Obesity, dyslipidaemia and candidate gene polymorphisms: a cross-sectional study among the Liangmai and Mizo tribes of Manipur, India

ObjectivesRecently, genome-wide associated studies have identified several genetic loci that are associated with elevated blood pressure and could play a critical role in intracellular calcium homeostasis. The aim of this study was to assess the associations ofATP2B1rs2681472 andCACNB2rs12258967 gene polymorphisms with high blood pressure (HBP) among Lithuanian children and adolescents aged 12–15 years.Study design and participantsThis was a cross-sectional study of a randomly selected sample of 646 12–15-year-old adolescents who participated in the survey ‘The Prevalence and Risk Factors of HBP in 12–15 Year-Old Lithuanian Children and Adolescents (from November 2010 to April 2012)’. Anthropometric parameters and BP were measured. The participants with HBP were screened on two separate occasions. Subjects were genotypedATP2B1rs2681472 andCACNB2rs12258967 gene polymorphisms using real-time PCR method.ResultsThe prevalence of HBP was 36.7%, significantly higher for boys than for girls. In the multivariate analysis, after adjustment for body mass index and waist circumference, boys withCACNB2CG genotype,CACNB2GG genotype andCACNB2CG +GG genotype had higher odds of having HBP in codominant (adjusted OR (aOR)=1.92; 95% CI 1.16 to 3.18, p=0.011; and aOR=2.64; 95% CI 1.19 to 5.90, p=0.018) and in dominant (aOR=2.05; 95% CI 1.27 to 3.30, p=0.003) inheritance models. Girls carryingCACNB2CG genotype andCACNB2CG +GG genotype had increased odds of HBP in codominant (aOR=1.82; 95% CI 1.02 to 3.24, p=0.044) and in dominant (aOR=1.89; 95% CI 1.09 to 3.28, p=0.023) inheritance models. Furthermore, significant associations were found in additive models separately for boys (aOR=1.72; 95% CI 1.20 to 2.46, p=0.003) and girls (aOR=1.52; 95% CI 1.05 to 2.20, p=0.027). No significant association was found betweenATP2B1gene polymorphism and the odds of HBP.ConclusionsOur results indicate thatCACNB2gene polymorphism was significantly associated with higher odds of HBP in Lithuanian adolescents aged 12–15 years.

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Comparison of endocannabinoids levels, FAAH gene polymorphisms, and appetite regulatory substances in women with and without binge eating disorder: a cross- sectional study

Nutrition Research ◽

10.1016/j.nutres.2020.09.001 ◽

2020 ◽

Vol 83 ◽

pp. 86-93

Author(s):

Neda Lotfi Yagin ◽

Fereshteh Aliasgari ◽

Mohammad Alizadeh ◽

Soghra Aliasgharzadeh ◽

Reza Mahdavi

Keyword(s):

Eating Disorder ◽

Binge Eating ◽

Binge Eating Disorder ◽

Gene Polymorphisms ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional

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CYP1A2 Gene Polymorphism and Theophylline Level in Asthma

The Indonesian Biomedical Journal ◽

10.18585/inabj.v11i1.475 ◽

2019 ◽

Vol 11 (1) ◽

pp. 63-9

Author(s):

Amelia Lorensia ◽

Zullies Ikawati ◽

Tri Murti Andayani ◽

Daniel Maranatha ◽

Mariana Wahyudi

Keyword(s):

Gene Polymorphism ◽

Gene Polymorphisms ◽

Asthma Exacerbation ◽

Cross Sectional Study ◽

Sectional Study ◽

Chi Square ◽

Cross Sectional ◽

Theophylline Level ◽

Significant Difference ◽

Polymorphism Data

BACKGROUND: Aminophylline (theophylline) is one of the most frequent asthma therapies in Indonesia, although it remains as a narrow therapy. The effects of drugs are individualized and strongly influenced by genetic, one of which is CYP1A2 gene polymorphisms. This study aimed to determine the profile of CYP1A2 polymorphism and theophylline level in asthma exacerbation patients receiving intravenous aminophylline therapy.METHODS: This cross sectional study was conducted in the emergency room (ER), to adults asthma exacerbation patients without complication (n=27), visiting the ER. The gene polymorphism data were compared with theophylline levels in the blood using chi-square test.RESULTS: In the CYP1A2 gene polymorphism profile, the most common heterozygous alleles are T/G genotype of CYP1A2*1E and C/A genotype of CYP1A2*1F. Most homozygote alleles exist in CYP1A2*1D and CYP1A2*1F. There was significant difference between CYP1A2*1D (p<0.005), CYP1A2*1E (p<0.023) and CYP1A2*1F (p<0.000) polymorphisms and theophylline level.CONCLUSION: CYP1A2*1D, CYP1A2*1E and CYP1A2*1F gene polymorphisms had an effect on theophylline levels. However, no one experienced an overdose theophylline, and no correlation between theophylline levels with CYP1A2 gene polymorphism.KEYWORDS: exacerbation asthma, intravenous aminophylline, CYP1A2 polymorphism gene, theophylline

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ASSOCIATION OF CORTICOTROPHIN RELEASING HORMONE RECEPTOR 1 GENE POLYMORPHISMS (RS242941 AND RS242939) WITH PERSISTENT ASTHMA AND ITS PHENOTYPE IN NORTHERN INDIAN ASTHMATIC CHILDREN: A CROSS‑SECTIONAL STUDY

Indian Journal of Medical Sciences ◽

10.18203/issn.0019-5359.indianjmedsci20163530 ◽

2016 ◽

Vol 68 (1) ◽

pp. 42

Author(s):

Sarika Gupta ◽

Shally Awasthi ◽

Neeraj Sharma ◽

Sarita Agarwal ◽

Priya Tripathi

Keyword(s):

Hormone Receptor ◽

Gene Polymorphisms ◽

Persistent Asthma ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional ◽

Releasing Hormone ◽

Asthmatic Children ◽

Potential Marker ◽

Increased Risk

INTRODUCTION: Asthma is a common, chronic, relapsing disease in children. Corticotrophin‑releasing hormone receptor 1 (CRHR1) gene is identified as a potential marker for steroid responsiveness. Genetic variations in CRHR1 are expected to diminish the capacity to secrete endogenous cortisol. OBJECTIVE: This study aimed to find out association of CRHR1 gene polymorphisms namely, rs242941 (G > T) and rs242939 (A > G) with persistent asthma and its phenotype in Northern Indian asthmatic children. MATERIALS AND METHODS: This was a hospital‑based cross‑sectional study. Genotyping was done for 250 asthmatic children, aged 1‑12 years, using polymerase chain reaction restriction fragment length polymorphisms method. RESULTS: Mutant homozygous genotype (TT) and mutant allele (T) of single nucleotide polymorphism (SNP) rs242941 were found to be associated with increased risk for persistent asthma (odds ratio [OR] =4.2; 95% confidence interval [CI] =1.6‑10.9; P = 0.00 and OR = 1.8; 95% CI = 1.2‑2.8; P = 0.00, respectively). On analyzing genotypic and phenotypic associations, factors such as urban residence (OR = 2.01; 95% CI = 1.11‑3.63; P = 0.01), family history of asthma (OR = 1.80; 95% CI = 1.00‑3.24; P = 0.05), previous hospitalization (OR = 2.12; 95% CI = 1.14‑3.96; P = 0.01), previous use of bronchodilators (OR = 3.64; 95% CI = 1.68‑7.94; P = 0.00), previous use of inhaled corticosteroids (OR = 2.37; 95% CI = 1.15‑4.93; P = 0.01), visit to doctor in last 1 year (OR = 1.82; 95% CI = 1.01‑3.28; P = 0.04), seasonal variation in exacerbation (OR = 2.66; 95% CI = 1.16‑6.12; P = 0.01), and lower pulmonary functions ( P = 0.00) were found to be associated with SNP rs242941. Genotypes of SNP rs242939 showed no association with persistent asthma and its phenotype. Minor allele frequency for SNP rs242941 and SNP rs242939 was 43.20% and 11.00%, respectively, in Northern Indian asthmatic children. CONCLUSION: In conclusion, we observed an association of SNP rs242941 with persistent asthma and its phenotype in Northern Indian asthmatic children.

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