Spleen immunotoxicities induced by intra-testicular injection of magnetic nanoparticles and the role of Echinacea purpurea extract: a histological and immunohistochemical study

2018 ◽  
pp. 1-17
Author(s):  
Aziz Awaad ◽  
Mohamed A. Adly ◽  
Doaa Hosny
Keyword(s):  
Magnetic Nanoparticles ◽  
Immunohistochemical Study ◽  
Echinacea Purpurea ◽  
Testicular Injection ◽  
Echinacea Purpurea Extract

scholarly journals CD206+ macrophage is an accelerator of endometriotic-like lesion via promoting angiogenesis in the endometriosis mouse model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yosuke Ono ◽  
Osamu Yoshino ◽  
Takehiro Hiraoka ◽  
Erina Sato ◽  
Akiko Furue ◽  
...  
Keyword(s):  
Mouse Model ◽  
Diphtheria Toxin ◽  
Immunohistochemical Study ◽  
Mrna Levels ◽  
Cytokines And Chemokines ◽  
Diphtheria Toxin Receptor ◽  
Endothelial Cell Marker ◽  
Toxin Receptor ◽  
Group A

AbstractIn endometriosis, M2 MΦs are dominant in endometriotic lesions, but the actual role of M2 MΦ is unclear. CD206 positive (+) MΦ is classified in one of M2 type MΦs and are known to produce cytokines and chemokines. In the present study, we used CD206 diphtheria toxin receptor mice, which enable to deplete CD206+ cells with diphtheria toxin (DT) in an endometriosis mouse model. The depletion of CD206+ MΦ decreased the total weight of endometriotic-like lesions significantly (p < 0.05). In the endometriotic-like lesions in the DT group, a lower proliferation of endometriotic cells and the decrease of angiogenesis were observed. In the lesions, the mRNA levels of VEGFA and TGFβ1, angiogenic factors, in the DT group significantly decreased to approximately 50% and 30% of control, respectively. Immunohistochemical study revealed the expressions of VEGFA and an endothelial cell marker CD31 in lesions of the DT group, were dim compared to those in control. Also, the number of TGFβ1 expressing MΦ was significantly reduced compared to control. These data suggest that CD206+ MΦ promotes the formation of endometriotic-like lesions by inducing angiogenesis around the lesions.

scholarly journals The cypsela (achene) of Echinacea purpurea as a diffusion unit of a community of microorganisms

2021 ◽  
Vol 105 (7) ◽  
pp. 2951-2965
Author(s):  
Massimiliano Cardinale ◽  
Marian Viola ◽  
Elisangela Miceli ◽  
Teresa Faddetta ◽  
Anna Maria Puglia ◽  
...  
Keyword(s):  
Respiratory Infections ◽  
Active Role ◽  
Echinacea Purpurea ◽  
Endophytic Microorganisms ◽  
Host Membrane ◽  
Key Points ◽  
Endophytic Bacterial Community ◽  
Pharmaceutical Properties ◽  
Stimulation Of

AbstractEchinacea purpurea is a plant cultivated worldwide for its pharmaceutical properties, mainly related to the stimulation of the immune system in the treatment of respiratory infections. The cypselas (fruits) of E. purpurea were examined in order to investigate the presence, localization and potential function(s) of endophytic microorganisms. Electron and confocal microscopy observations showed that three different components of microorganisms were associated to cypselas of E. purpurea: (i) one endocellular bacterial component in the cotyledons, enclosed within the host membrane; (ii) another more generic bacterial component adhering to the external side of the perianth; and (iii) a fungal component inside the porous layer of the perianth, the woody and porous modified residual of the flower, in the form of numerous hyphae able to cross the wall between adjacent cells. Isolated bacteria were affiliated to the genera Paenibacillus, Pantoea, and Sanguibacter. Plate tests showed a general resistance to six different antibiotics and also to an antimicrobial-producing Rheinheimera sp. test strain. Finally, microbiome-deprived E. purpurea seeds showed a reduced ability to germinate, suggesting an active role of the microbiome in the plant vitality. Our results suggest that the endophytic bacterial community of E. purpurea, previously found in roots and stem/leaves, might be already carried at the seed stage, hosted by the cotyledons. A further microbial fungal component is transported together with the seed in the perianth of the cypsela, whose remarkable structure may be considered as an adaptation for fungal transportation, and could influence the capability of the seed to germinate in the soil.Key Points• The fruit of Echinacea purpurea contains fungi not causing any damage to the plant.• The seed cotyledons contain endocellular bacteria.• Seed/fruit deprived of the microbiome showed a reduced ability to germinate.

The role of ALK5 in the profile of early reproductive losses in the use of assisted reproductive technologies

2021 ◽  
pp. 70-76
Author(s):  
Abuduwaili Ruziguli ◽  
Nikolai Nikolaevich Rukhliada ◽  
Anna Nikolaevna Taits ◽  
Tatyana Ivanovna Prohorovich ◽  
Tatyana Aleksandrovna Libova
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
Embryo Transfer ◽  
Spontaneous Abortion ◽  
Assisted Reproductive Technologies ◽  
Immunohistochemical Study ◽  
Reproductive Technologies ◽  
Decidual Tissue ◽  
Reproductive Losses

This article is devoted to the assessment of the role of ALK5 in the profile of early reproductive losses in the use of assisted reproductive technologies, in particular, by using immunohistochemical study in the group of patients with early spontaneous abortion after the procedure of embryo transfer, a lower level of ALK5 expression in the decidual tissue was revealed (in comparison with control), which may be related to the occurrence of early reproductive losses caused by the imbalance in Th1 / Th2 and its effect on the increase in the concentration of natural killer cells.

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