Quantification of Genotypic and Chemotypic Diversity for Elite Clone Selection with High-quality Essential Oil Traits in Vetiver [Chrysopogon zizanioides (L.) Roberty]

2019 ◽  
Vol 22 (4) ◽  
pp. 1150-1162
Author(s):  
Namita Singh ◽  
V.R. Singh ◽  
R.K. Lal ◽  
Ram S. Verma ◽  
Anand Mishra ◽  
...  
Keyword(s):  
Essential Oil ◽  
High Quality ◽  
Chrysopogon Zizanioides ◽  
Clone Selection

scholarly journals Influence of cutting type and size on rooting of Lavandula dentata L

2012 ◽  
Vol 14 (1) ◽  
pp. 8-11 ◽  
Author(s):  
C.M. Bona ◽  
I.R. Biasetto ◽  
M. Masetto ◽  
C. Deschamps ◽  
L.A. Biasi
Keyword(s):  
Essential Oil ◽  
Vegetative Propagation ◽  
Plant Material ◽  
The Other ◽  
Root Weight ◽  
Root Number ◽  
Polystyrene Foam ◽  
High Quality ◽  
Cutting Propagation ◽  
Superior Clones

Even though the Lavandula species may be propagated by seeds, it should not be the preferred propagation method because it causes a great lack of uniformity. On the other hand, asexually propagated lavender crops would provide more homogeneous crops, and clones from high quality plant material would increase the odds for obtaining a higher quality essential oil. However, problems such as poor rooting and restrict market availability for superior clones have been a problem in vegetative propagation of the Lavandula species. The objective of this work was to define which type and size of cutting is more adequate for cutting propagation of L. dentata, a very productive Lavandula species. Cuttings with 5, 8, 10 or 13 cm and from the apical or basal parts of stems cut from L. dentata stock plants were placed in Plantmax HT® filled polystyrene foam trays and kept under intermittent mist system for two months. Averages of root number, length of the longest root, fresh and dry root weight, and percentage of rooting were evaluated. Apical cuttings combined 97.9% rooting with an average of 13.2 roots per cutting and basal cuttings 93.7% rooting with 2.98 roots per cutting. Apical cuttings with at least 10 cm in length were considered the most adequate for cutting propagation of L. dentata.

scholarly journals Phytochemical screening, antinociceptive and anti-inflammatory activities of Chrysopogon zizanioides essential oil

2012 ◽  
Vol 22 (2) ◽  
pp. 443-450 ◽  
Author(s):  
Gabrielle M. Lima ◽  
Lucindo J. Quintans-Júnior ◽  
Sara M. Thomazzi ◽  
Emyle M. S. A. Almeida ◽  
Mônica S. Melo ◽  
...  
Keyword(s):  
Essential Oil ◽  
Phytochemical Screening ◽  
Chrysopogon Zizanioides ◽  
Anti Inflammatory

scholarly journals Comparative analysis of the DNA isolated from thyme leaves using different methods

2020 ◽  
Vol 181 (3) ◽  
pp. 155-162
Author(s):  
I. V. Bulavin ◽  
O. A. Grebennikova ◽  
V. A. Brailko ◽  
S. A. Feskov ◽  
I. V. Mitrofanova
Keyword(s):  
Essential Oil ◽  
Cross Sections ◽  
Dna Isolation ◽  
Low Cost ◽  
Automated System ◽  
Ex Situ ◽  
Mass Analysis ◽  
High Quality ◽  
Cheap Method ◽  
Commercial Kits

Background. The base for a molecular analysis is DNA of high quality. For DNA isolation, different kits or classical methods are used. For mass analysis, isolation with kits is a very expensive process. So, the objective of our investigation was to find a cheap method for high-quality DNA isolation from leaves of various thyme cultivars.Materials and methods. Leaves cut from thyme accessions (Thymus mastichina L. cv. ‘Svetliachok’, T. striatus Vahl. cv. ‘Jubileiniy’, T. vulgaris L. cv. ‘Fantasia’, and T. vulgaris cv. ‘Jalos’.) maintained ex situ in the collection of the Nikita Botanical Gardens were used as the material for the analysis. Light microscopy was used to study leaf anatomy and localize essential oil on leaf cross sections. Essential oil was extracted on Ginsberg devices, and phenolic content was measured with The Folin–Ciocâlteu reagent (FCR). Commercial kits (DiamondDNATM, PureLink® Plant Total DNA Purification Kit) and classical methods (CTAB, CTAB with 2% polyvinylpyrrolidone) were used for DNA isolation. DNA quality was evaluated spectrophotometrically, with electrophoresis (horizontal, automated system Agilent 4200 TapeStation) and PCR.Results. The analysis showed that the leaf blade mesophyll of four thyme cultivars had inclusions with essential oil. The content of essential oil and phenolic compounds was measured biochemically. Since the plants were characterized by the presence of secondary metabolites, DNA was isolated by different methods. Spectrophotometry demonstrated that the classical CTAB method and CTAB with 2% PVP provided the best results. Using an automated electrophoresis system, the presence of high-molecularweight DNA (more than 52000 bp) in significant amounts was detected in the samples isolated with DiamondDNATM kit and CTAB + 2% PVP.Conclusion. Among the tested kits and methods, CTAB + 2% PVP provided thyme DNA suitable for PCR and, presumably, for genome library preparation. The low cost of reagents for this technique makes it applicable for future mass analysis of plant material. 

Research note: Micropropagation of Eucalyptus polybractea selected for key essential oil traits

2008 ◽  
Vol 35 (3) ◽  
pp. 247 ◽  
Author(s):  
Jason Q. D. Goodger ◽  
Allison M. Heskes ◽  
Drew J. King ◽  
Roslyn M. Gleadow ◽  
Ian E. Woodrow
Keyword(s):  
Essential Oil ◽  
Shoot Proliferation ◽  
Research Note ◽  
Axillary Bud ◽  
High Quality ◽  
Physiological Constraints ◽  
Axillary Bud Proliferation ◽  
Explant Source ◽  
Significant Difference ◽  
Leaf Oil

A protocol for the micropropagation of Eucalyptus polybractea R.T. Baker (blue mallee) using axillary bud proliferation from lignotuber-derived explants is described. Three different ages of plants were used as explant sources: glasshouse-grown seedlings, field-grown saplings, and coppice of field-grown mature lignotubers. Explants from each source initiated successfully and no significant difference was observed for shoot proliferation, rooting success or hardening success between explant sources. Leaf oil quantity and quality for hardened clones transplanted to a field plantation were assessed after 3 months of growth. Ramets of all clones contained high quality oil with over 80% 1,8-cineole. For seedling-derived clones, foliar oil concentrations of ramets were higher than those of the ortets from which they were derived. For sapling and mature lignotuber derived clones the opposite was the case. This suggests that ontogenetic and physiological constraints may be influencing yield in the young ramets. The age of the explant source did not appear to influence the success of micropropagation, and as a result older plants (for which key oil traits are known) can be selected as elite plants for multiplying selected genotypes via micropropagation.

scholarly journals Essential oil content and composition of lavender origins, introduced in Bulgaria

2021 ◽  
Vol 13 (31) ◽  
pp. 23-25
Author(s):  
Ana Dobreva ◽  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Oil Content ◽  
Linalyl Acetate ◽  
High Quality ◽  
Selection Program ◽  
Three Samples ◽  
The Common ◽  
Qualitative Characteristics

The genus Lavandula includes a variety of species under the common name lavender. Bulgaria has a tradition in the production of high-quality lavender oil. This is the result of many years of selection work, which includes the study of introduced samples and their adaptogenic abilities in the country habitat. Three samples of Lavandula angustifolia Mill., that originated from Poland were studied and compared with the Bulgarian varieties “Hemus” and “Sevtopolis”. The content of the essential oils ranged from 0.39% to 3.98%. The chemical composition, determined by GC/MS, revealed the main compounds: linalyl acetate (13.0÷44.9 %), linalool (21.8÷42.1%), β-caryophyllene (4.6÷7.4 %), cis-β ocimene (2.8÷10.5 %), lavandulyl acetate (1.9÷4.3%), terpinen-4-ol (0.3÷2.0 %), limonene+1.8 cineole (2.3÷6.0 %) and trans-β ocimene (0.1÷3.8%). The sample with the dark purple florescence showed promising quantitative and qualitative characteristics of the essential oil and can be involved in the selection program for lavender cultivation.

scholarly journals Crop Yield and Essential Oil Composition of Two Thymus vulgaris Chemotypes along Three Years of Organic Cultivation in a Hilly Area of Central Italy

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5109
Author(s):  
Basma Najar ◽  
Luisa Pistelli ◽  
Benedetta Ferri ◽  
Luciana Gabriella Angelini ◽  
Silvia Tavarini
Keyword(s):  
Essential Oil ◽  
Central Italy ◽  
Raw Material ◽  
Major Constituent ◽  
Thymus Vulgaris ◽  
Oil Composition ◽  
Medicinal And Aromatic Plants ◽  
Vegetative Development ◽  
High Quality ◽  
Organic Cultivation

Thymus vulgaris L. is one of the most commonly used medicinal and aromatic plants (MAPs), owing to a range of therapeutic properties of its essential oil. Plant growth, biomass yield, essential oil content and composition are influenced by chemotype, environmental conditions, cultivation techniques and vegetative development. Since in MAPs cultivation special attention is paid on high quality of raw material, the adoption of sustainable agriculture methods is of pivotal importance. Therefore, we evaluated the agronomic and qualitative performances of two Thymus vulgaris L. chemotypes, organically cultivated under the Mediterranean climate of hilly lands of central Italy for three consecutive years (2017–2019). Along the trial, total above-ground dry biomass significantly increased from the 1st to 3rd year after planting and large variations in the main biological, biometric and productive traits were observed between the two chemotypes. The ‘thymol’ chemotype EO obviously showed thymol as the major constituent (51.26–49.87%) followed by γ-terpinene and p-cymene. The ‘linalool’ chemotype EO showed high percentages of oxygenated monoterpenes (about 90%) with linalool (75%), linalyl acetate (8.15%) and b-caryophyllene (3.2%) as main constituents. This study highlighted that T. vulgaris can be successfully organically grown in the hilly lands of Tuscany, with interesting biomass and essential oil yields, even though the plants were in the initial years of crop establishment (start in 2017). The introduction of this species into organic cultivation systems could contribute to obtain high-quality raw material, as well as to enhance crop rotation diversification, which is of pivotal importance in the management of organic farms.

Ontogenesis and harvest time are crucial for high quality lavender – Role of the flower development in essential oil properties

2021 ◽  
Vol 163 ◽  
pp. 113334
Author(s):  
E. Détár ◽  
É. Zámbori-Németh ◽  
B. Gosztola ◽  
A. Harmath ◽  
M. Ladányi ◽  
...  
Keyword(s):  
Essential Oil ◽  
Flower Development ◽  
Harvest Time ◽  
High Quality
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