Is the body composition development in premature infants associated with a distinctive nuclear magnetic resonance metabolomic profiling of urine?

2018 ◽  
Vol 32 (14) ◽  
pp. 2310-2318 ◽  
Author(s):  
Daniela Morniroli ◽  
Angelica Dessì ◽  
Maria Lorella Giannì ◽  
Paola Roggero ◽  
Antonio Noto ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Body Composition ◽  
Magnetic Resonance ◽  
Premature Infants ◽  
The Body ◽  
Metabolomic Profiling ◽  
Nuclear Magnetic

scholarly journals Verification of precision, accuracy, and characteristics of dual-energy X-ray absorptiometry and nuclear magnetic resonance in the analysis of mouse body composition

2020 ◽  
Author(s):  
Kyung-Wan Baek ◽  
Ji-Seok Kim ◽  
Jin Sung Park ◽  
So-Jeong Kim ◽  
Yong-Chan Ha ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Body Composition ◽  
Magnetic Resonance ◽  
Dual Energy ◽  
The Body ◽  
Experimental Animals ◽  
X Ray ◽  
Advantages And Disadvantages ◽  
Measurement Efficiency ◽  
Nuclear Magnetic

Abstract Background: As an instrument for measuring body composition in experimental animals, dual-energy X-ray absorptiometry (DXA) is ideal for accuracy, cost, and measurement efficiency. However, there is too little insight into the effectiveness of the various aspects of applying DXA to experimental animals. Therefore, we investigated whether to compare and verify the precision and accuracy of DXA and nuclear magnetic resonance (NMR) animal body composition analyzers. We used 30 ICR mice in the study. First, in order to evaluate the reproducibility of DXA and NMR, we did repeated measurements by repositioning each mouse in anesthesia and euthanasia states. Subsequently, the accuracy of each device was evaluated by comparing the weight measured before the experiment, the weight of the tissue extracted from the mice after the experiment, and the measured DXA and NMR. In addition, when measuring the body composition of animals, we compared the time and the measurable body composition parameters and summarized the advantages and disadvantages of the two devices.Results: Compared to NMR, DXA had the advantage of a fast measurement of bone composition and rapid image analysis. In addition, DXA showed a higher correlation (> 95%) with FM, body weight, and fBMC baseline than did NMR (> 85%).Conclusion: In conclusion, DXA was confirmed to have higher precision and measurement accuracy than did NMR. Therefore, DXA is an effective method for evaluating the body composition of experimental animals.

scholarly journals Development of an optimized method for processing peripheral blood mononuclear cells for 1H-nuclear magnetic resonance-based metabolomic profiling

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247668
Author(s):  
León Gabriel Gómez-Archila ◽  
Martina Palomino-Schätzlein ◽  
Wildeman Zapata-Builes ◽  
Elkin Galeano
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Metabolomic Profiling ◽  
Blood Mononuclear Cells ◽  
Nuclear Magnetic

Human peripheral blood mononuclear cells (PBMCs) are part of the innate and adaptive immune system, and form a critical interface between both systems. Studying the metabolic profile of PBMC could provide valuable information about the response to pathogens, toxins or cancer, the detection of drug toxicity, in drug discovery and cell replacement therapy. The primary purpose of this study was to develop an improved processing method for PBMCs metabolomic profiling with nuclear magnetic resonance (NMR) spectroscopy. To this end, an experimental design was applied to develop an alternative method to process PBMCs at low concentrations. The design included the isolation of PBMCs from the whole blood of four different volunteers, of whom 27 cell samples were processed by two different techniques for quenching and extraction of metabolites: a traditional one using organic solvents and an alternative one employing a high-intensity ultrasound probe, the latter with a variation that includes the use of deproteinizing filters. Finally, all the samples were characterized by 1H-NMR and the metabolomic profiles were compared by the method. As a result, two new methods for PBMCs processing, called Ultrasound Method (UM) and Ultrasound and Ultrafiltration Method (UUM), are described and compared to the Folch Method (FM), which is the standard protocol for extracting metabolites from cell samples. We found that UM and UUM were superior to FM in terms of sensitivity, processing time, spectrum quality, amount of identifiable, quantifiable metabolites and reproducibility.

scholarly journals A noninvasive method to study regulation of extracellular fluid volume in rats using nuclear magnetic resonance

2016 ◽  
Vol 310 (5) ◽  
pp. F426-F431 ◽  
Author(s):  
Christopher J. Gordon ◽  
Pamela M. Phillips ◽  
Andrew F. M. Johnstone
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Body Composition ◽  
Magnetic Resonance ◽  
Extracellular Volume ◽  
Isotonic Saline ◽  
Extracellular Fluid ◽  
Free Water ◽  
Sprague Dawley ◽  
Wistar Kyoto ◽  
Nuclear Magnetic

Time-domain nuclear magnetic resonance (TD-NMR)-based measurement of body composition of rodents is an effective method to quickly and repeatedly measure proportions of fat, lean, and fluid without anesthesia. TD-NMR provides a measure of free water in a living animal, termed %fluid, and is a measure of unbound water in the vascular and extracellular spaces. We hypothesized that injecting a bolus of fluid into the peritoneal cavity would lead to an abrupt increase in %fluid and the rate of clearance monitored with TD-NMR would provide a noninvasive assessment of the free water homeostasis in an awake rat. Several strains of laboratory rats were injected intraperitoneally with 10 ml/kg isotonic or hypertonic saline and %fluid was monitored repeatedly with a Bruker “Minispec” TD-NMR body composition system. Following isotonic saline, %fluid increased immediately by 0.5% followed by a recovery over ∼6 h. Injecting hypertonic (3 times normal saline) resulted in a significantly greater rise in %fluid and longer recovery. Intraperitoneal and subcutaneous fluid injection led to similar rates of clearance. The Wistar-Kyoto rat strain displayed significantly slower recovery to fluid loads compared with Long-Evans and Sprague-Dawley strains. Rats exercised chronically showed significant increases in %fluid, but the rate of clearance of fluid was similar to that of sedentary animals. We conclude that this technique could be used to study vascular and extracellular volume homeostasis noninvasively in rats.

scholarly journals Nuclear magnetic resonance metabolomic profiling of urine provides a noninvasive alternative to the identification of biomarkers associated with endometriosis

2015 ◽  
Vol 104 (5) ◽  
pp. 1202-1209 ◽  
Author(s):  
Sara Vicente-Muñoz ◽  
Inmaculada Morcillo ◽  
Leonor Puchades-Carrasco ◽  
Vicente Payá ◽  
Antonio Pellicer ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Metabolomic Profiling ◽  
Nuclear Magnetic

scholarly journals Serum metabolite profiles of habitual diet: evaluation by 1H-nuclear magnetic resonance analysis

2019 ◽  
Vol 110 (1) ◽  
pp. 53-62 ◽  
Author(s):  
Helen M Lindqvist ◽  
Millie Rådjursöga ◽  
Daniel Malmodin ◽  
Anna Winkvist ◽  
Lars Ellegård
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Body Composition ◽  
Magnetic Resonance ◽  
1H Nmr ◽  
Food Diary ◽  
Serum Samples ◽  
Orthogonal Projections ◽  
Animal Products ◽  
1H Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

ABSTRACTBackgroundObjective and reliable methods to measure dietary exposure and prove associations and causation between diet and health are desirable.ObjectiveThe aim of this study was to investigate if 1H-nuclear magnetic resonance (1H-NMR) analysis of serum samples may be used as an objective method to discriminate vegan, vegetarian, and omnivore diets. Specifically, the aim was to identify a metabolite pattern that separated meat-eaters from non–meat-eaters and vegans from nonvegans.MethodsHealthy volunteers (45 men and 75 women) complying with habitual vegan (n = 43), vegetarian (n = 24 + vegetarians adding fish n = 13), or omnivore (n = 40) diets were enrolled in the study. Data were collected on clinical phenotype, body composition, lifestyle including a food-frequency questionnaire (FFQ), and a 4-d weighed food diary. Serum samples were analyzed by routine clinical test and for metabolites by 1H-NMR spectroscopy. NMR data were nonnormalized, UV-scaled, and analyzed with multivariate data analysis [principal component analysis, orthogonal projections to latent structures (OPLS) and OPLS with discriminant analysis]. In the multivariate analysis volunteers were assigned as meat-eaters (omnivores), non–meat-eaters (vegans and vegetarians), vegans, or nonvegans (lacto-ovo-vegetarians, vegetarians adding fish, and omnivores). Metabolites were identified by line-fitting of 1D 1H-NMR spectra and the use of statistical total correlation spectroscopy.ResultsAlthough many metabolites differ in concentration between men and women as well as by age, body mass index, and body composition, it was possible to correctly classify 97.5% of the meat-eaters compared with non–meat-eaters and 92.5% of the vegans compared with nonvegans. The branched-chain amino acids, creatine, lysine, 2-aminobutyrate, glutamine, glycine, trimethylamine, and 1 unidentified metabolite were among the most important metabolites in the discriminating patterns in relation to intake of both meat and other animal products.Conclusions1H-NMR serum metabolomics appears to be a possible objective tool to identify and predict habitual intake of meat and other animal products in healthy subjects. These results should be confirmed in larger cohort studies or intervention trials. This trial was registered at clinicaltrials.gov as NCT02039609.

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