scholarly journals Biologic-free mechanically induced muscle regeneration

2016 ◽  
Vol 113 (6) ◽  
pp. 1534-1539 ◽  
Author(s):  
Christine A. Cezar ◽  
Ellen T. Roche ◽  
Herman H. Vandenburgh ◽  
Georg N. Duda ◽  
Conor J. Walsh ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Muscle Injury ◽  
Contractile Force ◽  
Functional Restoration ◽  
Material System ◽  
Magnetic Actuation ◽  
Loss Of Function ◽  
Muscle Injuries ◽  
Threefold Increase

Severe skeletal muscle injuries are common and can lead to extensive fibrosis, scarring, and loss of function. Clinically, no therapeutic intervention exists that allows for a full functional restoration. As a result, both drug and cellular therapies are being widely investigated for treatment of muscle injury. Because muscle is known to respond to mechanical loading, we investigated instead whether a material system capable of massage-like compressions could promote regeneration. Magnetic actuation of biphasic ferrogel scaffolds implanted at the site of muscle injury resulted in uniform cyclic compressions that led to reduced fibrous capsule formation around the implant, as well as reduced fibrosis and inflammation in the injured muscle. In contrast, no significant effect of ferrogel actuation on muscle vascularization or perfusion was found. Strikingly, ferrogel-driven mechanical compressions led to enhanced muscle regeneration and a ∼threefold increase in maximum contractile force of the treated muscle at 2 wk compared with no-treatment controls. Although this study focuses on the repair of severely injured skeletal muscle, magnetically stimulated bioagent-free ferrogels may find broad utility in the field of regenerative medicine.

scholarly journals Recent Trends in Injury Models to Study Skeletal Muscle Regeneration and Repair

2020 ◽  
Vol 7 (3) ◽  
pp. 76 ◽  
Author(s):  
Sydnee T. Sicherer ◽  
Rashmi S. Venkatarama ◽  
Jonathan M. Grasman
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Muscle Regeneration ◽  
Muscle Injury ◽  
Large Scale ◽  
Skeletal Muscle Regeneration ◽  
Muscle Injuries ◽  
Injury And Repair ◽  
Injury Models

Skeletal muscle injuries that occur from traumatic incidents, such as those caused by car accidents or surgical resections, or from injuries sustained on the battlefield, result in the loss of functionality of the injured muscle. To understand skeletal muscle regeneration and to better treat these large scale injuries, termed volumetric muscle loss (VML), in vivo injury models exploring the innate mechanisms of muscle injury and repair are essential for the creation of clinically applicable treatments. While the end result of a muscle injury is often the destruction of muscle tissue, the manner in which these injuries are induced as well as the response from the innate repair mechanisms found in muscle in each animal models can vary. This targeted review describes injury models that assess both skeletal muscle regeneration (i.e., the response of muscle to myotoxin or ischemic injury) and skeletal muscle repair (i.e., VML injury). We aimed to summarize the injury models used in the field of skeletal muscle tissue engineering, paying particular attention to strategies to induce muscle damage and how to standardize injury conditions for future experiments.

Antifibrotic effects of suramin in injured skeletal muscle after laceration

2003 ◽  
Vol 95 (2) ◽  
pp. 771-780 ◽  
Author(s):  
Yi-Sheng Chan ◽  
Yong Li ◽  
William Foster ◽  
Takashi Horaguchi ◽  
George Somogyi ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Growth Factor ◽  
Muscle Regeneration ◽  
Sports Medicine ◽  
Transforming Growth Factor ◽  
Smooth Muscle Actin ◽  
Healing Process ◽  
Muscle Injuries

Muscle injuries are very common in traumatology and sports medicine. Although muscle tissue can regenerate postinjury, the healing process is slow and often incomplete; complete recovery after skeletal muscle injury is hindered by fibrosis. Our studies have shown that decreased fibrosis could improve muscle healing. Suramin has been found to inhibit transforming growth factor (TGF)-β1 expression by competitively binding to the growth factor receptor. We conducted a series of tests to determine the antifibrotic effects of suramin on muscle laceration injuries. Our results demonstrate that suramin (50 μg/ml) can effectively decrease fibroblast proliferation and fibrotic-protein expression (α-smooth muscle actin) in vitro. In vivo, direct injection of suramin (2.5 mg) into injured murine muscle resulted in effective inhibition of muscle fibrosis and enhanced muscle regeneration, which led to efficient functional muscle recovery. These results support our hypothesis that prevention of fibrosis could enhance muscle regeneration, thereby facilitating more efficient muscle healing. This study could significantly contribute to the development of strategies to promote efficient muscle healing and functional recovery.

scholarly journals MiR-27b Promotes Muscle Development by Inhibiting MDFI Expression

2018 ◽  
Vol 46 (6) ◽  
pp. 2271-2283 ◽  
Author(s):  
Lianjie Hou ◽  
Jian Xu ◽  
Yiren Jiao ◽  
Huaqin Li ◽  
Zhicheng Pan ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Western Blot ◽  
Satellite Cells ◽  
Muscle Regeneration ◽  
Muscle Injury ◽  
Target Gene ◽  
Muscle Development ◽  
Muscle Satellite Cells ◽  
Qrt Pcr

Background/Aims: Skeletal muscle plays an essential role in the body movement. However, injuries to the skeletal muscle are common. Lifelong maintenance of skeletal muscle function largely depends on preserving the regenerative capacity of muscle. Muscle satellite cells proliferation, differentiation, and myoblast fusion play an important role in muscle regeneration after injury. Therefore, understanding of the mechanisms associated with muscle development during muscle regeneration is essential for devising the alternative treatments for muscle injury in the future. Methods: Edu staining, qRT-PCR and western blot were used to evaluate the miR-27b effects on pig muscle satellite cells (PSCs) proliferation and differentiation in vitro. Then, we used bioinformatics analysis and dual-luciferase reporter assay to predict and confirm the miR-27b target gene. Finally, we elucidate the target gene function on muscle development in vitro and in vivo through Edu staining, qRT-PCR, western blot, H&E staining and morphological observation. Result: miR-27b inhibits PSCs proliferation and promotes PSCs differentiation. And the miR-27b target gene, MDFI, promotes PSCs proliferation and inhibits PSCs differentiation in vitro. Furthermore, interfering MDFI expression promotes mice muscle regeneration after injury. Conclusion: our results conclude that miR-27b promotes PSCs myogenesis by targeting MDFI. These results expand our understanding of muscle development mechanism in which miRNAs and genes work collaboratively in regulating skeletal muscle development. Furthermore, this finding has implications for obtaining the alternative treatments for patients with the muscle injury.

Platelet-Rich Plasma Enhances Equine Skeletal Muscle Regeneration in a Bupivacaine-Induced Muscle Injury Model

2021 ◽  
Author(s):  
Kentaro Fukuda ◽  
Taisuke Kuroda ◽  
Norihisa Tamura ◽  
Hiroshi Mita ◽  
Hirofumi Miyata ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Regeneration ◽  
Muscle Injury ◽  
Platelet Rich Plasma ◽  
Skeletal Muscle Regeneration ◽  
Injury Model

scholarly journals A Histoarchitectural Approach to Skeletal Muscle Injury: Searching for a Common Nomenclature

2020 ◽  
Vol 8 (3) ◽  
pp. 232596712090909 ◽  
Author(s):  
◽  
Ramon Balius ◽  
Marc Blasi ◽  
Carles Pedret ◽  
Xavier Alomar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Connective Tissue ◽  
Muscle Injury ◽  
Muscle Injuries ◽  
Macroscopic Anatomy ◽  
Skeletal Muscle Injury ◽  
Tissue Structures ◽  
Definition Of

In recent years, different classifications for muscle injuries have been proposed based on the topographic location of the injury within the bone-tendon-muscle chain. We hereby propose that in addition to the topographic classification of muscle injuries, a histoarchitectonic (description of the damage to connective tissue structures) definition of the injury be included within the nomenclature. Thus, the nomenclature should focus not only on the macroscopic anatomy but also on the histoarchitectonic features of the injury.

scholarly journals An Old Problem: Aging and Skeletal-Muscle-Strain Injury

2017 ◽  
Vol 26 (2) ◽  
pp. 180-188 ◽  
Author(s):  
Brent A. Baker
Keyword(s):  
Skeletal Muscle ◽  
Clinical Relevance ◽  
Muscle Injury ◽  
Functional Restoration ◽  
Clinical Scenario ◽  
Muscle Strain ◽  
Tissue Quality ◽  
Organ Systems ◽  
Muscle Strain Injury

Clinical Scenario:Even though chronological aging is an inevitable phenomenological consequence occurring in every living organism, it is biological aging that may be the most significant factor challenging our quality of life. Development of functional limitations, resulting from improper maintenance and restoration of various organ systems, ultimately leads to reduced health and independence. Skeletal muscle is an organ system that, when challenged, is often injured in response to varying stimuli. Overt muscle-strain injury can be traumatic, clinically diagnosable, properly managed, and a remarkably common event, yet our contemporary understanding of how age and environmental stressors affect the initial and subsequent induction of injury and how the biological processes resulting from this event are modifiable and, eventually, lead to functional restoration and healing of skeletal muscle and adjacent tissues is presently unclear. Even though the secondary injury response to and recovery from "contraction-induced" skeletal-muscle injury are impaired with aging, there is no scientific consensus as to the exact mechanism responsible for this event. Given the multitude of investigative approaches, particular consideration given to the appropriateness of the muscle-injury model, or research paradigm, is critical so that outcomes may be physiologically relevant and translational. In this case, methods implementing stretch-shortening contractions, the most common form of muscle movements used by all mammals during physical movement, work, and activity, are highlighted.Clinical Relevance:Understanding the fundamental evidence regarding how aging influences the responsivity of skeletal muscle to strain injury is vital for informing how clinicians approach and implement preventive strategies, as well as therapeutic interventions. From a practical perspective, maintaining or improving the overall health and tissue quality of skeletal muscle as one ages will positively affect skeletal muscle’s safety threshold and responsivity, which may reduce incidence of injury, improve recovery time, and lessen overall fiscal burdens.

Enhancement of satellite cell differentiation and functional recovery in injured skeletal muscle by hyperbaric oxygen treatment

2014 ◽  
Vol 116 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Masaki Horie ◽  
Mitsuhiro Enomoto ◽  
Manabu Shimoda ◽  
Atsushi Okawa ◽  
Shumpei Miyakawa ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Satellite Cell ◽  
Hyperbaric Oxygen ◽  
Functional Recovery ◽  
Muscle Regeneration ◽  
Muscle Injury ◽  
Muscle Fibers ◽  
Maximum Force ◽  
Pcr Analysis ◽  
Regulatory Factor

Recently, the use of hyperbaric oxygen (HBO) treatments by elite athletes to accelerate recovery from muscle injuries has become increasingly popular. However, the mechanism of promoting muscle regeneration under HBO conditions has not yet been defined. In this study, we investigated whether HBO treatments promoted muscle regeneration and modulated muscle regulatory factor expression in a rat skeletal muscle injury model. Muscle injury was induced by injecting cardiotoxin (CTX) into the tibialis anterior (TA) muscles. As the HBO treatment, rats were placed in an animal chamber with 100% oxygen under 2.5 atmospheres absolute for 2 h/day, 5 days/wk for 2 wk. We then performed histological analyses, measured the maximum force-producing capacity of the regenerating muscle fibers, and performed quantitative RT-PCR analysis of muscle regulatory factor mRNAs. The cross-sectional areas and maximum force-producing capacity of the regenerating muscle fibers were increased by HBO treatment after injury. The mRNA expression of MyoD, myogenin, and IGF-1 increased significantly in the HBO group at 3 and 5 days after injury. The number of Pax7+/MyoD+, Pax7−/MyoD+, and Pax7+/BrdU+-positive nuclei was increased by HBO treatment. In this study, we demonstrated that HBO treatment accelerated satellite cell proliferation and myofiber maturation in rat muscle that was injured by a CTX injection. These results suggest that HBO treatment accelerates healing and functional recovery after muscle injury.

scholarly journals Tissue Engineered Strategies for Skeletal Muscle Injury

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Umile Giuseppe Longo ◽  
Mattia Loppini ◽  
Alessandra Berton ◽  
Filippo Spiezia ◽  
Nicola Maffulli ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Muscle Injury ◽  
Platelet Rich Plasma ◽  
Residual Effects ◽  
Current Therapy ◽  
Muscle Injuries ◽  
Cell Based Therapy ◽  
Proliferation And Differentiation ◽  
Inflammatory Drugs

Skeletal muscle injuries are common in athletes, occurring with direct and indirect mechanisms and marked residual effects, such as severe long-term pain and physical disability. Current therapy consists of conservative management including RICE protocol (rest, ice, compression, and elevation), nonsteroidal anti-inflammatory drugs, and intramuscular corticosteroids. However, current management of muscle injuries often does not provide optimal restoration to preinjury status. New biological therapies, such as injection of platelet-rich plasma and stem-cell-based therapy, are appealing. Although some studies support PRP application in muscle-injury management, reasons for concern persist, and further research is required for a standardized and safe use of PRP in clinical practice. The role of stem cells needs to be confirmed, as studies are still limited and inconsistent. Further research is needed to identify mechanisms involved in muscle regeneration and in survival, proliferation, and differentiation of stem cells.

scholarly journals Applications of Small Molecules in Muscle Tissue Engineering

2021 ◽  
Author(s):  
Behnaz Mirza Ahmadi ◽  
Mahmood Talkhabi ◽  
Sarah Rajabi
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Small Molecules ◽  
Muscle Tissue ◽  
Muscle Regeneration ◽  
Cost Effective ◽  
Clinical Approach ◽  
Muscle Injuries ◽  
Inducing Factors

Introduction: Skeletal muscles account for about 40% of the total body weight. Every year, hundreds of people lose at least part of their muscle tissue due to illness, war, and accidents. This can lead to disruption of activities such as breathing, movement, and social life. To this end, various therapeutic strategies such as medication therapy, cell therapy and tissue transplantation have been used or studied in muscle regeneration. However, there is no effective and well-defined clinical approach for treatment of muscle injuries and the severity of muscle injuries increase with age in most cases. Therefore, investigation for finding new and effective clinical approach for muscle regeneration is one of the most important issues in basic and clinical researches. Tissue engineering is considered as one of the promising and newest approach for skeletal muscle tissue regeneration and provides an appropriate model for personalized medicine and basic researches that can be used in personalized medicine and basic research. Besides biomaterials and cells, inducing factors are another element of tissue engineering. These factors influence epigenetic mechanisms and signaling pathway, thereby inducing proliferation, differentiation, and migration of cells used in muscle tissue engineering, and accelerates muscle formation in vitro. Recently, small molecules have been used as alternatives to growth factors or along with other inducing factors in muscle tissue engineering. Since they do not induce an immune reaction, penetrate easily to the cells and have a specific molecular target, therefore they have attracted much attention as the cost-effective inducing factors in tissue engineering. Conclusion:  Taken together, the effective small molecules in muscle tissue engineering can be used with different biomaterial conditions (e.g. hydrogel, decellularized tissue, and synthetic scaffolds) in both in vivo and in vitro, resulting to production of cost effective and highly efficient engineered muscle tissues that help to achieve therapeutical goals of muscle tissue engineering. Herein, we describe tissue engineering and review the small molecules used in skeletal muscle tissue engineering.

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