One Step RT-PCR for Rapid Detection of Crimean-Congo Haemorrhagic Fever Virus

2008 ◽  
Vol 22 (3) ◽  
pp. 864-866 ◽  
Author(s):  
N. Kalvatchev ◽  
I. Christova
Keyword(s):  
Rapid Detection ◽  
Fever Virus ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Crimean Congo Haemorrhagic Fever ◽  
One Step

scholarly journals Identification of a novel lineage of Crimean–Congo haemorrhagic fever virus in dromedary camels, United Arab Emirates

2020 ◽  
Author(s):  
Abdelmalik I. Khalafalla ◽  
Yan Li ◽  
Anna Uehara ◽  
Nasareldien A. Hussein ◽  
Jing Zhang ◽  
...  
Keyword(s):  
United Arab Emirates ◽  
Asymptomatic Infection ◽  
Fever Virus ◽  
Pcr Analysis ◽  
Molecular Evidence ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Dromedary Camels ◽  
High Fatality Rate ◽  
Crimean Congo Haemorrhagic Fever

Crimean–Congo haemorrhagic fever virus (CCHFV) is a tick-borne virus causing Crimean–Congo haemorrhagic fever (CCHF), a disease reported to have a high fatality rate in numerous countries. The virus is geographically widespread due to its vector, and numerous wild and domestic animals can develop asymptomatic infection. Serological and limited molecular evidence of CCHFV has previously been reported in Camelus dromedarius (the dromedary, or one-humped camel) in the United Arab Emirates (UAE). In this study, 238 camel samples were screened for CCHFV RNA where 16 camel samples were positive for CCHFV by RT-PCR. Analysis of full-length CCHFV genome sequences revealed a novel lineage in camels from the UAE, and potential reassortment of the M segment of the genome.

scholarly journals DETECTION AND GENETIC CHARACTERISTICS OF RNA ISOLATES OF CRIMEAN-CONGO HAEMORRHAGIC FEVER VIRUS FROM THE HYALOMMA MARGINATUM TICKS COLLECTED IN THE ASTRAKHAN REGION (2016)

2017 ◽  
Vol 22 (5) ◽  
pp. 248-253
Author(s):  
Elena V. Vakalova ◽  
A. S Volynkina ◽  
E. S Kotenev ◽  
L. N Kulikova ◽  
N. V Viktorova
Keyword(s):  
Infection Rate ◽  
Genetic Variant ◽  
Fever Virus ◽  
Viral Resistance ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Genetic Characteristics ◽  
Hyalomma Marginatum ◽  
Cchf Virus ◽  
Crimean Congo Haemorrhagic Fever

1,746 specimens of H. marginatum ticks collected in the Astrakhan region were examined by RT-PCR, their infection rate with CCHF virus amounted to 1.5%. This is comparable with results of earlier studies performed in different years in the Astrakhan and Rostov regions and testified to such indices of viral resistance as 0.1; 0.9; 2.4; 0.6; 0.9 and 2.3%. As a result of sequencing of fragments of the genome (fourteen of the 26 isolates of RNA, they refer to the genotype Europe-1. Seven out of 14 isolates belong to the subtype Va Stavropol-Rostov-Astrakhan, two represent the reassortant genetic variant S-Vc; M-Vb; L-Va.

scholarly journals Presence of antibodies to Crimean Congo haemorrhagic fever virus in sheep in Tunisia, North Africa

2021 ◽  
Author(s):  
Médiha Khamassi Khbou ◽  
Rihab Romdhane ◽  
Faten Bouaicha Zaafouri ◽  
Mohsen Bouajila ◽  
Limam Sassi ◽  
...  
Keyword(s):  
North Africa ◽  
Fever Virus ◽  
Haemorrhagic Fever ◽  
Crimean Congo Haemorrhagic Fever

scholarly journals Molecular epidemiology of Crimean-Congo haemorrhagic fever virus in India

2016 ◽  
Vol 144 (16) ◽  
pp. 3422-3425 ◽  
Author(s):  
P. SINGH ◽  
M. CHHABRA ◽  
P. SHARMA ◽  
R. JAISWAL ◽  
G. SINGH ◽  
...  
Keyword(s):  
Eastern Europe ◽  
N Gene ◽  
Western India ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Study Region ◽  
Phylogenetic Relatedness ◽  
Cchf Virus ◽  
Crimean Congo Haemorrhagic Fever ◽  
First Time

SUMMARYCrimean-Congo haemorrhagic fever (CCHF) is an emerging zoonotic disease in India which is prevalent in neighbouring countries. CCHF virus (CCHFV) is a widespread tick-borne virus which is endemic in Africa, Asia, Eastern Europe and the Middle East. In the present study, samples of clinically suspected human cases from different areas of northern-western India were tested for the presence of CCHFV by RT–PCR through amplification of nucleocapsid (N) gene of CCHFV. Positive samples were sequenced to reveal the prevailing CCHFV genotype(s) and phylogenetic relatedness. A phylogenetic tree revealed the emergence of diverse strains in the study region showing maximum identity with the Pakistan, Afghanistan and Iran strains, which was different from earlier reported Indian strains. Our findings reveal for the first time the emergence of the Asia 1 group in India; while earlier reported CCHFV strains belong to the Asia 2 group.

scholarly journals Next-generation sequencing of southern African Crimean-Congo haemorrhagic fever virus isolates reveals a high frequency of M segment reassortment

2014 ◽  
Vol 142 (9) ◽  
pp. 1952-1962 ◽  
Author(s):  
D. GOEDHALS ◽  
P. A. BESTER ◽  
J. T. PAWESKA ◽  
R. SWANEPOEL ◽  
F. J. BURT
Keyword(s):  
Next Generation Sequencing ◽  
High Frequency ◽  
Complete Genome ◽  
Fever Virus ◽  
Likelihood Method ◽  
Haemorrhagic Fever ◽  
Next Generation ◽  
Genome Sequences ◽  
Crimean Congo Haemorrhagic Fever ◽  
Generation Sequencing

SUMMARYCrimean Congo haemorrhagic fever virus (CCHFV) is a bunyavirus with a single-stranded RNA genome consisting of three segments (S, M, L), coding for the nucleocapsid protein, envelope glycoproteins and RNA polymerase, respectively. To date only five complete genome sequences are available from southern African isolates. Complete genome sequences were generated for 10 southern African CCHFV isolates using next-generation sequencing techniques. The maximum-likelihood method was used to generate tree topologies for 15 southern African plus 26 geographically distinct complete sequences from GenBank. M segment reassortment was identified in 10/15 southern African isolates by incongruencies in grouping compared to the S and L segments. These reassortant M segments cluster with isolates from Asia/Middle East, while the S and L segments cluster with strains from South/West Africa. The CCHFV M segment shows a high level of genetic diversity, while the S and L segments appear to co-evolve. The reason for the high frequency of M segment reassortment is not known. It has previously been suggested that M segment reassortment results in a virus with high fitness but a clear role in increased pathogenicity has yet to be shown.

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