scholarly journals Immunogenicity and efficacy of the COVID-19 candidate vector vaccine MVA-SARS-2-S in preclinical vaccination

2021 ◽  
Vol 118 (28) ◽  
pp. e2026207118
Author(s):  
Alina Tscherne ◽  
Jan Hendrik Schwarz ◽  
Cornelius Rohde ◽  
Alexandra Kupke ◽  
Georgia Kalodimou ◽  
...  
Keyword(s):  
Infectious Agent ◽  
Growth Characteristics ◽  
Scale Production ◽  
Suitable Candidate ◽  
S Protein ◽  
Vector Vaccine ◽  
Modified Vaccinia Virus Ankara ◽  
Efficacious Vaccine ◽  
Industrial Scale Production

Severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) has emerged as the infectious agent causing the pandemic coronavirus disease 2019 (COVID-19) with dramatic consequences for global human health and economics. Previously, we reached clinical evaluation with our vector vaccine based on modified vaccinia virus Ankara (MVA) against the Middle East respiratory syndrome coronavirus (MERS-CoV), which causes an infection in humans similar to SARS and COVID-19. Here, we describe the construction and preclinical characterization of a recombinant MVA expressing full-length SARS-CoV-2 spike (S) protein (MVA-SARS-2-S). Genetic stability and growth characteristics of MVA-SARS-2-S, plus its robust expression of S protein as antigen, make it a suitable candidate vaccine for industrial-scale production. Vaccinated mice produced S-specific CD8+ T cells and serum antibodies binding to S protein that neutralized SARS-CoV-2. Prime-boost vaccination with MVA-SARS-2-S protected mice sensitized with a human ACE2-expressing adenovirus from SARS-CoV-2 infection. MVA-SARS-2-S is currently being investigated in a phase I clinical trial as aspirant for developing a safe and efficacious vaccine against COVID-19.

scholarly journals Immunogenicity and efficacy of the COVID-19 candidate vector vaccine MVA SARS 2 S in preclinical vaccination

2021 ◽  
Author(s):  
Alina Tscherne ◽  
Jan Hendrik Schwarz ◽  
Cornelius Rohde ◽  
Alexandra Kupke ◽  
Georgia Kalodimou ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Clinical Evaluation ◽  
Virus Disease ◽  
Protective Efficacy ◽  
Infectious Agent ◽  
Full Length ◽  
Candidate Vaccine ◽  
Scale Production ◽  
S Protein ◽  
Vector Vaccine

AbstractThe severe acute respiratory syndrome (SARS) coronavirus 2 (SARS-CoV-2) has emerged as the infectious agent causing the pandemic coronavirus disease 2019 (COVID-19) with dramatic consequences for global human health and economics. Previously, we reached clinical evaluation with our vector vaccine based on vaccinia virus MVA against the Middle East respiratory syndrome coronavirus (MERS-CoV), which causes an infection in humans similar to SARS and COVID-19. Here, we describe the construction and preclinical characterization of a recombinant MVA expressing full-length SARS-CoV-2 spike (S) protein (MVA-SARS-2-S). Genetic stability and growth characteristics of MVA-SARS-2-S, plus its robust synthesis of S antigen, make it a suitable candidate vaccine for industrial scale production. Vaccinated mice produced S antigen-specific CD8+ T cells and serum antibodies binding to S glycoprotein that neutralized SARS-CoV-2. Prime-boost vaccination with MVA-SARS-2-S protected mice sensitized with a human ACE2-expressing adenovirus from SARS-CoV-2 infection. MVA-SARS-2-S is currently being investigated in a phase I clinical trial as aspirant for developing a safe and efficacious vaccine against COVID-19.Significance StatementThe highly attenuated vaccinia virus MVA is licensed as smallpox vaccine, and as vector it is a component of the approved Adenovirus-MVA-based prime-boost vaccine against Ebola virus disease. Here we provide results from testing the COVID-19 candidate vaccine MVA-SARS-2-S, a poxvirus-based vector vaccine that proceeded to clinical evaluation. When administered by intramuscular inoculation, MVA-SARS-2-S expresses and safely delivers the full-length SARS-CoV-2 spike (S) protein, inducing balanced SARS-CoV-2-specific cellular and humoral immunity, and protective efficacy in vaccinated mice. Substantial clinical experience has already been gained with MVA vectors using homologous and heterologous prime-boost applications, including the immunization of children and immunocompromised individuals. Thus, MVA-SARS-2-S represents an important resource for developing further optimized COVID-19 vaccines.

Synthesis and characterization of poly(ω-pentadecalactone) for its industrial-scale production

2015 ◽  
Vol 31 (4) ◽  
pp. 640-644 ◽  
Author(s):  
Qing Chang ◽  
Li Li ◽  
Dalei Yang ◽  
Mingyao Zhang ◽  
Minh-Tan Ton-That ◽  
...  
Keyword(s):  
Industrial Scale ◽  
Synthesis And Characterization ◽  
Scale Production ◽  
Industrial Scale Production

scholarly journals Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis

ACS Omega ◽  
2020 ◽  
Author(s):  
Natalia G. Herrera ◽  
Nicholas C. Morano ◽  
Alev Celikgil ◽  
George I. Georgiev ◽  
Ryan J. Malonis ◽  
...  
Keyword(s):  
Antigenic Analysis ◽  
S Protein

Formation Of Single-Wall Carbon Nanotubes Forrest Assemblies On Metal Surfaces

2002 ◽  
Vol 734 ◽  
Author(s):  
Debjit Chattopadhyay ◽  
Izabela Galeska ◽  
Fotios Papadimitrakopoulos
Keyword(s):  
Carbon Nanotubes ◽  
Surface Coverage ◽  
Iron Hydroxide ◽  
Growth Characteristics ◽  
Self Organization ◽  
Single Wall Carbon Nanotubes ◽  
Unique Challenge ◽  
Single Wall Carbon ◽  
Topological Characteristics

ABSTRACTLearning how to purify and manipulate single wall carbon nanotubes (SWNTs) presents a unique challenge in material science. The processing-related difficulties of these long nano-fibers stem from their high aspect ratio, rigidity and the profound hydrophobic attractions along their tubular walls. Shortening them into discrete segments, with lengths from tens to hundreds of nanometers, presents a viable methodology to alleviate the shape-induced intractability. In addition, the metal-assisted self-organization of these nanosized objects into nano-forest geometries with dense perpendicular surface grafting, demonstrates that such nanosized objects hold significant promise for the development of nanoscale devices. This paper will present an extensive characterization of the topological characteristics of these assemblies, along with their surface coverage, growth characteristics and height fluctuation on iron hydroxide substrates.

Semi-industrial Scale Production of a New Yeast with Probiotic Traits, Cryptococcus sp. YMHS, Isolated from the Red Sea

2017 ◽  
Vol 10 (1) ◽  
pp. 77-88 ◽  
Author(s):  
Ashraf F. El-Baz ◽  
Hesham A. El-Enshasy ◽  
Yousseria M. Shetaia ◽  
Hoda Mahrous ◽  
Nor Zalina Othman ◽  
...  
Keyword(s):  
Red Sea ◽  
Industrial Scale ◽  
Scale Production ◽  
New Yeast ◽  
Industrial Scale Production

DNA-Directed Patterning for Versatile Validation and Characterization of a Lipid-Based Nanoparticle Model of SARS-CoV-2

2021 ◽  
Author(s):  
Molly Kozminsky ◽  
Thomas Carey ◽  
Lydia L. Sohn
Keyword(s):  
High Throughput ◽  
Glass Substrate ◽  
Rapid Development ◽  
Neutralizing Antibody ◽  
S Protein ◽  
Biological Relevance ◽  
The Face ◽  
Great Control ◽  
Tetraspanin Cd63

Lipid-based nanoparticles have risen to the forefront of the COVID-19 pandemic—from encapsulation of vaccine components to modeling the virus, itself. Their rapid development in the face of the volatile nature of the pandemic requires high-throughput, highly flexible methods for characterization. DNA-directed patterning is a versatile method to immobilize and segregate lipid-based nanoparticles for subsequent analysis. DNA-directed patterning selectively conjugates oligonucleotides onto a glass substrate and then hybridizes them to complementary oligonucleotides tagged to the liposomes, thereby patterning them with great control and precision. The power of this method is demonstrated by characterizing a novel recapitulative lipid-based nanoparticle model of SARS-CoV-2 —S-liposomes— which present the SARS-CoV-2 spike (S) protein on their surfaces. Patterning of a mixture of S-liposomes and liposomes that display the tetraspanin CD63 into discrete regions of a substrate is used to show that ACE2 specifically binds to S-liposomes. Importantly, DNA-directed patterning of S-liposomes is used to verify the performance of a commercially available neutralizing antibody against the S protein. Ultimately, the introduction of S-liposomes to ACE2-expressing cells demonstrates the biological relevance of DNA-directed patterning. Overall, DNA-directed patterning enables a wide variety of custom assays for the characterization of any lipid-based nanoparticle.

Isolasi dan Potensi Enzim Hidrolase Bakteri Simbion Padina sp. dari Pantai Lengkuas Belitung

2021 ◽  
Vol 23 (1) ◽  
pp. 11-17
Author(s):  
Siti Nur Jannah ◽  
Yumna Rahmadias Hanifa ◽  
Adi Budi Utomo ◽  
Ashar Kurnia Dian Prambodo ◽  
Arina Tri Lunggani
Keyword(s):  
Marine Organism ◽  
Marine Ecosystem ◽  
Gram Negative Bacteria ◽  
Scale Production ◽  
Market Demand ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Plate Method ◽  
Sea Use ◽  
Industrial Scale Production

Marine organism is one of the riches in the ocean of Indonesia. The benefits of sea use for new products produced are widely used and have high market demand. Enzymes that have marine interests have unique properties and have good benefits for industry. This study aims to isolate the bacteria that have symbionts with Padina sp and determine the potential of the enzyme hydrolase produced by these bacteria. Isolation is done by the spread plate method. Pure isolates obtained were then tested for the potential of the enzyme hydrolase on selective media. Clear zone measurements are performed to determine which bacterial isolates are good for enzyme production. The results obtained by 6 isolates of pure bacteria, all of which include Gram negative bacteria that form bacilli. All isolates had the ability to produce different Protease, Lipase, Amylase and Cellulase enzymes. The enzymes obtained from these symbiotic bacteria are expected to be used for industrial-scale production in Indonesia. In addition, the presence of this symbiont bacteria is able to reduce the level of exploitation of Padina sp and contribute to preserving the marine ecosystem.

scholarly journals Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1197 ◽  
Author(s):  
Warren Blunt ◽  
David Levin ◽  
Nazim Cicek
Keyword(s):  
Production Costs ◽  
Scale Production ◽  
Fed Batch ◽  
Continuous Bioreactor ◽  
Carbon Substrates ◽  
Pure Cultures ◽  
High Production ◽  
Operating Strategies ◽  
Major Factors ◽  
Industrial Scale Production

Microbial polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that may alleviate some of the environmental burden of petroleum-derived polymers. The requirements for carbon substrates and energy for bioreactor operations are major factors contributing to the high production costs and environmental impact of PHAs. Improving the process productivity is an important aspect of cost reduction, which has been attempted using a variety of fed-batch, continuous, and semi-continuous bioreactor systems, with variable results. The purpose of this review is to summarize the bioreactor operations targeting high PHA productivity using pure cultures. The highest volumetric PHA productivity was reported more than 20 years ago for poly(3-hydroxybutryate) (PHB) production from sucrose (5.1 g L−1 h−1). In the time since, similar results have not been achieved on a scale of more than 100 L. More recently, a number fed-batch and semi-continuous (cyclic) bioreactor operation strategies have reported reasonably high productivities (1 g L−1 h−1 to 2 g L−1 h−1) under more realistic conditions for pilot or industrial-scale production, including the utilization of lower-cost waste carbon substrates and atmospheric air as the aeration medium, as well as cultivation under non-sterile conditions. Little development has occurred in the area of fully continuously fed bioreactor systems over the last eight years.

Industrial Scale Production of L-B Layers

1989 ◽  
pp. 41-49 ◽  
Author(s):  
O. Albrecht ◽  
T. Ginnai ◽  
A. Harrington ◽  
D. Marr-Leisy ◽  
V. Rodov
Keyword(s):  
Industrial Scale ◽  
Scale Production ◽  
Industrial Scale Production
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