scholarly journals DETECTION AND GENETIC CHARACTERISTICS OF RNA ISOLATES OF CRIMEAN-CONGO HAEMORRHAGIC FEVER VIRUS FROM THE HYALOMMA MARGINATUM TICKS COLLECTED IN THE ASTRAKHAN REGION (2016)

2017 ◽  
Vol 22 (5) ◽  
pp. 248-253
Author(s):  
Elena V. Vakalova ◽  
A. S Volynkina ◽  
E. S Kotenev ◽  
L. N Kulikova ◽  
N. V Viktorova
Keyword(s):  
Infection Rate ◽  
Genetic Variant ◽  
Fever Virus ◽  
Viral Resistance ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Genetic Characteristics ◽  
Hyalomma Marginatum ◽  
Cchf Virus ◽  
Crimean Congo Haemorrhagic Fever

1,746 specimens of H. marginatum ticks collected in the Astrakhan region were examined by RT-PCR, their infection rate with CCHF virus amounted to 1.5%. This is comparable with results of earlier studies performed in different years in the Astrakhan and Rostov regions and testified to such indices of viral resistance as 0.1; 0.9; 2.4; 0.6; 0.9 and 2.3%. As a result of sequencing of fragments of the genome (fourteen of the 26 isolates of RNA, they refer to the genotype Europe-1. Seven out of 14 isolates belong to the subtype Va Stavropol-Rostov-Astrakhan, two represent the reassortant genetic variant S-Vc; M-Vb; L-Va.

scholarly journals Molecular epidemiology of Crimean-Congo haemorrhagic fever virus in India

2016 ◽  
Vol 144 (16) ◽  
pp. 3422-3425 ◽  
Author(s):  
P. SINGH ◽  
M. CHHABRA ◽  
P. SHARMA ◽  
R. JAISWAL ◽  
G. SINGH ◽  
...  
Keyword(s):  
Eastern Europe ◽  
N Gene ◽  
Western India ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Study Region ◽  
Phylogenetic Relatedness ◽  
Cchf Virus ◽  
Crimean Congo Haemorrhagic Fever ◽  
First Time

SUMMARYCrimean-Congo haemorrhagic fever (CCHF) is an emerging zoonotic disease in India which is prevalent in neighbouring countries. CCHF virus (CCHFV) is a widespread tick-borne virus which is endemic in Africa, Asia, Eastern Europe and the Middle East. In the present study, samples of clinically suspected human cases from different areas of northern-western India were tested for the presence of CCHFV by RT–PCR through amplification of nucleocapsid (N) gene of CCHFV. Positive samples were sequenced to reveal the prevailing CCHFV genotype(s) and phylogenetic relatedness. A phylogenetic tree revealed the emergence of diverse strains in the study region showing maximum identity with the Pakistan, Afghanistan and Iran strains, which was different from earlier reported Indian strains. Our findings reveal for the first time the emergence of the Asia 1 group in India; while earlier reported CCHFV strains belong to the Asia 2 group.

scholarly journals Haemophagocytosis in a patient with Crimean–Congo haemorrhagic fever

2007 ◽  
Vol 56 (8) ◽  
pp. 1126-1128 ◽  
Author(s):  
Atahan Cagatay ◽  
Mahir Kapmaz ◽  
Asli Karadeniz ◽  
Seniha Basaran ◽  
Mustafa Yenerel ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Severe Disease ◽  
Case Fatality ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Patient History ◽  
Endemic Region ◽  
Cchf Virus ◽  
History Of ◽  
Crimean Congo Haemorrhagic Fever

Crimean–Congo haemorrhagic fever (CCHF) is a severe disease with a case fatality of 2.8 to 80 %. A patient dwelling in an endemic region for CCHF was admitted with fever preceding bleeding diathesis and pancytopenia. Despite no history of tick exposure, CCHF was highly suspected. With an oral ribavirin therapy, clinical and laboratory improvements were obtained. The diagnosis was confirmed by detection of IgM antibody to CCHF virus and positive RT-PCR. Although the main pathogenesis of CCHF infection is not elucidated yet, haemophagocytosis, a symptom rarely reported in viral haemorrhagic fevers, was observed in this case. Haemophagocytosis is suggested to have a role in the development of pancytopenia in CCHF, the mechanism of which still needs to be investigated, probably with cytokine studies. Together with clinical symptoms and patient history, haemophagocytosis may be an indicator for CCHF.

scholarly journals Phylogenetic analysis in a recent controlled outbreak of Crimean-Congo haemorrhagic fever in the south of Iran, December 2008

2010 ◽  
Vol 15 (47) ◽  
Author(s):  
S Chinikar ◽  
S M Ghiasi ◽  
M Moradi ◽  
M M Goya ◽  
M Reza Shirzadi ◽  
...  
Keyword(s):  
Surveillance System ◽  
Phylogenetic Analyses ◽  
Ixodid Ticks ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Source Of Infection ◽  
Cchf Virus ◽  
Control Programmes ◽  
Crimean Congo Haemorrhagic Fever ◽  
And Control

Crimean-Congo haemorrhagic fever (CCHF) is a viral zoonotic disease with a high mortality rate in humans. The CCHF virus is transmitted to humans through the bite of Ixodid ticks or contact with blood or tissues of CCHF patients or infected livestock. In December 2008, a re-emerging outbreak of CCHF occurred in the southern part of Iran. Five people were hospitalised with sudden fever and haemorrhaging, and CCHF was confirmed by RT-PCR and serological assays. One of the cases had a fulminant course and died. Livestock was identified as the source of infection; all animals in the incriminated herd were serologically analysed and more than half of them were positive for CCHFV. We demonstrated that two routes of transmission played a role in this outbreak: contact with tissue and blood of infected livestock, and nosocomial transmission. Phylogenetic analyses helped to identify the origin of this transmission. This outbreak should be considered as a warning for the national CCHF surveillance system to avoid further outbreaks through robust prevention and control programmes.

scholarly journals Identification of a novel lineage of Crimean–Congo haemorrhagic fever virus in dromedary camels, United Arab Emirates

2020 ◽  
Author(s):  
Abdelmalik I. Khalafalla ◽  
Yan Li ◽  
Anna Uehara ◽  
Nasareldien A. Hussein ◽  
Jing Zhang ◽  
...  
Keyword(s):  
United Arab Emirates ◽  
Asymptomatic Infection ◽  
Fever Virus ◽  
Pcr Analysis ◽  
Molecular Evidence ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Dromedary Camels ◽  
High Fatality Rate ◽  
Crimean Congo Haemorrhagic Fever

Crimean–Congo haemorrhagic fever virus (CCHFV) is a tick-borne virus causing Crimean–Congo haemorrhagic fever (CCHF), a disease reported to have a high fatality rate in numerous countries. The virus is geographically widespread due to its vector, and numerous wild and domestic animals can develop asymptomatic infection. Serological and limited molecular evidence of CCHFV has previously been reported in Camelus dromedarius (the dromedary, or one-humped camel) in the United Arab Emirates (UAE). In this study, 238 camel samples were screened for CCHFV RNA where 16 camel samples were positive for CCHFV by RT-PCR. Analysis of full-length CCHFV genome sequences revealed a novel lineage in camels from the UAE, and potential reassortment of the M segment of the genome.

scholarly journals Evidence of segment reassortment in Crimean-Congo haemorrhagic fever virus

2004 ◽  
Vol 85 (10) ◽  
pp. 3059-3070 ◽  
Author(s):  
Roger Hewson ◽  
Anatoly Gmyl ◽  
Larissa Gmyl ◽  
Svetlana E. Smirnova ◽  
Galina Karganova ◽  
...  
Keyword(s):  
Nucleotide Sequences ◽  
Fever Virus ◽  
Full Length ◽  
Haemorrhagic Fever ◽  
First Report ◽  
Large Segment ◽  
Cchf Virus ◽  
Phylogenetic Grouping ◽  
Crimean Congo Haemorrhagic Fever ◽  
Virus Strains

The complete nucleotide sequences of the small (S) and medium (M) segments of three independent strains of Crimean-Congo haemorrhagic fever (CCHF) virus isolated in Uzbekistan, Iraq and Pakistan have been determined. Partial S and M segment sequences from two additional strains and partial large segment sequences from five strains of CCHF virus have also been obtained. These data have been compiled and compared with published full-length and partial sequences of other CCHF virus strains. Analysis of virus strains for which complete and partial S and M segment sequences are available reveals that the phylogenetic grouping of some strains differ between these two segments. Data provided in this report suggest that this discrepancy is not the result of recombination, but rather the consequence of reassortment events that have occurred in some virus lineages. Although described in other genera of the Bunyaviridae family, this is the first report of segment reassortment occurring in the Nairovirus genus.

scholarly journals Adult Hyalomma marginatum tick positive for Rickettsia aeschlimannii in Austria, October 2018

2018 ◽  
Vol 23 (48) ◽  
Author(s):  
Georg Gerhard Duscher ◽  
Adnan Hodžić ◽  
Peter Hufnagl ◽  
Walpurga Wille-Piazzai ◽  
Anna-Magarita Schötta ◽  
...  
Keyword(s):  
Tick Species ◽  
Migratory Birds ◽  
Fever Virus ◽  
Northern Europe ◽  
Haemorrhagic Fever ◽  
Potential Threat ◽  
Hyalomma Marginatum ◽  
Crimean Congo Haemorrhagic Fever ◽  
Rickettsia Aeschlimannii

We report on a non-indigenous adult Hyalomma marginatum tick in Austria carrying the human pathogenic Rickettsia aeschlimannii; presumably introduced as a nymph via migratory birds and completed the moulting within the same year. It was negative for Crimean-Congo haemorrhagic fever virus, but the finding of R. aeschlimannii represents a potential threat for humans due to its zoonotic character. Awareness of invasive tick species and carried pathogens should be improved in central and northern Europe.

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