Native Australian Fruit Polyphenols Inhibit Cell Viability and Induce Apoptosis in Human Cancer Cell Lines

We synthesized 2,3,6,7,10,11-hexahydroxytriphenylene (HHTP), characterized it by electrochemistry, spectroelectrochemistry, and electron paramagnetic resonance techniques, and evaluated its cytotoxicity to human cancer cell lines. The results revealed that HHTP has accessible higher-oxidation states, especially the tris-semiquinone monoradical. This species is stable and is formed after being stored for months. HHTP exhibited cytotoxic effects on 5 human cancer cell lines, including glioma and lung cancer cells. The cytotoxic effect was evaluated based on the decrease in cell viability, increases in the percentage of cells with fragmented DNA, and elevated numbers of annexin V–PI-positive cells after HHTP treatment.

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Abstract 1957: MicroRNA analysis paired with a novel live cell viability assay: a complete epigenetic workflow in human cancer cell lines

10.1158/1538-7445.am2016-1957 ◽

2016 ◽

Author(s):

Samantha Lewis ◽

Brad Hook ◽

Don Smith ◽

Douglas Horejsh ◽

Trista Schagat

Keyword(s):

Cell Viability ◽

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Lines ◽

Live Cell ◽

Human Cancer Cell ◽

Cell Viability Assay ◽

Human Cancer Cell Lines ◽

Viability Assay

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A Warp-Knitted Light-Emitting Fabric-Based Device for In Vitro Photodynamic Therapy: Description, Characterization, and Application on Human Cancer Cell Lines

Cancers ◽

10.3390/cancers13164109 ◽

2021 ◽

Vol 13 (16) ◽

pp. 4109

Author(s):

Elise Thécua ◽

Laurine Ziane ◽

Guillaume Paul Grolez ◽

Alexandre Fagart ◽

Abhishek Kumar ◽

...

Keyword(s):

Photodynamic Therapy ◽

Cell Viability ◽

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Lines ◽

Human Cancer Cell ◽

Light Emitting ◽

Human Cancer Cell Lines

Photodynamic therapy (PDT) appears to be a promising strategy in biomedical applications. However, the complexity of its parameters prevents wide acceptance. This work presents and characterizes a novel optical device based on knitted light-emitting fabrics and dedicated to in vitro PDT involving low irradiance over a long illumination period. Technical characterization of this device, called CELL-LEF, is performed. A cytotoxic study of 5-ALA-mediated PDT on human cancer cell lines is provided as a proof of concept. The target of delivering an irradiance of 1 mW/cm2 over 750 cm2 is achieved (mean: 0.99 mW/cm2; standard deviation: 0.13 mW/cm2). The device can maintain a stable temperature with the mean thermal distribution of 35.1 °C (min: 30.7 °C; max: 38.4 °C). In vitro outcomes show that 5-ALA PDT using CELL-LEF consistently and effectively induced a decrease in tumor cell viability: Almost all the HepG2 cells died after 80 min of illumination, while less than 60% of U87 cell viability remained. CELL-LEF is suitable for in vitro PDT involving low irradiance over a long illumination period.

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Comparison of the Usefulness of the MTT, ATP, and Calcein Assays to Predict the Potency of Cytotoxic Agents in Various Human Cancer Cell Lines

CrossRef Listing of Deleted DOIs ◽

10.1177/1087057104265386 ◽

2004 ◽

Vol 9 (6) ◽

pp. 506-515 ◽

Cited By ~ 144

Author(s):

Henrik Mueller ◽

Matthias U. Kassack ◽

Michael Wiese

Keyword(s):

Principal Component Analysis ◽

Cell Viability ◽

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Principal Component ◽

Component Analysis ◽

Cancer Cell Lines ◽

Human Cancer Cell ◽

Human Cancer Cell Lines

Cell viability assays are important tools in oncological research and clinical practice to assess the tumor cell sensitivity of individual patients. The purpose of this study was to demonstrate the comparability of 3 widely used assays (MTT, ATP, calcein assays) by principal component analysis. The study included 4 different cytostatics (cisplatin, docetaxel, doxorubicin, vinblastine) and 3 different human cancer cell lines (MCF-7, A2780, doxorubicin resistant A2780adr). Ninety-three percent of the total variance of all variables included in the principal component analysis (resulting from 3 cell lines and 3 assays) could be explained by 1 principal component. Factor loadings were > 0.937 except for the variable MTT-A2780adr, which was 0.872. These results indicate the similarity of the 3 assays. A 2nd principal component analysis included literature data and showed accordance of data from this study and the literature. The MTT assay was further improved as a high-throughput screening–capable assay. The ATP assay is able to detect effects of cytostatics already after 1 h incubation. The determination of resistance factors allowed to differentiate cytostatics into P-gp or non-P-gp substrates. In conclusion, this study provides improved microplate reader-based cell viability assays and sets a statistically solid basis for a future comparison of data obtained in different laboratories by any of the 3 assays.

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