scholarly journals Antioxidant capacity and characteristics of theaflavin catechins and ginger freeze-dried extract as affected by extraction techniques

2021 ◽  
Vol 24 (1) ◽  
pp. 1097-1116
Author(s):  
Ali Imran ◽  
Muhammad Umair Arshad ◽  
Hafsah Sherwani ◽  
Rabia Shabir Ahmad ◽  
Muhammad Sajid Arshad ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Extraction Techniques ◽  
Freeze Dried ◽  
Dried Extract

Alteration in the oxidative status of Drosophila melanogaster Meigen (Diptera: Drosophilidae) fed with a diet containing Centaurea depressa M. Bieb. (Asteraceae)

2020 ◽  
Vol 70 (2) ◽  
pp. 227-237
Author(s):  
Eda Güneş
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Total Protein ◽  
Protein Carbonyl ◽  
Control Group ◽  
Carbonyl Content ◽  
Protein Carbonyl Content ◽  
Freeze Dried ◽  
Dried Extract

Abstract The aim of the this study was to evaluate the effects of fresh, dried and freeze-dried Centaurea depressa M. Bieb. (Asteraceae) on the oxidant and antioxidant status of the model organism D. melanogaster Meigen (Diptera: Drosophilidae) experimentally. The study was carried out from 2016 to 2019, and plant leaf extracts (0-50 mg/l) were added to insect standard artificial diets. The total protein, protein carbonyl content and glutathione-S-transferase, superoxide dismutase and catalase activities were quantified at the insect’s third larval stage. Our data showed that protein carbonyl content varied from 2.70 nmol/mg protein in the control group to 59.11 nmol/mg protein in the group fed with fresh leaf extract signifying induction of oxidative stress. All extracts increased the levels of all antioxidant enzymes and decreased the amounts of total protein. Meanwhile, the group fed with the freeze-dried extract showed no significant difference in the levels of total protein and protein carbonyl content except at the 50 mg/l concentration of the extract. Moreover, this group had superoxide dismutase and catalase activities 4 to 5 times higher than in the control group. In conclusion, induction of oxidative stress indicates that the fresh form of C. depressa leaves may have potential as a natural pesticide, whereas induction of endogenous antioxidant enzymes by the freeze-dried extract suggest its potential as an antioxidant.

PRELIMINARY STUDIES RELATED TO MICROSCOPY AND THE SEDEM EXPERT SYSTEM PROFILE ON FREEZED-DRIED EXTRACT OF LYTHRI HERBA

2021 ◽  
Author(s):  
Valeriu Valeriu Iancu ◽  
Laura Adriana Bucur ◽  
Verginica Schröder ◽  
Manuela Rossemary Apetroaei
Keyword(s):  
Expert System ◽  
Plant Species ◽  
Plant Material ◽  
Lythrum Salicaria ◽  
Total Polyphenols ◽  
Spongy Mesophyll ◽  
Freeze Dried ◽  
Anomocytic Stomata ◽  
Dried Extract ◽  
Antibacterial And Antifungal

"The floral tips of the plant species Lythrum salicaria L. represent a rich source of total polyphenols, among which with the largest share we mention tannins, and this is why this plant material has a standardized monograph in the European Pharmacopoeia 10.0th edition. According to the literature accessed so far, the plant material has antioxidant, anti-inflammatory, hemostatic, antibacterial and antifungal properties, along with modulatory action on carbohydrate metabolism. Powder microscopic examination is an important step in establishing the identity of the plant species used, highlighting elements specific to the aerial part such as spiral vessels of the stem, fragments of the spongy mesophyll with calcium oxalate clusters cells and anomocytic stomata. The application of the SeDeM method on dried plant extracts represents an innovative trend in pharmaceutical technology and contributes to the collection of data in a structured and standardized form. In this paper, the functions and applications of the SeDeM expert system are illustrated upon the freeze-dried extract of Lythri herba for the purpose of easier identification and standardization. Future applications may include obtaining chewable gums or tablets by direct compression."

scholarly journals Citrus limon Extract Loaded in Vesicular Systems for the Protection of Oral Cavity

Medicines ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 108 ◽  
Author(s):  
Maria Manconi ◽  
Maria Manca ◽  
Carla Caddeo ◽  
Giorgia Sarais ◽  
Alessandra Palmieri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lactobacillus Acidophilus ◽  
Freeze Drying ◽  
Citrus Limon ◽  
Antibacterial Effects ◽  
Bacterial Proliferation ◽  
Freeze Dried ◽  
Ethanol Evaporation ◽  
Dried Extract

Background: The nanoincorporation of the extract of Citrus limon (L.) Osbeck var. pompia into liposomes was aimed at improving its antioxidant and antibacterial effects. Methods: The extract of the rind of Citrus limon (L.) Osbeck var. pompia was obtained by maceration in ethanol, evaporation, and freeze-drying. The extract phytochemical fingerprint was obtained by HPLC and mass spectrometry, and it was determined that gallic acid, neohesperidin, eriocitrin, and neoeriocitrin were the most abundant components. The freeze-dried extract was loaded in liposomes, glycerosomes, and penetration-enhancer-containing vesicles prepared with propylene glycol (PG-PEVs). Results: Capability of the vesicles of improving efficacy of the extract in counteracting oxidative stress was studied in vitro in keratinocytes, along with antimicrobial activity against planktonic cultures of Streptococcus mutans, Lactobacillus acidophilus, and Streptococcus sanguinis. Conclusion: Results showed that the vesicles, especially glycerosomes and PG-PEVs, prevented oxidative damage and cell death, and inhibited bacterial proliferation.

scholarly journals Gamma Radiation Applied in Euterpe oleraceae Pulps

2020 ◽  
pp. 134-145
Author(s):  
Lucia Maria Jaeger de Carvalho ◽  
Bruno Paranhos ◽  
Edgar Francisco Oliveira de Jesus ◽  
José Luiz Viana de Carvalho
Keyword(s):  
Antioxidant Capacity ◽  
Rio De Janeiro ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Low Doses ◽  
Plastic Bags ◽  
Freeze Dried ◽  
Total Anthocyanins ◽  
The City ◽  
Nuclear Instrumentation

Aims: The aim of this work was to evaluate the optimal radiation dose to maintain the antioxidant capacity of conventional and organic acaí freeze-dried pulps. Study Design: All analyses were conducted in sextuplicate for each experiment. Place and Duration of Study: Were conducted at the LATAIA and the irradiation processes at the Laboratory of Nuclear Instrumentation, UFRJ, Rio de Janeiro, Brazil. The study was carried out from July, 2018 to March, 2020. Methodology: Frozen acaí pulps from two commercial brands, one of them organic and other conventional were used and purchased in the city of Rio de Janeiro, packed in plastic bags containing 1 kg. For each brand, frozen pulps (5 kg) were thawed at 4ºC, opened and homogenized. Samples were subdivided into aliquots ranging from 20 to 300 g for the assays and frozen inside the plastic bags until analyses. A Cobalt 60, Gammacell irradiator was used and doses of 1.25. 2.5. 3.75 and 5 kGy were applied in the in natura pulps. Antioxidant capacity was performed by the ORAC and DPPH methods and, phenolic compounds by Folin Ciocalteau method and, total anthocyanins and majority anthocyanidins by HPLC. Results: Anthocyanins increases at irradiaton dose up to 3.75 kGy in organic açaí but it was not significant in conventional acaí irradiated at low doses (1.25 to 3.75 kGy). Our results suggested that irradiation doses up to 5 kGy do not decrease total phenolic or anthocyanin contents nor the pulp antioxidant activity compared with non-irradiated pulps. The results showed irradiation did not reduce these analytes, and even increased in the organic acaí. Conclusion: The study evidenced that gamma irradiation can be an alternative safe process for fruit pulps preservation. We conclude that irradiation doses up to 5 kGy can be used in acaí without harming its antioxidant properties.

Pharmaco-technical profile of Lythri herba freeze-dried extract based on SeDeM methodology

2021 ◽  
Author(s):  
Valeriu Iancu ◽  
Irina Mihaela Iancu ◽  
Fiorentina Nicoleta Roncea ◽  
Horatiu Miresan ◽  
Radu George Cazacincu ◽  
...  
Keyword(s):  
Freeze Dried ◽  
Dried Extract

scholarly journals Antioxidant Capacity and the Correlation with Major Phenolic Compounds, Anthocyanin, and Tocopherol Content in Various Extracts from the Wild EdibleBoletus edulisMushroom

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Emanuel Vamanu ◽  
Sultana Nita
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Edible Mushroom ◽  
Tocopherol Content ◽  
Hot Water ◽  
Antioxidant Compounds ◽  
Methanolic Extracts ◽  
Freeze Dried

Boletus edulisis a wild edible mushroom habitually consumed by rural populations. Ethanolic and methanolic extracts was obtained in cold and hot water from dried fruit bodies. The antioxidant activity of freeze-dried extracts fromB. eduliswere investigated using free radicals scavenging activity, reducing power, metal chelating effect, inhibition of lipid peroxidation, and the identification of antioxidant compounds. The levels of different compounds with antioxidant properties were higher in alcoholic extracts compared with aqueous extracts. Rosmarinic acid was the major phenolic compound, it being identified in a concentration between7±0.23and56±0.15 mg/100 g extract. A positive correlation between the content of total phenols, flavonoids, anthocyanins, and tocopherols, and the antioxidant capacity of the extracts was determined. The results showed that the ethanolic extract of Romanian wild mushroomB. edulisrepresents a natural source of functional compounds.

scholarly journals Investigation of the antioxidant capacity of the components of Astragalus after membrane separation

2020 ◽  
Vol 185 ◽  
pp. 04061
Author(s):  
Yandong Wang ◽  
Yafei Guo ◽  
Yingli Wang ◽  
Zemin Zhang
Keyword(s):  
Free Radicals ◽  
Antioxidant Capacity ◽  
Membrane Separation ◽  
Antioxidant Activities ◽  
Dose Effect ◽  
Total Flavonoids ◽  
Astragaloside Iv ◽  
Separation Technology ◽  
Freeze Dried ◽  
Hydroxyl Free Radicals

Astragalus is commonly used in health supplements, and its flavonoids and saponins are the important material basis for immune system enhancement. The study on the effective component contents and antioxidant capacities of astragalus extract after membrane separation lays the foundation of the application of membrane separation technology in health supplement development. The astragalus extract was filtered by suction and passed through membranes of 10000 Da, 2500 Da, and 600 Da to obtain retentate 1 (M1), retentate 2 (M2), retentate 3 (M3) and permeate MT. UV/vis spectrophotometer was used to compare the contents of total flavonoids and total saponins before and after each step of membrane separation. High performance liquid chromatography (HPLC) was used to analyze the contents of Verbasil Glucoside and Astragaloside IV of all membrane separation samples, and the antioxidant activities were determined. The contents of flavonoids in membrane separation samples were significantly different. In the freeze-dried powders obtained from the membrane separation, the contents of total flavonoids and Verbasil Glucoside were the lowest in MT, M1 was the highest, and M3 was the second highest. The order of contents of total saponins and Astragaloside IV of the freeze-dried powders from membrane separation was as follows: M3 > M1 > M2 > MT. Among them, the content of total saponins in the freeze-dried powder of M3 was the highest, which reached 2.67 times of that of the astragalus extract. The order of the scavenging activities of membrane separation samples for DPPH free radical was: MT > M3 > M2 > M1 > astragalus extract. The strongest scavenging activity of hydroxyl free radicals was found in M3, and the scavenging rates of hydroxyl free radicals in all samples were higher than those of VC. The total antioxidant capacity of FRAP showed a certain dose-effect relationship. At the same concentration, the FRAP values of M1 and MT were higher than other samples. Membrane separation technology can be used to separate and purify the effective components from astragalus extract. M3 has the highest contents of the total flavonoids and total saponins, and its antioxidant capacity is better than that of astragalus extract and other samples obtained by membrane separation.

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