Impediment to growth and yeast-to-hyphae transition in Candida albicans by copper oxide nanoparticles

Biofouling ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 56-72 ◽  
Author(s):  
Alwar Ramanujam Padmavathi ◽  
Sriyutha Murthy P. ◽  
Arindam Das ◽  
Arumugam Priya ◽  
T. J. Sushmitha ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Biosynthesis of Copper Oxide Nanoparticles Using Streptomyces MHM38 and Its Biological Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Sarah I. Bukhari ◽  
Moaz M. Hamed ◽  
Mohamed H. Al-Agamy ◽  
Hanaa S. S. Gazwi ◽  
Hesham H. Radwan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Copper Oxide ◽  
Salmonella Typhimurium ◽  
Histopathological Examination ◽  
Copper Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Enzymatic Antioxidants ◽  
The Impact

Biosynthesis methods employing microorganisms have emerged as an eco-friendly, clean, and viable alternative to chemical and physical processes. The present study reports the synthesis of copper oxide nanoparticles (CuONPs) using cell-free culture supernatant of marine Streptomyces sp. MHM38. For the optimized production of CuONPs, the influence of some parameters, such as the concentration of copper sulfate (CuSO4), reaction time, filtrate to substrate ratio, and pH, was studied. 5 mM of CuSO4 was optimal for nanoparticle (NP) production. Well-defined CuONP formation occurred after 60 min of incubation when an equal volume of filtrate (cell-free supernatant) to substrate (CuSO4 solution) was added. UV-visible spectroscopy analysis of CuONPs exhibited a peak at 550 nm, which corresponds to the surface plasmon resonance of CuONPs. Most of the particles were spherical and were 1.72–13.49 nm when measured using a transmission electron microscope. The antimicrobial activity of CuONPs was determined using a well diffusion method against Enterococcus faecalis ATCC 29212, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8939, fungi (Rhizoctonia solani, Fusarium solani, and Aspergillus niger), and yeast (Candida albicans ATCC 10237). The highest antimicrobial activities were recorded against Candida albicans ATCC 10237, whereas Salmonella typhimurium ATCC 14028 and Escherichia coli ATCC 8939 showed the less activity. The biochemical findings of the CuONP groups were significant ( p < 0.05 ) with diminished levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total and direct bilirubin, urea, and creatinine compared with the paracetamol group. Nonenzymatic and enzymatic antioxidants of the CuONP groups were significantly elevated ( p < 0.05 ) in SOD and GSH levels, and exceptionally low nitric oxide (NO) and malondialdehyde (MAD) levels were found for the paracetamol group. The histopathological examination of the CuONP groups assured the impact of improving CuONPs against paracetamol-induced liver damage.

scholarly journals Biosynthesis of Copper Oxide Nanoparticles by Marine Streptomyces MHM38 and their Preventive Efficacy Against Paracetamol-Inducing Hepatic Damage of Albino Rats

2020 ◽  
Author(s):  
Moaz Hamed ◽  
Hanaa S.S. Gazwi ◽  
Asmaa M. Youssif
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Copper Oxide ◽  
Salmonella Typhimurium ◽  
Copper Oxide Nanoparticles ◽  
Diffusion Method ◽  
Albino Rats ◽  
Oxide Nanoparticles ◽  
Vis Spectroscopy ◽  
Preventive Efficacy

Abstract Biosynthesis methods, employing microorganisms, have emerged as an eco-friendly, clean and viable alternative to chemical and physical methods. The present study reports the biosynthesis of copper oxide nanoparticles (CuONPs) using cell-free culture supernatant of marine Streptomyces sp. MHM38. For the optimized production of copper oxide nanoparticles, the influence of some parameters such as concentration of copper sulphate, reaction time, filtrate to substrate ratio and pH were studied. 5mM CuSO4 was optimal for NP production. Well-defined CuONP formation occurred after 60 min incubation when equal volume of filtrate (cell-free supernatant) to substrate (CuSO4 solution) was added. NPs remained stable in aqueous solution with increasing time at pH 8. CuONPs were characterized by UV-vis spectroscopy, X-ray diffraction (XRD) and finally the nature of the nanoparticles was identified by elemental analysis (EDX). Uv-vis spectroscopy of CuONPs exhibited peak at 550 nm which corresponds to the Surface Plasmon Resonance of CuONPs. Most of the particles are spherical in shape and size ranges from 1.06 – 6.5 nm analyzed using Transmission Electron Microscope (TEM). Antimicrobial activity of CuONPs was performed by well diffusion method against Enterococcus faecalis ATCC 29212, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8939), fungi (Rhizoctonia solani, Fusarium solani, Aspergillus niger) and yeast (Candida albicans ATCC 10237) .The highest antimicrobial activities recorded were against Candida albicans ATCC 10237, were as Salmonella typhimurium ATCC 14028 and Escherichia coli ATCC 8939 showed the lesser activity. The preventive efficacy of CuONPs was evaluated against the oxidative stress induced by paracetamol (PAC) in albino rats. The biochemical findings of CuONPs groups appeared a significant (p˂0.05) diminish in the levels of ALT, AST, ALP, LDH, total and direct bilirubin, Urea, and Creatinine as compared to paracetamol group. Non-enzymatic and enzymatic antioxidants of CuONPs groups were significantly elevated (p˂0.05) in SOD and GSH levels and significantly low NO and MAD levels compared to the paracetamol group. Also, the histopathological examination of the CuONPs groups assured that the impact of improving CuONPs against paracetamol-induced liver damage.

ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Pseudomonas Fluorescens ◽  
Wide Spectrum ◽  
Copper Oxide Nanoparticles ◽  
Morphological Properties ◽  
Clinical Samples ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

Green synthesis of copper oxide nanoparticles using aloe vera extract

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Hemalatha D ◽  
Saraswath S
Keyword(s):  
Copper Oxide ◽  
Biomedical Application ◽  
Aloe Vera ◽  
Copper Oxide Nanoparticles ◽  
Klebsiella Pneumonia ◽  
Oxide Nanoparticles ◽  
Cupric Sulfate ◽  
X Ray Diffraction ◽  
Fouriertransform Infrared Spectroscopy ◽  
Effective Use

In material science, green method for synthesis of nanomaterials is feasible, cheaper and eco-friendly protocol. To accomplish this phenomenon, present study was aimed to synthesize Copper oxide nanoparticles using leaf extract of Aloevera with two different precursors CuCl2.2H2O (Cupric chloride) and CuSo4.5H2O (Cupric sulfate). The extraction of Aloevera is employed as reducing and stabilizing agent for this synthesis.Copper oxide Nanoparticles is effective use of biomedical application due to their antibacterial function. The synthesized Copper oxide nanoparticles were characterized by X-Ray Diffraction Spectroscopy (XRD), Energy Dispersive Spectroscopy (EDX), FourierTransform Infrared Spectroscopy (FT- IR) and Scanning Electron Microscope(SEM). XRD studies reveal the crystallographic nature of Copper oxide nanoparticles. Furthermore the Copper oxide nanoparticles have good Antibacterial activity against both gram negative (E.Coli, Klebsiella pneumonia) and gram positive (Bacillus cereus, Staphylococcus aureus)bacteria.

Green Synthesis of Copper Oxide Nanoparticles using Cucumis sativus (cucumber) Extracts and their Bio-physical and Biochemical Characterization for Cosmetic and Dermatologic Applications

2020 ◽  
Vol 20 ◽  
Author(s):  
Monika Vats ◽  
Shruti Bhardwaj ◽  
Arvind Chhabra
Keyword(s):  
Copper Oxide ◽  
Green Synthesis ◽  
Biochemical Characterization ◽  
Chemical Characterization ◽  
Ultra Violet ◽  
Skin Penetration ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Ultra Violet Radiation ◽  
Anti Fungal

Background & Objective: Nanoparticles are used in cosmetic and dermatologic products, due to better skin penetration properties. Incorporation of natural products exhibiting medicinal properties in nano-preparations could significantly improve efficacy of these products and improve the quality of life without the side effects of synthetic formulations. Methods: We here report green synthesis of Copper Oxide nanoparticles, using Cucumber extract, and their detailed biophysical and bio-chemical characterization. Results: These Copper Oxide-Cucumber nanoparticles exhibit significant anti-bacterial and anti-fungal properties, Ultra Violet-radiation protection ability and reactive-oxygen species inhibition properties. Importantly, these nanoparticles do not exhibit significant cellular toxicity and, when incorporated in skin cream, exhibit skin rejuvenating properties. Conclusion: Our findings have implications for nanoparticle-based cosmetics and dermatologic applications.

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