171 Blood sample type and storage conditions affect circulating anti-Müllerian hormone concentrations in cattle

2022 ◽  
Vol 34 (2) ◽  
pp. 324
Author(s):  
D. I. Saade-Rampolla ◽  
J. C. L. Motta ◽  
A. E. Crist ◽  
A. Garcia-Guerra
Keyword(s):  
Blood Sample ◽  
Storage Conditions ◽  
Sample Type ◽  
And Storage

scholarly journals Sample Type and Storage Conditions Affect Calprotectin Measurements in Blood

2017 ◽  
Vol 2 (6) ◽  
pp. 851-856 ◽  
Author(s):  
Lise Pedersen ◽  
Erling Birkemose ◽  
Charlotte Gils ◽  
Sara Safi ◽  
Mads Nybo
Keyword(s):  
Storage Conditions ◽  
Sample Type ◽  
And Storage

scholarly journals Effect of sample type, centrifugation and storage conditions on vitamin D concentration

2013 ◽  
pp. 321-325 ◽  
Author(s):  
Ayfer Colak ◽  
Burak Toprak ◽  
Nese Dogan ◽  
Fusun Ustuner
Keyword(s):  
Vitamin D ◽  
Storage Conditions ◽  
Sample Type ◽  
And Storage

scholarly journals Influence of Sample Type and Storage Conditions on Soluble CD40 Ligand Assessment

2006 ◽  
Vol 52 (5) ◽  
pp. 888-891 ◽  
Author(s):  
Michael Weber ◽  
Birgitt Rabenau ◽  
Michael Stanisch ◽  
Albrecht Elsaesser ◽  
Vesselin Mitrovic ◽  
...  
Keyword(s):  
Cd40 Ligand ◽  
Storage Conditions ◽  
Sample Type ◽  
Serum Samples ◽  
Soluble Cd40 Ligand ◽  
Coronary Syndromes ◽  
Edta Plasma ◽  
Few Data ◽  
The Impact ◽  
And Storage

Abstract Background: Several studies have consistently shown that soluble CD40 ligand (sCD40L) concentrations are increased in patients with acute coronary syndromes and can serve as a biomarker for risk stratification. However, few data are available on preanalytic conditions that impact sCD40L values. Thus, the aim of our prospective study was to evaluate the impact of sampling techniques and storage conditions on sCD40L concentrations. Methods: We included a total of 30 patients with no, stable, or unstable coronary heart disease. Blood samples were collected in gel-filled tubes without additives, in EDTA-filled tubes, and in citrate-filled tubes and were kept at various storage conditions. Results: Median (interquartile range) sCD40L values at baseline were higher in serum samples [5.29 (3.89–6.33) μg/L] than in either EDTA plasma [0.78 (0.39–1.12) μg/L; P <0.001] or citrate plasma [0.37 (0.22–0.51) μg/L; P <0.001]. Serum values increased with delayed processing [7.94 (5.97–9.62) μg/L after 1.5 h (P <0.001) vs baseline; 10.55 (7.58–11.55) μg/L after 3 h (P <0.001) vs baseline]. However, after centrifugation, sCD40L values remained stable for all 3 sample types. Conclusion: Plasma, but not serum, samples are appropriate for sCD40L measurements. In general, preanalytic conditions are critical in the assessment of sCD40L concentrations and thus should be carefully considered for future studies.

Barley seed storage under controlled conditions

2019 ◽  
pp. 140-150 ◽  
Author(s):  
O. A. Zadorozhna ◽  
T. P. Shyianova ◽  
M.Yu. Skorokhodov
Keyword(s):  
Hordeum Vulgare ◽  
Moisture Content ◽  
Spring Barley ◽  
Seed Storage ◽  
Growing Season ◽  
Storage Conditions ◽  
Meteorological Conditions ◽  
Hordeum Vulgare L ◽  
The Relationship ◽  
And Storage

Seed longevity of 76 spring barley gene pool samples (Hordeum vulgare L. subsp. distichon, convar. distichon: 56 nutans Schubl., two deficience (Steud.) Koern., two erectum Rode ex Shuebl., two medicum Koern.; convar. nudum (L.) A.Trof.: one nudum L. та subsp. vulgare: convar. vulgare: nine pallidum Ser., three rikotense Regel.; convar. coeleste (L.) A.Trof.: one coeleste (L.) A.Trof.) from 26 countries, 11 years and four places of reproduction was analyzed. Seeds with 5–8% moisture content were stored in chamber with unregulated and 4oC temperature. The possibility of seed storage under these conditions for at least 10 years without significant changes in germination has been established. The importance of meteorological conditions in the formation and ripening of seeds for their longevity is confirmed. The relationship between the decrease of barley seeds longevity and storage conditions, amount of rainfall, temperature regime during the growing season of plants is discussed.

Comparison of Procedures for Isolation of Monoterpene Hydrocarbons from Fresh Needles of Picea abies and Picea omorica

2000 ◽  
Vol 65 (7) ◽  
pp. 1073-1081 ◽  
Author(s):  
Valerie Holubová ◽  
Iva Chvílíčková ◽  
Vlastimil Kubáň
Keyword(s):  
Picea Abies ◽  
Liquid Nitrogen ◽  
Steam Distillation ◽  
Extraction Efficiency ◽  
Total Content ◽  
Storage Conditions ◽  
Fresh Weight ◽  
Hexane Extraction ◽  
Monoterpene Hydrocarbons ◽  
And Storage

Extraction procedures (steam distillation, supercritical fluid extraction and solvent extraction) for isolation of monoterpene hydrocarbons from fresh needles of Picea abies and Picea omorica were optimised. The procedures were compared with the aim of minimizing consumption of needles and improving the extraction efficiency and repeatability. An influence of homogenisation procedures and storage conditions (liquid nitrogen, -18 and 4 °C) on the total content and composition of essential oils was studied. Cryogenic grinding (liquid nitrogen) combined with the extraction with cold hexane (extraction time 2 h) and subsequent GC-MS determination in freshly homogenised needles gives the best results (1.5-4 times better extraction efficiency, RSD < 10% for P. abies and < 25% for P. omorica). Limits of detections (3 S/N) for individual monoterpene hydrocarbons from units to tens of ng/g and recoveries 97.2-101.4% were found in fresh needles (calculated to fresh weight). While cooling to 4 °C is unacceptable, freezing at -18 °C for the period of 18 days in the dark gives also good results.

scholarly journals Changes of Physicochemical Properties of Starch Syrups Recommended for Winter Feeding of Honeybees during Storage

Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 374
Author(s):  
Teresa Szczęsna ◽  
Ewa Waś ◽  
Piotr Semkiw ◽  
Piotr Skubida ◽  
Katarzyna Jaśkiewicz ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Physicochemical Properties ◽  
Physicochemical Parameters ◽  
Storage Temperature ◽  
Storage Conditions ◽  
Different Temperatures ◽  
And Storage ◽  
Bee Colonies ◽  
Temperature Time ◽  
Winter Feeding

The aim of this study was to determine the influence of storage temperature and time on physicochemical parameters of starch syrups recommended for the winter feeding of bee colonies. The studies included commercially available three starch syrups and an inverted saccharose syrup that were stored at different temperatures: ca. 20 °C, 10–14 °C, and ca. 4 °C. Physicochemical parameters of fresh syrups (immediately after purchase) and syrups after 3, 6, 9, 12, 15, 18, 21, and 24 months of storage at the abovementioned temperatures were measured. It was observed that the rate of unfavorable changes in chemical composition of starch syrups and the inverted saccharose syrup, mainly the changes in the 5-hydroxymethylfurfural (HMF) content, depended on the type of a syrup and storage conditions (temperature, time). Properties of tested starch syrups intended for winter feeding of bees stored at ca. 20 °C maintained unchanged for up to 6 months, whereas the same syrups stored at lower temperatures (10–14 °C) maintained unchanged physicochemical parameters for about 12 months. In higher temperatures, the HMF content increased. To date, the influence of this compound on bees has not been thoroughly investigated.

scholarly journals Stabilizing Grain Yield and Nutrition Quality in Purple Rice Varieties by Management of Planting Elevation and Storage Conditions

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 83
Author(s):  
Supapohn Yamuangmorn ◽  
Suchada Jumrus ◽  
Sansanee Jamjod ◽  
Narit Yimyam ◽  
Chanakan Prom-u-Thai
Keyword(s):  
Grain Yield ◽  
Antioxidant Capacity ◽  
Nutritional Value ◽  
Brown Rice ◽  
Storage Conditions ◽  
Harvest Management ◽  
Rice Varieties ◽  
Opposite Pattern ◽  
Dpph Activity ◽  
And Storage

Purple rice has become an interesting source of nutritional value among healthy cereal grains. The appropriate cultivation together with post-harvest management would directly benefit farmers and consumers. This study aimed (i) to determine the yield, grain nutritional quality, and antioxidant capacity of purple rice varieties grown at lowland and highland elevations, and (ii) to evaluate the effects of storage conditions on the stability of the rice nutritional value during six months of storage. The high anthocyanin PES variety grown in the lowlands had a higher grain yield than the plants grown in the highlands, but grain anthocyanin concentration had the opposite pattern. In the high antioxidant capacity KAK variety, grain yield and DPPH activity were not significantly different between plants grown at the two elevations. The storage of brown rice and vacuum-sealed packages were both found to preserve greater anthocyanin concentrations in PES, but there was no effect on the DPPH activity of KAK. The grain properties were not significantly different between storage at 4 °C and room temperature. This study suggests that the optimal cultivation practices and storage conditions would result in the higher yield and grain quality of purple rice varieties.

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