Assay of salsolinol in peripheral blood mononuclear cells of alcoholics and healthy subjects by gas chromatography-mass spectrometry

Human gut microbes are a profitable tool for the modification of food compounds into biologically active metabolites. The biological properties of catechins have been extensively investigated. However, the bioavailability of catechin in human blood plasma is very low. This study aimed to determine the biotransformed catechin metabolites and their bioactive potentials for modulating the immune response of human peripheral blood mononuclear cells (PBMCs). Biotransformation of catechin was carried out using in-vitro gut microbial biotransformation method, the transformed metabolites were identified and confirmed by gas chromatography-mass spectrometry (GC–MS) and high-performance liquid chromatography-mass spectrometry (HPLC–MS). Present observations confirmed that the catechin was biotransformed into 11 metabolites upon microbial dehydroxylation and C ring cleavage. Further, immunomodulatory potential of catechin metabolites was analyzed in peripheral blood mononuclear cells (PBMCs). We found up-regulation of anti-inflammatory cytokine (IL-4, IL-10) and down-regulation of pro-inflammatory (IL-16, IL-12B) cytokine may be due to Th2 immune response. In conclusion, biotransformed catechin metabolites enhance anti-inflammatory cytokines which is beneficial for overcoming inflammatory disorders.

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Effect in vitro of a bacterial extract (OM-89) on interleukin 1 and interleukin 2 production by peripheral blood mononuclear cells from healthy subjects and rheumatoid arthritis patients

International Journal of Immunopharmacology ◽

10.1016/0192-0561(90)90011-b ◽

1990 ◽

Vol 12 (8) ◽

pp. 909-913 ◽

Cited By ~ 3

Author(s):

Jacques Clot ◽

Monique Andary

Keyword(s):

Rheumatoid Arthritis ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Healthy Subjects ◽

Mononuclear Cells ◽

Interleukin 1 ◽

Interleukin 2 ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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Effects of a Hydrosoluble Bacterial Extract from Escherichia Coli (OM-89) on Cytokine Production by Peripheral Blood Mononuclear Cells from Healthy Subjects and Patients with Rheumatoid Arthritis

Scandinavian Journal of Rheumatology ◽

10.3109/03009749109103021 ◽

1991 ◽

Vol 20 (3) ◽

pp. 196-203 ◽

Cited By ~ 6

Author(s):

N. Franchimont ◽

Y. Vrindts-Gevaert ◽

J. Collette ◽

P. Franchimont

Keyword(s):

Rheumatoid Arthritis ◽

Escherichia Coli ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Healthy Subjects ◽

Mononuclear Cells ◽

Cytokine Production ◽

Peripheral Blood Mononuclear ◽

Bacterial Extract ◽

Blood Mononuclear Cells

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Lipopolysaccharide and silica-stimulated mononuclear cell prostaglandin production in ulcerative colitis

Mediators of Inflammation ◽

10.1080/09629350020002903 ◽

2000 ◽

Vol 9 (3-4) ◽

pp. 189-191

Author(s):

Neville A. Punchard ◽

John Cason ◽

Jonathan Mullins ◽

Chaman Chander ◽

Richard P. H. Thompson

Keyword(s):

Ulcerative Colitis ◽

Crohn’S Disease ◽

Mononuclear Cell ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Healthy Subjects ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Prostaglandin Production ◽

Blood Mononuclear Cells

Basal, lipopolysaccharide (LPS) and silica-stimulated prostaglandin (PG) production were compared between peripheral blood mononuclear cells (PBMNC) from UC patients and healthy subjects (HS). Basal and LPS-stimulated PBMNC PGI2, but not PGE2, production was greater in UC. LPS stimulated both PGE2and PGI2by PBMNC from HS and UC patients. Silica stimulated production of both PGs by cells from HS but only PGE2by cells from UC patients. The differences in responses to silica and LPS may result from differences in activation of NFκB or, alternatively, prior sensitisation to one of these agents. That PBMNC PGE2production is not increased in UC, as it is in Crohn’s disease, suggests that there are differences in PBMNC behaviour between these two diseases.

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