Hepatoprotective effects of norgalanthamine on carbon tetrachloride induced-hepatotoxicity in mice

Background and Objective : A Tibetan traditional herb named Swertia mussotii Franch., also called “Zangyinchen” by the local people of Qinghai-Tibet area, has been used to protect the liver from injury for many years. However, the curative effect and molecular mechanism of the herb have not been demonstrated clearly. Materials and Methods: In our study, serum alanine aminotransferase, aspartate aminotransferase, total bilirubin levels were examined after S. mussotii Franch. treatment in the acute liver injury of the carbon tetrachloride-induced rat model. Then, Proteome Analysis was applied to explore the potential mechanism of SMT for hepatoprotective effects after iTRAQLC-MS/MS analysis (isobaric tag for relative and absolute quantification-liquid chromatograph-mass spectrometer with tandem mass spectrometry). Results: Serum results showed, alanine aminotransferase, aspartate aminotransferase, total bilirubin levels of rats with acute liver injury were all improved with SMT treatment. Moreover, Proteome Analysis suggested that, with S. Mussotii Franch. treatment, the levels of lipid catabolic process and lipid homeostasis were all enhanced. And the results of protein-protein interaction (PPI) analysis illustrated that these proteins assembled in PPI networks were found almost significantly enriched in response to lipid, negative regulation of lipase activity, response to lipopolysaccharide etc. Furthermore, the downregulated MRP14 and MRP8 proteins were found involved in the lipid metabolism, which may indicate the mechanism of SMT protection liver from ALI induced by carbon tetrachloride. Conclusion: SMT herb could play a role in hepatoprotection and alleviate the effect of acute liver injury by impacting the lipid metabolism associated biological process.

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Hepatoprotective effects of zingerone on carbon tetrachloride- and dimethylnitrosamine-induced liver injuries in rats

Archives of Pharmacal Research ◽

10.1007/s12272-015-0696-2 ◽

2015 ◽

Vol 39 (2) ◽

pp. 279-291 ◽

Cited By ~ 13

Author(s):

Kyoung Ook Cheong ◽

Dong-Su Shin ◽

Jeonghyeon Bak ◽

Changyong Lee ◽

Kyung Wook Kim ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injuries ◽

Hepatoprotective Effects

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Inhibition of cytochrome P450 2E1 expression by oleanolic acid: hepatoprotective effects against carbon tetrachloride-induced hepatic injury

Toxicology Letters ◽

10.1016/s0378-4274(99)00004-1 ◽

1999 ◽

Vol 105 (3) ◽

pp. 215-222 ◽

Cited By ~ 110

Author(s):

Hye Gwang Jeong

Keyword(s):

Cytochrome P450 ◽

Carbon Tetrachloride ◽

Oleanolic Acid ◽

Hepatic Injury ◽

Cytochrome P450 2E1 ◽

Hepatoprotective Effects

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Hepatoprotective Effects of Polysaccharides Isolated from Phellinus gilvus Against Carbon Tetrachloride-induced Liver Injury in Rats

Toxicological Research ◽

10.5487/tr.2009.25.1.029 ◽

2009 ◽

Vol 25 (1) ◽

pp. 29-33 ◽

Cited By ~ 2

Author(s):

Seung-Chun Park ◽

Yong-Pil Cheon ◽

Wha-Young Son ◽

Man-Hee Rhee ◽

Tae-Wan Kim ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Hepatoprotective Effects ◽

Phellinus Gilvus

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Hepatoprotective effects of hydroethanolic extracts of Crocus sativus tepals, stigmas and leaves on carbon tetrachloride induced acute liver injury in rats

Physiology and Pharmacology ◽

10.32598/ppj.25.2.30 ◽

2021 ◽

Vol 25 (2) ◽

pp. 178-188

Author(s):

Sabir Ouahhoud ◽

◽

Ilham Touiss ◽

Amine Khoulati ◽

Iliass Lahmass ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Injury ◽

Total Bilirubin ◽

Liver Weight ◽

Crocus Sativus ◽

Male Rats ◽

Control Group ◽

Distilled Water ◽

Relative Liver Weight ◽

Hepatoprotective Effects

Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and leaves of Crocus sativus on carbon tetrachloride (CCL4) induced liver injury in rats. Methods: Hydroethanolic extracts of Crocus sativus (stigmas, tepals and leaves) were administrated daily for 14 days by oral gavage. In the present study, 30 male rats divided into five groups were treated as 1: normal rats gavaged with distilled water; 2: intoxicated rats gavaged with distilled water and injected with CCL4; 3: rats treated with stigmas extract and injected with CCL4; 4: rats treated with tepal extract and injected with CCL4; 5: rats treated with leaf extract and injected with CCL4. Bodyweight and the relative liver weight were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol, triglycerides, bilirubin direct and total, total protein, albumin, urea and creatinine measured in plasma. Malondialdehyde (MDA) was quantified in liver homogenate. Results: The experimental data showed that the stigmas and tepals extracts significantly prevented weight body loss and improved the relative liver weight. They significantly protected against elevation of ALT, AST, direct bilirubin, total bilirubin, LDH, ALP, creatinine and MDA. Also, they enhanced significantly total proteins and albumin compared to the CCL4 control group. Moreover, leaves reduced ALT, AST, total bilirubin, LDH and MDA significantly. Conclusion: In conclusion, these results suggest that tepals, stigmas, and leaves extracts of Crocus sativus have hepatoprotective effects on CCL4 induced liver injury in rats.

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Camel Urotherapy and Hepatoprotective Effects Against Carbon Tetrachloride-induced Liver Toxicity

International Journal of Pharmacology ◽

10.3923/ijp.2019.696.705 ◽

2019 ◽

Vol 15 (6) ◽

pp. 696-705 ◽

Cited By ~ 1

Author(s):

Hany S. Mahmoud ◽

Wael M. Elsaed ◽

Sami A. Gabr

Keyword(s):

Carbon Tetrachloride ◽

Liver Toxicity ◽

Hepatoprotective Effects

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Hepatoprotective Effects of Saponins from Rhizoma panacis majoris on Hepatic Fibrosis Induced by Carbon Tetrachloride in Rats

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.568-570.1915 ◽

2014 ◽

Vol 568-570 ◽

pp. 1915-1920

Author(s):

Ji Hong Zhang ◽

Meng Qiong Shi ◽

Hai Bo He ◽

Cai Hong Bai ◽

Jun Zhi Wang ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Liver Function ◽

Antioxidant Capacity ◽

Hepatic Fibrosis ◽

Separation And Purification ◽

Sprague Dawley ◽

Gene Expressions ◽

Total Antioxidant ◽

Hepatoprotective Effects ◽

Underlying Mechanisms

Saponins from Rhizoma Panacis Majoris (SRPM), the bioactive component in Rhizoma Panacis Majoris, have been used extensively as a remedy for liver injury diseases and achieved good clinical efficacy, but the underlying mechanisms remain poorly understood. The goal of our present study was to further confirm SRPM hepatoprotective effect, and evaluate that whether SRPM attenuate oxidative stress and fibrosis in carbon tetrachloride (CCl4)-induced hepatic injury, based on these results, investigate the probable mechanisms involved. At first, the separation and purification of SRPM were studied. And then, in the animal experiment, the male Sprague-Dawley rats were randomly divided into control, model, l-SRPM and h-SRPM group. Hepatic fibrosis model were made according to our previous studies reported. At the same time, the experimental rats were treated respectively with relative drugs, once a day for 8 weeks. Hepatoprotective effects of SRPM were evaluated by liver function, total antioxidant capacity and total-superoxide dismutase, histopathological observations and the hepatic fibrosis relative gene expressions. In the study, we found that SRPM significantly improved liver function, serum antioxidation level, reversed the upregulated α-SMA and TIMP1 mRNA expressions, and further increased the MMP1 mRNA expression. Our studies indicated that SRPM exerted beneficially hepatoprotective effects on the CCl4 induced hepatic fibrosis, mainly enhancing liver tissue antioxidant capacity, reducing the lipid peroxidation of hepatocyte membranes, and then alleviating hepatic fibrosis and hepatic cell death.

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