scholarly journals Mouse model of human beta zero thalassemia: targeted deletion of the mouse beta maj- and beta min-globin genes in embryonic stem cells.

1995 ◽  
Vol 92 (20) ◽  
pp. 9259-9263 ◽  
Author(s):  
D. J. Ciavatta ◽  
T. M. Ryan ◽  
S. C. Farmer ◽  
T. M. Townes
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mouse Model ◽  
Embryonic Stem ◽  
Globin Genes ◽  
Targeted Deletion

scholarly journals Inactivation of mouse alpha-globin gene by homologous recombination: mouse model of hemoglobin H disease

Blood ◽  
1996 ◽  
Vol 88 (5) ◽  
pp. 1846-1851 ◽  
Author(s):  
J Chang ◽  
RH Lu ◽  
SM Xu ◽  
J Meneses ◽  
K Chan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mouse Model ◽  
Knockout Mice ◽  
Globin Gene ◽  
Embryonic Stem ◽  
Globin Genes ◽  
Human Homologue ◽  
Alpha Thalassemia ◽  
Alpha Globin ◽  
Hemoglobin H Disease

We have disrupted the 5′ locus of the duplicated adult alpha-globin genes by gene targeting in the mouse embryonic stem cells and created mice with alpha-thalassemia syndromes. The heterozygous knockout mice (.alpha/alpha alpha) are asymptomatic like the silent carriers in humans whereas the homozygous knockout mice (.alpha/.alpha) show hemolytic anemia. Mice with three dysfunctional alpha-globin genes generated by breeding the 5′ alpha-globin knockouts (.alpha/alpha alpha) and the deletion type alpha-thalassemia mice (../alpha alpha) produce severe hemoglobin H disease and they die in utero. These results indicate that the 5′ alpha-globin gene is the predominant locus in mice, and suggest that it is even more dominant than its human homologue.

Use of Differentiating Embryonic Stem Cells in the Parkinsonian Mouse Model

2006 ◽  
pp. 485-494
Author(s):  
Fumihiko Nishimura ◽  
Hayato Toriumi ◽  
Shigeaki Ishizaka ◽  
Toshisuke Sakaki ◽  
Masahide Yoshikawa
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mouse Model ◽  
Embryonic Stem

Long targeting arms do not increase the efficiency of homologous recombination in the β-globin locus of murine embryonic stem cells

Blood ◽  
2003 ◽  
Vol 102 (4) ◽  
pp. 1531-1533 ◽  
Author(s):  
Zhi Hong Lu ◽  
Jason T. Books ◽  
Richard M. Kaufman ◽  
Timothy J. Ley
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Homologous Recombination ◽  
Globin Gene ◽  
Embryonic Stem ◽  
Regulatory Sequences ◽  
Globin Genes ◽  
Gene Correction ◽  
Murine Embryonic Stem Cells ◽  
Alternative Approaches

Abstract The correction of mutant β-globin genes has long been a therapeutic goal for patients with β-thalassemia or hemoglobinopathies. The use of homologous recombination (HR) to achieve this goal is an attractive approach because it eliminates the need to include regulatory sequences in the therapeutic construct, and it eliminates mutagenesis induced by random integration. However, HR is a very inefficient process for gene correction, and its efficiency is probably locus dependent. The length of targeting arms is thought to be a determinant of targeting efficiency, so we compared the ability of standard (8-kb) versus very long (16-, 24-, and 110-kb) regions of homology to correct a mutant murine β-globin gene in embryonic stem cells. Increasing the length of the targeting sequences did not increase the efficiency of HR in this locus, suggesting that alternative approaches will be required to improve the efficiency of this approach for globin gene correction.

scholarly journals Human Embryonic Stem Cells Differentiated to Lung Lineage-Specific Cells Ameliorate Pulmonary Fibrosis in a Xenograft Transplant Mouse Model

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33165 ◽  
Author(s):  
Ena Ray Banerjee ◽  
Michael A. Laflamme ◽  
Thalia Papayannopoulou ◽  
Michael Kahn ◽  
Charles E. Murry ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Pulmonary Fibrosis ◽  
Mouse Model ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem

scholarly journals Replication Initiation Patterns in the β-Globin Loci of Totipotent and Differentiated Murine Cells: Evidence for Multiple Initiation Regions

2002 ◽  
Vol 22 (2) ◽  
pp. 442-452 ◽  
Author(s):  
Mirit I. Aladjem ◽  
Luo Wei Rodewald ◽  
Chii Mai Lin ◽  
Sarah Bowman ◽  
Daniel M. Cimbora ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dna Replication ◽  
Embryonic Stem Cells ◽  
Chromatin Structure ◽  
Embryonic Stem ◽  
Replication Initiation ◽  
Globin Genes ◽  
Primary Sequence ◽  
Differentiated Cells ◽  
Hypersensitive Sites

ABSTRACT The replication initiation pattern of the murine β-globin locus was analyzed in totipotent embryonic stem cells and in differentiated cell lines. Initiation events in the murine β-globin locus were detected in a region extending from the embryonic Ey gene to the adult βminor gene, unlike the restricted initiation observed in the human locus. Totipotent and differentiated cells exhibited similar initiation patterns. Deletion of the region between the adult globin genes did not prevent initiation in the remainder of the locus, suggesting that the potential to initiate DNA replication was not contained exclusively within the primary sequence of the deleted region. In addition, a deletion encompassing the six identified 5′ hypersensitive sites in the mouse locus control region had no effect on initiation from within the locus. As this deletion also did not affect the chromatin structure of the locus, we propose that the sequences determining both chromatin structure and replication initiation lie outside the hypersensitive sites removed by the deletion.

scholarly journals Transplantation of Reprogrammed Embryonic Stem Cells Improves Visual Function in a Mouse Model for Retinitis Pigmentosa

2010 ◽  
Vol 89 (8) ◽  
pp. 911-919 ◽  
Author(s):  
Nan-Kai Wang ◽  
Joaquin Tosi ◽  
Jennifer Mie Kasanuki ◽  
Chai Lin Chou ◽  
Jian Kong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Mouse Model ◽  
Retinitis Pigmentosa ◽  
Visual Function ◽  
Embryonic Stem
Sign in / Sign up

Export Citation Format

Share Document