scholarly journals Coordinated regulation of vegetative and reproductive branching in rice

2015 ◽  
Vol 112 (50) ◽  
pp. 15504-15509 ◽  
Author(s):  
Lei Wang ◽  
Shengyuan Sun ◽  
Jiye Jin ◽  
Debao Fu ◽  
Xuefei Yang ◽  
...  
Keyword(s):  
Grain Yield ◽  
Genetic Improvement ◽  
Gene Network ◽  
Inflorescence Meristem ◽  
Squamosa Promoter Binding Protein ◽  
Spl Genes ◽  
Branching Patterns ◽  
Related Proteins ◽  
Rice Genetic ◽  
Fine Tune

Grasses produce tiller and panicle branching at vegetative and reproductive stages; the branching patterns largely define the diversity of grasses and constitute a major determinant for grain yield of many cereals. Here we show that a spatiotemporally coordinated gene network consisting of the MicroRNA 156 (miR156/)miR529/SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) and miR172/APETALA2 (AP2) pathways regulates tiller and panicle branching in rice. SPL genes negatively control tillering, but positively regulate inflorescence meristem and spikelet transition. Underproduction or overproduction of SPLs reduces panicle branching, but by distinct mechanisms: miR156 and miR529 fine-tune the SPL levels for optimal panicle size. miR172 regulates spikelet transition by targeting AP2-like genes, which does not affect tillering, and the AP2-like proteins play the roles by interacting with TOPLESS-related proteins (TPRs). SPLs modulate panicle branching by directly regulating the miR172/AP2 and PANICLE PHYTOMER2 (PAP2)/Rice TFL1/CEN homolog 1 (RCN1) pathways and also by integrating other regulators, most of which are not involved in tillering regulation. These findings may also have significant implications for understanding branching regulation of other grasses and for application in rice genetic improvement.

Genetic Improvement of Root Growth and Its Relationship with Grain Yield of Wheat Cultivars in the Middle-Lower Yangtze River

2015 ◽  
Vol 41 (4) ◽  
pp. 613
Author(s):  
Zhong-Wei TIAN ◽  
Yong-Hui FAN ◽  
Mei YIN ◽  
Fang-Rui WANG ◽  
Jian CAI ◽  
...  
Keyword(s):  
Root Growth ◽  
Grain Yield ◽  
Yangtze River ◽  
Genetic Improvement ◽  
Wheat Cultivars ◽  
Lower Yangtze

Contribution of CIMMYT Wheat Germplasm to Genetic Improvement of Grain Yield in Spring Wheat of Sichuan, Yunnan, Gansu, and Xinjiang Pro-vinces

2011 ◽  
Vol 37 (10) ◽  
pp. 1752-1762 ◽  
Author(s):  
Yong ZHANG ◽  
Shi-Zhao LI ◽  
Zhen-Lu WU ◽  
Wen-Xiong YANG ◽  
Ya-Xiong YU ◽  
...  
Keyword(s):  
Grain Yield ◽  
Spring Wheat ◽  
Genetic Improvement ◽  
Wheat Germplasm

scholarly journals Gamma ray induced genetic improvement of sorghum landraces for grain yield and charcoal rot tolerance

2018 ◽  
Vol 9 (3) ◽  
pp. 894
Author(s):  
Ashok Badigannavar ◽  
G. Girish ◽  
Jayalakshmi ◽  
T.R. Ganapathi
Keyword(s):  
Grain Yield ◽  
Genetic Improvement ◽  
Gamma Ray ◽  
Charcoal Rot

Timing during translation matters: synonymous mutations in human pathologies influence protein folding and function

2018 ◽  
Vol 46 (4) ◽  
pp. 937-944 ◽  
Author(s):  
Robert Rauscher ◽  
Zoya Ignatova
Keyword(s):  
Protein Folding ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Synonymous Mutations ◽  
Translation Velocity ◽  
Disease Penetrance ◽  
And Function ◽  
Related Proteins ◽  
Translational Speed ◽  
Fine Tune

Ribosomes translate mRNAs with non-uniform speed. Translation velocity patterns are a conserved feature of mRNA and have evolved to fine-tune protein folding, expression and function. Synonymous single-nucleotide polymorphisms (sSNPs) that alter programmed translational speed affect expression and function of the encoded protein. Synergistic advances in next-generation sequencing have led to the identification of sSNPs associated with disease penetrance. Here, we draw on studies with disease-related proteins to enhance our understanding of mechanistic contributions of sSNPs to functional alterations of the encoded protein. We emphasize the importance of identification of sSNPs along with disease-causing mutations to understand genotype–phenotype relationships.

scholarly journals Roles of the GA-mediated SPL Gene Family and miR156 in the Floral Development of Chinese Chestnut (Castanea mollissima)

2019 ◽  
Vol 20 (7) ◽  
pp. 1577 ◽  
Author(s):  
Guosong Chen ◽  
Jingtong Li ◽  
Yang Liu ◽  
Qing Zhang ◽  
Yuerong Gao ◽  
...  
Keyword(s):  
Target Genes ◽  
Floral Development ◽  
Binding Protein ◽  
Expression Patterns ◽  
Evolutionary Relationship ◽  
Deciduous Tree ◽  
Main Function ◽  
Squamosa Promoter Binding Protein ◽  
Spl Genes ◽  
Castanea Mollissima

Chestnut (Castanea mollissima) is a deciduous tree species with major economic and ecological value that is widely used in the study of floral development in woody plants due its monoecious and out-of-proportion characteristics. Squamosa promoter-binding protein-like (SPL) is a plant-specific transcription factor that plays an important role in floral development. In this study, a total of 18 SPL genes were identified in the chestnut genome, of which 10 SPL genes have complementary regions of CmmiR156. An analysis of the phylogenetic tree of the squamosa promoter-binding protein (SBP) domains of the SPL genes of Arabidopsis thaliana, Populus trichocarpa, and C. mollissima divided these SPL genes into eight groups. The evolutionary relationship between poplar and chestnut in the same group was similar. A structural analysis of the protein-coding regions (CDSs) showed that the domains have the main function of SBP domains and that other domains also play an important role in determining gene function. The expression patterns of CmmiR156 and CmSPLs in different floral organs of chestnut were analyzed by real-time quantitative PCR. Some CmSPLs with similar structural patterns showed similar expression patterns, indicating that the gene structures determine the synergy of the gene functions. The application of gibberellin (GA) and its inhibitor (Paclobutrazol, PP333) to chestnut trees revealed that these exert a significant effect on the number and length of the male and female chestnut flowers. GA treatment significantly increased CmmiR156 expression and thus significantly decreased the expression of its target gene, CmSPL6/CmSPL9/CmSPL16, during floral bud development. This finding indicates that GA might indirectly affect the expression of some of the SPL target genes through miR156. In addition, RNA ligase-mediated rapid amplification of the 5′ cDNA ends (RLM-RACE) experiments revealed that CmmiR156 cleaves CmSPL9 and CmSPL16 at the 10th and 12th bases of the complementary region. These results laid an important foundation for further study of the biological function of CmSPLs in the floral development of C. mollissima.

scholarly journals The interplay between miR156/SPL13 and DFR/WD40–1 regulate drought tolerance in alfalfa

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Biruk A. Feyissa ◽  
Muhammad Arshad ◽  
Margaret Y. Gruber ◽  
Susanne E. Kohalmi ◽  
Abdelali Hannoufa
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Anthocyanin Biosynthesis ◽  
Physiological Responses ◽  
Biological Functions ◽  
Altered Expression ◽  
Transcript Levels ◽  
Squamosa Promoter Binding Protein ◽  
Fine Tune

Abstract Background Developing Medicago sativa L. (alfalfa) cultivars tolerant to drought is critical for the crop’s sustainable production. miR156 regulates various plant biological functions by silencing SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors. Results To understand the mechanism of miR156-modulated drought stress tolerance in alfalfa we used genotypes with altered expression levels of miR156, miR156-regulated SPL13, and DIHYDROFLAVONOL-4-REDUCTASE (DFR) regulating WD40–1. Previously we reported the involvement of miR156 in drought tolerance, but the mechanism and downstream genes involved in this process were not fully studied. Here we illustrate the interplay between miR156/SPL13 and WD40–1/DFR to regulate drought stress by coordinating gene expression with metabolite and physiological strategies. Low to moderate levels of miR156 overexpression suppressed SPL13 and increased WD40–1 to fine-tune DFR expression for enhanced anthocyanin biosynthesis. This, in combination with other accumulated stress mitigating metabolites and physiological responses, improved drought tolerance. We also demonstrated that SPL13 binds in vivo to the DFR promoter to regulate its expression. Conclusions Taken together, our results reveal that moderate relative miR156 transcript levels are sufficient to enhance drought resilience in alfalfa by silencing SPL13 and increasing WD40–1 expression, whereas higher miR156 overexpression results in drought susceptibility.

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