DETERMINATION OF THE NATURE OF THE CONFORMATIONAL TRANSMISSION EFFECT IN PENTACOORDINATED PHOSPHORUS COMPOUNDS

Determination of the phosphorus partition in blood is described, combining dry combustion with the ceruleo-molybdate procedure of Deniges. Advantages in speed and convenience are claimed over the conventional Briggs methods.

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Automated Analysis of Organophosphorus Insecticides by Wet Digestion-Oxidation and Colorimetric Determination of the Derived Orthophosphate

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/51.3.697 ◽

1968 ◽

Vol 51 (3) ◽

pp. 697-708

Author(s):

Daniel E Ott ◽

Francis A Gunther

Keyword(s):

Thin Layer ◽

Automated Analysis ◽

Automated System ◽

Phosphorus Compounds ◽

Colorimetric Determination ◽

Total System ◽

Blue Color ◽

Automated Technique ◽

Analytical System

Abstract Adaptation of an AutoAnalyzer system to the determination of phosphorus in organic compounds now provides an automated technique generally applicable for the analysis of organophosphorus inserticides. Following automated wet digestionoxidation of the compounds (some need preliminary alkaline hydrolysis) the resulting orthophosphate is determined automatically by a colorimetric system reading a phosphomolybdenum blue color at 815 mμ. The total system handles 10 samples per hour with a precision and sensitivity readily practical for organophosphorus insecticide analysis; the insecticidal solutions tested, at concentrations of 2 μg/ml or greater, gave repeatable responses within ± 10%. Manual cleanup procedures for most crop samples are required to remove interfering noninsecticidal phosphorus compounds before the system is applicable to total residue analyses. Thimet (phorate) and phosphamidon at 0.5 ppm in fortified stripping solutions of strawberries were analyzed separately without cleanup, however. Adsorption column chromatography on Florisil has provided rapid and efficient cleanup of fortified spinach extractives prior to automated analysis of Thimet and dimethoate. Autoanalysis of individual spots from thin layer chromatograms provides considerable specificity to this nonspecific analytical system, and for some crops provides all the cleanup necessary. Thin layer "spots" can be introduced directly into the automated system.

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Gas-Liquid Chromatographic Determination of Dioxathion and Quintiofos in Organophosphorus Dip Washes

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/63.1.33 ◽

1980 ◽

Vol 63 (1) ◽

pp. 33-36

Author(s):

Molly I Keating

Keyword(s):

Solvent Extraction ◽

Extraction Procedure ◽

Internal Standard ◽

Chromatographic Determination ◽

Phosphorus Compounds ◽

Ionization Detector ◽

Liquid Chromatographic Determination ◽

Gasliquid Chromatography ◽

Liquid Chromatographic

Abstract A rapid method for the analysis of dip washes is described which eliminates the usual solvent extraction procedure. The dip wash is initially diluted with acetone and then with petroleum ether. The diluted dip wash is analyzed by gasliquid chromatography, using an alkali ionization detector sensitive to phosphorus compounds. The method was applied to the determination of dioxathion (2,3-ρ-dioxanedithiol S,S-bis(O,O-diethyl phosphorodithioate)), and quintiofos (O-ethyl O-8-quinolyl phenylphosphonothioate) dip washes. Average recoveries from fouled dip washes were 100 and 104%. GLC of these compounds with an internal standard is described, which improves the precision of the method to ±2%.

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Behaviour of Intracellular Polyphosphate in the Biological Phosphate Removal Process

Water Science & Technology ◽

10.2166/wst.1985.0218 ◽

1985 ◽

Vol 17 (11-12) ◽

pp. 11-21 ◽

Cited By ~ 19

Author(s):

Takashi Mino ◽

Tomonori Kawakami ◽

Tomonori Matsuo

Keyword(s):

Phosphorus Removal ◽

Point Of View ◽

Phosphate Removal ◽

Phosphorus Compounds ◽

Biological Phosphorus Removal ◽

Removal Process ◽

Phosphorus Source ◽

Biological Phosphorus ◽

Intracellular Phosphorus

In this study the functions of the intracellular polyphosphate in the biological phosphorus removal process were investigated from biological point of view. The STS method was used for the determination of polyphosphate and the fractionation of the intracellular phosphorus compounds. The lowmolecular polyphosphate and the highmolecular polyphosphate can be determined separately in this method. The radiotracer experiments were performed in which 32P-labeled orthophosphate was used as a tracer. The chemical analyses of phosphorus and the radioactivity measurement were made simultaniously in the course of the anaerobic oxic process. From the results of a radiotracer experiment the mass transfer of phosphorus among the phosphorus compounds was calculated. It was suggested that the lowmolecular polyphosphate served as the energy source under the anaerobic conditions and that the highmolecular polyphosphate served as the phosphorus source for the growth reactions. Some models for the phosphorus transfer in the high phosphorus accumulating microorganisms were proposed.

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Problems Involved in the Quantitative Extraction of Glycogen and High-Energy Phosphorus Compounds in Frozen Cod Muscle

Journal of the Fisheries Research Board of Canada ◽

10.1139/f68-138 ◽

1968 ◽

Vol 25 (8) ◽

pp. 1525-1538 ◽

Cited By ~ 3

Author(s):

Sandra S. Nowlan ◽

W. J. Dyer ◽

Doris I. Fraser

Keyword(s):

Liquid Nitrogen ◽

Perchloric Acid ◽

Potassium Hydroxide ◽

Sampling Error ◽

High Energy ◽

Phosphorus Compounds ◽

Quantitative Extraction ◽

Sampling Procedures ◽

Acid Extractant

Extraction of cod muscle with 0.3 N perchloric acid followed by digestion of the residue in potassium hydroxide yielded an average of 16% more total glycogen than did classical digestion with 30% KOH. However, the differences tended to be less at higher glycogen levels. It was suggested that glycogen may be partially degraded during digestion with KOH, although no glycogenosis occurred during contact with alkali prior to heating. The proportion of residual glycogen not extracted by acid varied from 23% in unfrozen muscle to about 40% in liquid nitrogen-frozen and in slowly frozen muscle.Significant degradation of glycogen and high energy phosphorus compounds in frozen prerigor cod muscle was avoided by weighing samples in insulated beakers chilled by liquid nitrogen to prevent warming or thawing, and by homogenizing immediately on addition of acid extractant. Extraction with acid enabled the simultaneous determination of glycogen and phosphorus compounds on the same sample. Special sampling procedures were employed to reduce the sampling error due to variation in glycogen distribution along the fillet.

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The Impact of the Quality of Tap Water and the Properties of Installation Materials on the Formation of Biofilms

Water ◽

10.3390/w11091903 ◽

2019 ◽

Vol 11 (9) ◽

pp. 1903 ◽

Cited By ~ 3

Author(s):

Dorota Papciak ◽

Barbara Tchórzewska-Cieślak ◽

Andżelika Domoń ◽

Anna Wojtuś ◽

Jakub Żywiec ◽

...

Keyword(s):

Tap Water ◽

Galvanized Steel ◽

Phosphorus Compounds ◽

Plate Count ◽

Nickel Steel ◽

Quantitative Analyses ◽

The Impact ◽

First Time

The article presents changes in the quality of tap water depending on time spent in installation and its impact on the creation of biofilms on various materials (polyethylene (PE), polyvinyl chloride (PVC), chrome-nickel steel and galvanized steel). For the first time, quantitative analyses of biofilm were performed using methods such as: Adenosine 5’-triphosphate (ATP) measurement, flow cytometry, heterotrophic plate count and using fractographical parameters. In the water, after leaving the experimental installation, the increase of turbidity, content of organic compounds, nitrites and nitrates was found, as well as the decrease in the content of chlorine compounds, dissolved oxygen and phosphorus compounds. There was an increase in the number of mesophilic and psychrophilic bacteria. In addition, the presence of Escherichia coli was also found. The analysis of the quantitative determination of microorganisms in a biofilm indicates that galvanized steel is the most susceptible material for the adhesion of microorganisms. These results were also confirmed by the analysis of the biofilm morphology. The roughness profile, the thickness of the biofilm layer can be estimated at about 300 μm on galvanized steel.

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