scholarly journals Role of Sp1 and Sp3 in the Nutrient-regulated Expression of the Human Asparagine Synthetase Gene

2002 ◽  
Vol 277 (19) ◽  
pp. 16585-16591 ◽  
Author(s):  
Van Leung-Pineda ◽  
Michael S. Kilberg
Keyword(s):  
Asparagine Synthetase ◽  
Regulated Expression

Reorganization of membrane contacts prior to apoptosis in the Drosophila retina: the role of the IrreC-rst protein

Development ◽  
1996 ◽  
Vol 122 (6) ◽  
pp. 1931-1940 ◽  
Author(s):  
C. Reiter ◽  
T. Schimansky ◽  
Z. Nie ◽  
K.F. Fischbach
Keyword(s):  
Pattern Formation ◽  
Pigment Cells ◽  
Immunoglobulin Superfamily ◽  
Specific Cell ◽  
Axonal Pathfinding ◽  
Single Row ◽  
Cell Contacts ◽  
Regulated Expression ◽  
Membrane Contacts

The final step of pattern formation in the developing retina of Drosophila is the elimination of excess cells between ommatidia and the differentiation of the remaining cells into secondary and tertiary pigment cells. Temporally and spatially highly regulated expression of the irregular chiasmC-roughest protein, an adhesion molecule of the immunoglobulin superfamily known to be involved in axonal pathfinding, is essential for correct sorting of cell-cell contacts in the pupal retina without which the ensuing wave of apoptosis does not occur. Irregular chiasmC-roughest accumulates strongly at the borders between primary pigment and interommatidial cells. Mutant and misexpression analysis show that this accumulation of the irregular chiasmC-roughest protein is necessary for aligning interommatidial cells in a single row. This reorganisation is a prerequisite for the identification of death candidates. Irregular chiasmC-roughest function in retinal development demonstrates the importance of specific cell contacts for assignment of the apoptotic fate.

Regulated expression of a murine class I gene in transgenic mice

1986 ◽  
Vol 6 (4) ◽  
pp. 1339-1342
Author(s):  
C Bieberich ◽  
G Scangos ◽  
K Tanaka ◽  
G Jay
Keyword(s):  
Transgenic Mice ◽  
Germ Line ◽  
Inbred Mice ◽  
Class I ◽  
Endogenous Gene ◽  
Histocompatibility Complex ◽  
Unique System ◽  
Regulated Expression ◽  
I Gene

The major histocompatibility complex class I genes play an essential role in the immune presentation of aberrant cells. To gain further insight into the regulation of the expression of these class I genes and to better define the functions of their protein products, we made use of the technique of gene transfer into the germ line of inbred mice. With the use of locus-specific DNA probes, we observed that a transgenic class I gene was expressed in a tissue-dependent fashion analogous to that of an endogenous class I gene. In addition, the level of expression of the transgenic gene was substantially higher that that of the endogenous gene. The availability of transgenic mice properly expressing a foreign murine class I gene provides a unique system to further define the role of the class I antigens in the maturation of the immune response and in determining the malignant and metastatic phenotypes of tumor cells.

scholarly journals CsWRKY25 Improves Resistance of Citrus Fruit to Penicillium digitatum via Modulating Reactive Oxygen Species Production

2022 ◽  
Vol 12 ◽  
Author(s):  
Wenjun Wang ◽  
Ting Li ◽  
Qi Chen ◽  
Shixiang Yao ◽  
Lili Deng ◽  
...  
Keyword(s):  
Target Genes ◽  
Citrus Fruit ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Transcription Level ◽  
Pr Genes ◽  
Citrus Peel ◽  
Regulated Expression ◽  
Transient Overexpression ◽  
Species Production

WRKY transcription factors (TFs) play crucial roles in the regulation of biotic stress. Citrus is the most productive fruit in the world. It is of great value to investigate the regulatory molecular mechanism of WRKYs in improving disease resistance. In this research, the transcription level of CsWRKY25 was upregulated in P. digitatum infected citrus peel, and CsWRKY25 activated the expression of three target genes (RbohB, RbohD, and PR10). Besides, the Agrobacterium-mediated transient overexpression of CsWRKY25 has also been shown to enhance resistance to P. digitatum in citrus, and caused the accumulation of hydrogen peroxide and lignin. The accumulation of ROS also activated the antioxidant system, the catalase (CAT), peroxidase (POD), and cinnamyl alcohol dehydrogenase (CAD) genes were significant upregulated, leading to activation of antioxidant enzymes. In addition, the up-regulated expression of MPK5 and MPK6 genes suggested that the regulatory role of CsWRKY25 might be related to the phosphorylation process. In conclusion, CsWRKY25 could enhance the resistance to P. digitatum via modulating ROS production and PR genes in citrus peel.

The Role of Tumor-related LncRNA PART1 in cancer

2021 ◽  
Vol 27 ◽  
Author(s):  
Jinlan Chen ◽  
Enqing Meng ◽  
Yexiang Lin ◽  
Yujie Shen ◽  
Chengyu Hu ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Malignant Tumors ◽  
Migration And Invasion ◽  
Signal Pathways ◽  
Toll Like Receptor ◽  
Non Coding Rna ◽  
Regulated Expression ◽  
The One ◽  
Long Non Coding Rna

Background: As we all know, long non-coding RNA (lncRNA) affects tumor progression, which has caused a great upsurge in recent years. It can also affect the growth, migration, and invasion of tumors. When we refer to the abnormal expression of lncRNA, we will find it associated with malignant tumors. In addition, lncRNA has been proved to be a key targeted gene for the treatment of some diseases. PART1, a member of lncRNA, has been reported as a regulator in the process of tumor occurrence and development. This study aims to reveal the biological functions, specific mechanisms, and clinical significance of PART1 in various tumor cells. Methods: Through the careful search of PUBMED, the mechanisms of the effect of PART1 on tumorigenesis and development are summarized. Results: On the one hand, the up-regulated expression of PART1 plays a tumor-promoting role in tumors, including lung cancer, prostate cancer, bladder cancer and so on. On the other hand, PART1 is down-regulated in gastric cancer, glioma and other tumors to play a tumor inhibitory role. In addition, PART1 regulates tumor growth mainly by targeting microRNA such as miR-635, directly regulating the expression of proteins such as FUS/EZH2, affecting signal pathways such as the Toll-like receptor pathway, or regulating immune cells. Conclusion: PART1 is closely related to tumors by regulating a variety of molecular mechanisms. In addition, PART1 can be used as a clinical marker for the early diagnosis of tumors and plays an important role in tumor-targeted therapy.

scholarly journals Integrative omics analysis in Pandanus odorifer (Forssk.) Kuntze reveals the role of Asparagine synthetase in salinity tolerance

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Deo Rashmi ◽  
Vitthal T. Barvkar ◽  
Altafhusain Nadaf ◽  
Swapnil Mundhe ◽  
Narendra Y. Kadoo
Keyword(s):  
Salinity Tolerance ◽  
Asparagine Synthetase ◽  
Omics Analysis ◽  
Integrative Omics

scholarly journals Canonical and noncanonical TGF-β signaling regulate fibrous tissue differentiation in the axial skeleton

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sade W. Clayton ◽  
Ga I. Ban ◽  
Cunren Liu ◽  
Rosa Serra
Keyword(s):  
Protein Synthesis ◽  
Fibrous Tissue ◽  
Axial Skeleton ◽  
Tissue Differentiation ◽  
Connective Tissues ◽  
Signaling Mechanism ◽  
Regulated Expression ◽  
New Protein

AbstractPreviously, we showed that embryonic deletion of TGF-β type 2 receptor in mouse sclerotome resulted in defects in fibrous connective tissues in the spine. Here we investigated how TGF-β regulates expression of fibrous markers: Scleraxis, Fibromodulin and Adamtsl2. We showed that TGF-β stimulated expression of Scleraxis mRNA by 2 h and Fibromodulin and Adamtsl2 mRNAs by 8 h of treatment. Regulation of Scleraxis by TGF-β did not require new protein synthesis; however, protein synthesis was required for expression of Fibromodulin and Adamtsl2 indicating the necessity of an intermediate. We subsequently showed Scleraxis was a potential intermediate for TGF-β-regulated expression of Fibromodulin and Adamtsl2. The canonical effector Smad3 was not necessary for TGF-β-mediated regulation of Scleraxis. Smad3 was necessary for regulation of Fibromodulin and Adamtsl2, but not sufficient to super-induce expression with TGF-β treatment. Next, the role of several noncanonical TGF-β pathways were tested. We found that ERK1/2 was activated by TGF-β and required to regulate expression of Scleraxis, Fibromodulin, and Adamtsl2. Based on these results, we propose a model in which TGF-β regulates Scleraxis via ERK1/2 and then Scleraxis and Smad3 cooperate to regulate Fibromodulin and Adamtsl2. These results define a novel signaling mechanism for TGFβ-mediated fibrous differentiation in sclerotome.

High levels of asparagine synthetase in hypocotyls of pine seedlings suggest a role of the enzyme in re-allocation of seed-stored nitrogen

Planta ◽  
2006 ◽  
Vol 224 (1) ◽  
pp. 83-95 ◽  
Author(s):  
Rafael A. Cañas ◽  
Fernando de la Torre ◽  
Francisco M. Cánovas ◽  
Francisco R. Cantón
Keyword(s):  
Asparagine Synthetase ◽  
Pine Seedlings

scholarly journals Impairment of PGC-1 Alpha Up-Regulation Enhances Nitrosative Stress in the Liver during Acute Pancreatitis in Obese Mice

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 887
Author(s):  
Sergio Rius-Pérez ◽  
Isabel Torres-Cuevas ◽  
María Monsalve ◽  
Francisco J. Miranda ◽  
Salvador Pérez
Keyword(s):  
Acute Pancreatitis ◽  
Nitrosative Stress ◽  
Energy Charge ◽  
Pancreatic Tissue ◽  
Obese Mice ◽  
Antioxidant Genes ◽  
Regulated Expression ◽  
Distant Organ ◽  
Nos2 Expression

Acute pancreatitis is an inflammatory process of the pancreatic tissue that often leads to distant organ dysfunction. Although liver injury is uncommon in acute pancreatitis, obesity is a risk factor for the development of hepatic complications. The aim of this work was to evaluate the role of PGC-1α in inflammatory response regulation in the liver and its contribution to the detrimental effect of obesity on the liver during acute pancreatitis. For this purpose, we induced acute pancreatitis by cerulein in not only wild-type (WT) and PGC-1α knockout (KO) mice, but also in lean and obese mice. PGC-1α levels were up-regulated in the mice livers with pancreatitis. The increased PGC-1α levels were bound to p65 to restrain its transcriptional activity toward Nos2. Lack of PGC-1α favored the assembly of the p65/phospho-STAT3 complex, which promoted Nos2 expression during acute pancreatitis. The increased transcript Nos2 levels and the pro-oxidant liver status caused by the down-regulated expression of the PGC-1α-dependent antioxidant genes enhanced nitrosative stress and decreased energy charge in the livers of the PGC-1α KO mice with pancreatitis. It is noteworthy that the PGC-1α levels lowered in the obese mice livers, which increased the Nos2 mRNA expression and protein nitration levels and decreased energy charge during pancreatitis. In conclusion, obesity impairs PGC-1α up-regulation in the liver to cause nitrosative stress during acute pancreatitis.

scholarly journals Role of Cell Cycle-regulated Expression in the Localized Incorporation of Cell Wall Proteins in Yeast

2006 ◽  
Vol 17 (7) ◽  
pp. 3267-3280 ◽  
Author(s):  
Gertien J. Smits ◽  
Laura R. Schenkman ◽  
Stanley Brul ◽  
John R. Pringle ◽  
Frans M. Klis
Keyword(s):  
Cell Cycle ◽  
Cell Wall ◽  
Mechanical Damage ◽  
Cell Wall Proteins ◽  
Functional Interaction ◽  
Promoter Regions ◽  
Septum Formation ◽  
Regulated Expression ◽  
Mother Cells

The yeast cell wall is an essential organelle that protects the cell from mechanical damage and antimicrobial peptides, participates in cell recognition and adhesion, and is important for the generation and maintenance of normal cell shape. We studied the localization of three covalently bound cell wall proteins in Saccharomyces cerevisiae. Tip1p was found only in mother cells, whereas Cwp2p was incorporated in small-to-medium–sized buds. When the promoter regions of TIP1 and CWP2 (responsible for transcription in early G1and S/G2phases, respectively) were exchanged, the localization patterns of Tip1p and Cwp2p were reversed, indicating that the localization of cell wall proteins can be completely determined by the timing of transcription during the cell cycle. The third protein, Cwp1p, was incorporated into the birth scar, where it remained for several generations. However, we could not detect any role of Cwp1p in strengthening the birth scar wall or any functional interaction with the proteins that mark the birth scar pole as a potential future budding site. Promoter-exchange experiments showed that expression in S/G2phase is necessary but not sufficient for the normal localization of Cwp1p. Studies of mutants in which septum formation is perturbed indicate that the normal asymmetric localization of Cwp1p also depends on the normal timing of septum formation, composition of the septum, or both.

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