Age determination of minke whales (Balaenoptera acutorostrata) using the aspartic acid racemization technique

Sarsia ◽  
2002 ◽  
Vol 87 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Erik Olsen ◽  
Jan Sunde
Keyword(s):  
Aspartic Acid ◽  
Age Determination ◽  
Balaenoptera Acutorostrata ◽  
Aspartic Acid Racemization ◽  
Minke Whales

scholarly journals Two techniques of age estimation in cetaceans: GLGs in teeth and earplugs, and measuring the AAR rate in eye lens nucleus

2017 ◽  
Vol 10 ◽  
Author(s):  
Nynne H Nielsen ◽  
Gísli A. Víkingsson ◽  
Steen H. Hansen ◽  
Susanne Ditlevsen ◽  
Mads Peter Heide-Jørgensen
Keyword(s):  
Aspartic Acid ◽  
North Atlantic ◽  
Von Bertalanffy ◽  
Phocoena Phocoena ◽  
Growth Layer ◽  
Balaenoptera Acutorostrata ◽  
Aspartic Acid Racemization ◽  
Minke Whales ◽  
Fin Whales ◽  
Layer Groups

The ages of three species of cetaceans were estimated by counting the growth layer groups (GLG) and measuring the aspartic acid racemization rate (kAsp) by what is referred to as the Aspartic Acid Racemization (AAR) technique. Data on kAsp and the D/L ratio of aspartic acid at birth [(D/L)0] in North Atlantic common minke whales (Balaenoptera acutorostrata) are presented along with data on fin whales (B. physalus) and harbour porpoises (Phocoena phocoena) already published by Nielsen et al. (2012). The kAsp specific for minke whales was 1.40 x 10-3 yr-1 (SE ± 0.00005) and the (D/L)0 was 0.0194 (SE ± 0.0012). The correlation of GLG age and D/L ratio for all three species was highly significant; however, the correlation coefficient varied greatly (fin whales: R2 = 0.59, p <0.0001; minke whales: ­R2=0.96, P <0.0001; harbour porpoises: ­R2=0.36, P <0.0001). Asymptotic body length for all three species was estimated by a von Bertalanffy growth model on both the GLG and AAR techniques, and showed no difference.

scholarly journals Age estimation of Antarctic minke whales Balaenoptera bonaerensis based on aspartic acid racemization technique

2017 ◽  
Vol 83 (6) ◽  
pp. 947-954
Author(s):  
Genta Yasunaga ◽  
Luis A. Pastene ◽  
Takeharu Bando ◽  
Takashi Hakamada ◽  
Yoshihiro Fujise
Keyword(s):  
Aspartic Acid ◽  
Age Estimation ◽  
Aspartic Acid Racemization ◽  
Minke Whales ◽  
Balaenoptera Bonaerensis

A modified HPLC method for the determination of aspartic acid racemization in collagen from human dentin and its comparison with GC

2004 ◽  
Vol 27 (4) ◽  
pp. 330-334 ◽  
Author(s):  
Terezie Benešová ◽  
Aleš Honzátko ◽  
Alexandr Pilin ◽  
Jaroslav Votruba ◽  
Miroslav Flieger
Keyword(s):  
Aspartic Acid ◽  
Hplc Method ◽  
Aspartic Acid Racemization ◽  
Human Dentin

A proposed technique for quantifying muscle progesterone content in minke whales (Balaenoptera acutorostrata)

1994 ◽  
Vol 72 (2) ◽  
pp. 368-370 ◽  
Author(s):  
Motoi Yoshioka ◽  
Takuji Okumura ◽  
Katsumi Aida ◽  
Yoshihiro Fujise
Keyword(s):  
Serum Levels ◽  
Balaenoptera Acutorostrata ◽  
Minke Whales ◽  
Quantitative Extraction ◽  
A New Technique ◽  
Ethanol Extracts ◽  
Free Ranging ◽  
Near Future ◽  
Muscle Biopsies

We have developed a new technique for the quantitative extraction of progesterone from very small pieces of muscle tissue of minke whales (Balaenoptera acutorostrata) post mortem. Ethanol extracts of approximately 0.5 g of tissue contained, on average, 15.3 ng/g progesterone in the case of pregnant females (SD = 12.7, n = 9) and 0.32 ng/g progesterone in the case of immature females (SD = 0.03, n = 9). Differences in muscle progesterone levels between females in the two reproductive states were significant, as has been previously reported also for serum levels. Our technique is expected to be applicable in the near future to the determination of the presence or absence of the corpus luteum in the ovaries and the reproductive status of free-ranging female whales, provided that sampling techniques for muscle biopsies are developed.

DETERMINATION OF PREGNANCY STATUS FROM BLUBBER SAMPLES IN MINKE WHALES (BALAENOPTERA ACUTOROSTRATA)

2002 ◽  
Vol 18 (1) ◽  
pp. 112-120 ◽  
Author(s):  
Atef A. H. Mansour ◽  
Donald W. Mkay ◽  
Jon Lien ◽  
James C. Orr ◽  
Joseph H. Banoub ◽  
...  
Keyword(s):  
Balaenoptera Acutorostrata ◽  
Minke Whales ◽  
Pregnancy Status

Analysis of Fibronectin and TGF-β1 Immunoexpression to Determination of Wound Vitality in Wistar Rats (Rattus norvegicus)

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Nita Novita ◽  
Hasrayati Agustina ◽  
Bethy S. Hernowo ◽  
Abdul H. Hassan
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Age Determination ◽  
Scientific Field ◽  
Wound Age ◽  
Forensic Practice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tgf Β1

Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-β1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design.  The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-β1 (100%).  In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-β1.  In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-β1.  In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-β1.  In postmortem wound, all of samples showed negativity for fibronectin and TGF-β1. In conclusion, fibronectin and TGF-β1 may be useful in the determination of wound vitality. Keywords: wound, fibronectin, TGF-β1, vitality

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