Effects of bioflocculants on lipid extraction, fatty acid composition and reuse of the culture media for biodiesel production using Chlorella vulgaris

2020 ◽  
pp. 1-10
Author(s):  
Alenne Prince Junqueira de Moraes ◽  
Cláudia Maria Luz Lapa Teixeira ◽  
Adelia Ferreira Faria-Machado ◽  
Celso Luiz Salgueiro Lage
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Chlorella Vulgaris ◽  
Culture Media ◽  
Lipid Extraction ◽  
Biodiesel Production

Fetal bovine serum induces changes in fatty acid composition ofTrypanosoma cruziphosphoinositides

1995 ◽  
Vol 41 (10) ◽  
pp. 951-954 ◽  
Author(s):  
G. Racagni ◽  
M. G. de Lema ◽  
G. Hernández ◽  
E. E. Machado-Domenech
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Trypanosoma Cruzi ◽  
Acid Composition ◽  
Bovine Serum ◽  
Culture Medium ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

Fetal bovine serum (FBS) is a necessary constituent of the culture media employed to foster the growth of Trypanosoma cruzi epimastigote forms. In different laboratories, the serum is used at final concentrations of 5 or 10%. We have normally supplemented the complex medium with 10% FBS. Under this condition we have described the fatty acid composition of the total lipids and of the phosphoinositide fractions. Additionally, we have reported the increase of polyphosphoinositides and phosphatidic acid after cholinergic stimulation. Since further attempts to reproduce these results with 5% FBS in the culture medium were not successful, the effect of the FBS concentration on the fatty acid composition of phospholipids from the T. cruzi epimastigote forms was thoroughly examined. This work showed that when the FBS concentration supplementing the culture medium was reduced from 10 to 5%, the fatty acid composition of the phosphoinositides was altered while the other major phospholipids were not significantly affected. The most relevant result was the decrease in the content of linoleic acid (18:2) and the increase of palmitoleic acid (16:1) in phosphatidylinositol 4,5-bisphosphate. Phosphatidylinositol (PI) and phosphatidylinositol phosphate also exhibited similar changes in the same fatty acids. The C2fatty acid composition of the phosphoinositides, under the same conditions, is also reported here for the first time.Key words: Trypanosoma cruzi, fatty acids, phosphoinositides, fetal bovine serum, phospholipids.

UTILIZATION OF MILK AND ANIMAL FAT BY MICROORGANISMS – POTENTIAL PRODUCERS OF LIPASES AND ESTERASES

2020 ◽  
pp. 44-45
Author(s):  
D. Ivasenko ◽  
P. Bukhtiyarova ◽  
D. Antsiferov ◽  
Y. Frank
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Acid Composition ◽  
Liquid Culture ◽  
Culture Media ◽  
Bacterial Strains ◽  
Animal Fat ◽  
Pure Cultures

Analysis of fatty acid composition in liquid culture media after lipophilic bacterial strains cultivation was carried out. Pure cultures were earlierisolated from fat-containing wastes and cultivated on media with diverse fat sources. It was shown that microorganisms hydrolyze animal and milk fats to free fatty acids.

Changes in fatty acid composition of Chlorella vulgaris by hypochlorous acid

2014 ◽  
Vol 162 ◽  
pp. 379-383 ◽  
Author(s):  
Ji-Yeon Park ◽  
Sun-A Choi ◽  
Min-Ji Jeong ◽  
Bora Nam ◽  
You-Kwan Oh ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Chlorella Vulgaris ◽  
Hypochlorous Acid

scholarly journals Fatty acid profiles in adipose tissues and liver differ between horses and ponies

2018 ◽  
Author(s):  
Stephanie Adolph ◽  
Carola Schedlbauer ◽  
Dominique Blaue ◽  
Axel Schöniger ◽  
Claudia Gittel ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Liver Tissue ◽  
Lipid Extraction ◽  
Lipid Classes ◽  
Adipose Tissues ◽  
Health And Disease

AbstractFatty acids, as key components of cellular membranes and complex lipids, may play a central role in endocrine signalling and the function of adipose tissue and liver. Thus, the lipid fatty acid composition may play a role in health and disease status in the equine. This study aimed to investigate the fatty acid composition of different tissues and liver lipid classes by comparing Warmblood horses and Shetland ponies under defined conditions. We hypothesized that ponies show different lipid patterns than horses in adipose tissue, liver and plasma. Six Warmblood horses and six Shetland ponies were housed and fed under identical conditions. Tissue and blood sampling were performed following a standardized protocol. A one-step lipid extraction, methylation and trans-esterification method with subsequent gas chromatography was used to analyse the total lipid content and fatty acid profile of retroperitoneal, mesocolon and subcutaneous adipose tissue, liver and plasma. In the adipose tissues, saturated fatty acids (SFAs) and n-9 monounsaturated fatty acids (n-9 MUFAs) were most present in ponies and horses. N-6 polyunsaturated fatty acids (n-6 PUFAs), followed by SFAs, were most frequently found in liver tissue and plasma in all animals. Horses, in comparison to ponies, had significantly higher n-6 PUFA levels in all tissues and plasma. In liver tissue, horses had significantly lower hepatic iso-branched-chain fatty acids (iso-BCFAs) than ponies. The hepatic fatty acid composition of selected lipid classes was different between horses and ponies. In the polar PL fraction, horses had low n-9 MUFA and n-3 PUFA contents but higher n-6 PUFA contents than ponies. Furthermore, iso-BCFAs are absent in several hepatic lipid fractions of horses but not ponies. The differences in fatty acid lipid classes between horses and ponies provide key information on the species- and location-specific regulation of FA metabolism, thus affecting health and disease risk.

scholarly journals Integrated evaluation of seed oil composition and yield potential of oil radish as new high-productive biodiesel source

2018 ◽  
Vol 23 ◽  
pp. 24-30 ◽  
Author(s):  
R. Ya. Blume ◽  
G. V. Lantukh ◽  
O. V. Holubets ◽  
S. O. Rakhmetova ◽  
A. I. Yemets ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Chromatographic Analysis ◽  
Oil Content ◽  
Biodiesel Production ◽  
Aviation Fuel ◽  
Yield Potential ◽  
Oil Composition

Aim. Main aim of this research was a comparison of fatty acid composition for seed oils from oil radish breeding forms and varieties produced in Natl. Botanical Garden of Natl. Academy of Sciences of Ukraine. Methods. Biochemical analysis of oil content as well as chromatographic analysis of fatty acid composition of oil radish and its genotypes were conducted out. Results. Oil content in seeds of oil radish (Raphanus var. oleifera sativus L.) was determined. The highest oil content was indicated for variety Kyyanochka – 42 %. Basing on chromatographic analysis of fatty acids of oil radish genotypes most optimal fatty acid composition for biodiesel production was identified in Kyyanochka variety due to high content of short-chained and monounsaturated fatty acids and highest oleic (18:1) acid content – 37.89 %. Conclusions. Taking in account results of chromatographic analysis and agronomic productivity the best genotype for biodiesel production were identified: variety Kyyanochka. Chosen genotype could be used for production of light types of biofuel which have potential to be used as additive for aviation fuel according to the optimal fatty acid composition Keywords: Brassicaceae, oil radish, varieties, breeding forms, oil, fatty acids, biodiesel production.

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