Modulation of Cell Growth and Matrix Metalloproteinase-2 Activation of Oral Squamous Cell Carcinoma as a Function of Culture Condition with Type I Collagen

2003 ◽  
Vol 123 (8) ◽  
pp. 987-993 ◽  
Author(s):  
Hidenori Kinsenn ◽  
Hiroshi Sato ◽  
Mitsuru Furukawa ◽  
Tomokazu Yoshizaki
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Growth ◽  
Oral Squamous Cell Carcinoma ◽  
Matrix Metalloproteinase ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Culture Condition ◽  
Type I Collagen ◽  
Matrix Metalloproteinase 2 ◽  
Type I

scholarly journals Molecular docking of alkaloid compounds with the matrix metalloproteinase 2

2021 ◽  
Vol 17 (1) ◽  
pp. 206-211
Author(s):  
JH Shazia Fathima ◽  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Molecular Docking ◽  
Oral Squamous Cell Carcinoma ◽  
Matrix Metalloproteinase ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Aristolochic Acid ◽  
Matrix Metalloproteinase 2 ◽  
The Matrix

Matrix metalloproteinase protein-2 (MMP-2) is linked to the human oral squamous cell carcinoma. Therefore, it is of interest to design new inhibitors for MMP-2 to combat the disease. Thus, we document the molecular docking features of Aristolochic acid, Cryptopleurine, Epipodophyllotoxin, and Fagaronine with MMP-2 for further consideration.

scholarly journals Cancer-associated fibroblasts promote oral squamous cell carcinoma progression through LOX-mediated matrix stiffness

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Jia-Yi Zhang ◽  
Wei-Wen Zhu ◽  
Meng-Yao Wang ◽  
Run-Dong Zhai ◽  
Qiong Wang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Type I Collagen ◽  
Morphological Changes ◽  
Clinicopathological Parameters ◽  
Type I ◽  
Cancer Associated Fibroblasts ◽  
Matrix Stiffness

Abstract Background Cancer-associated fibroblasts (CAFs), the most abundant cells in the tumor microenvironment, have prominent roles in the development of solid tumors as stromal targets. However, the underlying mechanism of CAFs’ function in oral squamous cell carcinoma (OSCC) development remains unclear. Here, we investigated the role of lysyl oxidase (LOX) expression in CAFs in tumor stromal remodeling and the mechanism of its effect on OSCC progression. Methods Multiple immunohistochemistry (IHC) staining was performed to detect the correlation of CAFs and LOX in the stroma of OSCC specimens, as well as the correlation with clinicopathological parameters and prognosis. The expression of LOX in CAFs were detected by RT-qPCR and western blot. The effects of LOX in CAFs on the biological characteristics of OSCC cell line were investigated using CCK-8, wound-healing and transwell assay. CAFs were co-cultured with type I collagen in vitro, and collagen contraction test, microstructure observation and rheometer were used to detect the effect of CAFs on remodeling collagen matrix. Then, collagen with different stiffness were established to investigate the effect of matrix stiffness on the progression of OSCC. Moreover, we used focal adhesion kinase (FAK) phosphorylation inhibitors to explored whether the increase in matrix stiffness promote the progression of OSCC through activating FAK phosphorylation pathway. Results LOX was colocalized with CAFs in the stroma of OSCC tissues, and its expression was significantly related to the degree of malignant differentiation and poor prognosis in OSCC. LOX was highly expressed in CAFs, and its knockdown impaired the proliferation, migration, invasion and EMT process of OSCC cells. The expression of LOX in CAFs can catalyze collagen crosslinking and increase matrix stiffness. Furthermore, CAFs-derived LOX-mediated increase in collagen stiffness induced morphological changes and promoted invasion and EMT process in OSCC cells by activating FAK phosphorylation pathway. Conclusions Our findings suggest that CAFs highly express LOX in the stroma of OSCC and can remodel the matrix collagen microenvironment, and the increase in matrix stiffness mediated by CAFs-derived LOX promotes OSCC development through FAK phosphorylation pathway. Thus, LOX may be a potential target for the early diagnosis and therapeutic treatment of OSCC.

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