Changes of Glucose Oxidase Activity and Amount of Gluconic Acid Formation in the Hypopharyngeal Glands during the Lifespan of Honey Bee Workers (Apis melliferal.)

1990 ◽  
Vol 54 (8) ◽  
pp. 2133-2134 ◽  
Author(s):  
Tetsuo Takenaka ◽  
Hiro Ito ◽  
Kazuhisa Yatsunami ◽  
Takashi Echigo
Keyword(s):  
Glucose Oxidase ◽  
Honey Bee ◽  
Oxidase Activity ◽  
Gluconic Acid ◽  
Acid Formation ◽  
Hypopharyngeal Glands ◽  
Honey Bee Workers

scholarly journals Antimicrobial activity of fifteen Italian honeys against Paenibacillus larvae ATCC 9545

2018 ◽  
Vol 68 (4) ◽  
pp. 547 ◽  
Author(s):  
S. SAGONA ◽  
B. TURCHI ◽  
F. FRATINI ◽  
M. GIUSTI ◽  
B. TORRACCA ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Glucose Oxidase ◽  
Inhibitory Activity ◽  
Oxidase Activity ◽  
Gluconic Acid ◽  
Total Flavonoid ◽  
Paenibacillus Larvae ◽  
Economic Losses ◽  
Total Phenolic ◽  
Honey Sample

Currently, American Foulbrood (AFB) represents one of the most important problems for beekeepers, due to economic losses and to the absence of an effective therapeutic treatment. The aim of this work was to characterize fifteen Italian honeys in order to assess their inhibitory activity against Paenibacillus larvae ATCC 9545. Each honey was analyzed for the activity of the following enzymes: glucose oxidase and catalase. Moreover, melissopalynological analysis and other biochemical parameters, namely gluconic acid, total phenolic and total flavonoid contents were determined. For each honey, the Minimum Inhibitory Concentration (M.I.C.) and the Minimum Bactericidal Concentration (M.B.C.) against P. larvae were determined. All tested honey samples had an inhibitory activity on P. larvae. In particular, the lowest M.I.C. and M.B.C. values (53.8 mg/mL and 107.5 mg/mL, respectively) were recorded for an Arbutus honey sample. Arbutus honeys also had the highest gluconic acid and total phenolic contents (12.6 ± 1.7 g/kg and 243.2 ± 25.1 mg/kg, respectively) and the highest glucose oxidase activity (13.0 ± 1.9 nM H2O2/min). Dark honeys, including Arbutus, seem to have a higher gluconic acid content and a higher antimicrobial activity. Thus, honey characterization, including colour and physico-chemical characteristics (e.g. gluconic acid concentration, total phenolic and total flavonoid contents, glucose oxidase activity), could be crucial for the assessment of its employment against P. larvae.

scholarly journals Nutritional benefit of fungal spores for honey bee workers

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Jorgiane B. Parish ◽  
Eileen S. Scott ◽  
Katja Hogendoorn
Keyword(s):  
Honey Bee ◽  
Honey Bees ◽  
Nutritional Value ◽  
Fungal Spores ◽  
Fungal Spore ◽  
Poor Quality ◽  
Colletotrichum Acutatum ◽  
Hypopharyngeal Glands ◽  
Ovarian Activation ◽  
Honey Bee Workers

Abstract The collection of fungal spores by honey bees, Apis mellifera, can be classified as active or passive, the latter when spores are associated with pollen, nectar or honey dew. While low quality and shortage of pollen have been raised as hypotheses for fungal spore collection, the nutritional value of fungal spores for honey bees is poorly understood. Here we investigated the effect of consumption of fungal spores on survival, ovarian activation and the development of the hypopharyngeal glands (HPGs) in honey bee workers. Two pollen diets (Eucalyptus sp. pollen and a multifloral pollen) supplemented or not with spores of Botrytis cinerea, Cladosporium sp. or Colletotrichum acutatum were used. Consumption of diets that contained fungal spores increased the longevity of honey bee workers but had no significant effect on the development of their HPGs and ovaries. This demonstrates that fungal spores may have nutritional value for honey bees and that the consumption of fungal spores may compensate for nutritional imbalances of poor-quality pollen diets.

Cytotoxic effects on the midgut, hypopharyngeal, glands and brain of Apis mellifera honey bee workers exposed to chronic concentrations of lambda-cyhalothrin

Chemosphere ◽  
2020 ◽  
Vol 248 ◽  
pp. 126075 ◽  
Author(s):  
Mayara Badaró Arthidoro de Castro ◽  
Luis Carlos Martinez ◽  
Jamile Fernanda Silva Cossolin ◽  
Raissa Santana Serra ◽  
José Eduardo Serrão
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Cytotoxic Effects ◽  
Hypopharyngeal Glands ◽  
Lambda Cyhalothrin ◽  
Honey Bee Workers

Evaluation of Trinder's Glucose Oxidase Method for Measuring Glucose in Serum and Urine

1975 ◽  
Vol 21 (12) ◽  
pp. 1754-1760 ◽  
Author(s):  
John A Lott ◽  
Kathie Turner
Keyword(s):  
Glucose Oxidase ◽  
Oxidase Activity ◽  
Color Reaction ◽  
Critical Variable ◽  
Wide Range ◽  
Serum And Urine ◽  
Good Agreement

Abstract Trinder's method for glucose has nearly all the attributes of an ideal automated colorimetric glucose oxidase procedure. The chemicals used in the color reaction with peroxidase are readily available, the solutions are stable and can be prepared by the user, the method is highly specific and largely free of interferences, the sensitivity can be adjusted by the user to cover a wide range of glucose concentrations, and the reagents are not hazardous. We found very good agreement between results by this method and by the hexokinase and Beckman Glucose Analyzer methods. The method has been modified and adapted to the AutoAnalyzer I and SMA 6/60 (Technicon) with manifolds that give very little interaction between specimens. A study of the method by the simplex technique revealed that the glucose oxidase activity in the reagent is the most critical variable.

Longevity of cross-fostered honey bee workers (Apis mellifera) of European and Africanized races

1981 ◽  
Vol 59 (8) ◽  
pp. 1571-1575 ◽  
Author(s):  
Mark L. Winston ◽  
Susan J. Katz
Keyword(s):  
Apis Mellifera ◽  
South America ◽  
Honey Bee ◽  
Race Differences ◽  
The Other ◽  
Partial Explanation ◽  
Africanized Bees ◽  
Honey Bee Workers

Longevities of two races of honey bee workers, European and Africanized, were compared both within colonies of their own races and within colonies of the other race. Differences in longevity were found which were likely due to inherited differences between workers of the two races. The age at which workers began foraging was one factor important in determining longevity. These results may provide a partial explanation for the success and impact of Africanized bees in South America, and also suggest that the northerly spread of these bees could be limited by worker life spans.

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