scholarly journals The effect of reproduction on the interaction of dietary protein and calcium

1966 ◽  
Vol 20 (4) ◽  
pp. 733-745 ◽  
Author(s):  
N. R. H. El-Maraghi ◽  
B. S. Plantt ◽  
R. J. C. Stewart

1. Rats were maintained from the age of 3 weeks until the end of a second reproductive cycle on diets having different protein values and Ca concentrations. 2. Reproductive performance was judged from the number of viable young, their size, body composition and rate of growth during suckling. 3. The size and quality of the bones of the mothers and offspring were assessed from radiographs, histological appearance, total weight of ash, ash/cm3 and ash:organic matter ratios of dry fat-free bone. 4. At the end of gestation the mean numbers and total weights of foetuses were low when the mothers had received diets of low protein value: there were smaller differences in the body-weight, water, ash and N contents of the individual foetuses. 5. When 0.44% Ca was included in the diet of low protein value, only one (three rats) of the six litters born survived beyond 1 week of age. 6. At weaning, the young born of and suckled by the protein-calorie deficient dams were only about half the weight of those from mothers receiving the high-protein diets; Ca deficiency produced relatively minor changes. 7. All the mothers lost weight during lactation irrespective of the protein value or Ca concentration of the diet; their bones had lower radiographic densities and less ash/cm3 than is usual in non-pregnant rats of similar age. 8. The interaction of protein and Ca and their relative importance in maintaining the skeletal structure of mother and offspring during pregnancy and lactation are discussed.

2003 ◽  
Vol 128 (1) ◽  
pp. 17-26 ◽  
Author(s):  
David J. Kay ◽  
Richard M. Rosenfeld

OBJECTIVE: The goal was to validate the SN-5 survey as a measure of longitudinal change in health-related quality of life (HRQoL) for children with persistent sinonasal symptoms. DESIGN AND SETTING: We conducted a before and after study of 85 children aged 2 to 12 years in a metropolitan pediatric otolaryngology practice. Caregivers completed the SN-5 survey at entry and at least 4 weeks later. The survey included 5 symptom-cluster items covering the domains of sinus infection, nasal obstruction, allergy symptoms, emotional distress, and activity limitations. RESULTS: Good test-retest reliability ( R = 0.70) was obtained for the overall SN-5 score and the individual survey items ( R ≥ 0.58). The mean baseline SN-5 score was 3.8 (SD, 1.0) of a maximum of 7.0, with higher scores indicating poorer HRQoL. All SN-5 items had adequate correlation ( R ≥ 0.36) with external constructs. The mean change in SN-5 score after routine clinical care was 0.88 (SD, 1.19) with an effect size of 0.74 indicating good responsiveness to longitudinal change. The change scores correlated appropriately with changes in related external constructs ( R ≥ 0.42). CONCLUSIONS: The SN-5 is a valid, reliable, and responsive measure of HRQoL for children with persistent sinonasal symptoms, suitable for use in outcomes studies and routine clinical care.


2020 ◽  
Vol 4 (4) ◽  
pp. 178-183
Author(s):  
M. Scavone ◽  
C. Gizzi ◽  
E. Albi

AbstractEndometriosis is a common condition that affects reproductive-aged women and is characterized by the presence of endo-metrial tissue outside the uterine cavity with nodules and lesions. The study aimed to analyze lifestyles of women affected by endometriosis in relation with their symptoms. In this observational study, 735 Italian women have been interviewed online through a questionnaire structured in two parts. The first part was mainly anamnestic, focused on gathering information about the age, the stage of disease, comorbidities, involved organs, symptomatology, chirurgical treatment, induced menopause. The second part focused on the individual characteristics and lifestyle of the patients such as the body mass index, physical activity, quality of sleep, and the diet. The results showed how a healthy diet and a regular physical activity reduce drastically the symptoms of the patients.


2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Weiwei Lin

Objective (1)Through the blood physiological and biochemical tests during the altitude training, to analyze the body function of swimmers in this stage.(2) Through the individual lactate threshold tests before and after the altitude training,to analyze the effects of altitude training. Methods Eight swimmers took a 26-day altitude training session.The individual lactate threshold test was carried out by the Swedish Monak839E power cycle progressive loading method before and after the training;During the altitude training period, 5ml of the subjects' elbow vein was extracted and tested on an empty stomach and in a quiet state every Monday morning. Results (1)When swimmers reached the plateau, the hemoglobin value was indistinguishable from the plain(male 156.2±7.01,female 135.7±8.75g/L),From the hemoglobin value (male 154.03 + 5.67, female 134.23 + 9.66g/L), there was a decrease in both male and female in the second week.But hypoxia stimulated red blood cell production, and the body itself was gradually adapting to the training load.Thus, the hemoglobin value of the third week (male 157.17 + 3.7, female 141.93 + 10.06g/L) was significantly improved, and higher than the level of the first week.During the altitude training period, the mean value of male’s blood testosterone was 474.33 + 97.06ng/dl, and the female’s blood testosterone was 33.67 + 17.25ng/dl.Male’s blood testosterone was lower than the mean of the national team, because the study participants were youngers who were not fully developed and had shorter training years.There were different trends in blood testosterone value between male and female. Male’s blood testosterone values during the Monday morning of these three weeks were 479.67±76.25、492.33±83.61、451±153.41ng/dl respectively.female’s blood testosterone values during the Monday morning of these three weeks were 29.33±21.83、32±23.26、39.67±9.29ng/dl respectively.These further indicated that this altitude training plan was more suitable for male with shorter training years, and the body had certain fatigue accumulation, but the decrease range was within a reasonable range.However, the increase of blood testosterone per week in female indicated that the training stimulation depth was not enough, and the potential of athletes should be further explored.According to the changes of creatine kinase, the sensitivity of male to the change of altitude training intensity was also shown, and the highest value of creatine kinase was 731U/L in the first week.(2) From the value of the individual lactate threshold before and after altitude training, no matter male or female,the change was not obvious, but was generally improved, this may be the altitude training adopted the pattern of three and a half weeks, training time was short.Secondly, as a professional athlete, the "plastic space" gradually decreased with the extension of the training years.Most of the peak blood lactate occurred in 1-3 minutes of recovery period.and the average value increased from 8.96 + 1.86mmol/L before altitude training to 9.99 + 1.47mmol/L.Among them, the peak value of male’s blood lactate was increased from 8 + 2.22mmol/L before the altitude training to 10.91 + 1.43mmol/L, and there was a significant difference in the peak of blood lactate before and after the altitude training.However, the peak value of female’s blood lactate was decreased from 9.92 + 0.79mmol/L before the altitude training to 9.07 + 0.88mmol/L. This was mainly due to the fact that a member of the swimmers had caused the result, and this swimmer’s enduring lactate level was lower than the one before the plateau. Conclusions The altitude training generally improved athletes’ training ability, but based on factors such as training age, gender, should be targeted according to the individual situation of each athlete training plan, so as to achieve more from less.


1991 ◽  
Vol 10 (2) ◽  
pp. 269-278 ◽  
Author(s):  
R. P. Beliles ◽  
N. G. Makris ◽  
W. J. Scott

A compartmental pharmacokinetic-mathematical model for the time-dependent distribution of hydroxyurea (HU) in both the maternal plasma and embryonic fluids of pregnant rats and rhesus monkeys was developed. Across species scaling was based on maternal plasma clearance rates and compartmental sizes as a percent of the body weight of the dam. Mathematical optimization provided the compartmental transfer rates. The estimated maternal and embryonic concentrations of HU correlated well with the experimental pharmacokinetic data regarding both time and quantity for both the rat and the monkey. When the biological effective dose was considered to be the embryonic HU concentration over time (AUC), the dose to the individual embryos was higher in the monkeys (392 mg HU hr/L/day) than in the rats (69 mg HU hr/L/day) at an applied dose of 100 mg HU/kg administered to the dams. The AUC doses are consistent with the evaluation of the embryos of both species for teratogenic changes and embryonic death. The effect of repeated doses as compared with a single dose given only on one day of gestation was examined in the rat. Because of the rapid maternal clearance the carryover of embryonic HU concentration from one day to the next was minimal. A single treatment on Day 9 only was estimated to be sufficient to produce the adverse embryonic effects of HU on Days 9 through 12 as reported by Wilson et al. The sensitivity of the embryo to HU decreased with increasing embryonic age. The implications of the pharmacokinetic simulation and the temporal susceptibility for ongoing clinical trials of HU in the treatment of sickle cell anemia were reviewed. A human embryo dose of 69 mg HU hr/L/day was estimated to result from an i.v. dose of 10 mg/kg to the mother. This concentration produced no effect in the rat. An i.v. dose of 50 mg HU/mg was estimated to result in a human embryo dose of 353 mg HU hr/L/day which approaches a Rhesus monkey embryo dose that produced adverse effects in all embryos.


Medicina ◽  
2019 ◽  
Vol 55 (9) ◽  
pp. 577 ◽  
Author(s):  
Viviane Gabriela Nascimento ◽  
Ciro João Bertoli ◽  
Paulo Rogerio Gallo ◽  
Luiz Carlos de Abreu ◽  
Claudio Leone

Background and Objectives: To verify the use of the tri-ponderalmass index (TMI) as a screening tool for risk of central fat accumulation in preschool children. Materials and Methods: An observational, analytical study was carried out on samples from children 2 to 5 years of age. The body mass index (BMI) and the tri-ponderalmass index (TMI: Weight/height3) were calculated. The waist circumference-to-height ratio (WHtR) was used to classify central fat accumulation risk. Preschoolers whose WHtRwas in the upper tertile of the sample were classified as at risk for central fat accumulation. A comparison of the two indicators (BMI and TMI) was made from the area under the receiver operator characteristics (ROC) curve (AUC) in the discrimination of the WHtR. Results: The sample used for analysis was 919 preschoolers. The mean age of the children was 3.9 years (SD = 0.7). The difference in AUC was 5% higher for TMI (p < 0.0001). In the individual analysis of the ROC curve of the TMI, favoring a higher sensitivity, the cutoff point of 14.0 kg/m3 showed a sensitivity of 99.3% (95% CI: 97.6–99.9). Conclusion: Considering WHtR as a marker of possible future metabolic risk among preschool children, TMI proved to be a useful tool, superior to BMI, in screening for risk of central fat accumulation in preschool children.


Author(s):  
Lon J. Van Winkle ◽  
Vasiliy Galat ◽  
Philip M. Iannaccone

The conversion of lysine to glutamate is needed for signaling in all plants and animals. In mouse embryonic stem (mES) cells, and probably their progenitors, endogenous glutamate production and signaling help maintain cellular pluripotency and proliferation, although the source of glutamate is yet to be determined. If the source of glutamate is lysine, then lysine deprivation caused by maternal low-protein diets could alter early embryo development and, consequently, the health of the offspring in adulthood. For these reasons, we measured three pertinent variables in human embryonic stem (hES) cells as a model for the inner cell masses of human blastocysts. We found that RNA encoding the alpha-aminoadipic semialdehyde synthase enzyme, which regulates glutamate production from lysine, was highly expressed in hES cells. Moreover, the mean amount of lysine consumed by hES cells was 50% greater than the mean amount of glutamate they produced, indicating that lysine is likely converted to glutamate in these cells. Finally, hES cells expressed RNA encoding at least two glutamate receptors. Since this may also be the case for hES progenitor cells in blastocysts, further studies are warranted to verify the presence of this signaling process in hES cells and to determine whether lysine deprivation alters early mammalian embryo development.


2019 ◽  
Vol 20 (3) ◽  
pp. 469 ◽  
Author(s):  
Marilia Martins Cavariani ◽  
Talita de Mello Santos ◽  
Dhrielly Natalia Pereira ◽  
Luiz Gustavo de Almeida Chuffa ◽  
Patricia Fernanda Felipe Pinheiro ◽  
...  

Background: Maternal protein restriction causes sperm alterations in the offspring, most of which are associated with epididymal functions. Because fluid reabsorption/secretion dynamics in the epididymal environment play important roles in the process of sperm maturation and concentration, we investigated the effects of maternal protein restriction on the expression of aquaporins (AQP1 and AQP9), vascular endothelial growth factor (VEGFa), and its receptor VEGFr-2 in different stages of postnatal epididymal development. Methods: Pregnant rats were divided into groups that received normoprotein (17% protein) and low-protein diets (6% protein) during gestation and lactation. After weaning, male rats only received the standard diet and were euthanized at the predetermined ages of 21, 44 and 120 days. Results: Maternal protein restriction decreased AQP1 and AQP9 expression in the initial segment and caput epididymis compared to the increased expression of these proteins observed in the corpus and cauda at all ages. Although protein restriction reduced the microvasculature density (MVD) on postnatal day (PND) 21 and 44, the MVD was unaltered on PND 120. Conclusions: Maternal protein restriction changed the structure or function of the offspring’s epididymis, specifically by affecting fluid dynamics and vasculogenesis in important stages of epididymis development.


1975 ◽  
Vol 228 (4) ◽  
pp. 1284-1287 ◽  
Author(s):  
PM Leung ◽  
BA Horwitz

Infusion of bacterial pyrogen (Priomen) was accompanied by an increase in body temperature, an increase in heat production, and a decrease in the voluntary food intake ofrats fed high-as well as low-protein diets. The magnitude of this pyrogen-induced depression of food intake was comparable for both diets. However, in rats fed high-protein diets, this decrease was additive to that normally seen following administration of such diets. These data indicate that the control of food intake cannot be explained in terms of a behavioral the more regulatory response.


2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16731-e16731
Author(s):  
Mariacristina Di Marco ◽  
Claudio Ricci ◽  
Riccardo Carloni ◽  
Elisa Grassi ◽  
Stefania De Lorenzo ◽  
...  

e16731 Background: Locally advanced pancreatic cancer (LAPC) is usually treated with chemoradiotherapy with poor results, thus additional therapies have been proposed. Of the latter, electrochemotherapy (ECT) represents a non-thermal ablation method, which combines the administration of chemotherapeutic drugs with permeabilizing electric pulses for cell membrane electroporation. The present study is the first to assess the short and long-term results, and the quality of life of the patients who underwent ECT for LAPC. Methods: Observational study of patients affected by LAPC who underwent intraoperative ECT after chemoradiotherapy. The inclusion criteria were: 1- patients with LAPC (defined according to the National Comprehensive Cancer Network 2019), 2- previous chemoradiotherapy and 3- absence of disease progression at restaging. Data at diagnosis and at restaging were collected for each patient. The Quality of life was evaluated using the Euro Quality of Life Group Association Questionnaire (EQ-5D-5L). The questionnaire was administered to all patients before and after ECT. Results: From May 25, 2018 to November 26, 2019 five patients underwent ECT: in 4 cases, the tumors were located in the head and, in one, in the body of the pancreas. Preoperative chemotherapy consisted mainly of 6 cycles of modified folfirinox, while the radiotherapy consisted of 54 Gy (27 fractions). At restaging, the serum value of CA 19-9 and tumor size were reduced; however, the vascular involvement did not change. No downstaging was recorded. Intravenous bleomycin 15,000IU/m2 was given as a bolus, the ECT procedure was performed using at least 4 needles with a mean duration time of 27 minutes, (range 15-40). No postoperative mortality or major complications were reported. The mean length of stay was 8 days (range 5-14). Four patients were alive and well at the end of the study while one patient died from disease progression. The mean follow-up was 20.8 months (range 9-34) from diagnosis and 9.4 months (range 2-19) from ECT. The quality of life was good (EQ-5D-5L scale > 50 in all cases) and there was improvement in pain/discomfort with respect to the pre-treatment period in 3 out of 5 patients. Conclusions: Electrochemotherapy can be considered a simple, feasible and safe palliative additional treatment in LAPC without progression after chemoradiotherapy, and it seems to allow a good quality of life and pain improvement.


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