scholarly journals Comparison of the immune responses against Salmonella enterica serovar Gallinarum infection between naked neck chickens and a commercial chicken line

2003 ◽  
Vol 32 (2) ◽  
pp. 193-203 ◽  
Author(s):  
María Teresa Alvarez ◽  
Nestor Ledesma ◽  
Guillermo Téllez ◽  
José Luis Molinari ◽  
Patricia Tato
Keyword(s):  
Immune Responses ◽  
Salmonella Enterica ◽  
Chicken Line ◽  
Naked Neck ◽  
Commercial Chicken

scholarly journals Acquiring Resistance Against a Retroviral Infection via CRISPR/Cas9 Targeted Genome Editing in a Commercial Chicken Line

2020 ◽  
Vol 2 ◽  
Author(s):  
Romina Hellmich ◽  
Hicham Sid ◽  
Kamila Lengyel ◽  
Krzysztof Flisikowski ◽  
Antonina Schlickenrieder ◽  
...  
Keyword(s):  
Genome Editing ◽  
Chicken Line ◽  
Retroviral Infection ◽  
Commercial Chicken

scholarly journals Immune Responses against Salmonella enterica Serovar Enteritidis Infection in Virally Immunosuppressed Chickens

2003 ◽  
Vol 10 (4) ◽  
pp. 670-679 ◽  
Author(s):  
Ruby R. Sheela ◽  
Uma Babu ◽  
Jie Mu ◽  
Subbiah Elankumaran ◽  
Daniel A. Bautista ◽  
...  
Keyword(s):  
Immune Responses ◽  
Salmonella Enterica ◽  
Immunoglobulin A ◽  
Chicken Anemia Virus ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Limited Effect ◽  
Field Situation ◽  
Infected Cells ◽  
Anemia Virus

ABSTRACT To understand the role of immune mechanisms in protecting chickens from Salmonella infections, we examined the immune responses of Salmonella enterica serovar Enteritidis-infected chickens and the effect of chicken anemia virus (CAV), a T-cell-targeted virus, on S. enterica serovar Enteritidis-induced immune responses. One-day-old chicks were orally inoculated with S. enterica serovar Enteritidis with or without intramuscular injection of CAV. The bacterial infection, pathology, and immune responses of chickens were evaluated at 14, 28, and 56 days postinoculation. The infection increased the levels of S. enterica serovar Enteritidis-specific mucosal immunoglobulin A (IgA), the number of gut-associated T cells, and the titer of serum IgG specific for S. enterica serovar Enteritidis surface antigens. CAV infection depressed these immune responses, especially the mucosal immune responses, but did not increase the number of S. enterica serovar Enteritidis-infected cells in the intestine. The severity of pathological lesions appeared to be reciprocal to the level of immune responses, but the S. enterica serovar Enteritidis infection persisted. These results suggest that oral infection of S. enterica serovar Enteritidis in chickens induces both mucosal and systemic immune responses, which have a limited effect on the S. enterica serovar Enteritidis infection under conditions designed to mimic the field situation.

scholarly journals T- and B-Cell Immune Responses of Patients Who Had Undergone Colectomies to Oral Administration of Salmonella enterica Serovar Typhi Ty21a Vaccine

2003 ◽  
Vol 10 (3) ◽  
pp. 426-430 ◽  
Author(s):  
Jan Kilhamn ◽  
Samuel B. Lundin ◽  
Hans Brevinge ◽  
Ann-Mari Svennerholm ◽  
Marianne Jertborn
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
B Cell ◽  
Healthy Volunteers ◽  
Vaccine Strain ◽  
Salmonella Enterica ◽  
Antibody Responses ◽  
Igg Antibody ◽  
Iga Antibody ◽  
Ifn Γ

ABSTRACT The capacity of an oral live attenuated Salmonella enterica serovar Typhi Ty21a vaccine to induce immune responses in patients who had undergone colectomies because of ulcerative colitis was evaluated, and these responses were compared with those of healthy volunteers. Purified CD4+ and CD8+ T cells from peripheral blood were stimulated in vitro by using the heat-killed Ty21a vaccine strain, and the proliferation and gamma interferon (IFN-γ) production were measured before and 7 or 8 days after vaccination. Salmonella-specific immunoglobulin A (IgA) and IgG antibody responses in serum along with IgA antibody responses in ileostomy fluids from the patients who had undergone colectomies were also evaluated. Three doses of vaccine given 2 days apart failed to induce proliferative T-cell responses in all the six patients who had undergone colectomies, and increases in IFN-γ production were found only among the CD8+ cells from three of the patients. In contrast, both proliferative responses and increased IFN-γ production were observed among CD4+ and CD8+ T cells from 3 and 6 of 10 healthy volunteers, respectively. Salmonella-specific IgA and/or IgG antibody responses in serum were observed for five (56%) of nine patients who had undergone colectomies and in 15 (88%) of 17 healthy volunteers. In ileostomy fluids, significant anti-Salmonella IgA antibody titer increases were detected in six (67%) of nine patients who had undergone colectomies. The impaired T- and B-cell immune responses found after vaccination in the circulation of patients who have undergone colectomies may be explained by a diminished colonization of the Ty21a vaccine strain due to the lack of a terminal ileum and colon.

scholarly journals Flagellin from Recombinant Attenuated Salmonella enterica Serovar Typhimurium Reveals a Fundamental Role in Chicken Innate Immunity

2012 ◽  
Vol 19 (3) ◽  
pp. 304-312 ◽  
Author(s):  
Zhiming Pan ◽  
Qiuxia Cong ◽  
Shizhong Geng ◽  
Qiang Fang ◽  
Xilong Kang ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Immune Responses ◽  
Salmonella Enterica ◽  
Rational Design ◽  
Bacterial Load ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Content Type ◽  
Serovar Typhimurium

ABSTRACTRecombinant attenuatedSalmonellavaccines have been extensively studied, with a focus on eliciting specific immune responses against foreign antigens. However, very little is known about the innate immune responses, particularly the role of flagellin, in the induction of innate immunity triggered by recombinant attenuatedSalmonellain chickens. In the present report, we describe twoSalmonella entericaserovar Typhimurium vaccine strains, wild-type (WT) or flagellin-deficient (flhD)Salmonella, both expressing the fusion protein (F) gene of Newcastle disease virus. We examined the bacterial load and spatiotemporal kinetics of expression of inflammatory cytokine, chemokine, and Toll-like receptor 5 (TLR5) genes in the cecum, spleen, liver, and heterophils following oral immunization of chickens with the twoSalmonellastrains. TheflhDmutant exhibited an enhanced ability to establish systemic infection compared to the WT. In contrast, the WT strain induced higher levels of interleukin-1β (IL-1β), CXCLi2, and TLR5 mRNAs in cecum, the spleen, and the heterophils than theflhDmutant at different times postinfection. Collectively, the present data reveal a fundamental role of flagellin in the innate immune responses induced by recombinant attenuatedSalmonellavaccines in chickens that should be considered for the rational design of novel vaccines for poultry.

scholarly journals Live Oral Typhoid Vaccine Ty21a Induces Cross-Reactive Humoral Immune Responses against Salmonella enterica Serovar Paratyphi A andS. Paratyphi B in Humans

2012 ◽  
Vol 19 (6) ◽  
pp. 825-834 ◽  
Author(s):  
Rezwanul Wahid ◽  
Raphael Simon ◽  
Shah J. Zafar ◽  
Myron M. Levine ◽  
Marcelo B. Sztein
Keyword(s):  
Immune Responses ◽  
Salmonella Enterica ◽  
Outer Membrane Proteins ◽  
Serum Antibody ◽  
Significant Proportion ◽  
Field Trials ◽  
Content Type ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Paratyphi B

ABSTRACTEnteric fever caused bySalmonella entericaserovar Paratyphi A infection has emerged as an important public health problem. Recognizing that in randomized controlled field trials oral immunization with attenuatedS. entericaserovar Typhi live vaccine Ty21a conferred significant cross-protection againstS. Paratyphi B but notS. Paratyphi A disease, we undertook a clinical study to ascertain whether humoral immune responses could explain the field trial results. Ty21a immunization of adult residents of Maryland elicited predominantly IgA antibody-secreting cells (ASC) that recognizeS. Typhi lipopolysaccharide (LPS). Cross-reactivity toS. Paratyphi A LPS was significantly lower than that toS. Paratyphi B LPS. ASC producing IgG and IgA that bind LPS from each of theseSalmonellaserovars expressed CD27 and integrin α4β7 (gut homing), with a significant proportion coexpressing CD62L (secondary lymphoid tissue homing). No significant differences were observed in serum antibody against LPS of the different serovars. Levels of IgA B memory (BM) cells toS. Typhi LPS were significantly higher than those againstS. Paratyphi A or B LPS, with no differences observed betweenS. Paratyphi A and B. The response of IgA BMto outer membrane proteins (OMP) fromS. Typhi was significantly stronger than that to OMP ofS. Paratyphi A but similar to that to OMP ofS. Paratyphi B. The percentages of IgG or IgA BMresponders to LPS or OMP from theseSalmonellastrains were similar. Whereas cross-reactive humoral immune responses toS. Paratyphi A or B antigens are demonstrable following Ty21a immunization, they cannot explain the efficacy data gleaned from controlled field trials.

scholarly journals Relationship between STAT5B Candidate Gene Polymorphism with Growth Related Traits and Ascites Index in Commercial Chicken Line

2018 ◽  
Vol 9 (20) ◽  
pp. 100-109 ◽  
Author(s):  
Elham Telphoni ◽  
Sadegh Alijani ◽  
Karim Hasanpour ◽  
Arash Javanmard ◽  
◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Candidate Gene ◽  
Chicken Line ◽  
Commercial Chicken

scholarly journals Candidate Live, Attenuated Salmonella enterica Serotype Typhimurium Vaccines with Reduced Fecal Shedding Are Immunogenic and Effective Oral Vaccines

2007 ◽  
Vol 75 (4) ◽  
pp. 1835-1842 ◽  
Author(s):  
Moataz Abd El Ghany ◽  
Angela Jansen ◽  
Simon Clare ◽  
Lindsay Hall ◽  
Derek Pickard ◽  
...  
Keyword(s):  
Immune Responses ◽  
Salmonella Enterica ◽  
Virulent Strain ◽  
Oral Vaccine ◽  
Oral Vaccines ◽  
Fecal Shedding ◽  
Live Vaccines ◽  
Cellular Immune Responses ◽  
Genetically Manipulated ◽  
Cellular Immune

ABSTRACT Environmental shedding of genetically manipulated microorganisms is an issue impeding the development of new live vaccines. We have investigated the immunogenicity of a number of novel Salmonella enterica serotype Typhimurium oral vaccine candidates that express the fragment C (TetC) component of tetanus toxin and harbor combinations of additional mutations in genes shdA, misL, and ratB that contribute to the persistence of serotype Typhimurium's colonization of the intestine. Serotype Typhimurium aroA (TetC) derivatives harboring additional mutations in either shdA or misL or combinations of these mutations exhibited a marked decrease in shedding of the vaccine strain in the feces of orally vaccinated mice. However, equivalent levels of anti-TetC and anti-Salmonella lipopolysaccharide immunoglobulin G (IgG), IgG1, IgG2a, and IgA were detected in sera of the vaccinated but not of the control mice. Cellular immune responses to TetC were detected in all vaccinated mice, regardless of the presence of the additional mutations in shdA or misL. Further, immunization with serotype Typhimurium aroA candidate vaccines harboring shdA and misL afforded complete protection against challenge with a virulent strain of serotype Typhimurium.

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