Column chromatography for preparing rosmarinic acid rich extract from Orthosiphon aristatus

SummaryMetabolism and kinetics of 3H-heparin were compared in intact and hepatectomized rats. Rats were divided into three groups: 1) intact rats with biliary fistulas and cystostomies 2) intact rats with only cystostomies and 3) hepatectomized rats with cystostomies. Radioactivity in blood, bile and urine besides anticoagulant activity in blood and urine were examined. In addition, column chromatography of urine was used to isolate possible metabolites. Seventy percent and 80% of the radioactive dose was found in the urine of intact rats at 24 hr and 48 hr. Close to 5% of the radioactivity was found in bile or rats with a biliary fistula after 48 hr. The APTT declined to near normal values at 1 hr whether rats had a biliary fistula or not. In contrast, only 25 % of the radioactivity could be excreted into the urine of hepatectomized rats in 24 hr; the APTT did not decline as fast and at 5 hr, it was still 100 seconds. Only one radioactive component could be isolated on chromatography from all urines of these animals and appears to be similar to the original heparin. Thus, the liver has an important role to play in regulating the anticoagulant effects and excretion of heparin.

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VANILLINSÄURE ALS ENDPRODUKT DES STOFFWECHSELS VON ADRENALIN UND NORADRENALIN. II.

Acta Endocrinologica ◽

10.1530/acta.0.0440101 ◽

1963 ◽

Vol 44 (1) ◽

pp. 101-106 ◽

Cited By ~ 4

Author(s):

Wilhelm Dirscherl ◽

Helmut Thomas

Keyword(s):

Rat Liver ◽

Paper Chromatography ◽

Column Chromatography ◽

Vanillic Acid ◽

Hippuric Acid ◽

Single Spot ◽

Solvent Systems ◽

Perfusion Experiment ◽

Kinetics Of

ABSTRACT Perfusion of rat liver with vanillic acid yielded only one metabolite. In paper chromatography with three different solvent systems, the substance showed the same RF-values as vanillyolglycine (3-methoxy-4-hydroxyhippuric acid) and in mixed chromatograms there was only one single spot. After separation by column chromatography, the UV- and IRspectra of the reaction product were identical with those of 3-methoxy4-hydroxy-hippuric acid. During the perfusion experiment, the kinetics of the conjugation were investigated.

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DIE ZUSAMMENSETZUNG DER JODHALTIGEN VERBINDUNGEN UND IHRE BEZIEHUNG ZUM JODUMSATZ IN EUTHYREOTEN STRUMEN

Acta Endocrinologica ◽

10.1530/acta.0.0410584 ◽

1962 ◽

Vol 41 (4) ◽

pp. 584-594 ◽

Cited By ~ 2

Author(s):

Dankwart Reinwein ◽

Erich Klein

Keyword(s):

Column Chromatography ◽

Iodine Content ◽

High Plasma ◽

Secretion Rate ◽

Total Iodine ◽

Iodine Compound ◽

Hormonal Secretion ◽

Carrier Free ◽

Iodine Metabolism ◽

Diffuse Goiter

ABSTRACT The iodine metabolism was investigated in 29 patients with euthyroid non-endemic diffuse goiter. 1 to 14 days before thyroidectomy the patients received carrier-free 131I. The chemical iodine fractions (PBI, BEI and iodide) of the thyroid and the labelled iodine compound were analyzed by means of paper- and column chromatography. In one gland the total iodine content varied only by ± 19.6% of the average, the relative shares of PBI, BEI and iodide as well as that of the iodoamino acids being equal. Monoiodotyrosine, diiodotyrosine and thyroxine were found in the thyroid homogenate without hydrolysis. The homogenate after hydrolysis contained more iodotyrosines at the expense of iodothyronines than do normal glands. 17 goiters with normal 131I-uptake showed a high total iodine content (14.2 ± 5.0 mg) whilst 9 goiters with an increased 131I-uptake had low values (3.58 ± 0.6 mg). The opposite was found for the relative shares of BEI with the chromatographically isolated iodothyronines thyroxine, triiodothyronine and an unidentified iodine compound. Goiters with »high plasma PB131I« were characterized by a faster transfer of 131I into the more heavily iodinated compounds than is found in glands with a normal hormonal secretion rate. The highest values for the iodothyronines were found in goiters with increased 131I-uptake together with a high hormonal secretion rate. From this study it appears that the changes in the iodine-poor glands are due to a defective exo- or endogenous iodine supply. The observed alterations in iodine-rich glands are probably induced by a faulty iodine utilization characterized by an incomplete iodotyrosyl-coupling defect.

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Protein Solvent Interaction: Transition of Protein-solvent Interaction Concept from Basic Research into Solvent Manipulation of Chromatography

Current Protein and Peptide Science ◽

10.2174/1389203718666171024121529 ◽

2018 ◽

Vol 20 (1) ◽

pp. 34-39 ◽

Cited By ~ 1

Author(s):

Tsutomu Arakawa ◽

Yoshiko Kita

Keyword(s):

Column Chromatography ◽

Rapid Development ◽

Academic Research ◽

Basic Research ◽

Protein Stabilization ◽

Special Issue ◽

Solvent Interaction ◽

Protein Formulations ◽

Solvent Additive ◽

Downstream Processes

Previously, we have reviewed in this journal (Arakawa, T., Kita, Y., Curr. Protein Pept. Sci., 15, 608-620, 2014) the interaction of arginine with proteins and various applications of this solvent additive in the area of protein formulations and downstream processes. In this special issue, we expand the concept of protein-solvent interaction into the analysis of the effects of solvent additives on various column chromatography, including mixed-mode chromatography. Earlier in our research, we have studied the interactions of such a variety of solvent additives as sugars, salts, amino acids, polymers and organic solvents with a variety of proteins, which resulted in mechanistic understanding on their protein stabilization and precipitation effects, the latter known as Hofmeister series. While such a study was then a pure academic research, rapid development of genetic engineering technologies and resultant biotechnologies made it a valuable knowledge in fully utilizing solvent additives in manipulation of protein solution, including column chromatography.

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