Simulation modelling of mechanical systems for intra-row weeding in a precision farming approach

Aim of study: To test new approaches to perform mechanical weeding inside the row in horticulture and tree fruit fields. The idea is to weed the row by skipping the crop by means of a rotating system instead of a traditional crosswise one. Area of study: North of Italy. Material and methods: Numerical models have been developed to simulate mechanical weeding over time by generating numerical maps to quantify the different kind of worked areas. Main results: Considering the efficiency of weed control on the row, the rotating plant-skipping system with vertical axis (RPSS-VA model) with two working tools gives the best performance index (1.1.RWA% = 95.9%). A similar performance can be obtained by the crosswise displacement plant-skipping system (CDSS model, 1.1.RWA% = 95.9 %), but with very high crosswise translation velocity (with va/vr ratio = 1/5, 1.1.RWA% = 94.5%). With regard to the outwards worked area the RPSS-VA models give the best performances (2.2.%OWAR index from 127.2% up to 282.3%). To reduce the worked area outside the row, the FBTS models give lower index (2.1.OWAR%), while the RPSS-HA works only on the row, but with the lower 1.1.RWA% index among all tested models (55.8%). Research highlights: Rotating systems resulted more efficient than traditional ones, and provide considerations on the use of electric drive power instead of hydraulic one. This study highlights also the need of new approaches in designing lighter working tools. Lastly, the proposed classification of the worked areas could be used as reference standard.

Molecular Dynamics Simulation on Behaviors of Water Nanodroplets Impinging on Moving Surfaces

Droplets impinging on solid surfaces is a common phenomenon. However, the motion of surfaces remarkably influences the dynamical behaviors of droplets, and related research is scarce. Dynamical behaviors of water nanodroplets impinging on translation and vibrating solid copper surfaces were investigated via molecular dynamics (MD) simulation. The dynamical characteristics of water nanodroplets with various Weber numbers were studied at four translation velocities, four vibration amplitudes, and five vibration periods of the surface. The results show that when water nanodroplets impinge on translation surfaces, water molecules not only move along the surfaces but also rotate around the centroid of the water nanodroplet at the relative sliding stage. Water nanodroplets spread twice in the direction perpendicular to the relative sliding under a higher surface translation velocity. Additionally, a formula for water nanodroplets velocity in the translation direction was developed. Water nanodroplets with a larger Weber number experience a heavier friction force. For cases wherein water nanodroplets impinge on vibration surfaces, the increase in amplitudes impedes the spread of water nanodroplets, while the vibration periods promote it. Moreover, the short-period vibration makes water nanodroplets bounce off the surface.

Altimeter Observations of Tropical Cyclone-generated Sea States: Spatial Analysis and Operational Hindcast Evaluation

Tropical cyclones (TC) are some of the most intense weather systems on Earth and are responsible for generating hazardous waves on the sea surface that dominate the extreme wave climate in several regions, including the Gulf of Mexico and the U.S. East Coast. Modeling these waves is crucial for engineering applications, yet it is notoriously difficult, due to TC’s compact structure and rapid evolution in space and time relative to other weather systems. To better understand the wave structure under TCs, we use satellite altimeter data paired with TC tracks. We parse the data by TC intensity and forward translation velocity, finding evidence of extended fetch. We use the altimeter data to evaluate operational hindcasts, including the US Army Corps of Engineer’s Wave Information Study, National Oceanic and Atmospheric Administration’s National Centers for Environmental Prediction Production Hindcast, and the Institut français de recherche pour l’exploitation de la mer (Ifremer) hindcast. The Ifremer hindcast (1990–2016) is examined in detail. Near the eye in the TC-centered reference frame, we find a pattern of model underestimation in the right sector and over estimation in the left and back sectors. This pattern holds, albeit modulated, across various intensities, forward translation velocities, and radii of maximum winds; the exceptions being the most intense and smallest storms, where underestimation is more severe and expands to all sectors near the TC eye.

Positive epistasis between disease-causing missense mutations and silent polymorphism with effect on mRNA translation velocity

Epistasis refers to the dependence of a mutation on other mutation(s) and the genetic context in general. In the context of human disorders, epistasis complicates the spectrum of disease symptoms and has been proposed as a major contributor to variations in disease outcome. The nonadditive relationship between mutations and the lack of complete understanding of the underlying physiological effects limit our ability to predict phenotypic outcome. Here, we report positive epistasis between intragenic mutations in the cystic fibrosis transmembrane conductance regulator (CFTR)—the gene responsible for cystic fibrosis (CF) pathology. We identified a synonymous single-nucleotide polymorphism (sSNP) that is invariant for the CFTR amino acid sequence but inverts translation speed at the affected codon. This sSNP in cis exhibits positive epistatic effects on some CF disease–causing missense mutations. Individually, both mutations alter CFTR structure and function, yet when combined, they lead to enhanced protein expression and activity. The most robust effect was observed when the sSNP was present in combination with missense mutations that, along with the primary amino acid change, also alter the speed of translation at the affected codon. Functional studies revealed that synergistic alteration in ribosomal velocity is the underlying mechanism; alteration of translation speed likely increases the time window for establishing crucial domain–domain interactions that are otherwise perturbed by each individual mutation.

Experimental study of hydrodynamic parameters regarding on geyser boiling phenomenon in glass thermosyphon using wire-mesh sensor

The thermosyphon is a type of heat exchanger that has been widely used in many applications. The use of thermosyphons has been intensified in recent years, mainly in the manufacture of solar collectors and various industrial activities. A thermosyphon is a vertical sealed tube filled with a working fluid, consisting of, from bottom to top, by an evaporator, an adiabatic section, and a condenser. The study of geyser-boiling phenomena, which occurs inside the thermosyphon is of extreme importance, therefore the experimental analysis of the parameters related to the two-phase flow (liquid-steam), such as void fraction, bubble frequency, bubble velocity, and bubble length are necessary, since these parameters have a significant influence on heat transfer. In this work, a pair of wire mesh sensors was used, a relative innovative technology to obtain experimental values of the reported quantities for measuring these parameters of slug flow in thermosyphons. An experimental setup is assembled and the sensors are coupled to the thermosyphon enabling the development of the experimental procedure. Here is presented an experimental study of a glass thermosyphon instrumented with two Wire-Mesh Sensors, in which the aforementioned slug flow hydrodynamic parameters inherent to the geyser type boiling process are measured. It was measured successfully, as a function of the heat load (110, 120, 130, 140, and 150W), the void fraction (instantly and average), liquid film thickness, translation velocity of the elongated bubbles, lengths of the bubbles, and the liquid slug (displaced by the bubble rise up). It was observed that the higher the heat load, the lower is the bubble translation velocity. For all heat loads, based on the measured length of liquid slug (consequent displacement of liquid volume), caused by bubbles rise from evaporator to condenser, it could be affirmed to some extent that both boiling regime (pool and film) exist in the evaporator. The measured average void fraction (80%) and liquid film thickness (around 2.5mm) during the elongated bubble passages were approximately constant and independent of the heat load.

Stitchless support-free 3D printing of free-form micromechanical structures with feature size on-demand

Femtosecond laser based 3D nanolithography is a powerful tool for fabricating various functional micro- and nano-objects. In this work we present several advances needed to push it from the laboratory level use to the industrial production lines. First, linear stage and galvo-scanners synchronization is employed to produce stitch-free mm-sized structures. Furthermore, it is shown that by varying objective numerical apertures (NA) from 1.4 NA to 0.45 NA, voxel size can be tuned in the range from sub μm to tens of mm, resulting in structuring rates between 1809 μm3/s and 313312 μm3/s at 1 cm/s translation velocity achieved via simultaneous movement of linear stages and scanners. Discovered voxel/throughput scaling peculiarities show good agreement to ones acquired with numerical modeling. Furthermore, support-free 3D printing of complex structures is demonstrated. It is achieved by choosing pre-polymer that is in hard gel form during laser writing and acts as a dissolvable support during manufacturing. All of this is combined to fabricate micromechanical structures. First, 1:40 aspect ratio cantilever and 1.5 mm diameter single-helix spring capable of sustaining extreme deformations for prolonged movement times (up to 10000 deformation cycles) are shown. Then, free-movable highly articulated intertwined micromechanical spider and squids (overall size up to 10 mm) are printed and their movement is tested. The presented results are discussed in the broader sense, touching on the stitching/throughput dilemma and comparing it to the standard microstereolithography. It is shown where multiphoton polymerization can outpace standard stereolithography in terms of throughput while still maintaining superior resolution and higher degree of freedom in terms of printable geometries.

Microwheels on microroads: Enhanced translation on topographic surfaces

Microbot locomotion is challenging because of the reversible nature of microscale fluid flow, a limitation that can be overcome by breaking flowfield symmetry with a nearby surface. We have used this strategy with rotating wheel-shaped microbots, microwheels (μwheels), that roll on surfaces leading to enhanced propulsion and fast translation speeds. Despite this, studies to date on flat surfaces show that μwheels roll inefficiently with substantial slip. Taking inspiration from the mathematics of roads and wheels, we demonstrate that μwheel velocities can be significantly enhanced by changing microroad topography. Here, we observe that periodic bumps in the road can be used to enhance the traction between μwheels and nearby walls. Whereas continuous μwheel rotation with slip is observed on flat surfaces, a combination of rotation with slip and nonslip flip occurs when μwheels roll on surfaces with periodic features, resulting in up to fourfold enhancement in translation velocity. The unexpectedly fast rolling speed of μwheels on bumpy roads can be attributed to the hydrodynamic coupling between μwheels and road surface features, allowing nonslip rotation of entire wheels along one of their stationary edges. This road-wheel coupling can also be used to enhance μwheel sorting and separation where the gravitational potential energy barrier induced by topographic surfaces can lead to motion in only one direction and to different rolling speeds between isomeric wheels, allowing one to separate them not based on size but on symmetry.

Timing during translation matters: synonymous mutations in human pathologies influence protein folding and function

Ribosomes translate mRNAs with non-uniform speed. Translation velocity patterns are a conserved feature of mRNA and have evolved to fine-tune protein folding, expression and function. Synonymous single-nucleotide polymorphisms (sSNPs) that alter programmed translational speed affect expression and function of the encoded protein. Synergistic advances in next-generation sequencing have led to the identification of sSNPs associated with disease penetrance. Here, we draw on studies with disease-related proteins to enhance our understanding of mechanistic contributions of sSNPs to functional alterations of the encoded protein. We emphasize the importance of identification of sSNPs along with disease-causing mutations to understand genotype–phenotype relationships.

SORET AND DUFOUR EFFECTS ON UNSTEADY MHD FREE CONVECTIVE FLOW OF MICROPOLAR FLUID WITH OSCILLATORY PLATE VELOCITY CONSIDERING VISCOUS DISSIPATION EFFECTS

The present paper deals with the study of micropolar fluid flow of an unsteady incompressible free convection heat and mass transfer flow past a semi-infinite vertical plate fixed firmly in porous medium with the influence of heat absorption, viscous dissipation, Soret, Dufour and chemical reaction has been analyzed. The governing partial differential equations are transformed into a set of coupled partial differential equations using suitable dimensionless quantities. The resulting non-dimensional boundary value problem is solved by the Galerkin finite element method. The effects of different pertinent parameters on translation velocity, microrotation, temperature and concentration distributions along boundary layer have been represented with the help of graphs. Under special case, comparison of the Skin friction, Wall couple stress, Nusselt number and Sherwood number are made with the Numerical results available from the literature obtained through analytical approach and found to be in good agreement.