scholarly journals The Protein Coded by a Short Open Reading Frame, Not by the Annotated Coding Sequence, Is the Main Gene Product of the Dual-Coding GeneMIEF1

2018 ◽  
Vol 17 (12) ◽  
pp. 2402-2411 ◽  
Author(s):  
Vivian Delcourt ◽  
Mylène Brunelle ◽  
Annie V. Roy ◽  
Jean-François Jacques ◽  
Michel Salzet ◽  
...  
Keyword(s):  
Gene Product ◽  
Open Reading Frame ◽  
Dual Coding ◽  
Reading Frame ◽  
Coding Sequence ◽  
Short Open Reading Frame ◽  
Main Gene

scholarly journals The Immediate-Early Gene Product Encoded by Open Reading Frame 57 of Herpesvirus Saimiri Modulates Gene Expression at a Posttranscriptional Level

1998 ◽  
Vol 72 (1) ◽  
pp. 857-861 ◽  
Author(s):  
Adrian Whitehouse ◽  
Matthew Cooper ◽  
David M. Meredith
Keyword(s):  
Gene Expression ◽  
Gene Product ◽  
Target Gene ◽  
Immediate Early Gene ◽  
Open Reading Frame ◽  
Early Gene ◽  
Immediate Early ◽  
Herpesvirus Saimiri ◽  
Amino Acid Homology ◽  
Reading Frame

ABSTRACT The herpesvirus saimiri (HVS) immediate-early gene product encoded by open reading frame (ORF) 57 shares limited amino acid homology with HSV-1 ICP27 and Epstein-Barr virus BMLF1, both regulatory proteins. The ORF 57 gene has been proposed to be spliced based on the genome sequence, and here we confirm the intron-exon structure of the gene. We also demonstrate that a cDNA construct of the ORF 57 gene product represses the transactivating capability of the ORF 50a gene product (which is produced from a spliced transcript), but activates that of ORF 50b (an unspliced transcript). Further analyses with cotransfection experiments show that ORF 57 can either activate or repress expression from a range of both early and late HVS promoters, depending on the target gene. These results indicate that repression of gene expression mediated by the ORF 57 gene product is dependent on the presence of an intron within the target gene encoding region. Furthermore, Northern blot analysis demonstrates that the levels of mRNA transcribed from genes not containing an intron are not significantly affected in the presence of the ORF 57 gene product. This suggests that it regulates gene expression through a posttranscriptional mechanism.

scholarly journals A 5′-upstream short open reading frame encoded peptide regulates angiotensin type 1a receptor production and signalling via the β-arrestin pathway

2015 ◽  
Vol 594 (6) ◽  
pp. 1601-1605 ◽  
Author(s):  
Gina L.C. Yosten ◽  
Jun Liu ◽  
Hong Ji ◽  
Kathryn Sandberg ◽  
Robert Speth ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Reading Frame ◽  
Short Open Reading Frame ◽  
Angiotensin Type

scholarly journals Identification of the ubiD Gene on theEscherichia coli Chromosome

2000 ◽  
Vol 182 (21) ◽  
pp. 6243-6246 ◽  
Author(s):  
Haitao Zhang ◽  
George T. Javor
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Gene Product ◽  
Mutant Strain ◽  
Amino Acid Residue ◽  
Open Reading Frame ◽  
Reading Frame

ABSTRACT The open reading frame at 86.7 min on the Escherichia coli chromosome, “yigC,” complemented aubiD mutant strain, AN66, indicating that yigCis the ubiD gene. The gene product, a 497-amino-acid-residue protein, showed extensive homology to the UPF 00096 family of proteins in the Swiss-Prot database.

scholarly journals A Short Open Reading Frame Encompassing the MicroRNA173 Target Site Plays a Role in trans-Acting Small Interfering RNA Biogenesis

2016 ◽  
Vol 171 (1) ◽  
pp. 359-368 ◽  
Author(s):  
Manabu Yoshikawa ◽  
Taichiro Iki ◽  
Hisataka Numa ◽  
Kyoko Miyashita ◽  
Tetsuo Meshi ◽  
...  
Keyword(s):  
Small Interfering Rna ◽  
Target Site ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Short Open Reading Frame ◽  
Rna Biogenesis ◽  
In Trans ◽  
Interfering Rna

scholarly journals Tn2009, a Tn916-Like Element Containing mef(E) in Streptococcus pneumoniae

2004 ◽  
Vol 48 (6) ◽  
pp. 2037-2042 ◽  
Author(s):  
Maria Del Grosso ◽  
Anna Scotto d'Abusco ◽  
Francesco Iannelli ◽  
Gianni Pozzi ◽  
Annalisa Pantosti
Keyword(s):  
Streptococcus Pneumoniae ◽  
Insertion Site ◽  
Open Reading Frame ◽  
Genetic Element ◽  
M Gene ◽  
Reading Frame ◽  
Coding Sequence ◽  
Clinical Strains ◽  
Composite Element ◽  
Pneumococcal Strain

ABSTRACT The association between the macrolide efflux gene mef(E) and the tet(M) gene was studied in two clinical strains of Streptococcus pneumoniae that belonged to serotypes 19F and 6A, respectively, and that were resistant to both tetracycline and erythromycin. The mef(E)-carrying element mega (macrolide efflux genetic assembly; 5,511 bp) was found to be inserted into a Tn916-like genetic element present in the chromosomes of the two pneumococcal strains. In both strains, mega was integrated at the same site, an open reading frame identical to orf6 of Tn916. The new composite element, Tn2009, was about 23.5 kb and, with the exception of the tet(M)-coding sequence, appeared to be identical in both strains. By sequencing of the junction fragments of Tn2009 at the site of insertion into the chromosome, it was possible to show that (i) the insertion site was identical in the two clinical strains and (ii) the integration of Tn2009 caused a 9.5 kb-deletion in the pneumococcal chromosome. It was not possible to detect the conjugal transfer of Tn2009 to a recipient pneumococcal strain; however, transfer of the whole element by transformation was shown to occur. It is possible to hypothesize that Tn2009 relies on transformation for its spread among clinical strains of S. pneumoniae.

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