scholarly journals Edible plant-derived essential oils synergistically enhance the Th1, Th2 and anti-inflammatory cytokines in neonatal cord blood monocytic cell line

2017 ◽  
Vol 29 (1) ◽  
pp. 346-357 ◽  
Author(s):  
Swagatika Dash ◽  
Monalisa Ray ◽  
Reena Parida ◽  
K. Gopinath Achary ◽  
Sanghamitra Nayak ◽  
...  
Keyword(s):  
Essential Oils ◽  
Cord Blood ◽  
Cell Line ◽  
Inflammatory Cytokines ◽  
Edible Plant ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Anti Inflammatory

The effect of an anti-inflammatory pentapeptide produced by Entamoeba histolytica on gene expression in the U-937 monocytic cell line

2008 ◽  
Vol 57 (4) ◽  
pp. 145-150 ◽  
Author(s):  
R. Silva-García ◽  
I. Estrada-García ◽  
R. Ramos-Payán ◽  
A. Torres-Salazar ◽  
M. E. Morales-Martínez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Entamoeba Histolytica ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Anti Inflammatory

scholarly journals Anti-SASP and anti-inflammatory activity of resveratrol, curcumin and β-caryophyllene association on human endothelial and monocytic cells

2021 ◽  
Author(s):  
Giulia Matacchione ◽  
Felicia Gurău ◽  
Andrea Silvestrini ◽  
Mattia Tiboni ◽  
Luca Mancini ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Cell Line ◽  
Nutritional Supplement ◽  
Natural Compounds ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Expression Levels ◽  
Cellular Models ◽  
Research Fields ◽  
Anti Inflammatory

AbstractA challenging and promising new branch of aging-related research fields is the identification of natural compounds able to modulate the senescence-associated secretory phenotype (SASP), which characterizes senescent cells and can contribute to fuel the inflammaging. We investigated both the anti-SASP and anti-inflammatory activities of a nutritional supplement, namely Fenoxidol™, composed of turmeric extract bioCurcumin (bCUR), Polydatin (the natural glycosylated precursor of Resveratrol-RSV), and liposomal β-caryophyllene (BCP), in two human cellular models, such as the primary endothelial cell line, HUVECs and the monocytic cell line, THP-1. Replicative and Doxorubicin-induced senescent HUVECs, both chosen as cellular models of SASP, and lipopolysaccharides (LPS)-stimulated THP-1, selected as a model of the inflammatory response, were treated with the three single natural compounds or with a combination of them (MIX). In both senescent HUVEC models, MIX treatment significantly reduced IL-1β and IL-6 expression levels and p16ink4a protein, and also increased SIRT1 protein level, as well as downregulated miR-146a and miR-21 expression, two of the so-called inflamma-miRNAs, more effectively than the single compounds. In THP-1 cells stimulated with LPS, the MIX showed a significant effect in decreasing IL-1β, IL-6, TNF-α, and miR-146a expression levels and Caspase-1 activation, in association with an up-regulation of SIRT1 protein, compared to the single compounds. Overall, our results suggest that the three analysed compounds can have a combined effect in restraining SASP in senescent HUVECs as well as the inflammatory response in LPS-stimulated THP-1 cells.

Inhibition of l-leucine methyl ester mediated killing of THP-1, a human monocytic cell line, by a new anti-inflammatory drug, T614

2000 ◽  
Vol 49 (3) ◽  
pp. 285-294 ◽  
Author(s):  
Tetsuji Sawada ◽  
Shiori Hashimoto ◽  
Shigeto Tohma ◽  
Yuichi Nishioka ◽  
Tatsuo Nagai ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Cell Line ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Inflammatory Drug ◽  
Human Monocytic Cell Line ◽  
Human Monocytic Cell ◽  
Anti Inflammatory

Host Cell Dependence of Human Immunodeficiency Virus Type-1 Drug Resistance Profiles and Tissue Culture Selection Patterns

1995 ◽  
Vol 6 (4) ◽  
pp. 222-229 ◽  
Author(s):  
H. Salomon ◽  
Z. Gu ◽  
Q. Gao ◽  
K. Nagai ◽  
J. Hiscott ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cord Blood ◽  
Cell Line ◽  
Mononuclear Cells ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Immunodeficiency Virus ◽  
Blood Mononuclear Cells ◽  
Cord Blood Mononuclear Cells

Clinical isolates of the human immunodeficiency virus type 1 (HIV-1) displayed differential sensitivity to antiviral nucleosides depending on the type of host cell employed for viral propagation. Viruses derived from the peripheral blood mononuclear cells (PBMC) of subjects on prolonged 3′-azido-3′-deoxythymidine (AZT) therapy behaved as AZT-resistant when tested in either cord blood mononuclear cells or MT-4 cells but as relatively drug-sensitive in the U-937 monocytic cell line. Viruses derived from monocytes/ macrophages of the same individuals behaved as drug-sensitive in all cells tested. It was also shown that cloned recombinant viruses, which contained defined resistance-conferring mutations at either position 65 or 184 in the HIV pol gene, were generally less susceptible to each of 2′-3′-dideoxyinosine (ddl), 2′,3′-dideoxycytidine (ddC) and the (-)enantiomer of 2′,3′-dideoxy-3′thiacytidine (3TC) in MT-4 cells than in any of PBMC, cord blood mononuclear cells (CBMC) or Jurkat cells. Finally, resistance against each of AZT, ddl and ddC could be selected for more easily using MT-4 cells than CBMC or Jurkat lymphocytes and not at all with the U-937 monocytic cell line.

scholarly journals Cytokine Expression by Human Macrophage-Like Cells Derived from the Monocytic Cell Line THP-1 Differs between Treatment with Milk from Preterm- and Term-Delivering Mothers and Pasteurized Donor Milk

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2376
Author(s):  
Veronique Demers-Mathieu ◽  
Robert K. Huston ◽  
David C. Dallas
Keyword(s):  
Gene Expression ◽  
Steady State ◽  
Cell Line ◽  
Inflammatory Cytokines ◽  
Human Macrophage ◽  
Activated Macrophages ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Donor Milk ◽  
Tnf Α

Immunomodulatory proteins from human milk may enhance the protection and development of the infant’s gut. This study compared the immunomodulatory effects of treatment with milk from preterm-(PM) and term-delivering (TM) mothers and pasteurized donor milk (DM) on cytokine gene expression in human macrophage-like cells derived from the monocytic cell line THP-1. The gene expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-12 (p40), IL-10 and GAPDH in macrophages treated with PM, TM and DM at steady and activated (inflammatory) states were measured using real-time reverse transcription-polymerase chain reaction. TNF-α and IL-6 in macrophages (both states) with DM were higher than PM or TM. IL-10 in steady state macrophages with DM was higher than PM whereas DM increased IL-10 in activated macrophages compared with TM. TM increased IL-6 and IL-12 (p40) in steady state macrophages compared with PM. IL-12 (p40) in activated macrophages with TM was higher than PM. IL-10 in steady state macrophages with TM was higher than PM. These results suggest that DM induces higher gene expression of pro-inflammatory and anti-inflammatory cytokines in macrophages compared with PM or TM. PM reduced gene expression of pro-inflammatory cytokines compared with TM, which may decrease the development of necrotizing enterocolitis and systematic inflammation.

scholarly journals Interleukin-10 Modulates Proinflammatory Cytokines in the Human Monocytic Cell Line THP-1 Stimulated withBorrelia burgdorferi Lipoproteins

2000 ◽  
Vol 68 (12) ◽  
pp. 6663-6669 ◽  
Author(s):  
P. K. Murthy ◽  
Vida A. Dennis ◽  
Barbara L. Lasater ◽  
Mario T. Philipp
Keyword(s):  
Cell Line ◽  
Inflammatory Cytokines ◽  
Cytokine Production ◽  
Interleukin 10 ◽  
Monocytic Cell ◽  
Monocytic Cell Line ◽  
Human Monocytic Cell Line ◽  
Tnf Α ◽  
Human Monocytic Cell ◽  
Kinetics Of

ABSTRACT We determined previously that lipoproteins of Borrelia burgdorferi stimulate inflammatory and anti-inflammatory cytokines (interleukin-10 [IL-10]) in monocytes. IL-10 could have an effect on innate and acquired immune responses to B. burgdorferi and influence the magnitude of the infectious inoculum and disease outcome. To understand the mechanism(s) of IL-10 action during early infection, when innate immunity expressed chiefly by skin macrophages is key, we investigated the effect of exogenous and endogenous IL-10 on the production of the macrophage-derived cytokines IL-6, IL-1β, IL-12, and tumor necrosis factor alpha (TNF-α). We used the THP-1 human monocytic cell line and recombinant lipidated OspA (L-OspA) as the model target cell and stimulant, respectively. To determine the kinetics of cytokine production by THP-1 cells, we stimulated them with L-OspA and also with heat-killed B. burgdorferi cells (HBb) and lipopolysaccharide (LPS). Exogenous IL-10 dampened production of inflammatory cytokines, as elicited by lipoproteins. The inhibition of endogenous IL-10 function by anti-IL-10 antibody reduced the production of IL-12 and IL-6 but not that of IL-1β and TNF-α. An inspection of the kinetics of cytokine production clarified this finding. TNF-α was produced prior to, and IL-β was produced at the same time as, IL-10, whereas IL-6 and IL-12 were produced later. HBb, LPS, and L-OspA yielded similar kinetics of cytokine production. This result reinforces the notion that lipoproteins are the functional molecules in HBb and perhaps in vivo. It indicates also that signaling pathways utilized by LPS and lipoproteins may be extensively shared. The results are consistent with a major role played by IL-10 in controlling the initial phase of infection with this spirochete.

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