147 Simplification of the follicle-stimulating hormone protocol for superovulation of the first follicular wave in sheep

2021 ◽  
Vol 33 (2) ◽  
pp. 181
Author(s):  
C. Brochado ◽  
B. J. Duran ◽  
J. C. L. Motta ◽  
J. D. Kieffer ◽  
A. Pinczak ◽  
...  
Keyword(s):  
Dose Group ◽  
Body Condition Score ◽  
Ovarian Response ◽  
Chorionic Gonadotrophin ◽  
P Value ◽  
Corpora Lutea ◽  
Embryo Production ◽  
Follicular Wave ◽  
Condition Score ◽  
Dose Number

The objective of the present study was to evaluate the effect of length of the FSH superstimulatory treatment on ovarian response and embryo production in sheep. Poll Dorset ewes (n=63) 3.2±0.2 years old weighing 58.5±1.5kg and with a body condition score of 2.7±0.1 (0=emaciated; 5=obese) were used during the transition from the breeding to the non-breeding season. All ewes received an ovarian superstimulatory treatment during the first follicular wave (Day 0 protocol). On Day −9 all ewes received a 0.3-g progesterone intravaginal device (CIDR, Zoetis) for 6 days. On Day −3, the CIDR was removed and all ewes were administered 125μg of cloprostenol sodium intramuscularly (IM) (PGF, estroPLAN, Parnell) and 200IU of equine chorionic gonadotrophin (eCG)+100IU of human chorionic gonadotrophin (hCG) IM (P.G. 600, Merck). Thirty-six hours after CIDR removal (Day −1.5) ewes were administered 100μg of gonadorelin acetate IM (gonadotrophin-releasing hormone, GnRH; Gonabreed, Parnell). Superstimulatory treatments were initiated on Day 0 (84 h after CIDR removal) with a total of 240mg of NIH-FSH-P1 (12 mL, Folltropin, Vetoquinol), and a new CIDR was used from the first to the last FSH dose (6-dose=3 days; 8-dose=4 days). Ewes were randomised to receive the total dose of FSH distributed in eight decreasing doses (8-dose group, n=33) or six decreasing doses (6-dose group, n=30) twice daily 12h apart. Two doses of PGF were administered with the last two doses of FSH, and GnRH was administered 12h after the last FSH administration. Intrauterine AI with fresh semen was performed by laparoscopy 16h after GnRH administration. Ovarian response was determined by laparoscopy 6 days after AI and ova/embryos were collected surgically in ewes with >2 corpora lutea (CL). Data were analysed using generalized linear mixed models (SAS 9.4; SAS Institute Inc.) and presented as mean±s.e.m. in Table 1. The percentage of responding donors (>2 CL) was 93.3% (28/30) and 78.8% (26/33; P=0.5) for the 6-dose and 8-dose groups, respectively. The number of CL tended (P=0.06) to be lesser in ewes in the 6-dose than the 8-dose group. However, no differences (P>0.10) were found in total ova/embryo, fertilized ova, transferable embryos, or freezable embryos. In conclusion, despite a tendency for a greater ovarian response in ewes treated with 8 doses of FSH, embryo yield did not appear to differ after either 6 or 8 doses of FSH administered during the first follicular wave in sheep. Table 1. Ovulatory response and embryo production in responding ewes (>2 corpora lutea) superstimulated with either 6 or 8 doses of FSH during the first follicular wave Outcome Treatment P-value 6-dose 8-dose Number of ewes 28 26 Corpora lutea (n) 14.2±1.7 20.3±2.8 0.06 Total ova/embryos (n) 9.0±1.3 10.9±1.8 0.38 Fertilized ova (n) 8.1±1.4 10.2±1.9 0.34 Fertilized ova (% per ewe) 89.0±5.0 81.7±7.0 0.40 Degenerate embryos 1.3±0.4 2.0±0.8 0.89 Transferable embryos (Grade 1–3) 6.8±1.2 8.2±1.8 0.52 Transferable embryos (% per ewe) 85.7±4.4 75.4±8.4 0.46 Freezable embryos (Grade 1–2) 6.6±1.1 8.2±1.8 0.43 Freezable embryos (% per ewe) 80.5±5.6 75.4±8.4 0.61

125 Influence of length of porcine follicle-stimulating hormone (p-FSH) treatment before ovum pickup on ovarian response and invitro embryo production in Holstein heifers

2021 ◽  
Vol 33 (2) ◽  
pp. 170
Author(s):  
J. C. L. Motta ◽  
R. V. Sala ◽  
V. A. Absalón-Medina ◽  
V. C. Fricke ◽  
M. Dominguez ◽  
...  
Keyword(s):  
Treatment Period ◽  
Recovery Rate ◽  
Body Condition Score ◽  
Ovarian Follicle ◽  
Ovarian Response ◽  
Medium Size ◽  
P Value ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Blastocyst Rate

Ovarian follicle stimulation with exogenous FSH before ovum pickup (OPU) in Bos taurus females is a common practice to increase invitro embryo production (IVP). The optimal stimulatory period length for OPU-IVP, however, has not been definitively ascertained. The objective of the present study, therefore, was to determine the effect of length of the superstimulatory treatment period before OPU on ovarian response and IVP in Holstein heifers. Nonpregnant heifers (n=57) 13.8±0.2 months of age with moderate body condition score (3.0±0.1; scale 1 to 5) were assigned in a completely randomised design to one of the following experimental groups: FSH2d: 200mg of p-FSH (Folltropin-V®, Vetoquinol) distributed in four injections (60, 60, 40, and 40mg) of FSH 12h apart; FSH3d: 200mg of p-FSH distributed in six injections (40, 40, 40, 40, 20, and 20mg) of FSH 12h apart. Timing of follicular wave emergence was synchronized by dominant follicle removal 36h before the first p-FSH injection in all heifers. An intravaginal progesterone (P4) implant (1.38g of P4 CIDR®, Zoetis) was inserted at the time of the first p-FSH injection and removed at the time of OPU, which occurred in all heifers at 44h (Nivet et al. 2012 Reproduction 143, 165-171; https://doi.org/10.1530/REP-11-0391) after the last p-FSH injection. Additionally, follicle number was determined at OPU and classified as small (<6mm), medium (6–10mm) or large (>10mm). Oocytes from follicles of different sizes were pooled by heifer at OPU and then classified and subjected to IVP procedures. Differences between treatment groups were evaluated using generalized linear mixed models (SAS 9.4; SAS Institute Inc.) and data are presented as mean±s.e.m. (Table 1). Lengthening the FSH treatment period resulted in a greater (P=0.01) number of large follicles; however, the number of small, medium-size, and total follicles was not different between groups. Number of total recovered oocytes, viable oocytes, cleaved oocytes, as well as recovery rate, percent viable oocytes, and cleavage rate were not different (P>0.2) between groups. Similarly, the number of blastocysts produced per heifer and blastocyst rate were not different (P>0.9) among groups. In conclusion, lengthening the period of FSH treatment by 1 day increased the number of large follicles at OPU but did not improve overall ovarian response, oocyte recovery, or embryo production. Table 1. Ovarian response and IVP of heifers treated with p-FSH during 2 or 3 days before ovum pickup Response FSH2d (n=28) FSH3d (n=29) P-value Small follicles (n) 5.9±0.6 5.7±0.8 0.83 Medium follicles (n) 17.0±2.4 12.9±1.6 0.18 Large follicles (n) 2.5±0.5 4.5±0.6 0.01 Total follicles (n) 25.4±2.6 23.1±1.8 0.60 Total oocytes (n) 17.0±2.5 13.0±1.2 0.23 Recovery rate (%) 62.6±3.7 56.9±3.1 0.26 Viable oocytes (n) 14.4±2.0 11.3±1.1 0.30 Viable oocytes (%) 85.0±2.4 88.0±3.0 0.31 Cleaved oocytes (n) 8.7±1.6 7.1±1.2 0.62 Cleavage rate (%) 54.7±5.7 54.1±5.7 0.96 Blastocysts (n) 3.2±0.6 3.2±0.7 0.98 Blastocyst rate (%) 20.6±4.0 22.4±3.7 0.97

113 Different doses of equine chorionic gonadotrophin on pregnancy and twin rates of Bos taurus beef heifers subjected to J-Synch protocol for fixed-time artificial insemination

2021 ◽  
Vol 33 (2) ◽  
pp. 164
Author(s):  
C. G. Pintos ◽  
R. Núñez-Olivera ◽  
F. Cuadro ◽  
C. Brochado ◽  
G. A. Bó ◽  
...  
Keyword(s):  
Bos Taurus ◽  
Body Condition Score ◽  
Fixed Time ◽  
Chorionic Gonadotrophin ◽  
P Value ◽  
Device Removal ◽  
Beef Heifers ◽  
Buserelin Acetate ◽  
Condition Score ◽  
Different Doses

The objective was to evaluate the effect of different doses of equine chorionic gonadotrophin (eCG) on pregnancy (P/AI) and twin rates of beef heifers in a progesterone and oestradiol-based treatment (J-Synch protocol) for fixed-time AI (FTAI). A total of 1,404 Bos taurus beef heifers with a body condition score 4.8±0.5 (mean±s.e.m., scale 1–8) were used in three different locations. The presence of a corpus luteum (CL) was determined by ovarian ultrasonography (5.0MHz) at the beginning of the J-Synch protocol. On Day 0, the heifers received 2mg of oestradiol benzoate (intramuscular, IM) associated with an intravaginal device containing 0.5g of progesterone. At device removal, on Day 6, 500µg of cloprostenol sodium was administered IM and the females were randomly assigned to one of two experimental groups to receive 200 (n=702) or 300 (n=702) IU of eCG (Zoetis) IM. Heifers were also tail-painted. On Day 0 females with paint rubbed-off were FTAI 64 h after device removal, and those that remained with the paint received 8 μg of buserelin acetate 64 h after device removal to be FTAI 8 h later. FTAI was performed at 64 to 72h after device removal, and pregnancy diagnosis was performed by ultrasonography 30 to 35 days after insemination. Data were analysed by logistic regression using GLMM. The results are shown in Table 1. Pregnancy rate was not different between heifers treated with 200 or 300IU of eCG (P=0.513) and was not affected by the presence of CL at the beginning of the protocol (P=0.513). Twin rate was greater in heifers treated with 300IU than with 200IU of eCG (P=0.005), and it was not affected by the presence of CL (P=0.362). Interaction between eCG treatment and presence of CL was not significant for pregnancy or twin rates (P=0.532). In conclusion, although 300IU of eCG increased the twin rate compared with 200IU, no differences were found in P/AI. We suggest that the dose of eCG in the J-Synch protocol could be adjusted to 200IU in Bos taurus beef heifers. Table 1. Pregnancy per AI (P/AI) and twin rates obtained with different doses of eCG (200 or 300IU) in a J-Synch protocol for fixed-time AI in cycling (CL) and anestrous (no CL) Bos taurus beef heifers Variable 200 IU 300 IU P-value CL no CL Total CL No CL Total eCG CL eCG×CL P/AI (%) 61.6% (135/219) 59.8% (289/483) 60.4% (424/702) 60.2% (133/221) 59.5% (286/481) 59.7% (419/702) 0.513 0.513 0.513 Twin rate (%) 3.0% (4/135) 3.5% (10/289) 3.3% (14/424) 6.8% (9/133) 9.4% (27/286) 8.6% (36/419) 0.005 0.362 0.532

71 Effect of follicle-stimulating hormone dose and circulating progesterone before ovum pickup and invitro embryo production in pregnant Holstein heifers

2021 ◽  
Vol 33 (2) ◽  
pp. 143
Author(s):  
C. Hayden ◽  
R. V. Sala ◽  
D. C. Pereira ◽  
L. C. Carrenho-Sala ◽  
M. Fosado ◽  
...  
Keyword(s):  
Body Condition Score ◽  
Medium Size ◽  
P Value ◽  
Limited Information ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Treatment Groups ◽  
Standard Procedures ◽  
Condition Score ◽  
Blastocyst Rate

Superstimulation with exogenous FSH before ovum pickup for invitro embryo production (IVP) improves embryo production. However, there is limited information on different FSH dose regimens and the effect of progesterone (P4) concentrations on IVP. Thus, the objective of the present study was to evaluate the effect of FSH dose and circulating P4 on ovarian superstimulation responses, before ovum pickup (OPU) on IVP in pregnant heifers. Pregnant Holstein heifers (n=47) 19.5±0.3 months of age with moderate body condition score (3.3±0.03; scale 1 to 5) were randomly assigned to 2 treatment groups, p-FSH (Folltropin-V, Vetoquinol) 160mg (FSH160) or p-FSH 300mg (FSH300), in a crossover design. Two sessions of OPU were performed on each animal at 48 and 62 days of gestation, with a washout interval between sessions of 14 days. Dominant follicle removal (DFR) was utilised to synchronize follicular wave emergence, and FSH treatments were initiated 36h later. Heifers in both groups received a total of 4 FSH injections 12h apart as follows: FSH160 (48.0, 42.7, 37.3, and 32.0mg) or FSH300 (90.0, 80.0, 70.0, and 60.0mg). All heifers underwent OPU 40h after the last FSH treatment. Blood samples were collected for P4 quantitation at OPU and number of small (<6mm), medium (6–10mm), and large (>10mm) follicles were determined before OPU. Cumulus–oocyte complexes (COCs) recovered were processed and invitro embryo production was performed using standard procedures. Differences between treatment groups were evaluated using generalized linear mixed models, and data are presented as mean±s.e.m. (Table 1). Heifers treated with 300mg of p-FSH had a greater (P<0.05) number of medium-size follicles, total number of follicles, total COCs recovered, number of viable COCs, and number of blastocysts produced. In addition, heifers treated with 300mg of p-FSH had a greater cleavage rate (P=0.03). Circulating P4 was on average 10.1±0.4ng mL−1 and did not differ between gestation days (P=0.12). Furthermore, circulating P4 did not affect (P>0.10) any of the outcomes evaluated, nor was there an FSH dose by circulating P4 interaction (P>0.05). In conclusion, increasing the dose of p-FSH before OPU from 160 to 300mg resulted in an increase in the total number of follicles, number of oocytes, and number of blastocysts produced in pregnant donors without changes in blastocyst rate, regardless of progesterone concentrations at the time of OPU. Table 1. Ovum pickup and invitro embryo production in pregnant heifers treated with different doses of p-FSH Item FSH160 FSH300 P-value Small follicles (n) 5.0±0.6 3.5±0.4 0.02 Medium follicles (n) 14.4±0.8 18.3±0.9 0.0003 Large follicles (n) 2.9±0.3 2.5±0.3 0.28 Total follicles (n) 22.3±0.7 24.3±0.9 0.04 Total oocytes (n) 12.8±0.7 14.7±1.0 0.02 Recovery rate (%) 56.2±2.1 59.7±2.7 0.20 Viable oocytes (n) 11.4±0.7 13.3±1.0 0.02 Viable oocytes (%) 89.9±1.7 88.0±2.4 0.22 Cleavage rate (%) 63.8±4.3 71.5±3.7 0.03 Cleaved oocytes (n) 7.2±0.7 9.3±0.9 0.14 Blastocysts (n) 2.7±0.4 3.6±0.7 0.02 Blastocyst rate (%) 22.0±3.3 22.4±3.6 0.74

The effect of time of mating on ovulation rate and potential lambing rate of Greyface ewes

1979 ◽  
Vol 29 (2) ◽  
pp. 277-282 ◽  
Author(s):  
R. G. Gunn ◽  
J. M. Doney ◽  
W. F. Smith
Keyword(s):  
Body Condition ◽  
Body Condition Score ◽  
Ovulation Rate ◽  
Stress Factors ◽  
Corpora Lutea ◽  
Differential Group ◽  
Condition Score ◽  
Group Feeding ◽  
Lambing Rate

ABSTRACTOver 2 years, 104 Greyface ewes were mated at a synchronized oestrus either in early October or early November. From late September until slaughter at return to service or between 4 and 7 weeks after mating, ewes were fed to maintain their body condition score as previously adjusted by differential group feeding on grass. Ovulation rate and potential lambing rate were derived from counts of corpora lutea and viable embryos at slaughter. Both rates were higher in the early-mated ewes in comparable body condition. Stress factors were implicated as contributing to the decline in ovulation rate with time, while ewes which failed to hold to first mating were largely responsible for the decline in potential lambing rate.

The effect of level of nutrition prior to mating on the reproductive performance of ewes of two welsh breeds in different levels of body condition

1991 ◽  
Vol 52 (1) ◽  
pp. 157-163 ◽  
Author(s):  
R. G. Gunn ◽  
T. J. Maxwell ◽  
D. A. Sim ◽  
J. R. Jones ◽  
M. E. James
Keyword(s):  
Body Condition ◽  
Reproductive Performance ◽  
Body Condition Score ◽  
High Surface ◽  
Live Weight ◽  
Corpora Lutea ◽  
Stocking Rate ◽  
Treatment Groups ◽  
Condition Score ◽  
Body Condition Scores

ABSTRACTThe effects of high-low (HL) and low-high (LH) patterns of nutrition during the 5 weeks prior to a synchronized mating were studied on the reproductive performance of 115 Welsh Mountain and 114 Brecknock Cheviot ewes in a range of body condition at the start of treatment. For 2 months prior to treatment imposition, ewes were grazed in a systematic way at different stocking rates on different sward heights to obtain a range of body condition scores. Target score groupings were 2·00 to 2·25, 2·50 to 2·75 and 3·00 to 3·25 and although scores of 1·50 to 3·25 were obtained, most lay in the 2·00 to 2·75 range. Two treatment groups, balanced for live weight and body condition score, received high and low levels of nutrition for 16 days from mid October, achieved, respectively, by a low stocking rate on a sward with a high surface height plus ad libitum concentrate and a high stocking rate on a sward with a low surface height plus 200 g hay per head per day. Treatments were then reversed for the 17 days prior to mating. Live-weight and body condition-score changes were recorded and reproductive performance at first mating was measured from counts of corpora lutea and viable embryos at slaughter either at return to service or at 4 weeks after first mating. There were no differences due to nutritional pattern in live weight or body condition score at mating. The LH treatment significantly increased the rates of ovulation, conception, multiple ova survival and potential lambing per ewe pregnant and per ewe mated in the Welsh Mountain breed but significantly increased only the rates of ovulation and potential lambing per ewe pregnant in the Brecknock Cheviot breed compared with the HL treatment. The relatively poorer response in the latter breed was partially due to a lower ovulation rate potential coupled with non-significantly lower rates of conception and survival of single-shed ova associated with the LH treatment. Much of this relatively poorer reproductive performance in the Brecknock Cheviot breed was in ewes with body condition scores 3= 2·75. Reproductive performance increased with increasing body condition at the start of treatment over the range of scores =≤ 2·25 to 2·50 in both breeds and, while continuing to improve in scores above 2·50 in the Welsh Mountain breed, in the Brecknock Cheviot breed it started to decline. Ewes with a body condition score of 2·50 in both breeds showed the greatest response in potential lambing rate to the LH treatment.

169 THE RELATIONSHIP BETWEEN OOCYTE RECOVERY AND EMBRYO PRODUCTION IN BOS INDICUS

2012 ◽  
Vol 24 (1) ◽  
pp. 196
Author(s):  
M. P. Palhão ◽  
E. R. Oliveira ◽  
M. M. Gioso ◽  
B. C. Carvalho ◽  
L. G. B. Siqueira ◽  
...  
Keyword(s):  
Body Condition Score ◽  
Bos Indicus ◽  
Pregnancy Rates ◽  
Embryo Production ◽  
Exact Test ◽  
Oocyte Recovery ◽  
Conversion Rates ◽  
Condition Score ◽  
The Relationship

The ovarian follicular population has been used as a parameter to evaluate fertility and also the potential of donors undergoing assisted reproductive procedures in both human medicine and animal practice. There is a high correlation between follicular population and oocyte recovery by ovum pickup (OPU), but the relationship between oocyte recovery, embryo production and pregnancy rates may not be fully understood. The aim of the present study was to evaluate whether the conversion rate of oocytes to embryos and further pregnancies could be positively related to the number of cumulus–oocyte complexes (COC) recovered after OPU in cattle. For this purpose, records of 626 OPU sections from 251 nonlactating Gyr cows (dairy Zebu breed) were analysed. The animals had a good body condition score, were kept in a good feeding pasture (Brachiaria spp.) and were supplemented with corn silage and a mixture of corn, soybeans and vitamin and minerals, according to their nutritional requirements. For each ovarian aspiration, the ovarian follicular wave was previously synchronized with an auricular implant (Norgestomet-Crestar®), IM injections of 2 mg of oestradiol benzoate (Gonadiol®) and 0.25 mg of D-cloprostenol (Sincrocio®). The OPU procedures were performed using an ultrasound device (Aquila Vet, Esaote, São Paulo, Brazil) equipped with a vaginal sector 7.5-MHz probe, disposable 20 G needles and a vacuum pressure of 80 mmHg. The cows were ranked in quartiles regarding the total number of COC recovered. To reduce bias related to the eventual fluctuation of OPU results, for the present analysis the authors used only the recorded OPU session of each cow with the highest number of COC recovered. Viable COC were fertilized with sex-sorted (X) semen of Gyr bulls previously tested for in vitro embryo production. Conversion rates (%) of the total and viable oocytes to embryos, viable oocytes to pregnancy and embryo to pregnancy were evaluated for each quartile. Differences between the first and fourth quartiles were accessed by Fisher's exact test. In the 251 OPU, 4246 total and 3173 viable COC were recovered, resulting in the production of 1001 embryos (31.5%) and 453 pregnancies (45.3%). The cows ranked in the first, second, third and fourth quartiles produced >30 (41.6 ± 10.6), 21 to 30 (25.2 ± 3.0), 12 to 20 (15.9 ± 2.6) and <12 (6.7 ± 3.1) total oocytes. The average viable oocyte (29.1 ± 11.0, 18.1 ± 5.3, 11.1 ± 3.7 and 4.5 ± 2.7, respectively) and embryo production (8.6 ± 5.7, 5.2 ± 3.6, 3.8 ± 2.8 and 1.8 ± 1.8, respectively) were different (P < 0.0001) among all quartiles. Pregnancy rates, however, did not differ (46.0, 44.9, 43.9 and 45.6%, respectively; P > 0.05). Interestingly, the conversion rates (viable oocytes to embryos and viable oocytes to pregnancies) were higher (P < 0.0001 and 0.002) in cows from the last quartile (51.1 and 31.9%) compared with those from the first quartile (23.7 and 14.7%). In conclusion, the number of COC recovered by OPU (and consequently the ovarian follicular count) can further predict the total number of embryos and pregnancies produced, but it is not directly related to the oocyte development potential. Biotran and Fapemig Project CVZ APQ 01654/09 and BPD 0007/10.

178 USE OF A NEW INTRAVAGINAL DEVICE TO RELEASE PROGESTERONE IN FIXED-TIME EMBRYO TRANSFER EMBRYO RECIPIENTS

2010 ◽  
Vol 22 (1) ◽  
pp. 247
Author(s):  
J. R. V. Pimentel ◽  
P. H. P. Miguez ◽  
A. Kehrle ◽  
E. H. Madureira ◽  
F. A. Braga ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Body Condition Score ◽  
Block Design ◽  
Fixed Time ◽  
Estradiol Benzoate ◽  
Sao Paulo ◽  
P Value ◽  
São Paulo ◽  
Condition Score ◽  
Intravaginal Device

Progestagen-releasing devices are commonly used for fixed-time artificial insemination and fixed-time embryo transfer (FTET). The aim of this study was to evaluate the efficacy of a new progesterone-releasing intravaginal device made of poly(3-hydroxy)butyrate-valerate copolymer (PHB-V) and poly-ϵ-caprolactone (PCL; Progestar®, Innovare, São Paulo, Brazil) to synchronize crossbredcows and heifers for FTET by determining ovulation, conception, and pregnancy rates. A total of 205 recipients ranging in age from 2 to 8 years (heifers, n = 77; non-suckled cows, n = 69; suckled cows, n = 59) were used on 3 different farms during February, March, and April 2008. The experimental design was a generalized block design (cyclicity, body condition score, postpartum interval, and animal category, i.e. heifers, suckled, or non-suckled cows), with 2 different synchronization treatments for FTET (Progestar® v. Crestar®). Cows were assigned to either Crestar® (norgestomet ear implants; Intervet Schering-Plough, Kenilworth, NJ, USA; n = 103) or Progestar® (PHB-V; n = 102) treatments. All groups were treated with 2 mg of estradiol benzoate i.m. (Innovare) and received the intravaginal or auricular device according to their group (Day 0). Together with P4 device withdrawal (Day 8), all animals received 400 IU of eCG i.m. (Novormon®, Schering-Plough) and 500 μg i.m. of PGF2α, (Sincrocio®, Ouro Fino, Brasil). On Day 9, all cows were treated with 1 mg of estradiol benzoate (Innovare) and the innovulation was performed on Day 17. Embryos were maintained at 37°C during the innovulation period. The percentage of usable recipients (AR) based on the presence of a functional CL on the day of embryo transfer, conception rate (numbers of pregnant recipients of the total number transferred; CR), and pregnancy rate (numbers of pregnant recipients of the total number synchronized; PR) were determined by ultrasonography 23 days after embryo transfer. Data were submitted to ANOVA (PROC GLM, SAS Institute Inc., Cary, NC, USA) and significant differences were considered to have a P-value of <0.05. None of the interactions were significant and there was no effect of treatment on AR (72.8% v. 83.3%), CR (47.9% v. 42.7%), and PR (34.7% v. 35.0%) for Progestar® v. Crestar® groups, respectively. This experiment showed that Progestar® was as efficacious as Crestar® to synchronize crossbred cows and heifers for FTET. FAPESP-Fundação de Amparo à Pesquisa do Estado de São Paulo and CNPq-Conselho Nacional de Desenvolvimento Científico e Tecnológico.

8 Evaluation of puberty induction protocol in peripubertal beef heifers prior to fixed-time AI programs

2019 ◽  
Vol 31 (1) ◽  
pp. 129
Author(s):  
J. B. S. Borges ◽  
D. S. V. Luiz ◽  
P. R. L. Aguiar ◽  
C. G. B. Berlitz ◽  
G. S. Velho ◽  
...  
Keyword(s):  
Breeding Season ◽  
Body Condition ◽  
Body Condition Score ◽  
Animal Health ◽  
Hormonal Treatment ◽  
Fixed Time ◽  
P Value ◽  
Ovarian Activity ◽  
Beef Heifers ◽  
Condition Score

Beef heifers that conceive early during their first breeding season calve earlier, wean heavier calves, and tend to continue doing so throughout the rest of their reproductive lives. Otherwise, under extensively managed systems in Southern Brazil, only a variable proportion of 2-year-old beef heifers reach puberty before the start of the breeding season. The aim of this study was to evaluate a protocol to induce puberty in peripubertal beef heifers using injectable progesterone (P4) and oestradiol cypionate (EC) to improve pregnancy per AI (P/AI) in fixed-time AI (FTAI) programs. A total of 311 Angus heifers, 24±3 months of age, 3.1±0.3 body condition score (1=emaciated; 5=obese), and 328±41kg of body weight (BW), were examined before FTAI protocol (Day −24) by transrectal ultrasonography to determine reproductive tract score (RTS; 1=prepubertal; 5=pubertal). Prepubertal heifers (RTS 1; 12.8%, 40/311) were excluded from the study. Heifers with RTS=2 (n=271) were assigned to 2 groups: P4 (RTS 2 or 3, n=100) and cyclic (RTS 4 and 5, n=171). Peripubertal heifers were treated on Day −24 with 150mg of injectable P4 IM (Sincrogest® injectable, Ourofino Animal Health, Cravinhos, Brazil) and on Day −12 received 1mg of EC IM (Sincro CP®, Ourofino Animal Health). Cyclic heifers received no hormonal treatment before FTAI protocol. On Day 0, heifers in the P4 group were evaluated again to determine RTS, body condition score, and BW. Induction of puberty was defined by the presence of a follicle &gt;8.5mm (RTS=4) or a corpus luteum (RTS=5). All heifers received an FTAI protocol consisting of 2mg of oestradiol benzoate IM (Sincrodiol®, Ourofino Animal Health) and an intravaginal progesterone implant (1 g; Sincrogest®, Ourofino Animal Health). On Day 8, heifers had implants removed and were injected with 1mg of EC and 500µg of sodium cloprostenol IM (Sincrocio®, Ourofino Animal Health). After 48h, all heifers received an FTAI. Thirty days after AI, the diagnosis of gestation was performed by ultrasonography. Statistics included chi-square and linear regression with coefficients analysed as independent variables, considering a significant P-value of 0.05. On Day 0, percentages of RTS 5, 4, and 3 in the P4 heifers were 37, 27, and 36%, respectively, resulting in 64% of puberty induction. The P/AI was similar (P=0.14) in P4-treated and cyclic heifers (59v. 50.3%, respectively). The regression analysis presented a linear and positive effect of BW on P/AI (P&lt;0.001) in cyclic heifers and a positive influence of BW on RTS (P=0.05) and RTS on P/AI (P=0.008) in P4-treated heifers. The application of RTS system associated with P4 treatment of peripubertal heifers before FTAI protocol improved ovarian activity and might have contributed to higher P/AI, comparable with that achieved in cyclic heifers.

Effect of Body Condition Score (BCS) on In Vivo Embryo Production and Pregnancy Rate Following Superovulation in Hanwoo

2013 ◽  
Vol 28 (1) ◽  
pp. 7-12
Author(s):  
Gyu-Tae Yeom ◽  
◽  
Hae-Geum Park ◽  
Nam-Tae Kim ◽  
Sung-Woo Kim ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Body Condition ◽  
Body Condition Score ◽  
Embryo Production ◽  
Condition Score

418 EFFECT OF SUPEROVULATION INITIATED AT FOLLICULAR WAVE EMERGENCE IN Santa Inês EWES

2010 ◽  
Vol 22 (1) ◽  
pp. 366
Author(s):  
B. D. M. Silva ◽  
T. A. S. N. Silva ◽  
L. Dalcin ◽  
C. M. Lucci ◽  
J. P. Neves
Keyword(s):  
Total Dose ◽  
Buenos Aires ◽  
Ovarian Response ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Treatment Groups ◽  
Follicular Wave ◽  
Preliminary Study ◽  
Santa Inês ◽  
Fresh Semen

Superovulation in ewes has been a source of many studies aimed at obtaining high superovulatory response and number of viable embryos recovered. In a protocol called Day 0, superovulatory treatment was initiated at the time of wave emergence in the absence of a dominant follicle (Menchaca A et al. 2002 Theriogenology 58, 1713-1721). The aim of this study was to compare ovarian response and number of embryos recovered after treatment between ewes treated with a Day 0 protocol and those treated with a traditional protocol. Santa InÊs ewes (n= 18) between 2 and 5 years old were randomly distributed into 2 superovulation treatment groups: traditional protocol and Day 0 protocol. Each treatment was repeated twice in a crossover model. The traditional protocol consisted of the intravaginal insertion of a sponge containing 60 mg of medroxiprogesterone acetate (MAP; Progespon®, Tecnopec, São Paulo, Brazil) for 14 days, which was replaced on Day 7, followed by 150 μg of cloprostenol (Prolise®, Tecnopec) i.m. On Day 12, FSH (Folltropin®-V, Bioniche, Belleville, Ontario, Canada) treatment was initiated using a total dose of 200 mg, given in twice-daily i.m. injections that decreased in dose over 4 days. A dose of 200 IU of eCG (Novormon®, Syntex, Buenos Aires, Argentina) i.m. was given at the time of sponge withdrawal. The AI was done by laparoscopy at 48 and 55 h after sponge withdrawal using fresh semen. The Day 0 protocol consisted of a previous 9-day synchronization treatment with a sponge containing 60 mg of MAP, followed by 150 μ of cloprostenol i.m. and 200 IU of eCG i.m. given on Day 7. A dose of 0.05 mg of GnRH (lecirelin; Gestran Plus®, Tecnopec) i.m. was given 16 h after sponge withdrawal. In a preliminary study, 38 ewes ovulated 42 ± 52 h after sponge withdrawal. Therefore, 48 h after sponge removal was considered as Day 0 and FSH treatment was initiated at that time, with a total dose of 200 mg of Folltropin®-V, given in 6 twice-daily decreasing doses. Two doses of cloprostenol (150 μg) were given i.m. concurrent with the fifth and sixth FSH treatments. Gonadotropin-releasing hormone (0.05 mg of lecirelin i.m.) was given 12 h after the last FSH treatment. Artificial insemination with fresh semen was done by laparoscopy 16 and 26 h after GnRH treatment. Five days after AI, embryos were recovered surgically. Results were evaluated by the parametric t-test. The number of corpora lutea and ova/embryos recovered did not differ (P > 0.05) between the traditional (9.8 ± 5.3; 4.5 ± 4.6) and Day 0 (10.0 ± 6.0; 3.5 ± 4.3) protocols. Similarly, no difference in the number of viable embryos was observed between treatments (1.6 ± 2.0 and 1.7 ± 2.4 for the traditional and Day 0 treatments, respectively). Within viable embryos, the traditional protocol (0.4 ± 1.0) resulted in ahigher (P < 0.05) number of morulae than that of the Day 0 protocol (0.1 ± 0.3). The ewes that had no superovulatory response did not differ (P > 0.05) between the traditional (11.11%) and Day 0 (5.56%) protocols. In summary, there was no difference in the parameters evaluated between both protocols. Financial support: FAPDF, CNPq, Tecnopec.

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