Potato spindle tuber viroid is not encapsidated in vivo by potato virus Y particles

1992 ◽  
Vol 14 (1) ◽  
pp. 18-21 ◽  
Author(s):  
R.P. Singh ◽  
A. Boucher ◽  
R.G. Wang ◽  
T.H. Somerville
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Spindle Tuber Viroid

scholarly journals Fluorescently Tagged Potato virus Y: A Versatile Tool for Functional Analysis of Plant-Virus Interactions

2015 ◽  
Vol 28 (7) ◽  
pp. 739-750 ◽  
Author(s):  
Matevz Rupar ◽  
Florence Faurez ◽  
Michel Tribodet ◽  
Ion Gutiérrez-Aguirre ◽  
Agnès Delaunay ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Potato Virus ◽  
Plant Virus ◽  
Potato Virus Y ◽  
Fluorescent Protein ◽  
Ex Vivo ◽  
Cell Movement ◽  
Long Distance ◽  
Green Fluorescent

Potato virus Y (PVY) is an economically important plant virus that infects Solanaceous crops such as tobacco and potato. To date, studies into the localization and movement of PVY in plants have been limited to detection of viral RNA or proteins ex vivo. Here, a PVY N605 isolate was tagged with green fluorescent protein (GFP), characterized and used for in vivo tracking. In Nicotiana tabacum cv. Xanthi, PVY N605-GFP was biologically comparable to nontagged PVY N605, stable through three plant-to-plant passages and persisted for four months in infected plants. GFP was detected before symptoms and fluorescence intensity correlated with PVY RNA concentrations. PVY N605-GFP provided in vivo tracking of long-distance movement, allowing estimation of the cell-to-cell movement rate of PVY in N. tabacum cv. Xanthi (7.1 ± 1.5 cells per hour). PVY N605-GFP was adequately stable in Solanum tuberosum cvs. Désirée and NahG-Désirée and able to infect S. tuberosum cvs. Bintje and Bea, Nicotiana benthamiana, and wild potato relatives. PVY N605-GFP is therefore a powerful tool for future studies of PVY-host interactions, such as functional analysis of viral and plant genes involved in viral movement.

scholarly journals Use of a vector based on Potato virus X in a whole plant assay to demonstrate nuclear targeting of Potato spindle tuber viroid

2001 ◽  
Vol 82 (6) ◽  
pp. 1491-1497 ◽  
Author(s):  
Yan Zhao ◽  
Robert A. Owens ◽  
Rosemarie W. Hammond
Keyword(s):  
Potato Virus ◽  
Rna Splicing ◽  
Fluorescent Protein ◽  
Potato Virus X ◽  
Potato Spindle Tuber Viroid ◽  
Host Cells ◽  
Coding Region ◽  
Nuclear Targeting ◽  
Whole Plant

Potato spindle tuber viroid (PSTVd) is a covalently closed circular RNA molecule of 359 nucleotides that replicates within the nucleus of host cells. To determine how this small, highly structured RNA enters the nucleus, we have developed a virus-based, whole plant in vivo assay that uses green fluorescent protein (GFP) as the reporter molecule. The coding region of GFP was interrupted by insertion of an intron derived from the intervening sequence 2 of the potato ST-LS1 gene. A cDNA copy of the complete PSTVd genome was, in turn, embedded within the intron, and this construct was delivered into Nicotiana benthamiana plants via a vector based on Potato virus X. The intron-containing GFP subgenomic RNA synthesized during virus infection cannot produce a functional GFP unless the RNA is imported into the nucleus, where the intron can be removed and the spliced RNA returned to the cytoplasm. The appearance of green fluorescence in leaf tissues inoculated with constructs containing a full-length PSTVd molecule embedded in the intron indicates that nuclear import and RNA splicing events did occur.

scholarly journals Survival and Transmission of Potato Virus Y, Pepino Mosaic Virus, and Potato Spindle Tuber Viroid in Water

2013 ◽  
Vol 80 (4) ◽  
pp. 1455-1462 ◽  
Author(s):  
N. Mehle ◽  
I. Gutiérrez-Aguirre ◽  
N. Prezelj ◽  
D. Delić ◽  
U. Vidic ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Potato Virus ◽  
Plant Pathogens ◽  
Potato Virus Y ◽  
Potato Spindle Tuber Viroid ◽  
Plant Viruses ◽  
Detection Methods ◽  
Mechanical Transmission ◽  
Pepino Mosaic Virus ◽  
Viroid Infection

ABSTRACTHydroponic systems and intensive irrigation are used widely in horticulture and thus have the potential for rapid spread of water-transmissible plant pathogens. Numerous plant viruses have been reported to occur in aqueous environments, although information on their survival and transmission is minimal, due mainly to the lack of effective detection methods and to the complexity of the required transmission experiments. We have assessed the role of water as a source of plant infection using three mechanically transmissible plant pathogens that constitute a serious threat to tomato and potato production: pepino mosaic virus (PepMV), potato virus Y (PVY), and potato spindle tuber viroid (PSTVd). PepMV remains infectious in water at 20 ± 4°C for up to 3 weeks, PVY (NTN strain) for up to 1 week, and PSTVd for up to 7 weeks. Experiments using a hydroponic system show that PepMV (Ch2 genotype) and PVY (NTN strain) can be released from plant roots into the nutrient solution and can infect healthy plants through their roots, ultimately spreading to the green parts, where they can be detected after a few months. In addition, tubers developed on plants grown in substrate watered with PSTVd-infested water were confirmed to be the source of viroid infection. Our data indicate that although well-known pathways of virus spread are more rapid than water-mediated infection, like insect or mechanical transmission through leaves, water is a route that provides a significant bridge for rapid virus/viroid spread. Consequently, water should be taken into account in future epidemiology and risk assessment studies.

scholarly journals Genetic Diversity of Potato virus Y in Potato Production Areas in Northeast China

Plant Disease ◽  
2019 ◽  
Vol 103 (2) ◽  
pp. 289-297 ◽  
Author(s):  
Yanju Bai ◽  
Shuxin Han ◽  
Yanling Gao ◽  
Wei Zhang ◽  
Guoquan Fan ◽  
...  
Keyword(s):  
Potato Virus ◽  
Inner Mongolia ◽  
Potato Virus Y ◽  
Potato Cultivar ◽  
Potato Production ◽  
Potato Spindle Tuber Viroid ◽  
Single Strain ◽  
Strain Group ◽  
Common Potato ◽  
Genome Level

In 2011–2014, ELISA or nucleic acid spot hybridization (NASH) testing for common potato viruses or Potato spindle tuber viroid (PSTVd) was performed on 500 leaf samples collected in potato fields in the northeast provinces Heilongjiang and Inner Mongolia, China. The results revealed that 38.4% (Heilongjiang) and 27.7% (Inner Mongolia) were positive for Potato virus Y (PVY). To unveil the strain composition and population structure of PVY in the region, the multiplex RT-PCR described by Chikh-Ali et al. was performed on all of the ELISA-PVY-positive samples. Of the 158 samples whose PVY strain scenarios could be determined, PVYNTN-NW-SYR-II and PVYN-Wi were the most abundant strains, occurring in 58.9 and 47.5% samples, followed by PVYNTN-NW-SYR-I (31.0%), PVYN:O (19.6%), Eu-PVYNTN (7.6%), NA-PVYN (1.3%), and PVYO (0.6%). In the 84 single-strain-infected samples, PVYN-Wi accounted for 41.7%, PVYNTN-NW-SYR-II for 40.5%, PVYNTN-NW-SYR-I for 14.3%, and PVYN:O and Eu-PVYNTN for 3.6% each. Seven isolates representing PVYNTN-NW-SYR-I (HLJ-6-1 and HLJ-9-4), PVYNTN-NW-SYR-II (INM-W-369-12 and SC-1-1-2), PVYN:O (HLJ-30-2), and PVYN-Wi (HLJ-BDH-2 and HLJ-C-429) were sequenced and analyzed molecularly. Whereas the sequence identities for isolates belonging to the same strain group were >98.5%, they fell for isolates belonging to different strain groups to 92.7–98.1% at the genome level and 96.1–98.4% at the polyprotein level. Interestingly, the exact location of the recombination events varied among isolates within a strain group. Phylogenetic analysis of all 42 full length PVY sequences from China indicated that most clustered to various recombinant groups, despite the fact that the PVY isolates were isolated from at least five host species. Pathological analysis of four isolates representing PVYN:O, PVYN-Wi, PVYNTN-NW-SYR-I, and PVYNTN-NW-SYR-II revealed that the PVYNTN-NW-SYR-II isolate incited the most severe symptoms on potato cultivar Kexin 13, followed by PVYNTN-NW-SYR-I, PVYN:O and PVYN-Wi. The PVYNTN-NW-SYR-I and PVYNTN-NW-SYR-II isolates also caused necrotic ringspots on the tubers of Kexin 13, indicating their ability to induce the potato tuber necrotic ringspot disease in potato.

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