GC-MS and LC-MS Identification of the Phenolic Compounds Present in the ethyl Acetate Fraction Obtained from Senna tora, L. Roxb. seeds

2018 ◽  
Vol 33 (19) ◽  
pp. 2878-2881
Author(s):  
Noha Fathalla ◽  
Mokhtar Bishr ◽  
Abdel Nasser Singab ◽  
Osama Salama
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction

scholarly journals PHYTOCHEMICAL SCREENING AND BIOLOGICAL EVALUATION OF DYPSIS LEPTOCHEILOS LEAVES EXTRACT AND MOLECULAR DOCKING STUDY OF THE ISOLATED COMPOUNDS

2020 ◽  
pp. 106-113
Author(s):  
HAITHAM ALI IBRAHIM ◽  
FATEHIA SAYED ELSHARAWY ◽  
MAHMMOUD ELHASSAB ◽  
SAMAH SHABANA ◽  
EMAN GABER HAGGAG
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Chromatographic Separation ◽  
Ethyl Acetate Fraction ◽  
Docking Study ◽  
Separation Techniques ◽  
Reference Drug ◽  
First Time ◽  
Mcf 7

Objective: phytochemical investigation of the ethyl acetate fraction (EAF) of 80% aqueous methanol extract (AME) of Dypsis leptocheilos leaves, in addition to evaluation of the antioxidant, cytotoxic and antimicrobial activities of the AME and EAF. Docking was used to predict and understand cytotoxicity of the isolated compounds. Methods: The ethyl acetate fraction (EAF) of Dypsis leptocheilos leaves was subjected to different chromatographic separation techniques. Structures of the isolated compounds were established by different spectroscopic techniques (1H/13C NMR). Antioxidant activity was evaluated by DPPH assay, while cytotoxicity was evaluated by MTT cell viability assay. Antimicrobial activity was evaluated by agar diffusion method. The docking study was conducted using Auto Dock Vina; the estrogen receptor (PDB 5t92) was used as a receptor for the docking. Results: Chromatographic separation techniques were led to the isolation of five phenolic compounds; these compounds were identified to be apigenin 8-C-β-D-glucopyranoside (Vitexin) (1), apigenin 6-C-β-D-glucopyranoside (Isovitexin) (2), luteolin 7-O-β-D-glucopyranoside (3), luteolin 8-C-β-D-glucopyranoside (Orientin) (4), luteolin 6-C-β-D-glucopyranoside (Isoorientin) (5). They were isolated and identified for the first time from this plant species. The AME and EAF showed moderate activity against Gram positive and Gram negatvie bacteria, while both of them showed similar and powerful antioxidant activity with SC50 = 12.8±0.56 µg/ml and SC50 = 17±0.77 µg/ml respectively, compared to ascorbic (reference drug) SC50 = 14.2±0.35 µg/ml. The EAF showed higher cytotoxic activity on the MCF-7 cells (human breast cancer cell line), with IC50 = 12.3 ± 1.82 µg/ml, compared to Vinblastine Sulfate (reference drug). All isolated compounds showed good binding affinity to the estrogen receptors existed in the MCF-7 cell. Conclusion: Five phenolic compounds were isolated for the first time from the EAF of Dypsis leptocheilos leaves. The AME and EAF extracts showed variable antioxidant, antimicrobial and cytotoxic activities.

scholarly journals Phenolic composition and antioxidant potential of different solvent extracts of the endemic Origanum elongatum (Bonnet) Emberger& Maire

2020 ◽  
Vol 10 (2) ◽  
pp. 146-154
Author(s):  
Imane Tagnaout ◽  
Hannou Zerkani ◽  
Sanae Amine ◽  
Kamal Fadili ◽  
Nadia Benhlima ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Screening Tests ◽  
Dpph Radical ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Health Disorders ◽  
Hydroethanolic Extract

Plants of the Lamiaceae family are widely used for medicinal, cosmetic and culinary purposes. Phenolic compounds such as flavonoids and tannins are the main constituents of these plants; they have a proven multidirectional biological activity. Polyphenols have exceptional antioxidant potential, and they can intervene in the prevention of many health disorders. This work aims to study the composition and antioxidant power of phenolic compounds from crude extracts and fractions of Origanum elongatum leaves. This specie was subjected to phytochemical study through precipitation, turbidity and coloring reactions to highlight their secondary metabolites qualitative composition. Polyphenolic extraction was made by Soxhlet using two solvents: methanol 70%, ethanol 70%. Then, methanol’s crude was fractionated with solvents having escalating polarity; ethyl acetate, n-butanol. The polyphenols and flavonoids contents were spectrophotometrically estimated using Folin-Ciocalteau and Aluminum Trichloride methods. The antioxidant power of crude and fractionated extracts was evaluated by diphenyl-picryl-hydrazyl (DPPH●) and iron (FRAP) tests. The screening tests showed the existence of polyphenols, mucilages, sterols and triterpenes, oses and holosides. The highest yield of extraction is obtained by 70% methanol, with an average of 32.29%. Quantitative assays indicated that the hydroethanolic extract and the ethyl acetate fraction possessed high amount of polyphenols and flavonoids compared with other fractions and subsequently exhibited a marked scavenging activity on DPPH radical (IC50 = 0,085±0,002 and 0,112±0,003 mg/ml, respectively) and high reductive ability on ferric ion assay with IC50 = 0,181±0,004 mg/ml, 0,291±0,005 mg/ml, respectively.

scholarly journals Inhibition of Alpha-Amylase Activity by Gymnocarpos decandrus Forssk. Constituents

2017 ◽  
Vol 9 (6) ◽  
Author(s):  
Amal Sallam ◽  
Amal A Galala
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Inhibitory Activity ◽  
Methylene Chloride ◽  
Amylase Activity ◽  
Ethyl Acetate Fraction ◽  
2D Nmr ◽  
Wild Plant ◽  
Spectroscopic Techniques ◽  
Obesity And Diabetes

The methanolic extract of the aerial parts of Gymnocarpos decandrus, the plant growing wildly in the middle east area and north Africa, showed a promising -amylase inhibitory activity. The ethyl acetate fraction of the plant showed the highest inhibitory activity followed by the methylene chloride fraction. Phytochemical investigation of both fractions led to the isolation of 10 compounds; oleanolic acid (1), maslinic acid (2), apigenin (3), β-sitosterol glucoside (4), luteolin (5), afzelechin (6), epiafzelechin (7), catechin (8), epicatechin (9) and gallocatechin (10). The structures of compounds 1-10 were established on the basis of extensive 1D- and 2D-NMR spectroscopic techniques. This is the first report for the isolation of these compounds from G. decandrus. The phenolic compounds (6-10) isolated from the ethyl acetate fraction showed the highest -amylase inhibitory activity. The more the hydroxylation pattern of the isolated phenolic compounds (6, 8, 9 and 10), the higher the ability to inhibit the -amylase activity. This was confirmed through the molecular docking experiment. The wild plant G. decandrus is a promising -amylase inhibitor that can be used in the management of obesity and diabetes mellitus.

Antioxidant Potential and Total Phenolic Compounds of Extracts and Fractions of Pistasia atlantica

2017 ◽  
Vol 9 (04) ◽  
Author(s):  
Somayeh Alidadi ◽  
Mohammad-Taghi Moradi ◽  
Majid Asadi-Samani ◽  
Zahra Lorigooini
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Total Phenolic ◽  
Chloroform Fraction ◽  
Total Phenolic Compounds ◽  
Alcohol Extract ◽  
Ic50 Value

Different parts of Pistasia atlantica have been used in traditional medicine for various purposes in Iran. The aim of this study was to measurement and compare antioxidant activity and polyphenolic compounds of crude ethyl alcohol extract and four fractions of P. atlantica leaf. Crude ethyl alcohol extract of P. atlantica leaf was prepared using maceration method and subjected to fractionation with different polarity. The antioxidant potential of all these fractions was evaluated by the 2,2 diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity method. The total phenolic, flavonoid, and flavonol components were measured with Folin-Ciocaltiue and Chlorid Aluminum methods. According to the radical scavenging capacity, the ethyl acetate fraction exhibited the highest antioxidant activity with IC50 value 1.54±0.12 µg/ml, followed by the chloroform fraction with higher percent inhibition of the DPPH with 3.4±0.11 µg/ml. The results are represented relative to a reference standard, butylated hydroxytoluene (BHT), with IC50 value of 33.5±3.67μg/ml. Among these fractions, the ethyl acetate fraction and chloroform fraction had the highest amount of total phenolic compounds with value of 532.73 and 355.14 mg GAE/g, respectively. The results of this study showed that some fractions of P. atlantica leaf extract could be used as easily accessible source of natural antioxidants

scholarly journals Determination of Phenolic Compounds in Saussurea salicifolia (L.) DC. by HPLC

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S259-S261 ◽  
Author(s):  
G. Chunsriimyatav ◽  
I. Hoza ◽  
P. Valášek ◽  
S. Skrovanková ◽  
D. Banzragch ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Flow Rate ◽  
Mobile Phase ◽  
Hplc Analysis ◽  
Ethyl Acetate Fraction ◽  
Flavonoid Aglycones ◽  
Calibration Curves ◽  
Flavonoid Aglycone

The flavonoid aglycone content of ethyl acetate fraction of <I>Saussurea salicifolia</I> (L.) DC was analysed by the HPLC “Agilent” 1100 with Alltech C18 &mu;m column, UV254 detector, flow rate: 1 ml/min, mobile phase: acetonitrile: water:acetic acid (36:65:1). Eleven flavonoid aglycones in the ethyl acetate fraction of <I>Saussurea salicifolia</I> were detected by HPLC analysis. The quantification of each compound was done according to the peak area measurements which were reported in calibration curves of the corresponding standards. The result revealed that <I>Saussurea salicifolia</I> consists of flavonoid aglycones of quercetin (28.62%) and kaempferol (3.01%).

Characterisation of phenolic compounds of the ethyl acetate fraction from Tabernaemontana catharinensis and its potential antidepressant-like effect

2017 ◽  
Vol 32 (16) ◽  
pp. 1987-1990 ◽  
Author(s):  
Nathielli Nayara Pauleti ◽  
Jonas Mello ◽  
Diogo Alexandre Siebert ◽  
Gustavo Amadeu Micke ◽  
Cláudia Almeida Coelho de Albuquerque ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction

scholarly journals Aktivitas Koagulan Ekstrak dan Fraksi Daun Sembung Rambat (Mikania micrantha Kunth.)

2019 ◽  
Vol 4 (1) ◽  
pp. 30-37
Author(s):  
Santi Perawati ◽  
Lili Andriani ◽  
Septa Pratama ◽  
Humayroh Humayroh
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Key Words ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Mikania Micrantha ◽  
Climbing Plant ◽  
Liquid Extract ◽  
Stop Time ◽  
Coagulant Activity

ABSTRAK Sembung Rambat (Mikania micrantha Kunth) merupakan tumbuhan yang tumbuh merambat mengandung zat aktif dalam bentuk metabolit sekunder seperti alkaloid, saponin, flavonoid, steroid, tanin, terpenoid dan kaya akan senyawa fenolik. Penelitian ini dilakukan untuk mengetahui kemampuan ekstrak dan fraksi dari daun sembung rambat sebagai koagulan. Ekstraksi daun segar sembung rambat menggunakan metode maserasi dengan pelarut etanol 96% , ekstrak etanol difraksinasi menggunakan metode ekstrak cair-cair dengan pelarut n-heksan, etil asetat dan n-butanol. Aktivitas koagulan diukur dengan cara melihat waktu terbentuk benang fibrin dan waktu henti darah pada hewan uji Mencit Putih Galur (Deutschland, Denkenand and Yoken). Ekstrak dengan dosis 60mg/20g BB mencit menunjukkan hasil aktivitas koagulan yang terbaik dibandingkan dengan variasi dosis lain dengan waktu terbentuk benang fibrin (77 detik) dan waktu henti darah (86 detik). Pada fraksi etil asetat memiliki aktivitas koagulan terbaik dibandingkan dengan ekstrak dan fraksi lain dengan waktu terbentuk benang fibrin (64 detik) dan waktu henti darah (86 detik). Ekstrak dan fraksi daun sembung rambat pada dosis 60mg/20g BB mencit memiliki aktivitas koagulan yang baik. Kata kunci: Koagulan, Sembung Rambat (Mikania micrantha Kunth.)     ABSTRACT Sembung Rambat (Mikania micrantha Kunth.) Is a climbing plant containing alkaloids, saponins, flavonoids, steroids, tannins, terpenoids and rich in phenolic compounds. This aim was to determine the ability of extracts and fractions of sembung rambat leaves as coagulant. Extraction of fresh leaves of used maceration method with ethanol 96% as a solvent, ethanol extract fractionated used liquid-liquid extract method with n-hexane, ethyl acetate and n-butanol. Coagulant activity was measured by looking at the time of formation of fibrin yarn and the time of blood stops in theanimals test of White Mice (Deutschland, Denkenand and Yoken). Extracts with a dose of 60 mg / 20 g BB mice showed the best results of coagulant activity compared to other dose variations with the time of formation of fibrin yarn 77 seconds and blood stop time 86 seconds. The ethyl acetate fraction has the best coagulant activity compared to extracts and other fractions with fibrin yarn formed time 64 seconds and blood stop time 86 seconds. In conclusion, the extracts and fractions of sembung rambat have coagulant activity.  Key words: Coagulant, Mikania micrantha Kunth

scholarly journals Identification and antioxidant activity of Ammi visnaga L. polyphenols from the Middle Atlas in Morocco

2020 ◽  
Vol 10 (7) ◽  
pp. 649
Author(s):  
Jamila El Karkouri ◽  
Aziz Drioiche ◽  
Aminata Soro ◽  
Atika Ailli ◽  
Nadia Benhlima ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Biochanin A ◽  
Total Polyphenols ◽  
Ammi Visnaga ◽  
Liquid Liquid Extraction ◽  
Middle Atlas

<p>In this work, we are interested in both the study of the phenolic compounds and the evaluation of the antioxidant properties of the extracts of <em>Ammi visnaga L. </em>collected from the Middle Atlas in Morocco. Phytochemical screening was carried out to highlight the qualitative composition of the secondary metabolites contained in this plant. Also, the extraction of total polyphenols was carried out by maceration using hydromethanolic and hydroacetonic mixtures. Then, the fractionation of the crude extracts was conducted by liquid-liquid extraction using successively two organic solvents with different polarities: ethyl acetate and n-butanol. The polyphenols contents were spectrophotometrically estimated using the Folin-Ciocalteau method. It showed that the ethyl acetate fraction is more abundant in phenolic compounds than the other fractions. The qualitative analysis was performed by high-pressure liquid chromatography with U.V. and electrospray ionization coupled to mass spectrometry (HPLC/UV-ESI-MS), which confirmed the richness of this species in polyphenols and particularly in flavonoids, especially by the identification of kaempferol, rhamnetin, biochanin A, ammiol. These molecules belong to flavones, flavonols, and furochromones, which are also identified both in the form of genin and of heteroside.<strong> </strong>The antioxidant activity of the different fractions was evaluated by diphenyl-picryl-hydrazyl (DPPH<sup>●</sup>). The IC<sub>50</sub> is equal to 12.87 μg / mL for the ethyl acetate fraction, against 8.8 μg / mL for ascorbic acid and 10.8 μg / mL for BHA which are used as reference compounds.</p>

Phenolic compounds from Andean mashua (Tropaeolum tuberosum) tubers display protection against soybean oil oxidation

2012 ◽  
Vol 18 (3) ◽  
pp. 271-280 ◽  
Author(s):  
I Betalleluz-Pallardel ◽  
R Chirinos ◽  
H Rogez ◽  
R Pedreschi ◽  
D Campos
Keyword(s):  
Phenolic Compounds ◽  
Soybean Oil ◽  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Butylated Hydroxytoluene ◽  
Protective Effects ◽  
Oil Oxidation ◽  
Scanning Calorimetry ◽  
Aqueous Fraction ◽  
Accelerated Storage

Phenolic compounds from mashua tuber were evaluated as potential antioxidants to retard the oxidation of crude soybean oil submitted to accelerated storage and frying. During the accelerated storage, an ethanolic crude extract, a purified extract, an aqueous fraction and an ethyl acetate fraction from mashua containing different gallic acid equivalent concentrations (100, 300 and 600 ppm) in oil were evaluated at 55 °C. After 15 days of storage, better effects were evidenced against soybean oil oxidation at 300 and 600 ppm of ethyl acetate fraction in comparison to 200 ppm butylated hydroxytoluene and the control (no antioxidant added). During the frying process at ∼180 °C, principal component analysis revealed that the content of trienes and dienes were strongly correlated with the frying batch. Ethyl acetate fraction at 200 ppm showed the highest efficacy against oil oxidation in terms of polar compound values, free fatty acids and conjugated dienes and trienes in comparison to the oil containing 200 ppm tert-butylhydroquinone and control. Differential scanning calorimetry corroborated the efficacy of ethyl acetate fraction phenolic and it is strongly recommended as method for validation of results. This study provides strong evidence related to the excellent protective effects against soybean oil oxidation of mashua phenolics. This crop could be utilized as an alternative source of natural antioxidants by the oil industry.

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