Cross-talk between chromatin acetylation and SUMOylation of tripartite motif–containing protein 24 (TRIM24) impacts cell adhesion

The membrane-trafficking pathway mediated by tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) in neurons is still unknown. We show herein that TI-VAMP expression is necessary for neurite outgrowth in PC12 cells and hippocampal neurons in culture. TI-VAMP interacts with plasma membrane and endosomal target soluble N-ethylmaleimide-sensitive factor attachment protein receptors, suggesting that TI-VAMP mediates a recycling pathway. L1, a cell-cell adhesion molecule involved in axonal outgrowth, colocalized with TI-VAMP in the developing brain, neurons in culture, and PC12 cells. Plasma membrane L1 was internalized into the TI-VAMP–containing compartment. Silencing of TI-VAMP resulted in reduced expression of L1 at the plasma membrane. Finally, using the extracellular domain of L1 and N-cadherin immobilized on beads, we found that the silencing of TI-VAMP led to impaired L1- but not N-cadherin–mediated adhesion. Furthermore, TI-VAMP- but not synaptobrevin 2-containing vesicles accumulated at the site of the L1 bead-cell junction. We conclude that TI-VAMP mediates the intracellular transport of L1 and that L1-mediated adhesion controls this membrane trafficking, thereby suggesting an important cross talk between membrane trafficking and cell-cell adhesion.

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Novel equine conceptus–endometrial interactions on Day 16 of pregnancy based on RNA sequencing

Reproduction Fertility and Development ◽

10.1071/rd14489 ◽

2016 ◽

Vol 28 (11) ◽

pp. 1712 ◽

Cited By ~ 17

Author(s):

C. Klein

Keyword(s):

Cell Adhesion ◽

Growth Factors ◽

Next Generation Sequencing ◽

Rna Sequencing ◽

Cross Talk ◽

Transcriptome Analysis ◽

Interaction Analysis ◽

Cell Communication ◽

Next Generation ◽

Generation Sequencing

Maintenance of pregnancy is dependent on the exchange of signals between the conceptus and the endometrium. The objective of this study was to use next-generation sequencing to determine transcriptome blueprints of the conceptus and endometrium 16 days after ovulation in the horse. There were 7760 and 10 182 genes expressed in the conceptus and endometrium, respectively, of which 7029 were present in both. Genes related to developmental processes were enriched among conceptus-specific transcripts, whereas many endometrium-specific genes had known roles in cell communication, cell adhesion and response to stimuli. The integrin signalling pathway was overrepresented in both transcriptomes. In that regard, it was hypothesised that integrins ITGA5B1 and ITGAVB3 interact with conceptus-derived fibrinogen, potentially contributing to cessation of conceptus mobility. That several growth factors and their corresponding receptors (e.g. HDGF, NOV, CYR61, CTGF, HBEGF) were expressed by conceptus and endometrium were attributed to cross-talk. In addition, Cytoscape interaction analysis revealed a plethora of interactions between genes expressed by the conceptus and endometrium, during a period when the former had substantial movement within the uterus. This is the first report of concurrent transcriptome analysis of conceptus and endometrium in the mare, with numerous findings to provide rationale for further investigation.

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