 nitrogen (N) levels of 1.07-32.14 mmol L-1 applied as nitrate each day in a complete nutrient solution. The youngest fully opened leaf (YFOL) and remaining (bulked) leaves were harvested at regular intervals over the 16-week growth period. Standard laboratory leaf total and nitrate N determinations were conducted in addition to rapid nitrate determinations on YFOL petiole sap. The relationships between plant growth and leaf N concentration, which were significantly affected by N application level, were used to derive diagnostic leaf N concentrations. Critical and adequate concentrations in petiole sap of nitrate-N, leaf nitrate-N and total N for the YFOL and bulked leaf N were determined from the relationship between growth rate relative to maximum at each sampling time and leaf N concentration. YFOL petiole sap nitrate-N concentration, which can be measured rapidly in the field by using commercial test strips, gave the most sensitive guide to plant N status. Critical values of 770-1 120 mg L-I were determined over the 10-week period after transplanting (first mature fruit). YFOL (leaf + petiole) total N concentration was the most consistent indicator of plant N status where critical values of4.45-4.90% were recorded over the 4- 12 week period after transplanting (early harvests at 12 weeks). This test was less sensitive but more precise than the petiole sap nitrate test. The concentrations of N, potassium, phosphorus, calcium and magnesium in YFOL and bulked leaf corresponding to the N treatments producing maximum growth rates are presented, because nutrient supply was close to optimum and the leaf nutrient concentrations can be considered as adequate levels.


1999 ◽  
Vol 133 (3) ◽  
pp. 263-274 ◽  
Author(s):  
J. VOS

In four field experiments, the effects of single nitrogen (N) applications at planting on yield and nitrogen uptake of potato (Solanum tuberosum L.) was compared with two or three split applications. The total amount of N applied was an experimental factor in three of the experiments. In two experiments, sequential observations were made during the growing season. Generally, splitting applications (up to 58 days after emergence) did not affect dry matter (DM) yield at maturity and tended to result in slightly lower DM concentration of tubers, whereas it slightly improved the utilization of nitrogen. Maximum haulm dry weight and N content were lower when less nitrogen was applied during the first 50 days after emergence (DAE). The crops absorbed little extra nitrogen after 60 DAE (except when three applications were given). Soil mineral N (0–60 cm) during the first month reflected the pattern of N application with values up to 27 g/m2 N. After 60 DAE, soil mineral N was always around 2–5 g/m2. The efficiency of N utilization, i.e. the ratio of the N content of the crop to total N available (initial soil mineral N+deposition+net mineralization) was 0·45 for unfertilized controls. The utilization of fertilizer N (i.e. the apparent N recovery) was generally somewhat improved by split applications, but declined with the total amount of N applied (range 0·48–0·72). N utilization and its complement, possible N loss, were similar for both experiments with sequential observations. Separate analysis of the movement of Br− indicated that some nitrate can be washed below 60 cm soil depth due to dispersion during rainfall. The current study showed that the time when N application can be adjusted to meet estimated requirements extends to (at least) 60 days after emergence. That period of time can be exploited to match the N application to the actual crop requirement as it changes during that period.


2007 ◽  
Vol 47 (8) ◽  
pp. 927 ◽  
Author(s):  
M. D. A. Bolland ◽  
I. F. Guthridge

For the first time, we quantified pasture dry matter (DM) responses to applied fertiliser nitrogen (N) for intensively grazed, rain-fed, dairy pastures on sandy soils common in the Mediterranean-type climate of south-western Australia. The pastures are composed of subterranean clover (Trifolium subterraneum L.) and annual and Italian ryegrass (Lolium rigidum Gaud. and L. multiflorum Lam.). Six rates of N, as urea (46% N), were applied to 15 m by 15 m plots four times during 2002 and after each of the first 5–7 grazings in 2003 and 2004, throughout the typical April–October growing season. Total rates of N applied in the first year of the experiments were 0, 60, 120, 160, 200 and 320 kg N/ha, which were adjusted in subsequent years as detailed in the ‘Materials and methods’ section of this paper. The pastures in the experiments were rotationally grazed, by starting grazing when ryegrass plants had 2–3 leaves per tiller. The amount of pasture DM on each plot was measured before and after each grazing and was then used to estimate the amount of pasture DM consumed by the cows at each grazing for different times during the growing season. Linear increases (responses) of pasture DM to applied N occurred throughout the whole growing season when a total of up to 320 kg N/ha was applied in each year. No maximum yield plateaus were defined. Across all three experiments and years, on average in each year, a total of ~5 t/ha consumed DM was produced when no N was applied and ~7.5 t/ha was produced when a total of 200 kg N/ha was applied, giving ~2.5 t/ha increase in DM consumed and an N response efficiency of ~12.5 kg DM N/kg applied. As more fertiliser N was applied, the proportion of ryegrass in the pasture consistently increased, whereas clover content decreased. Concentrations of nitrate-N in the DM consistently increased as more N was applied, whereas concentrations of total N, and, therefore, concentration of crude protein in the DM, either increased or were unaffected by applied N. Application of N had no effect on concentrations of other mineral elements in DM and on dry matter digestibility and metabolisable energy of the DM. The results were generally consistent with findings of previous pasture N studies for perennial and annual temperate and subtropical pastures. We have shown that when pasture use for milk production has been maximised in the region, it is profitable to apply fertiliser N to grow extra DM consumed by dairy cows; conversely, it is a waste of money to apply N to undergrazed pastures to produce more unused DM.


2020 ◽  
Author(s):  
Jiftah Ben-Asher

&lt;p&gt;The first Nc dilution curve was based on dry matter (DM) power function. This model is limited to&amp;#160; point of singularity near zero. Another disadvantage was that it required meaasurements of DM which is time and labor consuming. Alternatively we proposed a logistic model that starts at zero and on the abscissa assumed a linear relationship between days after emergence (DAE) and DM throughout the relevant stages of wheat growth cycle. &amp;#160;&lt;/p&gt;&lt;p&gt;The Objectives of this study were to: 1) To demonstrate the feasibility of digital camera to replace laboratory tests. 2) To Determine critical N (Nc) and Nitrogen nutrition Index(NNI) of spring wheat and 3) Use N% and dry matter yield in order to calculate N uptake by wheat. This last is expected to be a tool to calculate the required amount of nitrogen to obtain maximum yield.&lt;/p&gt;&lt;p&gt;Wheat experiments were conducted in greenhouse lysimeters. Varied rates of N fertilizer (equivalent to 0&amp;#8211;180 kg ha&lt;sup&gt;-1&lt;/sup&gt;) and several&amp;#160; cultivars varying from shortest to longest ripening growth period. Nc reduced gradually from about 6% to 2%&amp;#160; ( =60-20 gr/Kg) when DM increased with DAE&amp;#160; from 0 to 14,000 kg/ha during 80 growing days.&amp;#160; NNI was stable and clearly distinct between &amp;#160;&amp;#160;maximal index (1.0 &amp;#160;and minimal index (0.2) when (DAE) was about 60;&amp;#160;&amp;#160; Photographs succeeded to replicate laboratory measurements and obtained a linear regression curve with a unity&amp;#160; slop and r&lt;sup&gt;2&lt;/sup&gt;=0.93. Nitrogen.&amp;#160; use efficiency (NUE) ranged from 50 to 65 kg&amp;#160; DM/unit N and from 30 to 50 Kg grain /unit N&amp;#160;.&lt;/p&gt;


1977 ◽  
Vol 57 (3) ◽  
pp. 763-770
Author(s):  
H. T. KUNELIUS ◽  
MICHIO SUZUKI

Frode orchard grass (Dactylis glomerata L.) was fertilized with 99–495 kg N/ha/yr in three equal applications and harvested three or four times per season over a 3-yr period to determine the productivity, quality of forage and persistence of stands. The application of N resulted in significant (P =.001) linear and quadratic increases in dry matter (DM) yields. Higher DM yields were obtained with the 3-harvest system while the yield distribution within the season was more uniform for the 4-harvest system. Total N concentrations of orchard grass increased linearly with the N rates. Total N yields were dependent on the rates of applied N with the recovery of applied N ranging from 39 to 70% at 99–297 kg N/ha/yr, respectively. The in vitro disappearance of DM was slightly reduced by the high N rates in the 1st and 2nd harvests. The nitrate-N concentrations were highest in the early and late summer ranging from.11 to.29% at 297–495 kg N/ha/yr, respectively. The persistence of orchard grass was better under the 4- than the 3- harvest system.


2008 ◽  
Vol 59 (7) ◽  
pp. 624 ◽  
Author(s):  
J. L. Jacobs ◽  
G. N. Ward

The 2-pond system to treat and contain dairy effluent is commonplace on dry-land dairy farms in southern Australia. The first pond is a deep anaerobic treatment pond and the second a shallow aerobic pond where the liquid effluent is stored. This liquid effluent contains a range of nutrients that have the potential to influence forage dry matter (DM) yields, herbage nutritive characteristics, and mineral content of forages. The effect of applying second-pond dairy effluent to a summer turnip (Brassica rapa L.) crop over two consecutive summer periods was measured. In addition to the application of effluent, N fertiliser was also applied. Effluent was applied at three rates, 0, 30, and 60 mm ~7–8 weeks after turnips were sown each year, with fertiliser N applied at either 0, 25, 50, or 75 kg N/ha in combination with effluent rates immediately before effluent application. Turnips were assessed for DM accumulation, nutritive characteristics, and mineral and nitrate-N content. Effluent contained high concentrations of both potassium (K) (440–500 kg/ML) and sodium (Na) (637–766 kg/ML), with moderate levels of calcium (Ca) (177–180 kg/ML) and magnesium (158–213 kg/ML). Total N was higher in Year 2 (208 kg/ML) than in Year 1 (160 kg/ML), with the proportion of total N present as ammonia-N also higher in Year 2 (81%) than in Year 1 (57%). Dry matter yield responses for leaf and root were 20 and 11 kg DM/ha per effluent mm applied in Year 1 and 19 and 13 kg DM/ha.mm applied in Year 2, respectively. Total DM yield increases were 32 and 39 kg DM/ha.mm applied for Years 1 and 2, respectively. There was no effect of N application or interaction between effluent and N application in either year. For Year 1, nutritive characteristics were relatively unaffected by either effluent or N fertiliser application, while in Year 2, leaf crude protein content increased (P < 0.05) in a linear manner at 0.058% per mm effluent applied. The K and Na content of turnip leaves increased (P < 0.05) with effluent application in both Years 1 and 2, while the Ca decreased (P < 0.05) with effluent application in Year 2. Results from this study further emphasise the potential value of second-pond dairy effluent to increasing forage DM yield and improving the nutritive value of turnips. The data, however, question the value of using N fertiliser on its own or in combination with effluent to improve the same attributes. Dry matter yield responses to effluent were similar across both years despite contrasting climatic conditions, highlighting the ability of turnips to respond to limited moisture inputs.


1995 ◽  
Vol 43 (4) ◽  
pp. 419-433
Author(s):  
H. Biemond ◽  
J. Vos ◽  
P.C. Struik

Three greenhouse trials and one field trial were carried out on Brussels sprout cv. Icarus SG2004 in which the treatments consisted of different N amounts and application dates. DM and N accumulation in stems, apical buds and groups of leaf blades, petioles and sprouts were measured frequently throughout crop growth. Total amounts of accumulated DM and N were affected by amount of N applied and date of application, but the final harvest indexes for DM and N (0.10-0.35 and 0.20-0.55, respectively) were not significantly affected by treatments in most experiments. Nitrate N concentrations were only high (up to about 2%) shortly after planting. The total N concentration of leaf blades and petioles increased with increasing leaf number. This increase resulted from a decreasing N concentration during the leaf's life. The total N concentration in sprouts changed little with leaf number.


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