Assessment of the nitrogen and potassium status of irrigated Brussels sprouts (Brassica oleracea L. var. gemmifera) by plant analysis

1996 ◽  
Vol 36 (7) ◽  
pp. 887 ◽  
Author(s):  
CMJ Williams ◽  
NA Maier
Keyword(s):  
Total Yield ◽  
Leaf Age ◽  
Plant Analysis ◽  
Total N ◽  
Brussels Sprouts ◽  
Critical Concentrations ◽  
N Supply ◽  
Nitrate N ◽  
N Concentration ◽  
N Status

Four field experiments were carried out during 1992-93 (sites 1 and 2) and 1993-94 (sites 3 and 4) to assess the effects of nitrogen (N), at rates up to 600 kgha, and potassium (K), at rates up to 300 kgha, on total N, nitrate-N and K concentrations in petioles of the youngest fully expanded leaves (P-YFEL) of Brussels sprouts (Brassica oleracea var. gemmifera). The experiments were located in commercial plantings in the Mt Lofty Ranges, South Australia. Plant samples were collected at 2-4-week intervals from 4 to 28 weeks after the plants were transplanted. Temporal or seasonal variation, and the effects on concentrations of total N, nitrate-N and K of sampling leaves next in age (YFEL-1 to YFEL+2) to the index leaf, were also studied. Total N concentration in P-YFEL was more sensitive to variations in N supply than nitrate-N at all sites. Total N and nitrate-N concentrations in petioles also varied with the age of the leaf sampled. Total N concentrations in petioles of leaves sampled 4-16 weeks after transplanting decreased with increasing leaf age. In contrast, nitrate-N concentrations in petioles sampled 4-8 weeks after transplanting increased with leaf age. Potassium concentrations in petioles did not vary consistently between leaves of different age. From 4 to 6 weeks after transplanting, relationships between total N or nitrate-N concentrations in P-YFEL and relative total yield were not significant (P>0.05), therefore, critical concentrations could not be determined. Linear and quadratic models were used to study the relationships between total N and nitrate-N concentration in P-YFEL and relative total yield during 8-28 weeks after transplanting. Total N concentrations accounted for a greater amount of variation in relative total yield at 10, 12, 14, 16, 18, 20, 24 and 28 weeks after transplanting compared with nitrate-N. Coefficients of determination (r2) were in the range 0.52-0.93. Relationships between nitrate-N concentration in P-YFEL and relative total yield were only significant 8, 10, 14 and 16 weeks after transplanting and 9 values were in the range 0.49-0.82. Critical concentrations for total N decreased from 3.13-3.44% at 10 weeks to 1.22-1.38% at 28 weeks after transplanting. This decrease highlights the importance of carefully defining sampling time to ensure correct interpretation of plant test data. Potassium concentrations also decreased between 4 and 28 weeks after transplanting. Critical concentrations were not determined for K, because the crops at all sites did not respond significantly (P>0.05) to applied K. Based on sensitivity (as indicated by the range in tissue concentrations in response to variations in N supply) and on the correlations between total N and nitrate-N concentrations and relative total yield, we concluded that total N was better than nitrate-N as an indicator of plant N status and yield response of Brussels sprouts. We suggested that growers sample P-YFEL several times during the growing season, starting 10 weeks after transplanting. Plant analysis can be used to monitor N status and to detect N deficiencies which may arise during the growing season of Brussels sprouts which may be up to 9 months duration. Growers can adjust their fertiliser N program to ensure deficiencies are quickly corrected.

Diagnostic nitrogen concentrations for tomatoes grown in sand culture

1988 ◽  
Vol 28 (3) ◽  
pp. 401 ◽  
Author(s):  
DO Huett ◽  
G Rose
Keyword(s):  
Critical Values ◽  
Sand Culture ◽  
Nutrient Concentrations ◽  
Total N ◽  
Nitrate N ◽  
N Concentration ◽  
Leaf N Concentration ◽  
Petiole Sap ◽  
N Status ◽  
Leaf N

The tomato cv. Flora-Dade was grown in sand culture with 4 nitrogen (N) levels of 1.07-32.14 mmol L-1 applied as nitrate each day in a complete nutrient solution. The youngest fully opened leaf (YFOL) and remaining (bulked) leaves were harvested at regular intervals over the 16-week growth period. Standard laboratory leaf total and nitrate N determinations were conducted in addition to rapid nitrate determinations on YFOL petiole sap. The relationships between plant growth and leaf N concentration, which were significantly affected by N application level, were used to derive diagnostic leaf N concentrations. Critical and adequate concentrations in petiole sap of nitrate-N, leaf nitrate-N and total N for the YFOL and bulked leaf N were determined from the relationship between growth rate relative to maximum at each sampling time and leaf N concentration. YFOL petiole sap nitrate-N concentration, which can be measured rapidly in the field by using commercial test strips, gave the most sensitive guide to plant N status. Critical values of 770-1 120 mg L-I were determined over the 10-week period after transplanting (first mature fruit). YFOL (leaf + petiole) total N concentration was the most consistent indicator of plant N status where critical values of4.45-4.90% were recorded over the 4- 12 week period after transplanting (early harvests at 12 weeks). This test was less sensitive but more precise than the petiole sap nitrate test. The concentrations of N, potassium, phosphorus, calcium and magnesium in YFOL and bulked leaf corresponding to the N treatments producing maximum growth rates are presented, because nutrient supply was close to optimum and the leaf nutrient concentrations can be considered as adequate levels.

scholarly journals Effects of nitrogen accumulation and partitioning of dry matter and nitrogen of vegetables. 1. Brussels sprouts

1995 ◽  
Vol 43 (4) ◽  
pp. 419-433
Author(s):  
H. Biemond ◽  
J. Vos ◽  
P.C. Struik
Keyword(s):  
Dry Matter ◽  
Leaf Number ◽  
Nitrogen Accumulation ◽  
N Accumulation ◽  
Total N ◽  
Brussels Sprouts ◽  
Apical Buds ◽  
Nitrate N ◽  
N Concentration ◽  
Final Harvest

Three greenhouse trials and one field trial were carried out on Brussels sprout cv. Icarus SG2004 in which the treatments consisted of different N amounts and application dates. DM and N accumulation in stems, apical buds and groups of leaf blades, petioles and sprouts were measured frequently throughout crop growth. Total amounts of accumulated DM and N were affected by amount of N applied and date of application, but the final harvest indexes for DM and N (0.10-0.35 and 0.20-0.55, respectively) were not significantly affected by treatments in most experiments. Nitrate N concentrations were only high (up to about 2%) shortly after planting. The total N concentration of leaf blades and petioles increased with increasing leaf number. This increase resulted from a decreasing N concentration during the leaf's life. The total N concentration in sprouts changed little with leaf number.

Critical nitrate-nitrogen and total nitrogen concentrations for vegetative growth and seed yield of Linola (edible-oil linseed) as affected by plant age

1995 ◽  
Vol 35 (2) ◽  
pp. 239 ◽  
Author(s):  
PJ Hocking
Keyword(s):  
Seed Yield ◽  
Vegetative Growth ◽  
Edible Oil ◽  
Plant Age ◽  
Main Stem ◽  
Stem Tissue ◽  
Total N ◽  
N Supply ◽  
Nitrate N ◽  
N Status

Edible-oil linseed (Linola, CSIRO Australia) was grown in a sand culture experiment in a glasshouse to develop tissue tests for assessing the nitrogen (N) status of the crop. Seven rates of N, provided as nitrate, were used to obtain critical N concentrations. Plants were tissue-tested at 3 developmental stages: early tillering (TL), flower buds visible (BV), and the start of flowering (SF). Suitable tissues for tests based on nitrate-N were the upper half of the main stem and the whole main stem. Leaves were unsuitable as their nitrate-N concentration was unresponsive to N supply until well above the rate for maximum growth. For tests based on total N, suitable tissues were upper stem, upper leaves, total stem, total leaves, and whole shoot. Critical N supply rates for vegetative growth at TL, BV, and SF, respectively, were 85, 145, and 145 mg/L. The critical N supply rate for seed yield was 65 mg/L. Excessive N supplies (350, 700 mg N/L) reduced both seed oil percentage and seed yield. Critical nitrate-N concentrations in fresh, upper stem tissue for vegetative growth decreased from-0.26 to 0.16 mg/g fresh weight (FW) between stages TL and BV. A critical nitrate-N concentration for seed yield could only be obtained for fresh stem tissue at TL, and this value was 50% lower than that for vegetative growth. Critical nitrate-N concentrations [mg/g dry weight (DW)] in dried stem tissue for vegetative growth at TL, BV, and SF, respectively, were 2.3, 1.7, and 0.7 (upper stem); and 2.1, 1.1, and 0.6 (whole stem). Critical nitrate N values (mg/g DW) for seed yield at TL, BV, and SF were 1.1, 0.8, and 0.3 (upper stem); and 1.0,0.7, and 0.2 (whole stem). Critical total N concentrations (% DW) for vegetative growth at TL, BV, and SF, respectively, were 3.0, 2.3, and 2.2 (upper stem); 5.3, 5.8, and 4.5 (upper leaves); 2.2, 1.7, and 1.6 (whole stem); 5.5, 4.9, and 4.5 (total leaves); and 4.5, 3.1, and 2.7 (whole shoot). Corresponding total N values (% DW) for seed yield at TL, BV, and SF, respectively, were 2.9, 2.2, and 2.0 (upper stem); 5.2, 4.8, and 4.3 (upper leaves); 2.1, 1.4, and 1.4 (whole stem); 5.2, 4.4, and 4.2 (total leaves); and 4.3,2.8, and 2.6 (whole shoot). The upper stem is the preferred tissue when testing for nitrate-N, and the whole shoot is the most convenient tissue for total N. Tissue testing for N status of Linola needs to be matched closely to plant age or stage of development because of the decline in critical N concentrations between early tillering and flowering.

Determination of critical nitrogen concentrations of lettuce (Lactuca sativa L. cv. Montello) grown in sand culture

1992 ◽  
Vol 32 (6) ◽  
pp. 759 ◽  
Author(s):  
DO Huett ◽  
E White
Keyword(s):  
Dry Matter ◽  
Growth Period ◽  
Sand Culture ◽  
N Application ◽  
Total N ◽  
N Supply ◽  
Nitrate N ◽  
N Concentration ◽  
Nitrate Concentrations ◽  
Petiole Sap

A gamma x cubic response surface model was used to predict the dry matter yield of lettuce cv. Montello over the 8-week growth period in sand culture with nitrogen (N) levels of 2, 5, 11, 18 and 36 mmol/L. At 1, 2, 3, 5, 7 and 8 weeks after transplanting, dry matter yield relative to maximum was plotted against tissue N concentration to derive diagnostic concentrations of petiole sap nitrate-N and leaf total N in youngest fully opened leaf (YFOL), youngest fully expanded leaf (YFEL) and oldest green leaf (OL), and total N in bulked leaf samples. Critical concentrations corresponding to 90% maximum yield are presented. Growth was consistently depressed at 2 mmol N/L due to N deficiency, and at 36 mmol N/L due to salt toxicity. Petiole sap nitrate concentrations were more responsive than leaf total N concentrations to N application levels. Leaf N concentrations at N application levels of 18 and 36 mmol/L were often similar. Critical leaf total N concentrations in YFOL and YFEL decreased from 2 weeks after transplanting to maturity, whereas the opposite trend occurred for petiole sap nitrate concentrations. Critical total N concentration ranges in YFEL were 0.30-0.95 g/L for petiole sap nitrate-N, and 4.00-5.30% for leaf total N concentration. Critical leaf total N and petiole sap nitrate concentrations clearly differentiated between inadequate and adequate N application rates. Critical values in most cases, differentiated toxic concentrations. Nitrogen application levels of 2 and 36 mmol N/L reduced (P<0.05) potassium, calcium and magnesium concentrations in all leaves. This confirms the importance of optimising N supply when determining critical levels of these nutrients for lettuce. Petiole sap nitrate-N concentrations, which can be determined rapidly in the field, can be used to distinguish between a deficient and an adequate N supply. The marked increase in critical concentration over the growth period requires consecutive determinations to verify the N status of lettuce.

scholarly journals LEAF TOTAL NITROGEN CONCENTRATION AS AN INDICATOR OF NITROGEN STATUS FOR PLANTLETS AND YOUNG PLANTS OF EUCALYPTUS CLONES

2015 ◽  
Vol 39 (4) ◽  
pp. 1127-1140 ◽  
Author(s):  
Eric Victor de Oliveira Ferreira ◽  
Roberto Ferreira Novais ◽  
Bruna Maximiano Médice ◽  
Nairam Félix de Barros ◽  
Ivo Ribeiro Silva
Keyword(s):  
Total Nitrogen ◽  
Block Design ◽  
Nitrogen Concentration ◽  
Soil N ◽  
Total N ◽  
Chlorophyll Meter ◽  
N Concentration ◽  
Total Nitrogen Concentration ◽  
N Status ◽  
Leaf N

The use of leaf total nitrogen concentration as an indicator for nutritional diagnosis has some limitations. The objective of this study was to determine the reliability of total N concentration as an indicator of N status for eucalyptus clones, and to compare it with alternative indicators. A greenhouse experiment was carried out in a randomized complete block design in a 2 × 6 factorial arrangement with plantlets of two eucalyptus clones (140 days old) and six levels of N in the nutrient solution. In addition, a field experiment was carried out in a completely randomized design in a 2 × 2 × 2 × 3 factorial arrangement, consisting of two seasons, two regions, two young clones (approximately two years old), and three positions of crown leaf sampling. The field areas (regions) had contrasting soil physical and chemical properties, and their soil contents for total N, NH+4-N, and NO−3-N were determined in five soil layers, up to a depth of 1.0 m. We evaluated the following indicators of plant N status in roots and leaves: contents of total N, NH+4-N, NO−3-N, and chlorophyll; N/P ratio; and chlorophyll meter readings on the leaves. Ammonium (root) and NO−3-N (root and leaf) efficiently predicted N requirements for eucalyptus plantlets in the greenhouse. Similarly, leaf N/P, chlorophyll values, and chlorophyll meter readings provided good results in the greenhouse. However, leaf N/P did not reflect the soil N status, and the use of the chlorophyll meter could not be generalized for different genotypes. Leaf total N concentration is not an ideal indicator, but it and the chlorophyll levels best represent the soil N status for young eucalyptus clones under field conditions.

Dissolved carbon and nitrogen leaching following variable logging-debris retention and competing-vegetation control in Douglas-fir plantations of western Oregon and Washington

2009 ◽  
Vol 39 (8) ◽  
pp. 1484-1497 ◽  
Author(s):  
Robert A. Slesak ◽  
Stephen H. Schoenholtz ◽  
Timothy B. Harrington ◽  
Brian D. Strahm
Keyword(s):  
Soil Water ◽  
Dissolved Organic Nitrogen ◽  
Organic Nitrogen ◽  
Total N ◽  
Vegetation Control ◽  
Dissolved Carbon ◽  
Competing Vegetation ◽  
Nitrate N ◽  
N Concentration ◽  
C And N

We examined the effect of logging-debris retention and competing-vegetation control (CVC, initial or annual applications) on dissolved organic carbon (DOC), dissolved organic nitrogen, and nitrate-N leaching to determine the relative potential of these practices to contribute to soil C and N loss at two contrasting sites. Annual CVC resulted in higher soil water nitrate-N concentration and flux, with the magnitude and duration of the effect greatest at the high-N site. Most of the increase in nitrate-N at the low-N site occurred in treatments where logging debris was retained. Dissolved organic nitrogen increased at the high-N site in March of each year following annual CVC, but the contribution of this increase to total N concentration was small (2%–4% of total N flux). There was no effect of logging-debris retention or CVC treatment on soil water DOC concentrations, indicating that DOC inputs from logging debris and competing vegetation were either retained or consumed in the mineral soil. The estimated increase in leaching flux of dissolved C and N associated with the treatments was low relative to total soil pools, making it unlikely that loss of these elements via leaching will negatively affect future soil productivity at these sites.

scholarly journals Use of a Chlorophyll Meter for Nondestructive and Rapid Estimation of Leaf Tissue Nitrogen in Macadamia

2019 ◽  
Vol 29 (3) ◽  
pp. 308-313 ◽  
Author(s):  
Russell Galanti ◽  
Alyssa Cho ◽  
Amjad Ahmad ◽  
Javier Mollinedo
Keyword(s):  
Linear Regression ◽  
Leaf Tissue ◽  
Sampling Period ◽  
Concentration Data ◽  
Total N ◽  
Chlorophyll Meter ◽  
N Concentration ◽  
Important Concern ◽  
N Management ◽  
N Status

Nitrogen (N) management in macadamia (Macadamia integrifolia) orchards is an important concern for growers. Leaf tissue analysis is the accepted method for determining N status in macadamia; however, this process is expensive and time-consuming. The chlorophyll meter has been used in other crops to estimate N status in plants through estimation of the amount of chlorophyll in leaf tissue. The use of the chlorophyll meter in two macadamia cultivars (Kakea and Kau) at two locations in Hawai’i (Kapa’au and Pahala) and five time periods (12 Apr. 2017, 13 June 2017, 15 June 2017, 18 Dec. 2017, and 20 Feb. 2018) was assessed. Leaf samples were collected based on a tissue-sampling protocol, chlorophyll meter (SPAD) values were collected, and leaves were analyzed for total N concentration. Data were analyzed statistically using linear regression. Leaf tissue N concentration had a positive monotonic relationship to SPAD values for both macadamia cultivars, both locations, and all sampling periods. The sampling period of Apr. 2017 for ‘Kakea’ macadamia had the greatest R2 value for the linear regression at 0.85. The Feb. 2018 sampling period had an R2 value for the linear regression of 0.74. ‘Kau’ macadamia had the greatest R2 value for the linear regression of 0.24 in the Dec. 2017 sampling period. The slopes of the two macadamia cultivars for June 2017 were different from each other, suggesting that N recommendations need to be customized for specific macadamia cultivars if sampled in summer. The chlorophyll meter can be used for general estimation of tissue N in macadamia. Additional methods need to be considered and researched to refine procedures for direct estimation of total N concentration when using the chlorophyll meter.

Nitrate‐N and Total N Concentration Relationships in Several Plant Species 1

1976 ◽  
Vol 68 (4) ◽  
pp. 556-560 ◽  
Author(s):  
G. L. Terman ◽  
J. C. Noggle ◽  
C. M. Hunt
Keyword(s):  
Plant Species ◽  
Total N ◽  
Nitrate N ◽  
N Concentration

Determination of critical nitrogen concentrations of zucchini squash (Cucurbita pepo L.) cv. Blackjack grown in sand culture

1991 ◽  
Vol 31 (6) ◽  
pp. 835 ◽  
Author(s):  
DO Huett ◽  
E White
Keyword(s):  
Growth Rate ◽  
Growth Period ◽  
Sand Culture ◽  
Total N ◽  
N Supply ◽  
Nitrate N ◽  
Fruit Harvest ◽  
Petiole Sap ◽  
Leaf Nitrate

A gamma x quadratic response surface model was used to predict the growth rate over the 14-week growth period of zucchini squash (Cucurbita pepo L.) cv. Blackjack in sand culture with nitrogen (N) levels of 2, 7, 14, 29 and 43 mmol/L. Growth rate relative to maximum was plotted against tissue N concentration every 2 weeks, to derive diagnostic petiole sap; leaf nitrate-N and leaf total-N in youngest fully opened leaf, youngest fully expanded leaf and oldest green leaf; and total N in bulked leaf samples. Critical concentrations corresponding to 90% maximum growth rate for deficiency and toxicity are presented. Petiole sap and leaf nitrate-N were much more responsive than leaf total N concentrations over the 2-14 mmol N/L range where positive growth responses were recorded. At 2 mmol N/L, plants were severely N-deficient and growth rate was low (1.6 g/plant.week at fruit set). Tissue nitrate concentrations were negligible, while leaf total N concentrations exceeded 2.6%. Salt toxicity occurred at 29 and 43 mmol N/L, and at the highest N level, tissue N concentrations were sometimes reduced so that concentration ranges for adequacy and toxicity overlapped. Critical tissue N concentrations always exceeded (P<0.05) levels recorded in plants receiving a marginally deficient N level (7 mmol/L). Critical petiole sap and leaf nitrate-N concentrations were much more variable between sampling periods than leaf total N concentrations. Adequate concentration ranges (values between critical concentrations for deficiency and toxicity) were determined for the pre-fruit harvest (weeks 2-6) and fruit harvest (weeks 8-14) growth stages where values were common for consecutive weeks within each sampling period. It was only possible to determine adequate concentrations over the entire growth period for bulked leaf total N (4.30440% prefruit harvest and 4.15-4.45% fruit harvest). Concentrations of potassium (K), phosphorus and sulfur were affected (P<0.05) by N application level, with the largest effect being recorded for K. This confirms the importance of optimising N supply when determining critical levels of these nutrients for zucchini squash. Determination of petiole sap nitrate-N concentrations in the field can be used to distinguish between a deficient and an adequate N supply, but the large variation in values between sampling periods renders this technique less reliable than leaf total N. Tissue N concentrations which exceed critical deficient levels can be interpreted as such because they were recorded when growth was depressed at high N levels. This will rarely occur under field conditions.

The effect of interval between harvests and nitrogen application on the concentration of nitrate-nitrogen in the total herbage, green leaf and ‘stem’ of grasses

1986 ◽  
Vol 106 (3) ◽  
pp. 467-475 ◽  
Author(s):  
D. Wilman ◽  
P. T. Wright
Keyword(s):  
Field Experiment ◽  
Perennial Ryegrass ◽  
Nitrate Nitrogen ◽  
Italian Ryegrass ◽  
Green Leaf ◽  
N Application ◽  
Total N ◽  
Nitrate N ◽  
N Concentration ◽  
Time Of Year

SummaryThe effect of six intervals between harvests and three levels of N application on the concentration of nitrate-N and total N in total herbage, green leaf and ‘stem’ was studied in two varieties of perennial ryegrass during 30-week periods in each of the first two harvest years of a field experiment. The effect of two intervals between harvests on the concentration of nitrate-N in Italian ryegrass total herbage was studied in the same experiment. The effect of two intervals between harvests and three levels of N application on the concentrations of nitrate-N and total N in total herbage was studied in five grasses during a 32-week period in a second field experiment.Increasing the interval between harvests tended to increase the concentration of nitrate-N in herbage; however, this seemed due mainly to the average date of harvest being later in the year with the longer intervals. The concentration of nitrate-N in herbage increased from June to September. Italian and hybrid ryegrass and tall fescue were much higher than perennial ryegrass in nitrate-N concentration at the highest level of applied N (525 kgN/ha per year). Apart from the species and time of year effects, the nitrate-N concentration seemed to be determined mainly by the amount of N applied divided by the number of days between the date of application and the date of sampling. The ‘stem’ of perennial ryegrasa tended to be slightly higher in nitrate-N concentration than green leaf. The proportion of nitrate-N in total N was increased by increasing the interval between harvests and by applying N and was nearly twice as high in ‘stem’ as in green leaf. Both the nitrate-N and the total N concentration of herbage, particularly the latter, seemed to be inversely related to solar radiation receipt.

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