Comparative transcriptome and iTRAQ-based proteome analysis in mature leaves of Brassica carinata provides insights into the purple leaf color diversity

Abstract The leaf color of seedling populations derived from ornamental genotypes of Japanese barberry (Berberis thunbergii DC.) was evaluated to determine whether nursery selections of this important landscape plant could be expected to produce green-leaf progeny or seedlings with leaf color resembling the purple-leaf or yellow-leaf parent. This is a compelling inquiry since nearly all B. thunbergii plants found within invasive populations possess green foliage and the potential contribution of seedlings by ornamental purple-and yellow-leaf genotypes is unknown. Seed lots collected from cultivated barberry genotypes located in landscape settings were processed and raised in a greenhouse to observe leaf color phenotype. It was found that all genotypes studied produced at least some green seedlings. The percentage of green progeny produced varied widely by genotype. Green-leaf cultivars yielded close to 100% green seedlings and all purple-and yellow-leaf forms produced at least 20% green offspring. Among purple-leaf genotype accessions located adjacent to potential purple-leaf pollen donors, var. atropurpurea produced significantly fewer green seedlings (18.5%) than ‘Crimson Pygmy’ (71%) and ‘Rose Glow’ (45%). ‘Rose Glow’ individuals growing adjacent to other purple Japanese barberry forms produced significantly fewer green seedlings (45%) than ‘Rose Glow’ accessions that were isolated from additional purple Japanese barberry (88%). This study demonstrates that some invasive green-leaf B. thunbergii could be derived from popular garden forms since purple- and yellow-leaf genotypes readily produce green-leaf offspring which resemble feral barberry. These findings do not, however, provide any definitive link between cultivated and naturalized Japanese barberry.

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RNA-Seq-Based Profiling of pl Mutant Reveals Transcriptional Regulation of Anthocyanin Biosynthesis in Rice (Oryza sativa L.)

International Journal of Molecular Sciences ◽

10.3390/ijms22189787 ◽

2021 ◽

Vol 22 (18) ◽

pp. 9787

Author(s):

Ruonan Xu ◽

Ronghui Pan ◽

Yuchan Zhang ◽

Yanlei Feng ◽

Ujjal Kumar Nath ◽

...

Keyword(s):

Molecular Mechanisms ◽

Anthocyanin Biosynthesis ◽

Oryza Sativa L ◽

Transcriptional Profiling ◽

Expression Patterns ◽

Regulatory Gene ◽

Leaf Color ◽

Phenotypic Marker ◽

Bhlh Genes ◽

Purple Leaf

Purple-colored leaves in plants attain much interest for their important biological functions and could be a potential source of phenotypic marker in selecting individuals in breeding. The transcriptional profiling helps to precisely identify mechanisms of leaf pigmentation in crop plants. In this study, two genetically unlike rice genotypes, the mutant purple leaf (pl) and wild (WT) were selected for RNA-sequencing and identifying the differentially expressed genes (DEGs) that are regulating purple leaf color. In total, 609 DEGs were identified, of which 513 and 96 genes were up- and down-regulated, respectively. The identified DEGs are categorized into metabolic process, carboxylic acid biosynthesis, phenylpropanoids, and phenylpropanoid biosynthesis process enrichment by GO analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) confirmed their association with phenylpropanoid synthesis, flavonoid synthesis, and phenylalanine metabolism. To explore molecular mechanism of purple leaf color, a set of anthocyanin biosynthetic and regulatory gene expression patterns were checked by qPCR. We found that OsPAL (Os02g0626100, Os02g0626400, Os04g0518400, Os05g0427400 and Os02g0627100), OsF3H (Os03g0122300), OsC4HL (Os05g0320700), and Os4CL5 (Os08g0448000) are associated with anthocyanin biosynthesis, and they were up-regulated in pl leaves. Two members of regulatory MYB genes (OsMYB55; Os05g0553400 and Os08g0428200), two bHLH genes (Os01g0196300 and Os04g0300600), and two WD40 genes (Os11g0132700 and Os11g0610700) also showed up-regulation in pl mutant. These genes might have significant and vital roles in pl leaf coloration and could provide reference materials for further experimentation to confirm the molecular mechanisms of anthocyanin biosynthesis in rice.

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Comparative transcriptome analysis reveals candidate genes for leaf color formation in a thermo-sensitive leaf-color mutant generated by carbon-ion beam in green wandering Jew (Tradescantia fluminensis)

Scientia Horticulturae ◽

10.1016/j.scienta.2021.110300 ◽

2021 ◽

Vol 288 ◽

pp. 110300

Author(s):

Ruiyuan Liu ◽

Xicun Dong ◽

Miaomiao Zhang ◽

Ying Qu ◽

Wenjie Jin ◽

...

Keyword(s):

Candidate Genes ◽

Transcriptome Analysis ◽

Ion Beam ◽

Comparative Transcriptome ◽

Leaf Color ◽

Color Formation ◽

Color Mutant ◽

Carbon Ion Beam ◽

Tradescantia Fluminensis ◽

Leaf Color Mutant

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Physiological evaluation for salt tolerance in green and purple leaf color rice cultivars at seedling stage

Physiology and Molecular Biology of Plants ◽

10.1007/s12298-021-01114-y ◽

2021 ◽

Author(s):

Noppawan Nounjan ◽

Piyada Theerakulpisut

Keyword(s):

Salt Tolerance ◽

Seedling Stage ◽

Rice Cultivars ◽

Leaf Color ◽

Physiological Evaluation ◽

Purple Leaf

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Comparative Transcriptome Analysis of Young and Mature Leaves of Taraxacum kok-saghyz L. Rodin

Botanical Research ◽

10.12677/br.2021.102013 ◽

2021 ◽

Vol 10 (02) ◽

pp. 85-96

Author(s):

全亮谢

Keyword(s):

Transcriptome Analysis ◽

Comparative Transcriptome ◽

Comparative Transcriptome Analysis ◽

Mature Leaves

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Comparative transcriptome and proteome analysis to reveal the biosynthesis of gold nanoparticles in Arabidopsis

Scientific Reports ◽

10.1038/srep21733 ◽

2016 ◽

Vol 6 (1) ◽

Cited By ~ 14

Author(s):

Manish Tiwari ◽

Sneha Krishnamurthy ◽

Devesh Shukla ◽

Jeffrey Kiiskila ◽

Ajay Jain ◽

...

Keyword(s):

Gold Nanoparticles ◽

Proteome Analysis ◽

Comparative Transcriptome

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Upregulation of the MYB2 Transcription Factor is Associated with Increased Accumulation of Anthocyanin in the Leaves of Dendrobium bigibbum

International Journal of Molecular Sciences ◽

10.3390/ijms21165653 ◽

2020 ◽

Vol 21 (16) ◽

pp. 5653

Author(s):

Gah-Hyun Lim ◽

Se Won Kim ◽

Jaihyunk Ryu ◽

Si-Yong Kang ◽

Jin-Baek Kim ◽

...

Keyword(s):

Biosynthetic Pathway ◽

Anthocyanin Biosynthesis ◽

Leaf Color ◽

Γ Irradiation ◽

Expression Of Genes ◽

Normal Green ◽

Purple Coloration ◽

Transient Overexpression ◽

High Level ◽

Purple Leaf

Orchids with colorful leaves and flowers have significant ornamental value. Here, we used γ-irradiation-based mutagenesis to produce a Dendrobium bigibbum mutant that developed purple instead of the normal green leaves. RNA sequencing of the mutant plant identified 2513 differentially expressed genes, including 1870 up- and 706 downregulated genes. The purple leaf color of mutant leaves was associated with increased expression of genes that encoded key biosynthetic enzymes in the anthocyanin biosynthetic pathway. In addition, the mutant leaves also showed increased expression of several families of transcription factors including the MYB2 gene. Transient overexpression of D. biggibumMYB2 in Nicotiana benthamiana was associated with increased expression of endogenous anthocyanin biosynthesis genes. Interestingly, transient overexpression of orthologous MYB2 genes from other orchids did not upregulate expression of endogenous anthocyanin biosynthesis genes. Together, these results suggest that the purple coloration of D. biggibum leaves is at least associated with increased expression of the MYB2 gene, and the MYB2 orthologs from orchids likely function differently, regardless of their high level of similarity.

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