scholarly journals Biosynthesis of Copper Oxide Nanoparticles Using Streptomyces MHM38 and Its Biological Applications

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Sarah I. Bukhari ◽  
Moaz M. Hamed ◽  
Mohamed H. Al-Agamy ◽  
Hanaa S. S. Gazwi ◽  
Hesham H. Radwan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Copper Oxide ◽  
Salmonella Typhimurium ◽  
Histopathological Examination ◽  
Copper Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Enzymatic Antioxidants ◽  
The Impact

Biosynthesis methods employing microorganisms have emerged as an eco-friendly, clean, and viable alternative to chemical and physical processes. The present study reports the synthesis of copper oxide nanoparticles (CuONPs) using cell-free culture supernatant of marine Streptomyces sp. MHM38. For the optimized production of CuONPs, the influence of some parameters, such as the concentration of copper sulfate (CuSO4), reaction time, filtrate to substrate ratio, and pH, was studied. 5 mM of CuSO4 was optimal for nanoparticle (NP) production. Well-defined CuONP formation occurred after 60 min of incubation when an equal volume of filtrate (cell-free supernatant) to substrate (CuSO4 solution) was added. UV-visible spectroscopy analysis of CuONPs exhibited a peak at 550 nm, which corresponds to the surface plasmon resonance of CuONPs. Most of the particles were spherical and were 1.72–13.49 nm when measured using a transmission electron microscope. The antimicrobial activity of CuONPs was determined using a well diffusion method against Enterococcus faecalis ATCC 29212, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8939, fungi (Rhizoctonia solani, Fusarium solani, and Aspergillus niger), and yeast (Candida albicans ATCC 10237). The highest antimicrobial activities were recorded against Candida albicans ATCC 10237, whereas Salmonella typhimurium ATCC 14028 and Escherichia coli ATCC 8939 showed the less activity. The biochemical findings of the CuONP groups were significant ( p < 0.05 ) with diminished levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total and direct bilirubin, urea, and creatinine compared with the paracetamol group. Nonenzymatic and enzymatic antioxidants of the CuONP groups were significantly elevated ( p < 0.05 ) in SOD and GSH levels, and exceptionally low nitric oxide (NO) and malondialdehyde (MAD) levels were found for the paracetamol group. The histopathological examination of the CuONP groups assured the impact of improving CuONPs against paracetamol-induced liver damage.

scholarly journals Biosynthesis of Copper Oxide Nanoparticles by Marine Streptomyces MHM38 and their Preventive Efficacy Against Paracetamol-Inducing Hepatic Damage of Albino Rats

2020 ◽  
Author(s):  
Moaz Hamed ◽  
Hanaa S.S. Gazwi ◽  
Asmaa M. Youssif
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Copper Oxide ◽  
Salmonella Typhimurium ◽  
Copper Oxide Nanoparticles ◽  
Diffusion Method ◽  
Albino Rats ◽  
Oxide Nanoparticles ◽  
Vis Spectroscopy ◽  
Preventive Efficacy

Abstract Biosynthesis methods, employing microorganisms, have emerged as an eco-friendly, clean and viable alternative to chemical and physical methods. The present study reports the biosynthesis of copper oxide nanoparticles (CuONPs) using cell-free culture supernatant of marine Streptomyces sp. MHM38. For the optimized production of copper oxide nanoparticles, the influence of some parameters such as concentration of copper sulphate, reaction time, filtrate to substrate ratio and pH were studied. 5mM CuSO4 was optimal for NP production. Well-defined CuONP formation occurred after 60 min incubation when equal volume of filtrate (cell-free supernatant) to substrate (CuSO4 solution) was added. NPs remained stable in aqueous solution with increasing time at pH 8. CuONPs were characterized by UV-vis spectroscopy, X-ray diffraction (XRD) and finally the nature of the nanoparticles was identified by elemental analysis (EDX). Uv-vis spectroscopy of CuONPs exhibited peak at 550 nm which corresponds to the Surface Plasmon Resonance of CuONPs. Most of the particles are spherical in shape and size ranges from 1.06 – 6.5 nm analyzed using Transmission Electron Microscope (TEM). Antimicrobial activity of CuONPs was performed by well diffusion method against Enterococcus faecalis ATCC 29212, Salmonella typhimurium ATCC 14028, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8939), fungi (Rhizoctonia solani, Fusarium solani, Aspergillus niger) and yeast (Candida albicans ATCC 10237) .The highest antimicrobial activities recorded were against Candida albicans ATCC 10237, were as Salmonella typhimurium ATCC 14028 and Escherichia coli ATCC 8939 showed the lesser activity. The preventive efficacy of CuONPs was evaluated against the oxidative stress induced by paracetamol (PAC) in albino rats. The biochemical findings of CuONPs groups appeared a significant (p˂0.05) diminish in the levels of ALT, AST, ALP, LDH, total and direct bilirubin, Urea, and Creatinine as compared to paracetamol group. Non-enzymatic and enzymatic antioxidants of CuONPs groups were significantly elevated (p˂0.05) in SOD and GSH levels and significantly low NO and MAD levels compared to the paracetamol group. Also, the histopathological examination of the CuONPs groups assured that the impact of improving CuONPs against paracetamol-induced liver damage.

Development of Copper Loaded Nanoparticles Hydrogel Made from Waste Biomass (Sugarcane Bagasse) for Special Medical Application

2020 ◽  
Vol 847 ◽  
pp. 102-107
Author(s):  
Harish Kumar ◽  
Avneesh Kumar Gehlaut ◽  
Himanshu Gupta ◽  
Ankur Gaur ◽  
Jin Won Park
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Copper Oxide ◽  
Sugarcane Bagasse ◽  
Copper Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Waste Biomass ◽  
Cuo Nps ◽  
Vis Spectroscopy

This research is based on the creation of copper oxide nanoparticles (CuO-NPs) hydrogel through in situ method. The effective UV-Vis spectroscopy absorption peak of 610 nm indicates the existence of copper oxide nanoparticles (CuO-NP) group because of surface Plasmon resonance (SPR). Characterization of CuO-NPs was carried out by SEM, TEM, EDX, and XRD. However, the action of prepared nanocomposite hydrogels swelling was examined at different pH and saline solutions. In addition, CuO-NPs were also tested using the disk plate diffusion method by means of adverse effect of bacteria Gram-positive bacteria (Bacillus subtilis) as well as Gram-negative bacteria (Escherichia coli). The CuO-NPs hydrogels isolated from lab-made CMC (made from sugarcane bagasse) indicates that the Bacillus subtilis as well as Escherichia coli have greater antibacterial activity compared with synthetic CMC purchase from the market. CuO-NPs can be used effectively in biomedical applications with the production of hydrogels.

Predicting the characteristics of copper oxide nanoparticles using Nyctanthes arbor-tristis flower extract via antibacterial study

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Jothi M ◽  
Aparna Dhevi S
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Morphological Characteristics ◽  
Copper Oxide Nanoparticles ◽  
Nano Particles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Average Crystalline Size ◽  
X Rays ◽  
Monoclinic Crystal

Copper oxide (CuO) is an inorganic compound with monoclinic crystal structure. CuO nano particles attracted considerable attention due to its numerous applications in the field of optical, catalytic, mechanical, organic dye degeneration, biomedicine, pharmaceutics,cosmetics and different medical purposes. In the present study, copper oxide nanoparticles have been prepared by biological method using the flower extracts of Nyctanthes arbortristis as a reducing agent. The resulting samples were characterized using X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive analysis of X-rays (EDX), UV-Visible Spectroscopy (UV) and Antibacterial activity. From XRD studies, the average crystalline size of the obtained sample was calculated by Debye-Scherrer formula and it was found to be 33.13 nm. The band gap energy of the synthesized nano particle was estimated from UV studies and its value is 1.19 eV. The morphological characteristics were absorbed by SEM studies. The EDX and FTIR studies confirm the presence of Copper Oxide nano particles.The antibacterial activity of CuO-nano particles on selected bacteria was done using agar diffusion method.

The impact of copper oxide nanoparticles on the structure and applicability of bioactive glasses

2019 ◽  
Vol 91 (3) ◽  
pp. 634-643 ◽  
Author(s):  
K. Magyari ◽  
Zs. Pap ◽  
Z. R. Tóth ◽  
Zs. Kása ◽  
E. Licarete ◽  
...  
Keyword(s):  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Bioactive Glasses ◽  
The Impact

scholarly journals Anticancer Activity of Copper Oxide Nanoparticles Synthesized from Brassia actinophylla Flower Extract

2019 ◽  
Vol 31 (9) ◽  
pp. 1899-1904
Author(s):  
K. Subashini ◽  
S. Prakash ◽  
V. Sujatha
Keyword(s):  
Metal Oxide ◽  
Copper Oxide ◽  
Anticancer Activity ◽  
Copper Oxide Nanoparticles ◽  
Cuo Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Solution Combustion ◽  
Flower Extract ◽  
Edax Analysis

There are many methods to synthesize metal and metal oxide nanoparticles. In this paper, copper oxide nanoparticles have been synthesized by solution combustion method using Brassia actinophylla i.e. Schefflera actinophylla flower extract belongs to Araliaceae family. The importance of solution combustion is one of the easy and simplest methods for the synthesis of metal oxide nanoparticle. The CuO nanoparticles were synthesized at various temperatures and the characterization has been carried out by UV, FTIR, PXRD, SEM, TEM and EDAX analysis. At lower temperature, the peak was not observed but at 400 ºC, the UV peak was observed at 340 nm. The FTIR peaks observed at 1000-500 cm-1 confirms again the presence of CuO nanoparticles. The monoclinic phase and crystalline structure of nanoparticles were revealed by PXRD pattern, by Scherrer′s method the average crystalline sizes were found to be in the range of 15 to 24 nm. The size and the shape of nanoparticles were confirmed by SEM and TEM reports. The SEM images of nanoparticles show spherical in shape and free from agglomeration. TEM analysis reports the nanoparticle sizes ranging from 2 to 20 nm. The percentage of copper (52 %) and oxygen (26 %) elements were recorded in the EDAX analysis. The study of size and stability of nanoparticles were done by zeta potential values. The antibacterial activity of CuO nanoparticles were carried out against Staphylococcus aureus and Escherichia coli bacteria's by agar well diffusion method. The MTT assay was performed in order to check the anticancer activity of CuO nanoparticles against HT-29 colon cancer cells.

Impediment to growth and yeast-to-hyphae transition in Candida albicans by copper oxide nanoparticles

Biofouling ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 56-72 ◽  
Author(s):  
Alwar Ramanujam Padmavathi ◽  
Sriyutha Murthy P. ◽  
Arindam Das ◽  
Arumugam Priya ◽  
T. J. Sushmitha ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles

scholarly journals Promising Antibacterial Effect of Copper Oxide Nanoparticles against Several Multidrug Resistant Uropathogens

2018 ◽  
Vol 24 (3) ◽  
pp. 213-218 ◽  
Author(s):  
Hadi Sedigh Ebrahim-Saraie ◽  
Hamid Heidari ◽  
Vahid Rezaei ◽  
Seyed Mohammad Javad Mortazavi ◽  
Mohammad Motamedifar
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Multidrug Resistant ◽  
Copper Oxide Nanoparticles ◽  
Diffusion Method ◽  
Multiple Drug ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Conventional Antibiotics

Background: Recently, nanotechnology has been demonstrated to be a promising application to overcome the problem of antibiotic resistance. In the present study, we aimed to determine the antibacterial activity of copper oxide nanoparticles (CuO NPs) on several multiple-drug resistant (MDR) uropathogenic strains. Methods: This in vitro case-control study was performed on 4 uropathogenic bacteria including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa. The antibacterial property was evaluated by well diffusion method at different concentrations of CuO NPs. Results: Overall, NPs concentration of 10, 25 and 50 µg/mL showed the remarkable antibacterial activity. A lower effect was seen against S. aureus strains. CuO NPs exhibited maximum bacterial growth inhibition against E. faecalis strains. In most of the cases, the zone of inhibition in 50 µg/mL concentration was closest to control positive antibiotics. Conclusion: In summary, CuO NPs as an alternative to conventional antibiotics that are currently used showed dose-dependent on antibacterial activity against different uropathogens, specificity towards pathogenic Gram-positive bacteria. This promising antibacterial activity of CuO NPs suggesting the development of NPs coatings on the different surface of biomedical materials for applications in different antimicrobial control systems.

Green Synthesis of Copper Oxide Nanoparticles (CuO NPs) From Aqueous Extract of Seeds of Eletteria Cardamomum and Its Antimicrobial Activity against Pathogens

2020 ◽  
Vol 09 ◽  
Author(s):  
Akshaya Venkatramanan ◽  
Akila Ilangovan ◽  
Pakutharivu Thangarajan ◽  
Anitha Saravanan ◽  
Balachandar M
Keyword(s):  
Antimicrobial Activity ◽  
Copper Oxide ◽  
Green Synthesis ◽  
Aqueous Extract ◽  
Dye Sensitized Solar Cells ◽  
Copper Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Vis Spectroscopy

Background: The Nanomaterials/Nanoparticles are of great interest today because of their small size and large surface area, modular and easily tunable morphology and size. Copper oxide (CuO) nanoparticles are widely used in dye-sensitized solar cells (DSSCs). Research on the synthesis and properties of metallic nanomaterials is a growing field of nanotechnology due to the use of nanoparticles in the scientific, technical, pharmaceutical, and biomedical fields. Green synthesis is an emerging technology for the production of nanoparticles due to its many advantages over traditional physical processes and the method of chemical synthesis. Methods: In this study, we report the cost-effective, long-lasting, stable, and regenerative aqueous extract of Eletteria cardamom seeds to target the synthesis of copper oxide nanoparticles (CuO NPs). This method is completely green, free from toxic and harmful solvents. CuO NPs were synthesized from a cupric nitrate mixture and the aqueous extracts of Eletteria cardamom seeds were kept at room temperature for 24 h. CuO NPs were characterized using UV-visible spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD), dynamic light scattering (DLS), and Fourier Transfer infra-red spectroscopy (FTIR) analyzes. UV - Vis spectroscopy revealed the presence of CuO NPs. Results: SEM images stated that the particles were spherical and ranged in size from 1–100nm. FTIR spectra of control (seed extract) and synthesized CuO NPs identify functional groups of active components. In addition, the synthesized CuO NPs were tested for antimicrobial activity by standard disc diffusion method. Conclusion: Nanoparticles found that Escherichia coli and Staphylococcus aureus resistant areas were observed around each well with antimicrobial activity against disease-causing pathogenic strains.

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