Integrated 1H NMR-based metabolomics analysis of earthworm responses to sub-lethal Pb exposure

2016 ◽  
Vol 13 (5) ◽  
pp. 792 ◽  
Author(s):  
Ting Chen ◽  
Yan Liu ◽  
Ming-Hui Li ◽  
Hua-Dong Xu ◽  
Ji-Yang Sheng ◽  
...  
Keyword(s):  
Heavy Metals ◽  
1H Nmr ◽  
Nitrate Solution ◽  
Metabolic Changes ◽  
Warning Signs ◽  
Toxic Effects ◽  
Great Promise ◽  
Orthogonal Signal Correction ◽  
Pb Exposure ◽  
Biochemical Assessment

Environmental contextHeavy metals are non-degradable and are therefore a severe and persistent environmental menace. The toxic effects of Pb were investigated using NMR to determine the metabolic changes in earthworms exposed to Pb. The approach using 1H NMR to analyse earthworm metabolomics demonstrated great potential as a reliable, rapid and convenient tool to assess the toxicity of heavy metals and could be used to identify warning signs of heavy metal contamination of soil. AbstractA 1H nuclear magnetic resonance (NMR)-based approach to metabolomics combined with atomic absorption spectroscopy, histopathological examination and biochemical assessment was used to determine the toxic effects of lead (Pb) on earthworms (Eisenia fetida). Earthworms were exposed to Pb in a lead nitrate solution at converted concentrations of 1.25, 5.0 and 20µgcm–2 in contact tests for 48h. Based on histopathological inspection, the epidermis, muscles, chloragogenous tissues and intestinal epithelium were severely impaired. Based on biochemical assessment, a disruption of the antioxidative system and neurotoxic effects in earthworms occurred following exposure to Pb. Orthogonal signal correction–partial least-squares-discriminant analysis of NMR profiles indicated that Pb exposure in earthworms caused widespread metabolic changes, which were associated with oxidative stress, neurotransmitter imbalance, disruption of osmotic equilibrium and interference in energy metabolism and nucleic acid metabolism. The integrated metabolomics approach provided new insights into Pb-induced toxicity in earthworms. Metabolomics is a powerful and highly effective approach and has great promise to determine the ecotoxicological effects and the underlying mechanisms of toxicity of heavy metals.

scholarly journals TOXIC EFFECTS OF LEAD ON FISH AND HUMAN

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
A Ishaque ◽  
S Ishaque ◽  
A Arif ◽  
HG Abbas
Keyword(s):  
Binding Capacity ◽  
Salt Water ◽  
Toxic Effects ◽  
Toxic Exposure ◽  
Accumulation Pattern ◽  
Antioxidant Responses ◽  
Oxidative Stresses ◽  
Exposure Pathway ◽  
Pb Accumulation ◽  
Pb Exposure

Bioaccumulation is a characteristic aspect in toxicity caused by Pb exposure. Toxic effects are induced in fish due to Pb exposure effecting its biochemical and physiological functions. Exposure pathway (dietary and waterborne), environmental factors (salt-water or fresh water) and Pb binding capacity with protein, SH and sulfur group decide accumulation pattern of Pb exposure. Activation of antioxidant responses like TBARS, GSH, GST, CAT and SOD occurs in fish for its protection in response to the oxidative stresses induced in fish due to Pb accumulation. Disruption of neurotransmitter function also occurs due to Pb accumulation which causes neurotoxicity in fish. Pb interaction also disturbs immune system responses. In fish, various systems are affected due to Pb toxic exposure which can be used as an indicator of toxicity in aquatic environment.

scholarly journals Human Body Burden of Heavy Metals and Health Consequences of Pb Exposure in Guiyu, an E-Waste Recycling Town in China

2021 ◽  
Vol 18 (23) ◽  
pp. 12428
Author(s):  
Wenlong Huang ◽  
Xiaoling Shi ◽  
Kusheng Wu
Keyword(s):  
Heavy Metals ◽  
Body Burden ◽  
Local Environment ◽  
Waste Recycling ◽  
The Body ◽  
Health Consequences ◽  
Long Term Effects ◽  
Adaptive Immune ◽  
Pb Exposure

Guiyu accommodates millions of tons of e-waste from overseas and domestic sources each year and is notorious for its e-waste dismantling industry. As a consequence, Guiyu has been described as “the world’s most toxic place” and “junk town”. Informal e-waste recycling activities have caused severe pollution to the local environment and are associated with extensive health problems to the residents. This review provides updated insights on the body burden of heavy metals derived from e-waste and health outcomes resulted from lead (Pb) exposure. The review identified that Guiyu has been highly contaminated by heavy metals, especially Pb. Excessive exposure to Pb has been associated with multi-system and long-term effects in neonates and children, covering nervous, cardiovascular, adaptive immune, and hematologic systems as well as chromosome and DNA damage. Our review indicates strong associations that emphasize the need to develop strong regulations for prevention of exposure and health consequences in Guiyu and similar sites around the world.

scholarly journals The Heavy Metals in Agrosystems and Impact on Health and Quality of Life

2015 ◽  
Vol 3 (2) ◽  
pp. 345-355 ◽  
Author(s):  
Adnan Tutic ◽  
Srecko Novakovic ◽  
Mitar Lutovac ◽  
Rade Biocanin ◽  
Sonja Ketin ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Animal Health ◽  
Toxic Effects ◽  
Basic Principles ◽  
Essential Trace Elements ◽  
Ionic Bonds ◽  
Negative Impacts ◽  
Surface And Groundwater ◽  
Quality Surface

The metal is a chemical element that conducts electricity well and heat, and the nonferrous metals builds cations and ionic bonds. Heavy metals include metals whose density is higher than 5 g/cm3. The whole range of the metal is in the form of essential trace elements, essential for a number of functions in the human body, and its deficiency results in a lack of occurrence of a serious symptom. The best examples are anemia lack of iron, lack of chromium in diabetes, growth problems in lack of nickel. Other elements such as lead, cadmium, mercury, arsenic and molybdenum have been shown to exhibit large quantities of toxic effects. The paper examines the problem of heavy metals originating from agriculture on agroecosystems. This group of pollutants is considered the most important cause of degradation of soil quality, surface and groundwater and direct causal adverse effects on human and animal health. In order to complete the environmental monitoring of pollutants, these main categories, origins, and possible negative impacts of the basic principles of preventing their toxic effects were examined.

Effect of metallothionein 1A rs8052394 polymorphism on lead, cadmium, zinc, and aluminum levels in factory workers

2020 ◽  
Vol 36 (10) ◽  
pp. 816-822
Author(s):  
Preeti Singh ◽  
Prasenjit Mitra ◽  
Taru Goyal ◽  
Pilla VSN Kiran Kumar ◽  
Shailja Sharma ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Metal Binding ◽  
Genetic Effect ◽  
Atomic Absorption Spectrophotometry ◽  
Toxic Effects ◽  
Nucleotide Polymorphisms ◽  
Factory Workers ◽  
Genotypic Distribution ◽  
Study Population ◽  
Hardy Weinberg Equilibrium

Metallothioneins (MTs) are low molecular weight cysteine-rich, metal-binding proteins. They are involved in transportation and detoxification of heavy metals, homeostasis of essential metals, and as antioxidation against reactive oxygen species. Polymorphisms in a gene may increase or decrease the expression efficiency of a gene. This study aimed to determine the genetic effect of MT1A rs8052394 on lead (Pb), cadmium (Cd), zinc (Zn), and aluminum (Al) levels in factory workers. The study included 100 occupationally heavy metal exposed workers from different factories around Jodhpur. Pb, Cd, Zn, and Al levels were measured by atomic absorption spectrophotometry. Individuals with the GG genotype had lower Pb, Zn, and Al levels and higher Cd levels than AA and AG genotypes. The genotyping of MT1A rs8052394 was done by the polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP). The mean ± standard deviation of Pb, Cd, Zn, and Al was 5.88 ± 13.28 µg/dL, 3.52 ± 1.25 µg/L, 16.45 ± 16.69 µg/dL, and 58.92 ± 58.91 µg/L, respectively. A significant association was found between single-nucleotide polymorphisms (SNPs) of MT1A gene and Cd ( p = 0.006) and with Zn levels ( p = 0.031) but no association found with Pb and Al levels. Among the study population, 78 participants were homozygote major (AA), 19 were heterozygote (AG), and 3 were homozygote minor (GG). The χ 2 test presented the genotypic distribution of all three genotypes under the Hardy–Weinberg equilibrium ( p > 0.05). The frequency of the A allele was 87.5% (175) and the G allele was 12.5% (25). To conclude, polymorphism in rs8052394 of the MT1A gene is associated with increased metal accumulation which in turn may lead to increased toxic effects in the exposed individuals. Factory workers thus, should be more cautious about protecting their health against the toxic effects of heavy metals.

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