Investigation of Insecticidal Activity of Blend of Essential Oil of Cymbopogon schoenanthus and neem oil on Plutella xylostella (Lepidoptera: Plutellidae)

2016 ◽  
Vol 19 (6) ◽  
pp. 1478-1486 ◽  
Author(s):  
Lankondjoa Kolani ◽  
Komla Sanda ◽  
Komi Agboka ◽  
Gbénonchi Mawussi ◽  
Koffi Koba ◽  
...  
Toxins ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 842
Author(s):  
Xing Huang ◽  
Yulin Huang ◽  
Chunyue Yang ◽  
Tiantian Liu ◽  
Xing Liu ◽  
...  

Many plants show significant biological activity against pests due to their unique chemical constituents. It is important to identify effective constituents for their development and utilization as botanical pesticides. Our previous study showed that Artemisia lavandulaefolia essential oil had biological activity against Plutella xylostella. Here, we isolated and identified the constituents of essential oil from A. lavandulaefolia by silica gel column chromatography. The main constituents identified were eucalyptol and caryophyllene oxide, and they were confirmed by gas chromatography–mass spectrometry (GC–MS). Eucalyptol and caryophyllene oxide showed strong contact toxicity against P. xylostella larvae after 24 h of application (Median lethal dose, LD50 = 76.97 μL/mL and 20.71 mg/mL. Furthermore, the two active constituents against P. xylostella adults showed significant fumigant activity (Mmedian lethal concentration, LC50 = 3.25 μL/L and 1.06 mg/L, respectively. Finally, we measured the detoxification enzymes and acetylcholinesterase of the larvae treated with active constituents. The eucalyptol-treated larvae displayed enhanced carboxylesterase (CarE) and glutathione-S-transferase (GST) activities in an in vivo experiment, but it was lower for acetylcholinesterase (AchE) activity. The activities of the CarE and GST significantly decreased when exposed to caryophyllene oxide. In general, the two active constituents, eucalyptol and caryophyllene oxide, showed high insecticidal activity, which demonstrates their potential to be used as natural insecticides.


2013 ◽  
Vol 37 (2) ◽  
pp. 138-144 ◽  
Author(s):  
Patrícia Fontes Pinheiro ◽  
Vagner Tebaldi de Queiroz ◽  
Vando Miossi Rondelli ◽  
Adilson Vidal Costa ◽  
Tiago de Paula Marcelino ◽  
...  

The thrips, Frankliniella schultzei, and green peach aphid, Myzus persicae, cause direct damage to plants of economic importance and transmit phytoviruses, causing large economic losses. Chemical constituents of essential oils present a wide range of biological activities. The aim of this work was to evaluate insecticidal activity of essential oil from citronella grass, Cymbopogon winterianus, on F. schultzei and M. persicae. This essential oil was obtained by steam distillation and components were identified by GC/FID and GC/MS. A Potter spray tower was used to spray insects with the essential oil. The major constituents are geraniol (28.62%), citronellal (23.62%) and citronellol (17.10%). Essential oil of C. winterianus at 1% (w v-1) causes mortality in F. schultzei and M. persicae at 34.3% and 96.9%, respectively. The LC50 value for M. persicae was 0.36% and LC90 0.66%. Thus, citronella grass essential oil at 1% (w v-1) is more toxic to M. persicae than F. schultzei. This essential oil shows promise for developing pesticides to manage M. persicae.


2015 ◽  
Vol 17 (4 suppl 1) ◽  
pp. 769-773 ◽  
Author(s):  
R.L. CANSIAN ◽  
V. ASTOLFI ◽  
R.I. CARDOSO ◽  
N. PAROUL ◽  
S.S. ROMAN ◽  
...  

ABSTRACT The aim of this work was to evaluate the insecticidal and repellency activity of the essential oil of Cinnamomum camphora var. linaloolifera Y. Fujita (Ho-Sho) and Cinnamomumcamphora (L.) J Presl.var. hosyo (Hon-Sho), against the Sitophilus zeamais in maize grains. The essential oils were obtained by hydrodistillation and analyzed by GC-MS.The insecticidal activity was determined by the toxicity of different concentrations of essential oils during 24 hours of contact with the insects, in the absence of feed substrate. The Bioassays of repellency were conducted with lethal doses (LD50,LD25,and LD12.5) obtained from insecticidal bioassay. In order to compare the treatments the preference index (PI) was employed. The analysis of the essential oils of Cinnamomum camphora leaves indicated 68% of camphor and 9% of linalool for the variation Hon-Sho and 95% of linalool to the variation Ho-Sho. The variation Ho-Sho presented greatest insecticidal activity than the variation Hon-Sho against the Sitophiluszeamais, with LD50 of 0.35 μL/cm2, whereas in the variation Hon-Sho the ratewas 0.48 μL/cm2. However, considering only the concentrations of linalool and camphor of Ho-Sho and Hon-Sho, the lethal doses’ evaluation of these compounds were similar. The values of the preference index ranged from -0.3 to -0.8 for thevariation Ho-Sho and -0.2 to -0.7 for the variation Hon-Sho. The essential oils evaluated in this work showed repellent activity against Sitophiluszeamais in vitro and in trials performed in mini-silos.


2017 ◽  
Vol 69 (3) ◽  
pp. 409-415 ◽  
Author(s):  
Ivan Pavlovic ◽  
Eihab Omar ◽  
Milica Drobac ◽  
Mirjana Radenkovic ◽  
Suzana Brankovic ◽  
...  

The plants of genus Cymbopogon are well known for their use in traditional medicine and for their high content of essential oils that are widely used as flavoring agents, fragrances, cosmetics, and pharmaceuticals. Essential oils isolated from the dried stems and inflorescence of cultivated Cymbopogon schoenanthus (L.) Spreng. (Poaceae) collected from Khartoum state (Sudan) in two periods were studied. The results of chemical analysis of C. schoenanthus revealed that this plant is rich in essential oil which is a valuable source of the commercially important monoterpene, piperitone (47.7-71.5%). Other abundant constituents of the oils were intermedeol (6.1-17.3%), ?-2-carene (4.5-10.0%) and elemol (2.7-9.0%). The essential oil from the inflorescence was tested for spasmolytic activity using three different experimental models: against spontaneous contractions, contractions induced with acetylcholine and contractions induced with potassium chloride. The oil exhibited strong, significant and dose-dependent spasmolytic activity, indicating the possibility for further investigations of this essential oil for its medicinal purposes or application in food industry.


2016 ◽  
Vol 8 (4) ◽  
pp. 2049-2054 ◽  
Author(s):  
K. K. Chahal ◽  
Ritima Bansal ◽  
Ramandeep Kaur

A laboratory experiment was conducted to study chemistry and insecticidal activity of bay leaf oil, its fractions and isolated compounds against stored grain pest of wheat i.e.Tribolium castaneum Herbst. Bay leaf essential oil extracted from dried and powdered bay leaves was subjected to column chromatography to have its fractions. Extensive column chromatography of polar fraction yielded Eugenol and 7, 7 Dimethyl-3-methylene bicyclo [2.2.1] heptan-4-ol which were identified by spectroscopic techniques. Bay leaf oil was tested for its insecticidal activity at five different concentrations in the range 4-12 mg g-1respectively against F1 generation of red rust flour beetle adults. Maximum inhibition was observed at 12 mg g-1concentration. The activity was both time and concentration dependent. The fractions of bay leaf essential oil and the compounds isolated were tested at 4mg g-1 concentration. Polar fraction was found to be more active as compared to nonpolar fraction as 100 and 53.1% mortality was obtained on 30th day for polar and nonpolar fractions, respectively. Comparison of eugenol and 7, 7 Dimethyl-3-methylene bicyclo [2.2.1] heptan-4-ol showed complete mortality on 33rd and 35thday respectively , which revealed that adults of T. castaneum were more susceptible to eugenol.The results indicated that bay leaf essential oil may have potential to control stored grain pest, T. castaneum.


2008 ◽  
Vol 20 (5) ◽  
pp. 383-385
Author(s):  
Sandra V. Clemente ◽  
Graciela Mareggiani ◽  
Berta E. Juárez ◽  
María E. Mendiondo ◽  
Catalina M. van Baren ◽  
...  

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